pycom02g13510

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001289
Physical Location & Seq
Reverse (-)
10017575 .. 10020052
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13510.5

Sequence Viewer

Length: 1203 bp
ATGCTGATTACTGATATTGATTTACTAAATCTGAATCTGAAGATGTCCACTTGTATGACAAAGAGAAAGACAGTGACAGTTCTAAGCATCGATGGAGGTGGCATTAAAGGTCTAATTCCCAGCACCCTCCTCGCTTTTCTTGAATCCAAGCTTCAGGATTTGGATGGGCCTGATGCAAGAATTGCAGATTATTTCAACATAATTGCTGGAACAAGCACCGGTGGTCTTATCACCACCATGCTTACAGCTCCCAACAAAGACAATCGGCCCATGTACCAAGCAAAGGACATCACCACCTTCTATTTAAATCACTGCCCTAAGATTTTCCCTCAGAACAGAGGAAAAAACTTGTTGACATCAATAAAAAGTTTGGTTGGTGCTGTCATGGGGCCAAAATATGACGGCAAGTACCTGCGCACATTGTTGAGTGGTCTGCTTGGTGACCTCACTCTCAAAGACACTCTAACTAATGTGCTTATTCCATCTTTCGATATCAAGTACCTTCAGCCTGTCGTCTTCTCCACCATTGATGCAAAAGATCATCCTTTGAAAAATGCTAAGCTCTCGGATACCTGTGTAAGTACCTCTGCTGCTCCTACCTATCTCCCTGCACACTACTTCGACGTCAAGGATGACGAGGGAAGGACTCGCACTTTTGATCTCGTTGATGGAGGGGTTGCTGCAAACAATCCTACAATGATGGCCATAAGTCACATAAATAGAGAGATGTTGAGGAAAGGCTCGGATCCGATGGATGCGAAGAGGTTGTTAGTGCTATCGTTGGGCACAGGCACCGCCAAGTTCGAAGAGAAGTACAATGCAGCCACGGCCTCCAAATGGGGTTTGATCAATTGGGTCTTGCACAATGGGAACACACCTTTGGTAGACATTTTCAGCGATGCGAGTTCTGATATGGTTGACATTCATGTCTCCACCCTCTTCCAATGCGTCCACGCCAAGCACAACTATCTACGTATTCAGGATGACTCATTGACCGGTGATGAAGCATCAGTAGATATAGCAACGGAGAAGAATCTTATGAGACTTATAGAGATCGGGAATGCGTTGTTGAAGAAGCCAGTTTCACGGGTGCAGTTAGATACCGGAAGGTATGAGAAACCTGAGGGAGAAGAGGGTACCTATGAAGAAGCACTGATCGATTTTGCCAAAAGGTTATCAGAAGTAAAGAAGACTGCAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

401

Amino Acids

43.99

Weight (kDa)

6.46

Isoelectric Point (pI)

