MD02G1168100.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001289
Physical Location & Seq
Reverse (-)
14418377 .. 14425674
7298 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1168100.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGTCGACTGGTTTGACAAAGAGAAGGATGGTGACAGTACTAAGCATTGATGGTGGTGGCATTAGAGGCATAATTCCCAGCACCCTCCTTGCCTTTCTTGAATCCAAGCTTCAGGAATTGGATGGGCCTGCTGCAAGAATCGCAGATTATTTCGACATAATTGCCGGAACAAGCACCGGTGGTCTTGTCACCACCATGCTTACAGCTCCTAACAAAGACAACCGACCCATGTACGAAGCAAAGGACATCAACAACTTCTATTTGGATCACACCCAAAAGATTTTCCCTCAGAATAGTCGTAATAACTTCTTGACATCACTAACAAGTATGGTTGGTGCTGTCATGGGACCAAAATATGATGGAAAGTACCTGCGAACATTGACGAAGGAGCTGCTTGGCGACCTCACTCTTAAACAAACGCTAACCAATGTGATTATTCCAACTTTTGACATTAAGCACCTTCAGCCTGTGCTCTTCTCAACCATCGATGCAAAAGAAAGTCCATTGAAAAATGCTAAGCTTTCGGATATCTGCATCAGTACCTCTGCTGCTCCCACTTTTCTCCCCGCTCATTACTTCGAGGTCAATGATAACGACGGAAGAACCCGAACTTTTGATCTCATCGATGGTGGGGTTGCTGCCAACAATCCTACGATGACGGCCATAAGCCACATAAACAGAGAGATATTGAAGCATAACTCAGAGCCGATGGATGCTACCAGGTTGTTAGTGCTATCATTGGGCACAGGCGCAGCCAAGAATGAAGCGAAGTACAATGCAGCCAACGCCTCCAAATGGGGTTTGATCAATTGGGTCTTTGACAATGGGAGCACACCTTTGGTAGACATTTTCGGCGATGCCAGTTCCGACATGGTTGATTTTCATGTCTCCACCCTCTTCCAATCCGTTCACGCCAAGGACAACTATCTTCGTATTCAGGATGACACGTTGATTGGTGAGGAGGCATCGGTGGACATTGCAACGGAGAAAAATCTGAAAAGGCTTGTTGAAATCGGAAAGGTGTTGTTGAAGAAGCCGGTGTCACGGGTGAATTTGGATACCGGAAGGTATGAGGAATCCAAGGGAGAAGGTACCTATGAAGAAGCACTGATTGAGTTTGCCAAAAGGCTCTCGGAAGGAAAGAAGCTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

385

Amino Acids

42.21

Weight (kDa)

6.03

Isoelectric Point (pI)

