Rh2DG181100

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001289
Physical Location & Seq
Reverse (-)
15953544 .. 15955832
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG181100.1

Sequence Viewer

Length: 1164 bp
ATGTCTACTGGTTTGGCAAAGAGAAAGATGGTGACAGTGTTAAGCATTGACGGAGGTGGTATTAGAGGCATCATCCCCGGCACTATCCTCGGCTTTCTCGAATCCAAGCTTCAGGATTTGGATGGCCCGAGTGCAAGACTTGCAGATTATTTTGATATAATCGCCGGAACTAGCACAGGTGGATTGGTCACCACCATGCTTACAGCTCCCAACAAGGACAATCGACCCATGTATGAAGCAAAGGACATCAATAACTTCTACTTGGAACAAACACCCAAGATTTTCCCTCAGAAGAGTCGAAAGAACTTCTTGACATCAATTCAAAGTTTGGTTGGAGCGGTGACCGGACCAAAATACGACGGGAATTACCTGCGCACATTGACGACCGGGCTGCTTGGCGACCTCACAATAAAAGAAACTCTAACCAATGTCATCATCCCAACGTTCGATATCAAACACCTTCAGCCTGTGATTTTCTCAACCATTGATGCAAAAGAAAGTTCTTTGAAAAATGCAAAGCTTTCAGATATCTGCATCAGTACCTCTGCAGCTCCAACTTTTCTCCCCGCACACTACTTTGAGGTTACGGACAAACAGGGAACAACTCGTTCTTTTGATCTCGTTGATGGTGGGGTCGCTGCAAATAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGCACGACTCAGAGCCATTGGATGCCACTCGGTTACTAGTGCTCTCATTGGGCACCGGCTCCGCCAAGTCTGAAGGGAAGTACACCGCAGCCGAGGCCTCAAAATGGGGTTTGATCAATTGGTTGTTTAACAATGGGAGCACACCTTTGGTTGACATGTTTGGCGATGCGAGTGCTGATATGGTTGACATTCATGTCTCCACTATGTTCCAGTCCCTCAATTGCAACGATAACTACATACGCATTCAGGATGATAATTTGATCGGTGAGGAGGCATCAGTGGACATTGCAAGAGAGAAGAATCTGAAGAGGCTTGTGGAGATCGGAAATGCGCTGTTGAAGAAGCGATTGTCAAGGGTAAATCTCGATACCGGGAGGTACGAGGAATCTGAGGGAGAAGGTACCTACGAAGAAGCACTGGTCGATTTTGCCAAACGGCTCGCGGAGGGAAGGAAGCTCAGGCAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

387

Amino Acids

42.41

Weight (kDa)

