Rroxscaffold_2G00138680

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001289
Physical Location & Seq
Forward (+)
76853297 .. 76855420
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138680.1

Sequence Viewer

Length: 1158 bp
ATGTCGATTGGTTTGGCAAAGAGAAAGATGGTGACAGTTCTGAGCATTGATGGAGGGGGCATTAGAGGCATAATTCCAAGCACCATCCTCGGCTTTCTCGAATCTAAGCTTCAGGATTTGGATGGAGCAAATGCAAGAATTGCAGATTACTTTGACATTATCGCTGGAACTAGCACCGGTGGGTTGGTCACCACCATGCTTACAGCTCCCAACAAGAACAATCGACCCATGTATGAAGCCAAGGACATCAACAACTTTTATTTAGAACACTGTCCCCATATTTTCCCTCAAAAAAGTCGGAAAAACTTCTTCACATCGATCACAAATGCAATGAAACCGGGGCCAAAATACGATGGGAAGTATCTGCGCTCATTGACAAACGGCCTGCTTGGTGACCTCACTCTCAAGCAGACTTTAACCAATGTGGTCATTCCAACTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCACAACCATCGACGCAAAAGAAAATTCATTGAAGAATGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATCTTATCTCCCCCCACACTACTTTGAAGTCAAGGATGACCAGGGAAATATCCGCACGTTTGATCTTGTCGATGGTGGGGTTGCTGCAAACAACCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGAACAACTCAGAACCGATGGATGCGACTCGGTTGCAAGTGCTGTCATTGGGCACCGGTGCTGCCAAGTATGCAGAGAAGTACAGTGCAGTCGAGGCATCAAAATGGGGTTTGGTGAATTGGATGTTTGATAATGGGAGCACACCTTTGCTGGACATTTTTGGCGATGCAAGTTCTGATATGGTTGACTTTCATGTCTCCACTCTCTTCCAGTCCCTCAATGTCAAGGACAATTACCTAAGAATTCAGGATGACACATTGAGCGGTGAAGAATCATCAGTGGATATTGCAACCGAGAAGAATCTGGAAAGCCTTGTGGTGATCGGAAACGGGTTGCTAAAGAAGCCAGTGTCAAGGGTGAATATGGATACAGGGAGGTATGAGAAATGTGAGGGAGAAGCCACCTATGAAGAAGCACTAGTCCATTTTGCCATACAGCTCTCGGAGGGAAGGAAGCTCAGACAAACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

385

Amino Acids

42.38

Weight (kDa)