33.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 222 2.5e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
Acc65I GGTACC 1 cut(s) 1091
AccB1I GGYRCC 1 cut(s) 1091
AccBSI CCGCTC 1 cut(s) 569
AccI GTMKAC 2 cut(s) 5, 843
AciI CCGC 1 cut(s) 567
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 1 cut(s) 660
AcsI RAATTY 1 cut(s) 1051
AcuI CTGAAG 2 cut(s) 95, 446
AfaI GTAC 6 cut(s) 39, 233, 368, 541, 773, 1093
AfiI CCNNNNNNNGG 1 cut(s) 795
AflIII ACRYGT 1 cut(s) 945
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 5 cut(s) 101, 508, 691, 1010, 1030
AjnI CCWGG 1 cut(s) 719
AluBI AGCT 5 cut(s) 109, 206, 391, 520, 1147
AluI AGCT 5 cut(s) 109, 206, 391, 520, 1147
Alw21I GWGCWC 2 cut(s) 474, 833
Alw26I GTCTC 1 cut(s) 892
AlwI GGATC 1 cut(s) 273
AoxI GGCC 2 cut(s) 125, 660
ApeKI GCWGC 6 cut(s) 131, 391, 548, 638, 752, 779
ApoI RAATTY 1 cut(s) 1051
AsiGI ACCGGT 1 cut(s) 176
Asp718I GGTACC 1 cut(s) 1091
AspLEI GCGC 1 cut(s) 752
AspS9I GGNCC 2 cut(s) 125, 347
AsuHPI GGTGA 4 cut(s) 43, 181, 968, 1060
AvaII GGWCC 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 746
BanI GGYRCC 1 cut(s) 1091
Bbv12I GWGCWC 2 cut(s) 474, 833
BbvI GCAGC 6 cut(s) 118, 378, 535, 625, 764, 791
BccI CCATC 7 cut(s) 22, 44, 116, 353, 491, 620, 703
BceAI ACGGC 1 cut(s) 675
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 373, 407
BciT130I CCWGG 1 cut(s) 721
BciVI GTATCC 1 cut(s) 1051
BclI TGATCA 1 cut(s) 804
BcoDI GTCTC 1 cut(s) 892
BfuAI ACCTGC 1 cut(s) 378
BfuI GTATCC 1 cut(s) 1051
BisI GCNGC 6 cut(s) 132, 392, 549, 639, 753, 780
BlpI GCTNAGC 1 cut(s) 516
BlsI GCNGC 6 cut(s) 133, 393, 550, 640, 754, 781
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 721
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 125, 347
BmiI GGNNCC 2 cut(s) 348, 1093
BmrFI CCNGG 1 cut(s) 721
BmsI GCATC 5 cut(s) 478, 543, 703, 847, 974
Bpu1102I GCTNAGC 1 cut(s) 516
Bsa29I ATCGAT 2 cut(s) 486, 624
BsaJI CCNNGG 2 cut(s) 915, 1080
BsaWI WCCGGW 2 cut(s) 176, 1061
Bsc4I CCNNNNNNNGG 1 cut(s) 795
Bse118I RCCGGY 2 cut(s) 176, 1036
Bse1I ACTGG 2 cut(s) 13, 861
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 1 cut(s) 721
BseCI ATCGAT 2 cut(s) 486, 624
BseDI CCNNGG 2 cut(s) 915, 1080
BseGI GGATG 4 cut(s) 33, 127, 718, 946
BseLI CCNNNNNNNGG 1 cut(s) 795
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 2 cut(s) 302, 714
BseNI ACTGG 2 cut(s) 13, 861
BseRI GAGGAG 1 cut(s) 974
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 6 cut(s) 118, 378, 535, 625, 764, 791
BseYI CCCAGC 1 cut(s) 77
BshFI GGCC 2 cut(s) 127, 662
BshNI GGYRCC 1 cut(s) 1091
BshTI ACCGGT 1 cut(s) 176
BshVI ATCGAT 2 cut(s) 486, 624
BsiHKAI GWGCWC 2 cut(s) 474, 833
BsiSI CCGG 4 cut(s) 165, 177, 1037, 1062
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 795
BsmAI GTCTC 1 cut(s) 892
BsmFI GGGAC 1 cut(s) 360
BsnI GGCC 2 cut(s) 127, 662
Bsp1286I GDGCHC 3 cut(s) 474, 746, 833
Bsp143I GATC 3 cut(s) 265, 616, 804
Bsp1720I GCTNAGC 1 cut(s) 516
BspACI CCGC 1 cut(s) 567
BspANI GGCC 2 cut(s) 127, 662
BspCNI CTCAG 2 cut(s) 301, 713
BspDI ATCGAT 2 cut(s) 486, 624
BspLI GGNNCC 2 cut(s) 348, 1093