5.86

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 222 1.2e-24 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 884
Acc16I TGCGCA 1 cut(s) 374
Acc36I ACCTGC 1 cut(s) 378
Acc65I GGTACC 1 cut(s) 1091
AccB1I GGYRCC 2 cut(s) 743, 1091
AccBSI CCGCTC 1 cut(s) 338
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 1133
AciI CCGC 5 cut(s) 338, 567, 753, 777, 1133
AclI AACGTT 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 660
AcuI CTGAAG 4 cut(s) 95, 446, 783, 1016
AfaI GTAC 4 cut(s) 541, 773, 1070, 1093
AfiI CCNNNNNNNGG 1 cut(s) 795
AflIII ACRYGT 1 cut(s) 846
AgsI TTSAA 4 cut(s) 323, 508, 691, 1030
AhlI ACTAGT 1 cut(s) 727
AluBI AGCT 5 cut(s) 109, 206, 520, 551, 1147
AluI AGCT 5 cut(s) 109, 206, 520, 551, 1147
Alw21I GWGCWC 2 cut(s) 735, 833
Alw26I GTCTC 1 cut(s) 892
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 3 cut(s) 124, 660, 786
ApeKI GCWGC 4 cut(s) 391, 548, 638, 779
Asp718I GGTACC 1 cut(s) 1091
AspLEI GCGC 2 cut(s) 375, 1024
AspS9I GGNCC 2 cut(s) 125, 347
AsuC2I CCSGG 3 cut(s) 78, 388, 1063
AsuHPI GGTGA 4 cut(s) 43, 181, 352, 968
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 746
BalI TGGCCA 1 cut(s) 662
BanI GGYRCC 2 cut(s) 743, 1091
Bbv12I GWGCWC 2 cut(s) 735, 833
BbvI GCAGC 4 cut(s) 378, 560, 625, 791
BccI CCATC 4 cut(s) 22, 116, 620, 652
BceAI ACGGC 1 cut(s) 1142
BcgI CGANNNNNNTGC 6 cut(s) 70, 104, 373, 407, 845, 879
BclI TGATCA 1 cut(s) 804
BcnI CCSGG 3 cut(s) 78, 388, 1063
BcoDI GTCTC 1 cut(s) 892
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 2 cut(s) 171, 728
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 378
BisI GCNGC 4 cut(s) 392, 549, 639, 780
BlsI GCNGC 4 cut(s) 393, 550, 640, 781
Bme1390I CCNGG 3 cut(s) 78, 388, 1063
Bme18I GGWCC 1 cut(s) 347
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 125, 347
BmiI GGNNCC 3 cut(s) 745, 751, 1093
BmrFI CCNGG 3 cut(s) 78, 388, 1063
BmsI GCATC 6 cut(s) 78, 478, 543, 703, 847, 974
Bpu10I CCTNAGC 1 cut(s) 1148
BpuMI CCSGG 3 cut(s) 78, 388, 1063
BsaJI CCNNGG 3 cut(s) 76, 88, 783
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 795
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 3 cut(s) 13, 901, 1113
Bse3DI GCAATG 1 cut(s) 975
BseDI CCNNGG 3 cut(s) 76, 88, 783
BseGI GGATG 5 cut(s) 72, 127, 435, 718, 946
BseLI CCNNNNNNNGG 1 cut(s) 795
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 4 cut(s) 302, 714, 1071, 1162
BseNI ACTGG 3 cut(s) 13, 901, 1113
BseRI GAGGAG 1 cut(s) 974
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 4 cut(s) 378, 560, 625, 791
Bsh1236I CGCG 1 cut(s) 1133
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 3 cut(s) 126, 662, 788
BshNI GGYRCC 2 cut(s) 743, 1091
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 2 cut(s) 735, 833
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 6 cut(s) 78, 165, 345, 387, 747, 1062
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 1 cut(s) 795
BsmAI GTCTC 1 cut(s) 892
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 3 cut(s) 126, 662, 788
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 3 cut(s) 735, 746, 833
Bsp143I GATC 4 cut(s) 616, 804, 951, 1011
BspACI CCGC 5 cut(s) 338, 567, 753, 777, 1133
BspANI GGCC 3 cut(s) 126, 662, 788
BspCNI CTCAG 4 cut(s) 301, 713, 1072, 1161
BspFNI CGCG 1 cut(s) 1133
BspLI GGNNCC 3 cut(s) 745, 751, 1093
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 378