5.86

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 220 1.9e-28 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 878
AccB1I GGYRCC 1 cut(s) 737
AccBSI CCGCTC 1 cut(s) 948
AciI CCGC 2 cut(s) 601, 948
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 2 cut(s) 493, 927
AcuI CTGAAG 2 cut(s) 95, 440
AfaI GTAC 2 cut(s) 535, 767
AgeI ACCGGT 2 cut(s) 176, 740
AgsI TTSAA 3 cut(s) 502, 575, 685
AhlI ACTAGT 1 cut(s) 1102
AjnI CCWGG 1 cut(s) 588
AluBI AGCT 6 cut(s) 109, 206, 514, 545, 1123, 1141
AluI AGCT 6 cut(s) 109, 206, 514, 545, 1123, 1141
Alw21I GWGCWC 1 cut(s) 827
Alw26I GTCTC 1 cut(s) 886
AoxI GGCC 3 cut(s) 341, 382, 654
ApeKI GCWGC 3 cut(s) 542, 632, 746
ApoI RAATTY 2 cut(s) 493, 927
AsiGI ACCGGT 2 cut(s) 176, 740
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 341
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 7 cut(s) 43, 181, 404, 811, 962, 1015, 1054
BaeGI GKGCMC 1 cut(s) 740
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 827
BbvI GCAGC 3 cut(s) 554, 619, 733
BceAI ACGGC 1 cut(s) 397
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 700, 734
BciT130I CCWGG 1 cut(s) 590
BciVI GTATCC 1 cut(s) 1045
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 886
BcuI ACTAGT 1 cut(s) 1102
BfaI CTAG 2 cut(s) 171, 1103
BfmI CTRYAG 1 cut(s) 540
BfuI GTATCC 1 cut(s) 1045
BisI GCNGC 3 cut(s) 543, 633, 747
BlsI GCNGC 3 cut(s) 544, 634, 748
Bme1390I CCNGG 2 cut(s) 339, 590
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 2 cut(s) 342, 739
BmrFI CCNGG 2 cut(s) 339, 590
BmsI GCATC 4 cut(s) 537, 697, 791, 841
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 339
Bsa29I ATCGAT 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 88, 240, 338, 589
BsaWI WCCGGW 2 cut(s) 176, 740
Bse118I RCCGGY 2 cut(s) 176, 740
Bse1I ACTGG 2 cut(s) 895, 1031
Bse3DI GCAATG 1 cut(s) 336
BseBI CCWGG 1 cut(s) 590
BseCI ATCGAT 1 cut(s) 317
BseDI CCNNGG 4 cut(s) 88, 240, 338, 589
BseGI GGATG 6 cut(s) 84, 127, 589, 712, 813, 940
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 3 cut(s) 32, 708, 1156
BseNI ACTGG 2 cut(s) 895, 1031
BseSI GKGCMC 1 cut(s) 740
BseXI GCAGC 3 cut(s) 554, 619, 733
BsgI GTGCAG 1 cut(s) 792
BshFI GGCC 3 cut(s) 343, 384, 656
BshNI GGYRCC 1 cut(s) 737
BshTI ACCGGT 2 cut(s) 176, 740
BshVI ATCGAT 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 827
BsiSI CCGG 3 cut(s) 177, 338, 741
BslFI GGGAC 2 cut(s) 258, 883
BsmAI GTCTC 1 cut(s) 886
BsmFI GGGAC 2 cut(s) 258, 883
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 3 cut(s) 343, 384, 656
Bsp1286I GDGCHC 2 cut(s) 740, 827
Bsp143I GATC 3 cut(s) 318, 610, 1005
BspACI CCGC 2 cut(s) 601, 948
BspANI GGCC 3 cut(s) 343, 384, 656
BspCNI CTCAG 3 cut(s) 33, 707, 1155
BspDI ATCGAT 1 cut(s) 317
BspLI GGNNCC 2 cut(s) 342, 739
BspMAI CTGCAG 1 cut(s) 544
BspT107I GGYRCC 1 cut(s) 737
BsrBI CCGCTC 1 cut(s) 948
BsrDI GCAATG 1 cut(s) 336
BsrFI RCCGGY 2 cut(s) 176, 740
BsrI ACTGG 2 cut(s) 895, 1031
BssAI RCCGGY 2 cut(s) 176, 740
