Rroxscaffold_2G00138650

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001289
Physical Location & Seq
Forward (+)
76809787 .. 76811743
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138650.1

Sequence Viewer

Length: 828 bp
ATGTCGACTGGTTTGGCAAAGAGAAAGATGGTGACAGTGTTAAGCATTGATGGAGGTGGTATTAGAGGCATCATCCCTGGCACTATCCTCGGCTTTCTCGAATCCCAGCTTCAGGATTTGGATGGCCCGAGTGCAAGACTTGCAGATTATTTTGATATAATCGCCGGAACTAGCACGGGTGGGCTGGTCACCACCATGCTTACAGCTCCGAACAAGGACAATCGACCCATGTATGAAGCGAAGGACATCAATAACTTCTACTTGGAACAAACACCTAAGATTTTCCCTCAGAAGAGTCGAAATAACTTCTTGACATCAATTCAAAGTTTGGTTGGTGCGGTGACCGGACCAAAATACGACGGGAAGTACCTGCGAACATTGACGACTGGGCTGCTTGGCGACCTCACTATAAAAGAAACTCTAACCAATGTCATCATTCCAACGTTCGATATCAAACACCTTCAGCCTGTGATTTTCTCAACCATTGATGCAAAAGAAAGTTCTTTGAAAAATGCAAAGCTTTCAGATATCTGCGTCAGTACCTCTGCAGCTCCAACTTTTCTCCCCGCACACTACTTTGAGGTTACGGACAAACAGGGAACAACTCGTTCTTTTGATCTCATTGATGGTGGGGTCGCTGCAAACAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGCACGAGTCAGAGCCTTTGGATGCCACTCGGTTGCTAGTGCTCTCATTGGGCACCGGGTCCGCCAAGTCTGAAAGGAAGTACACCGCAGCCGAGGCCTCAAAATGGGGTTTGATCAATTGGTTGTTTAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

275

Amino Acids

29.92

Weight (kDa)