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 273
BspQI GCTCTTC 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 1091
BsrBI CCGCTC 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 975
BsrFI RCCGGY 2 cut(s) 176, 1036
BsrI ACTGG 2 cut(s) 13, 861
BssAI RCCGGY 2 cut(s) 176, 1036
BssECI CCNNGG 2 cut(s) 915, 1080
BssMI GATC 3 cut(s) 265, 616, 804
BssT1I CCWWGG 2 cut(s) 915, 1080
Bst2UI CCWGG 1 cut(s) 721
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 2 cut(s) 479, 902
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 4 cut(s) 41, 288, 516, 700
BstF5I GGATG 4 cut(s) 33, 127, 718, 946
BstHHI GCGC 1 cut(s) 752
BstKTI GATC 3 cut(s) 268, 619, 807
BstMAI GTCTC 1 cut(s) 892
BstMBI GATC 3 cut(s) 265, 616, 804
BstMWI GCNNNNNNNGC 4 cut(s) 66, 140, 463, 785
BstNI CCWGG 1 cut(s) 721
BstSCI CCNGG 1 cut(s) 719
BstSLI GKGCMC 1 cut(s) 746
BstV1I GCAGC 6 cut(s) 118, 378, 535, 625, 764, 791
Bsu15I ATCGAT 2 cut(s) 486, 624
BsuI GTATCC 1 cut(s) 1051
BsuRI GGCC 2 cut(s) 127, 662
BsuTUI ATCGAT 2 cut(s) 486, 624
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 4 cut(s) 33, 127, 718, 946
BtsIMutI CAGTG 1 cut(s) 1106
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 129
CfoI GCGC 1 cut(s) 752
Cfr10I RCCGGY 2 cut(s) 176, 1036
Cfr13I GGNCC 2 cut(s) 125, 347
ClaI ATCGAT 2 cut(s) 486, 624
CsiI ACCWGGT 1 cut(s) 719
Csp6I GTAC 6 cut(s) 38, 232, 367, 540, 772, 1092
CspAI ACCGGT 1 cut(s) 176
CviAII CATG 5 cut(s) 196, 229, 343, 871, 884
CviQI GTAC 6 cut(s) 38, 232, 367, 540, 772, 1092
DdeI CTNAG 4 cut(s) 41, 288, 516, 700
DpnI GATC 3 cut(s) 267, 618, 806
DpnII GATC 3 cut(s) 265, 616, 804
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 2 cut(s) 479, 902
EarI CTCTTC 2 cut(s) 479, 902
Eco130I CCWWGG 2 cut(s) 915, 1080
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 347
Eco57I CTGAAG 2 cut(s) 95, 446
EcoRII CCWGG 1 cut(s) 719
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 2 cut(s) 915, 1080
ErhI CCWWGG 2 cut(s) 915, 1080
FaeI CATG 5 cut(s) 199, 232, 346, 874, 887
FaqI GGGAC 1 cut(s) 360
FatI CATG 5 cut(s) 195, 228, 342, 870, 883
FauI CCCGC 1 cut(s) 574
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 2 cut(s) 5, 843
Fnu4HI GCNGC 6 cut(s) 132, 392, 549, 639, 753, 780
FokI GGATG 4 cut(s) 40, 134, 725, 953
Fsp4HI GCNGC 6 cut(s) 132, 392, 549, 639, 753, 780
GlaI GCGC 1 cut(s) 751
GluI GCNGC 6 cut(s) 132, 392, 549, 639, 753, 780
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 2 cut(s) 127, 662
HapII CCGG 4 cut(s) 165, 177, 1037, 1062
HhaI GCGC 1 cut(s) 752
Hin1II CATG 5 cut(s) 199, 232, 346, 874, 887
Hin6I GCGC 1 cut(s) 750
HinP1I GCGC 1 cut(s) 750
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 3 cut(s) 107, 518, 1145
HinfI GANTC 3 cut(s) 101, 138, 1076
HpaII CCGG 4 cut(s) 165, 177, 1037, 1062
HphI GGTGA 4 cut(s) 43, 181, 968, 1060
Hpy166II GTNNAC 4 cut(s) 6, 844, 910, 973
Hpy188I TCNGA 7 cut(s) 291, 526, 703, 868, 996, 1016, 1135
Hpy188III TCNNGA 4 cut(s) 98, 113, 310, 938
Hpy8I GTNNAC 4 cut(s) 6, 844, 910, 973
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 6 cut(s) 18, 379, 470, 1059, 1082, 1130