BspT107I GGYRCC 2 cut(s) 743, 1091
BsrBI CCGCTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 975
BsrFI RCCGGY 1 cut(s) 746
BsrI ACTGG 3 cut(s) 13, 901, 1113
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 3 cut(s) 76, 88, 783
BssMI GATC 4 cut(s) 616, 804, 951, 1011
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 2 cut(s) 287, 992
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 1131
BstDEI CTNAG 4 cut(s) 288, 700, 1080, 1148
BstEII GGTNACC 2 cut(s) 187, 340
BstF5I GGATG 5 cut(s) 72, 127, 435, 718, 946
BstFNI CGCG 1 cut(s) 1133
BstHHI GCGC 2 cut(s) 375, 1024
BstKTI GATC 4 cut(s) 619, 807, 954, 1014
BstMAI GTCTC 1 cut(s) 892
BstMBI GATC 4 cut(s) 616, 804, 951, 1011
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 2 cut(s) 140, 785
BstNSI RCATGY 1 cut(s) 850
BstPI GGTNACC 2 cut(s) 187, 340
BstSCI CCNGG 3 cut(s) 76, 386, 1061
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 746
BstUI CGCG 1 cut(s) 1133
BstV1I GCAGC 4 cut(s) 378, 560, 625, 791
BsuRI GGCC 3 cut(s) 126, 662, 788
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 5 cut(s) 72, 127, 435, 718, 946
BtsIMutI CAGTG 3 cut(s) 42, 975, 1106
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 1131
CfoI GCGC 2 cut(s) 375, 1024
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 2 cut(s) 125, 347
Csp6I GTAC 4 cut(s) 540, 772, 1069, 1092
CviAII CATG 4 cut(s) 196, 229, 847, 884
CviQI GTAC 4 cut(s) 540, 772, 1069, 1092
DdeI CTNAG 4 cut(s) 288, 700, 1080, 1148
DpnI GATC 4 cut(s) 618, 806, 953, 1013
DpnII GATC 4 cut(s) 616, 804, 951, 1011
DrdI GACNNNNNNGTC 1 cut(s) 884
DseDI GACNNNNNNGTC 1 cut(s) 884
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 2 cut(s) 287, 992
EarI CTCTTC 2 cut(s) 287, 992
EciI GGCGGA 1 cut(s) 742
Eco147I AGGCCT 1 cut(s) 788
Eco32I GATATC 2 cut(s) 451, 529
Eco47I GGWCC 1 cut(s) 347
Eco57I CTGAAG 4 cut(s) 95, 446, 783, 1016
Eco88I CYCGRG 1 cut(s) 127
Eco91I GGTNACC 2 cut(s) 187, 340
EcoO65I GGTNACC 2 cut(s) 187, 340
EcoRV GATATC 2 cut(s) 451, 529
FaeI CATG 4 cut(s) 199, 232, 850, 887
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 1 cut(s) 889
FatI CATG 4 cut(s) 195, 228, 846, 883
FauI CCCGC 1 cut(s) 574
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 392, 549, 639, 780
FokI GGATG 5 cut(s) 59, 134, 422, 725, 953
Fsp4HI GCNGC 4 cut(s) 392, 549, 639, 780
FspBI CTAG 2 cut(s) 171, 728
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 2 cut(s) 374, 1023
GluI GCNGC 4 cut(s) 392, 549, 639, 780
HaeIII GGCC 3 cut(s) 126, 662, 788
HapII CCGG 6 cut(s) 78, 165, 345, 387, 747, 1062
HhaI GCGC 2 cut(s) 375, 1024
Hin1II CATG 4 cut(s) 199, 232, 850, 887
Hin6I GCGC 2 cut(s) 373, 1022
HinP1I GCGC 2 cut(s) 373, 1022
HincII GTYRAC 2 cut(s) 844, 877
HindII GTYRAC 2 cut(s) 844, 877
HindIII AAGCTT 2 cut(s) 107, 518
HinfI GANTC 5 cut(s) 101, 295, 698, 991, 1076
HpaII CCGG 6 cut(s) 78, 165, 345, 387, 747, 1062
HphI GGTGA 4 cut(s) 43, 181, 352, 968
Hpy166II GTNNAC 5 cut(s) 6, 774, 844, 877, 973
Hpy188I TCNGA 7 cut(s) 291, 526, 703, 763, 996, 1016, 1081
Hpy188III TCNNGA 5 cut(s) 98, 113, 310, 938, 1055
Hpy8I GTNNAC 5 cut(s) 6, 774, 844, 877, 973
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 4 cut(s) 470, 758, 1082, 1134
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 9 cut(s) 134, 143, 491, 515, 534, 548, 641, 915, 980
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 785