7.79

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 222 3.7e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
AccB1I GGYRCC 1 cut(s) 743
AccI GTMKAC 1 cut(s) 5
AciI CCGC 4 cut(s) 338, 567, 753, 777
AclI AACGTT 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 660
AcuI CTGAAG 2 cut(s) 95, 446
AfaI GTAC 3 cut(s) 368, 541, 773
AfiI CCNNNNNNNGG 2 cut(s) 112, 795
AgsI TTSAA 3 cut(s) 323, 508, 691
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 4 cut(s) 109, 206, 520, 551
AluI AGCT 4 cut(s) 109, 206, 520, 551
Alw21I GWGCWC 1 cut(s) 735
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 3 cut(s) 124, 660, 786
ApeKI GCWGC 4 cut(s) 391, 548, 638, 779
AspS9I GGNCC 3 cut(s) 125, 347, 750
AsuC2I CCSGG 1 cut(s) 748
AsuHPI GGTGA 3 cut(s) 43, 181, 352
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 2 cut(s) 347, 750
BaeGI GKGCMC 1 cut(s) 746
BalI TGGCCA 1 cut(s) 662
BanI GGYRCC 1 cut(s) 743
BauI CACGAG 1 cut(s) 695
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 4 cut(s) 378, 560, 625, 791
BccI CCATC 5 cut(s) 22, 44, 116, 620, 652
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 373, 407
BciT130I CCWGG 1 cut(s) 78
BclI TGATCA 1 cut(s) 804
BcnI CCSGG 1 cut(s) 748
BfaI CTAG 2 cut(s) 171, 728
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 378
BisI GCNGC 4 cut(s) 392, 549, 639, 780
BlsI GCNGC 4 cut(s) 393, 550, 640, 781
Bme1390I CCNGG 2 cut(s) 78, 748
Bme18I GGWCC 2 cut(s) 347, 750
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 3 cut(s) 125, 347, 750
BmiI GGNNCC 2 cut(s) 745, 751
BmrFI CCNGG 2 cut(s) 78, 748
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 3 cut(s) 78, 478, 703
BmuI ACTGGG 1 cut(s) 396
BpuMI CCSGG 1 cut(s) 748
BsaJI CCNNGG 3 cut(s) 76, 88, 783
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 795
Bse1I ACTGG 2 cut(s) 13, 391
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 76, 88, 783
BseGI GGATG 3 cut(s) 72, 127, 718
BseLI CCNNNNNNNGG 2 cut(s) 112, 795
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 2 cut(s) 13, 391
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 4 cut(s) 378, 560, 625, 791
BseYI CCCAGC 1 cut(s) 105
BshFI GGCC 3 cut(s) 126, 662, 788
BshNI GGYRCC 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 735
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 3 cut(s) 165, 345, 747
BslI CCNNNNNNNGG 2 cut(s) 112, 795
BsnI GGCC 3 cut(s) 126, 662, 788
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 735, 746
Bsp143I GATC 2 cut(s) 616, 804
BspACI CCGC 4 cut(s) 338, 567, 753, 777
BspANI GGCC 3 cut(s) 126, 662, 788
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 745, 751
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 378
BspT107I GGYRCC 1 cut(s) 743
BsrI ACTGG 2 cut(s) 13, 391
BssECI CCNNGG 3 cut(s) 76, 88, 783
BssMI GATC 2 cut(s) 616, 804
BssSI CACGAG 1 cut(s) 695
Bst2BI CACGAG 1 cut(s) 695
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 287
BstAPI GCANNNNNTGC 1 cut(s) 140
BstDEI CTNAG 2 cut(s) 276, 288
BstEII GGTNACC 2 cut(s) 187, 340
BstF5I GGATG 3 cut(s) 72, 127, 718
BstKTI GATC 2 cut(s) 619, 807
BstMBI GATC 2 cut(s) 616, 804
BstMWI GCNNNNNNNGC 2 cut(s) 140, 785
BstNI CCWGG 1 cut(s) 78
BstPI GGTNACC 2 cut(s) 187, 340
BstSCI CCNGG 2 cut(s) 76, 746
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 746
BstV1I GCAGC 4 cut(s) 378, 560, 625, 791
BsuRI GGCC 3 cut(s) 126, 662, 788
BtsCI GGATG 3 cut(s) 72, 127, 718
BtsIMutI CAGTG 1 cut(s) 42
BveI ACCTGC 1 cut(s) 378
Cfr13I GGNCC 3 cut(s) 125, 347, 750
CseI GACGC 1 cut(s) 523
Csp6I GTAC 3 cut(s) 367, 540, 772
CviAII CATG 2 cut(s) 196, 229
CviQI GTAC 3 cut(s) 367, 540, 772
DdeI CTNAG 2 cut(s) 276, 288
DpnI GATC 2 cut(s) 618, 806
DpnII GATC 2 cut(s) 616, 804
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
EciI GGCGGA 1 cut(s) 742
Eco147I AGGCCT 1 cut(s) 788
Eco32I GATATC 2 cut(s) 451, 529
Eco47I GGWCC 2 cut(s) 347, 750
Eco57I CTGAAG 2 cut(s) 95, 446
Eco88I CYCGRG 1 cut(s) 127
Eco91I GGTNACC 2 cut(s) 187, 340
EcoO65I GGTNACC 2 cut(s) 187, 340
EcoRII CCWGG 1 cut(s) 76