BssECI CCNNGG 4 cut(s) 88, 240, 338, 589
BssMI GATC 3 cut(s) 318, 610, 1005
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 3 cut(s) 37, 272, 770
Bst6I CTCTTC 1 cut(s) 896
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 386
BstDEI CTNAG 5 cut(s) 41, 105, 694, 923, 1142
BstEII GGTNACC 2 cut(s) 187, 392
BstF5I GGATG 6 cut(s) 84, 127, 589, 712, 813, 940
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 3 cut(s) 321, 613, 1008
BstMAI GTCTC 1 cut(s) 886
BstMBI GATC 3 cut(s) 318, 610, 1005
BstMWI GCNNNNNNNGC 5 cut(s) 66, 140, 755, 779, 1027
BstNI CCWGG 1 cut(s) 590
BstPI GGTNACC 2 cut(s) 187, 392
BstSCI CCNGG 2 cut(s) 337, 588
BstSFI CTRYAG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 740
BstV1I GCAGC 3 cut(s) 554, 619, 733
Bsu15I ATCGAT 1 cut(s) 317
BsuI GTATCC 1 cut(s) 1045
BsuRI GGCC 3 cut(s) 343, 384, 656
BsuTUI ATCGAT 1 cut(s) 317
BtgZI GCGATG 1 cut(s) 864
BtsCI GGATG 6 cut(s) 84, 127, 589, 712, 813, 940
BtsIMutI CAGTG 4 cut(s) 268, 775, 969, 1038
Cac8I GCNNGC 1 cut(s) 386
CfoI GCGC 1 cut(s) 369
Cfr10I RCCGGY 2 cut(s) 176, 740
Cfr13I GGNCC 1 cut(s) 341
ClaI ATCGAT 1 cut(s) 317
CseI GACGC 1 cut(s) 491
Csp6I GTAC 2 cut(s) 534, 766
CspAI ACCGGT 2 cut(s) 176, 740
CviAII CATG 3 cut(s) 196, 229, 878
CviQI GTAC 2 cut(s) 534, 766
DdeI CTNAG 5 cut(s) 41, 105, 694, 923, 1142
DpnI GATC 3 cut(s) 320, 612, 1007
DpnII GATC 3 cut(s) 318, 610, 1005
DrdI GACNNNNNNGTC 1 cut(s) 878
DseDI GACNNNNNNGTC 1 cut(s) 878
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 896
EarI CTCTTC 1 cut(s) 896
Eco130I CCWWGG 1 cut(s) 240
Eco32I GATATC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 95, 440
Eco91I GGTNACC 2 cut(s) 187, 392
EcoO65I GGTNACC 2 cut(s) 187, 392
EcoRI GAATTC 1 cut(s) 927
EcoRII CCWGG 1 cut(s) 588
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 3 cut(s) 199, 232, 881
FaqI GGGAC 2 cut(s) 258, 883
FatI CATG 3 cut(s) 195, 228, 877
Fnu4HI GCNGC 3 cut(s) 543, 633, 747
FokI GGATG 6 cut(s) 71, 134, 596, 719, 820, 947
Fsp4HI GCNGC 3 cut(s) 543, 633, 747
FspBI CTAG 2 cut(s) 171, 1103
GlaI GCGC 1 cut(s) 368
GluI GCNGC 3 cut(s) 543, 633, 747
HaeIII GGCC 3 cut(s) 343, 384, 656
HapII CCGG 3 cut(s) 177, 338, 741
HgaI GACGC 1 cut(s) 491
HhaI GCGC 1 cut(s) 369
Hin1II CATG 3 cut(s) 199, 232, 881
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 2 cut(s) 107, 512
HinfI GANTC 4 cut(s) 101, 712, 956, 985
HpaII CCGG 3 cut(s) 177, 338, 741
HphI GGTGA 7 cut(s) 43, 181, 404, 811, 962, 1015, 1054
Hpy166II GTNNAC 1 cut(s) 871
Hpy188I TCNGA 8 cut(s) 42, 300, 520, 697, 862, 1010, 1129, 1145
Hpy188III TCNNGA 4 cut(s) 98, 113, 932, 989
Hpy8I GTNNAC 1 cut(s) 871
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 2 cut(s) 464, 1128
HpyCH4III ACNGT 3 cut(s) 37, 272, 770
HpyCH4IV ACGT 1 cut(s) 605
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 140, 755, 779, 1027
HpyF3I CTNAG 5 cut(s) 41, 105, 694, 923, 1142
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 3 cut(s) 199, 232, 881
HspAI GCGC 1 cut(s) 367