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 947
HpyCH4V TGCA 5 cut(s) 134, 491, 534, 779, 980
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 140, 463, 785
HpyF3I CTNAG 4 cut(s) 41, 288, 516, 700
HpySE526I ACGT 1 cut(s) 947
Hsp92II CATG 5 cut(s) 199, 232, 346, 874, 887
HspAI GCGC 1 cut(s) 750
KpnI GGTACC 1 cut(s) 1095
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 3 cut(s) 265, 616, 804
LguI GCTCTTC 1 cut(s) 479
LmnI GCTCC 4 cut(s) 211, 388, 556, 828
Lsp1109I GCAGC 6 cut(s) 118, 378, 535, 625, 764, 791
LweI GCATC 5 cut(s) 478, 543, 703, 847, 974
MabI ACCWGGT 1 cut(s) 719
MaeII ACGT 1 cut(s) 947
MaeIII GTNAC 3 cut(s) 31, 187, 1041
MalI GATC 3 cut(s) 267, 618, 806
MbiI CCGCTC 1 cut(s) 569
MboI GATC 3 cut(s) 265, 616, 804
MboII GAAGA 6 cut(s) 466, 612, 889, 920, 1042, 1112
MfeI CAATTG 1 cut(s) 808
MhlI GDGCHC 3 cut(s) 474, 746, 833
MluCI AATT 6 cut(s) 72, 116, 159, 808, 1051, 1150
MmeI TCCRAC 2 cut(s) 464, 891
MseI TTAA 3 cut(s) 411, 453, 1149
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 4 cut(s) 165, 177, 1037, 1062
MspR9I CCNGG 1 cut(s) 721
MunI CAATTG 1 cut(s) 808
MvaI CCWGG 1 cut(s) 721
MwoI GCNNNNNNNGC 4 cut(s) 66, 140, 463, 785
NdeII GATC 3 cut(s) 265, 616, 804
NlaIII CATG 5 cut(s) 199, 232, 346, 874, 887
NlaIV GGNNCC 2 cut(s) 348, 1093
NmuCI GTSAC 3 cut(s) 31, 187, 1041
PciSI GCTCTTC 1 cut(s) 479
PfeI GAWTC 3 cut(s) 101, 138, 1076
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 6 cut(s) 133, 393, 550, 640, 754, 781
Psp6I CCWGG 1 cut(s) 719
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 1 cut(s) 719
PspN4I GGNNCC 2 cut(s) 348, 1093
PspPI GGNCC 2 cut(s) 125, 347
RsaI GTAC 6 cut(s) 39, 233, 368, 541, 773, 1093
RsaNI GTAC 6 cut(s) 38, 232, 367, 540, 772, 1092
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 479
SaqAI TTAA 3 cut(s) 411, 453, 1149
SatI GCNGC 6 cut(s) 132, 392, 549, 639, 753, 780
Sau3AI GATC 3 cut(s) 265, 616, 804
Sau96I GGNCC 2 cut(s) 125, 347
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 721
SduI GDGCHC 3 cut(s) 474, 746, 833
SexAI ACCWGGT 1 cut(s) 719
SfaNI GCATC 5 cut(s) 478, 543, 703, 847, 974
SgrAI CRCCGGYG 1 cut(s) 176
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 6 cut(s) 72, 116, 159, 808, 1051, 1150
SsiI CCGC 1 cut(s) 567
StyD4I CCNGG 1 cut(s) 719
StyI CCWWGG 2 cut(s) 915, 1080
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 1 cut(s) 950
TaqI TCGA 5 cut(s) 5, 153, 486, 579, 624
TasI AATT 6 cut(s) 72, 116, 159, 808, 1051, 1150
TatI WGTACW 2 cut(s) 37, 771
TfiI GAWTC 3 cut(s) 101, 138, 1076
Tru1I TTAA 3 cut(s) 411, 453, 1149
Tru9I TTAA 3 cut(s) 411, 453, 1149
TscAI CASTG 1 cut(s) 1113
TseFI GTSAC 3 cut(s) 31, 187, 1041
TseI GCWGC 6 cut(s) 131, 391, 548, 638, 752, 779
Tsp45I GTSAC 3 cut(s) 31, 187, 1041
TspDTI ATGAA 3 cut(s) 777, 872, 1113
TspGWI ACGGA 3 cut(s) 612, 895, 998
TspRI CASTG 1 cut(s) 1113
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 1 cut(s) 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XmiI GTMKAC 2 cut(s) 5, 843
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.