Rh2DG180500

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001289
Physical Location & Seq
Reverse (-)
15906794 .. 15908158
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG180500.1

Sequence Viewer

Length: 798 bp
ATGTCCATTGGTTTGGCAAAGAGAAAGATGGTGACAGTTCTGAGCATTGATGGAGGTGGCATTAGAGGCATAATTCCCGGCACCATCCTCGGCTTTCTCGAATCTAAGCTTCAGGATTGGGATGGAGCAAATGCAAGAATTGCAGATTATTTTGACATAATCGCCGGAACTAGCACCGGGGGATTGGTCACCACCATGCTTACAGCTCCCAGTAAGAACAACCGGCCCATGTATGAAGCAAAGGACATCAACAACTTTTATTTAGGACACTGTCCCCAGATTTTCCCTCAAAAAAGTCGGAAAAAGAAAAACTTCTTCACATCGATAACAAATGCGATGAAGCGGGGGCCAAAATACGATGGGATGTATCTGCGCTCATTGATAAACGGGCTCCTTGGTGATCTCACTCTCAAGCAGACTTTAACCAATGTGGTCATTCCAAGTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCTCAACCATCGATGCAAAAGAAAATTCATTGAAGAACGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATATTATCTCCCTCCACACTACTTCGAAATCAAGGATGACCAGGGAAATATCCGCAAATTTGATCTTGTCGATGTAATTTACCGTAGTGCTGCAATTAAGGAACTAATGCATGGATTTTATTCCATGAATGAAAGACAATGCCATAAGCCACATAAACAGAGAGATGTTGAAGAACTACTCAGAACCAATAAATGCGAATCGCTTGCAAGTGCTGTCATTGGGCACTGGCGCTGCCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

265

Amino Acids

29.74

Weight (kDa)