Kzo9I GATC 3 cut(s) 318, 610, 1005
LmnI GCTCC 4 cut(s) 125, 211, 550, 822
Lsp1109I GCAGC 3 cut(s) 554, 619, 733
LweI GCATC 4 cut(s) 537, 697, 791, 841
MaeI CTAG 2 cut(s) 171, 1103
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 3 cut(s) 31, 187, 392
MalI GATC 3 cut(s) 320, 612, 1007
MbiI CCGCTC 1 cut(s) 948
MboI GATC 3 cut(s) 318, 610, 1005
MboII GAAGA 7 cut(s) 301, 514, 697, 883, 965, 994, 1106
MfeI CAATTG 1 cut(s) 1153
MhlI GDGCHC 2 cut(s) 740, 827
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 7 cut(s) 72, 138, 493, 802, 916, 927, 1153
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 1 cut(s) 706
MmeI TCCRAC 2 cut(s) 278, 458
Mox20I TGGCCA 1 cut(s) 656
MroXI GAANNNNTTC 1 cut(s) 305
MscI TGGCCA 1 cut(s) 656
MseI TTAA 1 cut(s) 416
MslI CAYNNNNRTG 2 cut(s) 194, 787
Msp20I TGGCCA 1 cut(s) 656
MspI CCGG 3 cut(s) 177, 338, 741
MspR9I CCNGG 2 cut(s) 339, 590
MunI CAATTG 1 cut(s) 1153
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 1 cut(s) 590
MwoI GCNNNNNNNGC 5 cut(s) 66, 140, 755, 779, 1027
NciI CCSGG 1 cut(s) 339
NdeII GATC 3 cut(s) 318, 610, 1005
NlaIII CATG 3 cut(s) 199, 232, 881
NlaIV GGNNCC 2 cut(s) 342, 739
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 3 cut(s) 31, 187, 392
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 3 cut(s) 101, 956, 985
PinAI ACCGGT 2 cut(s) 176, 740
PkrI GCNGC 3 cut(s) 544, 634, 748
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
Psp6I CCWGG 1 cut(s) 588
PspEI GGTNACC 2 cut(s) 187, 392
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 2 cut(s) 342, 739
PspPI GGNCC 1 cut(s) 341
PstI CTGCAG 1 cut(s) 544
RsaI GTAC 2 cut(s) 535, 767
RsaNI GTAC 2 cut(s) 534, 766
RseI CAYNNNNRTG 2 cut(s) 194, 787
SaqAI TTAA 1 cut(s) 416
SatI GCNGC 3 cut(s) 543, 633, 747
Sau3AI GATC 3 cut(s) 318, 610, 1005
Sau96I GGNCC 1 cut(s) 341
SchI GAGTC 1 cut(s) 706
ScrFI CCNGG 2 cut(s) 339, 590
SduI GDGCHC 2 cut(s) 740, 827
SfaNI GCATC 4 cut(s) 537, 697, 791, 841
SfcI CTRYAG 1 cut(s) 540
SgrAI CRCCGGYG 2 cut(s) 176, 740
SmiMI CAYNNNNRTG 2 cut(s) 194, 787
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SpeI ACTAGT 1 cut(s) 1102
Sse9I AATT 7 cut(s) 72, 138, 493, 802, 916, 927, 1153
SsiI CCGC 2 cut(s) 601, 948
SspMI CTAG 2 cut(s) 171, 1103
StyD4I CCNGG 2 cut(s) 337, 588
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 3 cut(s) 37, 272, 770
TaiI ACGT 1 cut(s) 608
TaqI TCGA 7 cut(s) 5, 99, 223, 317, 480, 618, 777
TasI AATT 7 cut(s) 72, 138, 493, 802, 916, 927, 1153
TatI WGTACW 1 cut(s) 765
TfiI GAWTC 3 cut(s) 101, 956, 985
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TscAI CASTG 4 cut(s) 275, 775, 969, 1038
TseFI GTSAC 3 cut(s) 31, 187, 392
TseI GCWGC 3 cut(s) 542, 632, 746
Tsp45I GTSAC 3 cut(s) 31, 187, 392
TspDTI ATGAA 5 cut(s) 249, 347, 486, 866, 1107
TspRI CASTG 4 cut(s) 275, 775, 969, 1038
XapI RAATTY 2 cut(s) 493, 927
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 2 cut(s) 171, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.