HpyF3I CTNAG 4 cut(s) 288, 700, 1080, 1148
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 4 cut(s) 199, 232, 850, 887
HspAI GCGC 2 cut(s) 373, 1022
KpnI GGTACC 1 cut(s) 1095
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 4 cut(s) 616, 804, 951, 1011
LmnI GCTCC 5 cut(s) 211, 335, 556, 755, 828
Lsp1109I GCAGC 4 cut(s) 378, 560, 625, 791
LweI GCATC 6 cut(s) 78, 478, 543, 703, 847, 974
MaeI CTAG 2 cut(s) 171, 728
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 5 cut(s) 31, 187, 340, 583, 723
MalI GATC 4 cut(s) 618, 806, 953, 1013
MbiI CCGCTC 1 cut(s) 338
MboI GATC 4 cut(s) 616, 804, 951, 1011
MboII GAAGA 5 cut(s) 304, 1000, 1009, 1042, 1112
MfeI CAATTG 2 cut(s) 808, 910
MhlI GDGCHC 3 cut(s) 735, 746, 833
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 6 cut(s) 318, 364, 808, 910, 946, 1159
MluNI TGGCCA 1 cut(s) 662
MlyI GAGTC 2 cut(s) 304, 692
MmeI TCCRAC 2 cut(s) 313, 578
Mox20I TGGCCA 1 cut(s) 662
MscI TGGCCA 1 cut(s) 662
MseI TTAA 2 cut(s) 41, 819
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 662
MspI CCGG 6 cut(s) 78, 165, 345, 387, 747, 1062
MspR9I CCNGG 3 cut(s) 78, 388, 1063
MunI CAATTG 2 cut(s) 808, 910
Mva1269I GAATGC 1 cut(s) 933
MvnI CGCG 1 cut(s) 1133
MwoI GCNNNNNNNGC 2 cut(s) 140, 785
NciI CCSGG 3 cut(s) 78, 388, 1063
NdeII GATC 4 cut(s) 616, 804, 951, 1011
NlaIII CATG 4 cut(s) 199, 232, 850, 887
NlaIV GGNNCC 3 cut(s) 745, 751, 1093
NmeAIII GCCGAG 2 cut(s) 69, 808
NmuCI GTSAC 3 cut(s) 31, 187, 340
NsbI TGCGCA 1 cut(s) 374
NspI RCATGY 1 cut(s) 850
PceI AGGCCT 1 cut(s) 788
PciI ACATGT 1 cut(s) 846
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 933
PfeI GAWTC 3 cut(s) 101, 991, 1076
PkrI GCNGC 4 cut(s) 393, 550, 640, 781
PleI GAGTC 2 cut(s) 303, 692
PpsI GAGTC 2 cut(s) 303, 692
PscI ACATGT 1 cut(s) 846
Psp1406I AACGTT 1 cut(s) 443
PspEI GGTNACC 2 cut(s) 187, 340
PspN4I GGNNCC 3 cut(s) 745, 751, 1093
PspPI GGNCC 2 cut(s) 125, 347
PstI CTGCAG 1 cut(s) 550
RsaI GTAC 4 cut(s) 541, 773, 1070, 1093
RsaNI GTAC 4 cut(s) 540, 772, 1069, 1092
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 2 cut(s) 41, 819
SatI GCNGC 4 cut(s) 392, 549, 639, 780
Sau3AI GATC 4 cut(s) 616, 804, 951, 1011
Sau96I GGNCC 2 cut(s) 125, 347
SchI GAGTC 2 cut(s) 304, 692
ScrFI CCNGG 3 cut(s) 78, 388, 1063
SduI GDGCHC 3 cut(s) 735, 746, 833
SfaNI GCATC 6 cut(s) 78, 478, 543, 703, 847, 974
SfcI CTRYAG 1 cut(s) 546
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 194
SpeI ACTAGT 1 cut(s) 727
Sse9I AATT 6 cut(s) 318, 364, 808, 910, 946, 1159
SseBI AGGCCT 1 cut(s) 788
SsiI CCGC 5 cut(s) 338, 567, 753, 777, 1133
SspMI CTAG 2 cut(s) 171, 728
StuI AGGCCT 1 cut(s) 788
StyD4I CCNGG 3 cut(s) 76, 386, 1061
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 1 cut(s) 446
TaqI TCGA 6 cut(s) 99, 223, 298, 447, 1056, 1113
TasI AATT 6 cut(s) 318, 364, 808, 910, 946, 1159
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 3 cut(s) 101, 991, 1076
Tru1I TTAA 2 cut(s) 41, 819
Tru9I TTAA 2 cut(s) 41, 819
TscAI CASTG 3 cut(s) 42, 975, 1113
TseFI GTSAC 3 cut(s) 31, 187, 340
TseI GCWGC 4 cut(s) 391, 548, 638, 779
Tsp45I GTSAC 3 cut(s) 31, 187, 340
TspDTI ATGAA 2 cut(s) 249, 872
TspGWI ACGGA 2 cut(s) 66, 602
TspRI CASTG 3 cut(s) 42, 975, 1113
VpaK11BI GGWCC 1 cut(s) 347
XceI RCATGY 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 171, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.