9.33

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 194 1.1e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AciI CCGC 2 cut(s) 343, 607
AcsI RAATTY 2 cut(s) 499, 611
AcuI CTGAAG 2 cut(s) 95, 446
AfaI GTAC 1 cut(s) 541
AgsI TTSAA 2 cut(s) 508, 725
AjnI CCWGG 1 cut(s) 594
AluBI AGCT 4 cut(s) 109, 206, 520, 551
AluI AGCT 4 cut(s) 109, 206, 520, 551
AoxI GGCC 2 cut(s) 224, 347
ApeKI GCWGC 3 cut(s) 548, 644, 786
ApoI RAATTY 2 cut(s) 499, 611
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 2 cut(s) 375, 786
AspS9I GGNCC 2 cut(s) 225, 347
AsuC2I CCSGG 2 cut(s) 78, 178
AsuHPI GGTGA 3 cut(s) 43, 181, 410
AsuII TTCGAA 1 cut(s) 579
BaeGI GKGCMC 1 cut(s) 780
BanI GGYRCC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 393
BbvI GCAGC 3 cut(s) 560, 631, 773
BccI CCATC 6 cut(s) 22, 44, 92, 116, 353, 491
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 740, 774
BciT130I CCWGG 1 cut(s) 596
BcnI CCSGG 2 cut(s) 78, 178
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 546
BfoI RGCGCY 1 cut(s) 787
BisI GCNGC 3 cut(s) 549, 645, 787
BlsI GCNGC 3 cut(s) 550, 646, 788
Bme1390I CCNGG 3 cut(s) 78, 178, 596
BmgT120I GGNCC 2 cut(s) 225, 347
BmiI GGNNCC 3 cut(s) 82, 348, 392
BmrFI CCNGG 3 cut(s) 78, 178, 596
BmrI ACTGGG 1 cut(s) 204
BmsI GCATC 2 cut(s) 478, 543
BmuI ACTGGG 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 78, 178
Bsa29I ATCGAT 2 cut(s) 323, 486
BsaJI CCNNGG 4 cut(s) 88, 177, 394, 595
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 2 cut(s) 210, 785
BseBI CCWGG 1 cut(s) 596
BseCI ATCGAT 2 cut(s) 323, 486
BseDI CCNNGG 4 cut(s) 88, 177, 394, 595
BseGI GGATG 4 cut(s) 84, 127, 369, 595
BseMII CTCAG 2 cut(s) 32, 748
BseNI ACTGG 2 cut(s) 210, 785
BseSI GKGCMC 1 cut(s) 780
BseXI GCAGC 3 cut(s) 560, 631, 773
BshFI GGCC 2 cut(s) 226, 349
BshNI GGYRCC 1 cut(s) 80
BshVI ATCGAT 2 cut(s) 323, 486
BsiSI CCGG 4 cut(s) 78, 165, 177, 223
BslFI GGGAC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 258
BsnI GGCC 2 cut(s) 226, 349
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 2 cut(s) 393, 780
Bsp143I GATC 2 cut(s) 400, 616
BspACI CCGC 2 cut(s) 343, 607
BspANI GGCC 2 cut(s) 226, 349
BspCNI CTCAG 2 cut(s) 33, 747
BspDI ATCGAT 2 cut(s) 323, 486
BspLI GGNNCC 3 cut(s) 82, 348, 392
BspMAI CTGCAG 1 cut(s) 550
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 80
BsrFI RCCGGY 1 cut(s) 222
BsrI ACTGG 2 cut(s) 210, 785
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 4 cut(s) 88, 177, 394, 595
BssMI GATC 2 cut(s) 400, 616
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 3 cut(s) 37, 272, 638
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 1 cut(s) 759
BstDEI CTNAG 3 cut(s) 41, 105, 734
BstEII GGTNACC 1 cut(s) 187
BstF5I GGATG 4 cut(s) 84, 127, 369, 595
BstH2I RGCGCY 1 cut(s) 787
BstHHI GCGC 2 cut(s) 375, 786
BstKTI GATC 2 cut(s) 403, 619
BstMBI GATC 2 cut(s) 400, 616
BstMWI GCNNNNNNNGC 2 cut(s) 66, 140
BstNI CCWGG 1 cut(s) 596
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 76, 176, 594
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 780
BstV1I GCAGC 3 cut(s) 560, 631, 773
BstXI CCANNNNNNTGG 1 cut(s) 13
Bsu15I ATCGAT 2 cut(s) 323, 486
BsuRI GGCC 2 cut(s) 226, 349
BsuTUI ATCGAT 2 cut(s) 323, 486
BtgZI GCGATG 1 cut(s) 350
BtsCI GGATG 4 cut(s) 84, 127, 369, 595
BtsIMutI CAGTG 2 cut(s) 268, 778
Cac8I GCNNGC 1 cut(s) 759
CfoI GCGC 2 cut(s) 375, 786
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 225, 347
ClaI ATCGAT 2 cut(s) 323, 486
Csp6I GTAC 1 cut(s) 540
CviAII CATG 4 cut(s) 196, 229, 665, 679
CviQI GTAC 1 cut(s) 540
DdeI CTNAG 3 cut(s) 41, 105, 734
DpnI GATC 2 cut(s) 402, 618
DpnII GATC 2 cut(s) 400, 616
Eco130I CCWWGG 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 95, 446
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 594