29.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 926
AatII GACGTC 1 cut(s) 627
Acc16I TGCGCA 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 420
Acc65I GGTACC 1 cut(s) 1136
AccB1I GGYRCC 2 cut(s) 791, 1136
AccI GTMKAC 1 cut(s) 885
AciI CCGC 1 cut(s) 795
AclWI GGATC 2 cut(s) 740, 753
AcoI YGGCCR 1 cut(s) 702
AcuI CTGAAG 3 cut(s) 59, 137, 488
AcyI GRCGYC 1 cut(s) 624
AfaI GTAC 6 cut(s) 275, 410, 500, 583, 815, 1138
AfiI CCNNNNNNNGG 2 cut(s) 283, 837
AgeI ACCGGT 2 cut(s) 218, 995
AgsI TTSAA 4 cut(s) 143, 196, 550, 1072
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
AluBI AGCT 3 cut(s) 151, 248, 562
AluI AGCT 3 cut(s) 151, 248, 562
Alw26I GTCTC 2 cut(s) 934, 1036
AlwI GGATC 2 cut(s) 740, 753
AoxI GGCC 5 cut(s) 167, 266, 389, 702, 828
ApeKI GCWGC 3 cut(s) 590, 680, 821
AsiGI ACCGGT 2 cut(s) 218, 995
Asp718I GGTACC 1 cut(s) 1136
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 3 cut(s) 167, 267, 389
AsuHPI GGTGA 4 cut(s) 223, 283, 452, 1010
AsuII TTCGAA 1 cut(s) 804
AxyI CCTNAGG 1 cut(s) 1122
BaeGI GKGCMC 1 cut(s) 788
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BalI TGGCCA 1 cut(s) 704
BamHI GGATCC 1 cut(s) 745
BanI GGYRCC 2 cut(s) 791, 1136
BbsI GAAGAC 2 cut(s) 508, 1196
BbvI GCAGC 3 cut(s) 577, 667, 833
BccI CCATC 6 cut(s) 86, 158, 490, 662, 694, 745
BceAI ACGGC 2 cut(s) 418, 843
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciVI GTATCC 1 cut(s) 562
BclI TGATCA 1 cut(s) 846
BcoDI GTCTC 2 cut(s) 934, 1036
BfmI CTRYAG 1 cut(s) 1194
BfuAI ACCTGC 1 cut(s) 420
BfuI GTATCC 1 cut(s) 562
BisI GCNGC 3 cut(s) 591, 681, 822
BlpI GCTNAGC 1 cut(s) 558
BlsI GCNGC 3 cut(s) 592, 682, 823
BmgT120I GGNCC 3 cut(s) 167, 267, 389
BmiI GGNNCC 4 cut(s) 390, 747, 793, 1138
BmsI GCATC 6 cut(s) 96, 163, 520, 745, 889, 1016
BpiI GAAGAC 2 cut(s) 508, 1196
Bpu1102I GCTNAGC 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 804
Bsa29I ATCGAT 2 cut(s) 90, 1158
BsaAI YACGTR 1 cut(s) 974
BsaHI GRCGYC 1 cut(s) 624
BsaJI CCNNGG 1 cut(s) 825
BsaWI WCCGGW 3 cut(s) 218, 995, 1103
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 837
Bse118I RCCGGY 2 cut(s) 218, 995
Bse1I ACTGG 1 cut(s) 1079
Bse21I CCTNAGG 1 cut(s) 1122
BseCI ATCGAT 2 cut(s) 90, 1158
BseDI CCNNGG 1 cut(s) 825
BseGI GGATG 5 cut(s) 169, 541, 637, 760, 988
BseLI CCNNNNNNNGG 2 cut(s) 283, 837
BseMII CTCAG 2 cut(s) 344, 1113
BseNI ACTGG 1 cut(s) 1079
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 788
BseXI GCAGC 3 cut(s) 577, 667, 833
BseYI CCCAGC 1 cut(s) 119
BsgI GTGCAG 2 cut(s) 594, 1112
BshFI GGCC 5 cut(s) 169, 268, 391, 704, 830
BshNI GGYRCC 2 cut(s) 791, 1136
BshTI ACCGGT 2 cut(s) 218, 995
BshVI ATCGAT 2 cut(s) 90, 1158
BsiSI CCGG 3 cut(s) 219, 996, 1104
BslI CCNNNNNNNGG 2 cut(s) 283, 837
BsmAI GTCTC 2 cut(s) 934, 1036
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 5 cut(s) 169, 268, 391, 704, 830
Bsp119I TTCGAA 1 cut(s) 804
Bsp1286I GDGCHC 1 cut(s) 788
Bsp143I GATC 6 cut(s) 538, 658, 745, 846, 1053, 1155
Bsp1720I GCTNAGC 1 cut(s) 558
BspACI CCGC 1 cut(s) 795
BspANI GGCC 5 cut(s) 169, 268, 391, 704, 830
BspCNI CTCAG 2 cut(s) 343, 1114
BspDI ATCGAT 2 cut(s) 90, 1158
BspLI GGNNCC 4 cut(s) 390, 747, 793, 1138
BspMAI CTGCAG 1 cut(s) 1198
BspMI ACCTGC 1 cut(s) 420
BspPI GGATC 2 cut(s) 740, 753
BspT104I TTCGAA 1 cut(s) 804