EcoRV GATATC 2 cut(s) 451, 529
FaeI CATG 2 cut(s) 199, 232
FaiI YATR 7 cut(s) 158, 197, 230, 234, 410, 665, 674
FatI CATG 2 cut(s) 195, 228
FauI CCCGC 1 cut(s) 574
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 392, 549, 639, 780
FokI GGATG 3 cut(s) 59, 134, 725
Fsp4HI GCNGC 4 cut(s) 392, 549, 639, 780
FspBI CTAG 2 cut(s) 171, 728
GluI GCNGC 4 cut(s) 392, 549, 639, 780
GsaI CCCAGC 1 cut(s) 109
HaeIII GGCC 3 cut(s) 126, 662, 788
HapII CCGG 3 cut(s) 165, 345, 747
HgaI GACGC 1 cut(s) 523
Hin1II CATG 2 cut(s) 199, 232
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 3 cut(s) 101, 295, 698
HpaII CCGG 3 cut(s) 165, 345, 747
HphI GGTGA 3 cut(s) 43, 181, 352
Hpy166II GTNNAC 2 cut(s) 6, 774
Hpy188I TCNGA 5 cut(s) 210, 291, 526, 703, 763
Hpy188III TCNNGA 3 cut(s) 98, 113, 310
Hpy8I GTNNAC 2 cut(s) 6, 774
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 2 cut(s) 235, 470
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 6 cut(s) 134, 143, 491, 515, 548, 641
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 785
HpyF3I CTNAG 2 cut(s) 276, 288
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 2 cut(s) 199, 232
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 2 cut(s) 616, 804
LmnI GCTCC 2 cut(s) 211, 556
Lsp1109I GCAGC 4 cut(s) 378, 560, 625, 791
LweI GCATC 3 cut(s) 78, 478, 703
MaeI CTAG 2 cut(s) 171, 728
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 4 cut(s) 31, 187, 340, 583
MalI GATC 2 cut(s) 618, 806
MboI GATC 2 cut(s) 616, 804
MboII GAAGA 1 cut(s) 304
MfeI CAATTG 1 cut(s) 808
MhlI GDGCHC 2 cut(s) 735, 746
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 3 cut(s) 318, 808, 823
MluNI TGGCCA 1 cut(s) 662
MlyI GAGTC 2 cut(s) 304, 707
MmeI TCCRAC 2 cut(s) 464, 578
MnlI CCTC 9 cut(s) 47, 59, 98, 297, 413, 553, 574, 778, 799
Mox20I TGGCCA 1 cut(s) 662
MscI TGGCCA 1 cut(s) 662
MseI TTAA 3 cut(s) 41, 819, 826
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 662
MspI CCGG 3 cut(s) 165, 345, 747
MspR9I CCNGG 2 cut(s) 78, 748
MunI CAATTG 1 cut(s) 808
MvaI CCWGG 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 140, 785
NciI CCSGG 1 cut(s) 748
NdeII GATC 2 cut(s) 616, 804
NlaIII CATG 2 cut(s) 199, 232
NlaIV GGNNCC 2 cut(s) 745, 751
NmeAIII GCCGAG 2 cut(s) 69, 808
NmuCI GTSAC 3 cut(s) 31, 187, 340
PceI AGGCCT 1 cut(s) 788
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 1 cut(s) 101
PkrI GCNGC 4 cut(s) 393, 550, 640, 781
PleI GAGTC 2 cut(s) 303, 706
PpsI GAGTC 2 cut(s) 303, 706
Psp1406I AACGTT 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 76
PspEI GGTNACC 2 cut(s) 187, 340
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 2 cut(s) 745, 751
PspPI GGNCC 3 cut(s) 125, 347, 750
PstI CTGCAG 1 cut(s) 550
RsaI GTAC 3 cut(s) 368, 541, 773
RsaNI GTAC 3 cut(s) 367, 540, 772
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 41, 819, 826
SatI GCNGC 4 cut(s) 392, 549, 639, 780
Sau3AI GATC 2 cut(s) 616, 804
Sau96I GGNCC 3 cut(s) 125, 347, 750
SchI GAGTC 2 cut(s) 304, 707
ScrFI CCNGG 2 cut(s) 78, 748
SduI GDGCHC 2 cut(s) 735, 746
SfaNI GCATC 3 cut(s) 78, 478, 703
SfcI CTRYAG 1 cut(s) 546
SinI GGWCC 2 cut(s) 347, 750
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 3 cut(s) 318, 808, 823
SseBI AGGCCT 1 cut(s) 788
SsiI CCGC 4 cut(s) 338, 567, 753, 777
SspMI CTAG 2 cut(s) 171, 728
StuI AGGCCT 1 cut(s) 788
StyD4I CCNGG 2 cut(s) 76, 746
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 1 cut(s) 446
TaqI TCGA 5 cut(s) 5, 99, 223, 298, 447
TasI AATT 3 cut(s) 318, 808, 823
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 1 cut(s) 101
Tru1I TTAA 3 cut(s) 41, 819, 826
Tru9I TTAA 3 cut(s) 41, 819, 826
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 3 cut(s) 31, 187, 340
TseI GCWGC 4 cut(s) 391, 548, 638, 779
Tsp45I GTSAC 3 cut(s) 31, 187, 340
TspDTI ATGAA 1 cut(s) 249
TspGWI ACGGA 1 cut(s) 602
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 2 cut(s) 347, 750
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 171, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.