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 4 cut(s) 199, 232, 668, 682
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 1 cut(s) 258
FatI CATG 4 cut(s) 195, 228, 664, 678
FauI CCCGC 1 cut(s) 336
Fnu4HI GCNGC 3 cut(s) 549, 645, 787
FokI GGATG 4 cut(s) 71, 134, 376, 602
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 3 cut(s) 549, 645, 787
FspBI CTAG 1 cut(s) 171
GlaI GCGC 2 cut(s) 374, 785
GluI GCNGC 3 cut(s) 549, 645, 787
HaeII RGCGCY 1 cut(s) 787
HaeIII GGCC 2 cut(s) 226, 349
HapII CCGG 4 cut(s) 78, 165, 177, 223
HhaI GCGC 2 cut(s) 375, 786
Hin1II CATG 4 cut(s) 199, 232, 668, 682
Hin6I GCGC 2 cut(s) 373, 784
HinP1I GCGC 2 cut(s) 373, 784
HindIII AAGCTT 2 cut(s) 107, 518
HinfI GANTC 2 cut(s) 101, 752
HpaII CCGG 4 cut(s) 78, 165, 177, 223
HphI GGTGA 3 cut(s) 43, 181, 410
Hpy188I TCNGA 4 cut(s) 42, 300, 526, 737
Hpy188III TCNNGA 2 cut(s) 98, 113
HpyAV CCTTC 1 cut(s) 470
HpyCH4III ACNGT 3 cut(s) 37, 272, 638
HpyCH4V TGCA 8 cut(s) 134, 143, 491, 534, 548, 647, 664, 761
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 140
HpyF3I CTNAG 3 cut(s) 41, 105, 734
Hsp92II CATG 4 cut(s) 199, 232, 668, 682
HspAI GCGC 2 cut(s) 373, 784
Kzo9I GATC 2 cut(s) 400, 616
LmnI GCTCC 4 cut(s) 125, 211, 396, 556
Lsp1109I GCAGC 3 cut(s) 560, 631, 773
LweI GCATC 2 cut(s) 478, 543
MaeI CTAG 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 31, 187
MalI GATC 2 cut(s) 402, 618
MboI GATC 2 cut(s) 400, 616
MboII GAAGA 3 cut(s) 307, 520, 737
MhlI GDGCHC 2 cut(s) 393, 780
MluCI AATT 6 cut(s) 72, 138, 499, 611, 630, 648
MmeI TCCRAC 1 cut(s) 278
MnlI CCTC 6 cut(s) 47, 59, 98, 297, 553, 576
Mph1103I ATGCAT 1 cut(s) 666
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 2 cut(s) 422, 651
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 4 cut(s) 78, 165, 177, 223
MspR9I CCNGG 3 cut(s) 78, 178, 596
MvaI CCWGG 1 cut(s) 596
MwoI GCNNNNNNNGC 2 cut(s) 66, 140
NciI CCSGG 2 cut(s) 78, 178
NdeII GATC 2 cut(s) 400, 616
NlaIII CATG 4 cut(s) 199, 232, 668, 682
NlaIV GGNNCC 3 cut(s) 82, 348, 392
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 31, 187
NsiI ATGCAT 1 cut(s) 666
NspV TTCGAA 1 cut(s) 579
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 2 cut(s) 101, 752
PflFI GACNNNGTC 1 cut(s) 270
PkrI GCNGC 3 cut(s) 550, 646, 788
Psp6I CCWGG 1 cut(s) 594
PspEI GGTNACC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 3 cut(s) 82, 348, 392
PspPI GGNCC 2 cut(s) 225, 347
PstI CTGCAG 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 270
RsaI GTAC 1 cut(s) 541
RsaNI GTAC 1 cut(s) 540
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 2 cut(s) 422, 651
SatI GCNGC 3 cut(s) 549, 645, 787
Sau3AI GATC 2 cut(s) 400, 616
Sau96I GGNCC 2 cut(s) 225, 347
ScrFI CCNGG 3 cut(s) 78, 178, 596
SduI GDGCHC 2 cut(s) 393, 780
SetI ASST 7 cut(s) 58, 111, 208, 462, 522, 545, 553
SfaNI GCATC 2 cut(s) 478, 543
SfcI CTRYAG 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 579
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 6 cut(s) 72, 138, 499, 611, 630, 648
SsiI CCGC 2 cut(s) 343, 607
SspMI CTAG 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 76, 176, 594
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 3 cut(s) 37, 272, 638
TaqI TCGA 5 cut(s) 99, 323, 486, 579, 624
TasI AATT 6 cut(s) 72, 138, 499, 611, 630, 648
TfiI GAWTC 2 cut(s) 101, 752
Tru1I TTAA 2 cut(s) 422, 651
Tru9I TTAA 2 cut(s) 422, 651
TscAI CASTG 2 cut(s) 275, 785
TseFI GTSAC 2 cut(s) 31, 187
TseI GCWGC 3 cut(s) 548, 644, 786
Tsp45I GTSAC 2 cut(s) 31, 187
TspDTI ATGAA 5 cut(s) 249, 353, 492, 695, 699
TspRI CASTG 2 cut(s) 275, 785
Tth111I GACNNNGTC 1 cut(s) 270
XapI RAATTY 2 cut(s) 499, 611
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 1 cut(s) 171
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.