BspT107I GGYRCC 2 cut(s) 791, 1136
BsrFI RCCGGY 2 cut(s) 218, 995
BsrI ACTGG 1 cut(s) 1079
BssAI RCCGGY 2 cut(s) 218, 995
BssECI CCNNGG 1 cut(s) 825
BssMI GATC 6 cut(s) 538, 658, 745, 846, 1053, 1155
BssNI GRCGYC 1 cut(s) 624
Bst4CI ACNGT 2 cut(s) 73, 79
Bst6I CTCTTC 4 cut(s) 755, 801, 944, 1125
BstACI GRCGYC 1 cut(s) 624
BstAPI GCANNNNNTGC 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 974
BstBI TTCGAA 1 cut(s) 804
BstDEI CTNAG 5 cut(s) 83, 318, 330, 558, 1122
BstDSI CCRYGG 1 cut(s) 825
BstEII GGTNACC 1 cut(s) 440
BstF5I GGATG 5 cut(s) 169, 541, 637, 760, 988
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 6 cut(s) 541, 661, 748, 849, 1056, 1158
BstMAI GTCTC 2 cut(s) 934, 1036
BstMBI GATC 6 cut(s) 538, 658, 745, 846, 1053, 1155
BstMWI GCNNNNNNNGC 2 cut(s) 182, 827
BstPI GGTNACC 1 cut(s) 440
BstSFI CTRYAG 1 cut(s) 1194
BstSLI GKGCMC 1 cut(s) 788
BstSNI TACGTA 1 cut(s) 974
BstV1I GCAGC 3 cut(s) 577, 667, 833
BstV2I GAAGAC 2 cut(s) 508, 1196
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
Bsu15I ATCGAT 2 cut(s) 90, 1158
Bsu36I CCTNAGG 1 cut(s) 1122
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 5 cut(s) 169, 268, 391, 704, 830
BsuTUI ATCGAT 2 cut(s) 90, 1158
BtgI CCRYGG 1 cut(s) 825
BtgZI GCGATG 1 cut(s) 912
BtsCI GGATG 5 cut(s) 169, 541, 637, 760, 988
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 3 cut(s) 78, 310, 1151
BveI ACCTGC 1 cut(s) 420
CfoI GCGC 1 cut(s) 417
Cfr10I RCCGGY 2 cut(s) 218, 995
Cfr13I GGNCC 3 cut(s) 167, 267, 389
ClaI ATCGAT 2 cut(s) 90, 1158
CseI GACGC 1 cut(s) 937
Csp6I GTAC 6 cut(s) 274, 409, 499, 582, 814, 1137
CspAI ACCGGT 2 cut(s) 218, 995
CviAII CATG 4 cut(s) 238, 271, 385, 926
CviQI GTAC 6 cut(s) 274, 409, 499, 582, 814, 1137
DdeI CTNAG 5 cut(s) 83, 318, 330, 558, 1122
DpnI GATC 6 cut(s) 540, 660, 747, 848, 1055, 1157
DpnII GATC 6 cut(s) 538, 658, 745, 846, 1053, 1155
DraI TTTAAA 1 cut(s) 306
DrdI GACNNNNNNGTC 1 cut(s) 926
DseDI GACNNNNNNGTC 1 cut(s) 926
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 4 cut(s) 755, 801, 944, 1125
EarI CTCTTC 4 cut(s) 755, 801, 944, 1125
Eco105I TACGTA 1 cut(s) 974
Eco32I GATATC 1 cut(s) 493
Eco57I CTGAAG 3 cut(s) 59, 137, 488
Eco81I CCTNAGG 1 cut(s) 1122
Eco91I GGTNACC 1 cut(s) 440
EcoO65I GGTNACC 1 cut(s) 440
EcoRV GATATC 1 cut(s) 493
FaeI CATG 4 cut(s) 241, 274, 388, 929
FatI CATG 4 cut(s) 237, 270, 384, 925
FbaI TGATCA 1 cut(s) 846
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 3 cut(s) 591, 681, 822
FokI GGATG 5 cut(s) 176, 528, 644, 767, 995
Fsp4HI GCNGC 3 cut(s) 591, 681, 822
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 1 cut(s) 416
GluI GCNGC 3 cut(s) 591, 681, 822
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 5 cut(s) 169, 268, 391, 704, 830
HapII CCGG 3 cut(s) 219, 996, 1104
HgaI GACGC 1 cut(s) 937
HhaI GCGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 624
Hin1II CATG 4 cut(s) 241, 274, 388, 929
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 2 cut(s) 354, 919
HindII GTYRAC 2 cut(s) 354, 919
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 5 cut(s) 34, 143, 646, 986, 1033
HpaII CCGG 3 cut(s) 219, 996, 1104
HphI GGTGA 4 cut(s) 223, 283, 452, 1010
Hpy166II GTNNAC 5 cut(s) 48, 354, 886, 919, 952
Hpy188I TCNGA 8 cut(s) 33, 39, 333, 568, 745, 750, 910, 1180
