MD12G1245800.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001289
Physical Location & Seq
Forward (+)
31611294 .. 31611666
373 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1245800.v1.1.491

Sequence Viewer

Length: 243 bp
ATGCTCGCCTTTCTCGAGTCCAAACTCCAGAAACTGGATGGGAAAGATGCGAGGATTGCAGACTACTTTGACTACATTGCTGGAACCAGCACAGGAGGCCTTGTTACTGCTATGATTACCACACCAAATGAAAAAAAGCAACCTCTGTTTGAAGCACATGAACTCAAGAAGTTTTATTTTGATGAGAGTCCATACATATTCCCTCAAGAACCGACCAAAACCGCTCGAATGTACGTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

81

Amino Acids

9.15

Weight (kDa)

5.73

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 12 - 64 2.6e-06 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 34
AccBSI CCGCTC 1 cut(s) 224
AciI CCGC 1 cut(s) 222
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 1 cut(s) 152
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 14
BccI CCATC 1 cut(s) 32
BmeT110I CYCGRG 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 85
BmsI GCATC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 11
BpuEI CTTGAG 2 cut(s) 149, 189
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 75
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMI GCAATG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 39
BshFI GGCC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 14
BslI CCNNNNNNNGG 1 cut(s) 34
BsnI GGCC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 14
BspACI CCGC 1 cut(s) 222
BspANI GGCC 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 85
BsrBI CCGCTC 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 75
BsrI ACTGG 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 6
BstF5I GGATG 1 cut(s) 43
BstMWI GCNNNNNNNGC 2 cut(s) 56, 96
BsuRI GGCC 1 cut(s) 99
BtsCI GGATG 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 34
Csp6I GTAC 1 cut(s) 232
CviAII CATG 1 cut(s) 158
CviJI RGCY 1 cut(s) 99
CviKI_1 RGCY 1 cut(s) 99
CviQI GTAC 1 cut(s) 232
Eco147I AGGCCT 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 14
FaeI CATG 1 cut(s) 161
FaiI YATR 5 cut(s) 113, 159, 193, 197, 241
FatI CATG 1 cut(s) 157
FokI GGATG 1 cut(s) 50
GsuI CTGGAG 1 cut(s) 11
HaeIII GGCC 1 cut(s) 99
Hin1II CATG 1 cut(s) 161
HinfI GANTC 2 cut(s) 17, 187
Hpy188III TCNNGA 4 cut(s) 14, 28, 166, 206
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 96
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 1 cut(s) 161
LpnPI CCDG 5 cut(s) 20, 41, 66, 78, 100
LweI GCATC 1 cut(s) 37
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 103
MbiI CCGCTC 1 cut(s) 224
MlyI GAGTC 2 cut(s) 26, 196
MnlI CCTC 4 cut(s) 45, 89, 153, 213
MwoI GCNNNNNNNGC 2 cut(s) 56, 96
NlaIII CATG 1 cut(s) 161
NlaIV GGNNCC 1 cut(s) 85
PaeR7I CTCGAG 1 cut(s) 14
PceI AGGCCT 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PflMI CCANNNNNTGG 1 cut(s) 34
PleI GAGTC 2 cut(s) 25, 195
PpsI GAGTC 2 cut(s) 25, 195
PspN4I GGNNCC 1 cut(s) 85
PstNI CAGNNNCTG 1 cut(s) 34
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
SchI GAGTC 2 cut(s) 26, 196
SetI ASST 2 cut(s) 145, 237
SfaNI GCATC 1 cut(s) 37
Sfr274I CTCGAG 1 cut(s) 14
SlaI CTCGAG 1 cut(s) 14
SmlI CTYRAG 3 cut(s) 14, 164, 204
SmoI CTYRAG 3 cut(s) 14, 164, 204
SseBI AGGCCT 1 cut(s) 99
SsiI CCGC 1 cut(s) 222
StuI AGGCCT 1 cut(s) 99
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 15, 226
TspDTI ATGAA 2 cut(s) 144, 174
Van91I CCANNNNNTGG 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.