Hpy188III TCNNGA 4 cut(s) 140, 155, 980, 1057
Hpy8I GTNNAC 5 cut(s) 48, 354, 886, 919, 952
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 4 cut(s) 307, 512, 636, 1101
HpyCH4III ACNGT 2 cut(s) 73, 79
HpyCH4IV ACGT 2 cut(s) 624, 973
HpyCH4V TGCA 9 cut(s) 176, 185, 533, 611, 683, 821, 862, 1093, 1196
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 827
HpyF3I CTNAG 5 cut(s) 83, 318, 330, 558, 1122
HpySE526I ACGT 2 cut(s) 624, 973
Hsp92I GRCGYC 1 cut(s) 624
Hsp92II CATG 4 cut(s) 241, 274, 388, 929
HspAI GCGC 1 cut(s) 415
KpnI GGTACC 1 cut(s) 1140
Ksp22I TGATCA 1 cut(s) 846
Kzo9I GATC 6 cut(s) 538, 658, 745, 846, 1053, 1155
LmnI GCTCC 2 cut(s) 253, 598
Lsp1109I GCAGC 3 cut(s) 577, 667, 833
LweI GCATC 6 cut(s) 96, 163, 520, 745, 889, 1016
MaeII ACGT 2 cut(s) 624, 973
MaeIII GTNAC 3 cut(s) 73, 440, 710
MalI GATC 6 cut(s) 540, 660, 747, 848, 1055, 1157
MboI GATC 6 cut(s) 538, 658, 745, 846, 1053, 1155
MfeI CAATTG 1 cut(s) 850
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 1 cut(s) 788
MlsI TGGCCA 1 cut(s) 704
MluCI AATT 4 cut(s) 114, 180, 201, 850
MluNI TGGCCA 1 cut(s) 704
MlyI GAGTC 2 cut(s) 640, 980
Mox20I TGGCCA 1 cut(s) 704
MscI TGGCCA 1 cut(s) 704
MseI TTAA 2 cut(s) 105, 305
MslI CAYNNNNRTG 2 cut(s) 53, 236
Msp20I TGGCCA 1 cut(s) 704
MspI CCGG 3 cut(s) 219, 996, 1104
MunI CAATTG 1 cut(s) 850
Mva1269I GAATGC 1 cut(s) 1066
MwoI GCNNNNNNNGC 2 cut(s) 182, 827
NdeII GATC 6 cut(s) 538, 658, 745, 846, 1053, 1155
NlaIII CATG 4 cut(s) 241, 274, 388, 929
NlaIV GGNNCC 4 cut(s) 390, 747, 793, 1138
NmuCI GTSAC 3 cut(s) 73, 440, 710
NsbI TGCGCA 1 cut(s) 416
NspV TTCGAA 1 cut(s) 804
PctI GAATGC 1 cut(s) 1066
PfeI GAWTC 3 cut(s) 34, 143, 1033
PinAI ACCGGT 2 cut(s) 218, 995
PkrI GCNGC 3 cut(s) 592, 682, 823
PleI GAGTC 2 cut(s) 640, 980
PpsI GAGTC 2 cut(s) 640, 980
Ppu21I YACGTR 1 cut(s) 974
PspEI GGTNACC 1 cut(s) 440
PspFI CCCAGC 1 cut(s) 119
PspN4I GGNNCC 4 cut(s) 390, 747, 793, 1138
PspPI GGNCC 3 cut(s) 167, 267, 389
PstI CTGCAG 1 cut(s) 1198
PsuI RGATCY 1 cut(s) 745
RsaI GTAC 6 cut(s) 275, 410, 500, 583, 815, 1138
RsaNI GTAC 6 cut(s) 274, 409, 499, 582, 814, 1137
RseI CAYNNNNRTG 2 cut(s) 53, 236
SaqAI TTAA 2 cut(s) 105, 305
SatI GCNGC 3 cut(s) 591, 681, 822
Sau3AI GATC 6 cut(s) 538, 658, 745, 846, 1053, 1155
Sau96I GGNCC 3 cut(s) 167, 267, 389
SchI GAGTC 2 cut(s) 640, 980
SduI GDGCHC 1 cut(s) 788
SfaNI GCATC 6 cut(s) 96, 163, 520, 745, 889, 1016
SfcI CTRYAG 1 cut(s) 1194
SfuI TTCGAA 1 cut(s) 804
SgrAI CRCCGGYG 1 cut(s) 218
SmiI ATTTAAAT 1 cut(s) 306
SmiMI CAYNNNNRTG 2 cut(s) 53, 236
SnaBI TACGTA 1 cut(s) 974
Sse9I AATT 4 cut(s) 114, 180, 201, 850
SsiI CCGC 1 cut(s) 795
SwaI ATTTAAAT 1 cut(s) 306
TaaI ACNGT 2 cut(s) 73, 79
TaiI ACGT 2 cut(s) 627, 976
TaqI TCGA 5 cut(s) 90, 489, 621, 804, 1158
TasI AATT 4 cut(s) 114, 180, 201, 850
TatI WGTACW 1 cut(s) 813
TfiI GAWTC 3 cut(s) 34, 143, 1033
Tru1I TTAA 2 cut(s) 105, 305
Tru9I TTAA 2 cut(s) 105, 305
TscAI CASTG 3 cut(s) 78, 317, 1158
TseFI GTSAC 3 cut(s) 73, 440, 710
TseI GCWGC 3 cut(s) 590, 680, 821
Tsp45I GTSAC 3 cut(s) 73, 440, 710
TspDTI ATGAA 3 cut(s) 914, 1017, 1158
TspGWI ACGGA 1 cut(s) 1040
TspRI CASTG 3 cut(s) 78, 317, 1158
XmiI GTMKAC 1 cut(s) 885
ZraI GACGTC 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.