Prupe.7G137000_v2.0.a1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001289
Physical Location & Seq
Forward (+)
15402310 .. 15404505
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G137000.2

Sequence Viewer

Length: 1161 bp
ATGTCGACTGGTTTGACAAAGAGAAGGATGGTGACAGTGCTAAGCATTGATGGAGGTGGCATTAGAGGCATAATTCCCAGCACCCTCTTGGCCTTCCTTGAATCCAAGCTTCAGGAATTGGATGGGCCAAATGCAAGAATTGCAGATTATTTCGACATAATTGCCGGAACAAGCACCGGTGGTCTTGTCACCACCATGCTTACAGCTCCCAACAAGGACAACCGACCCATGTATGAAGCAAAGGACATCAACAAATTCTATCTGGATAACACCCCTAAGATTTTCCCTCAAAATAGTCGAAACAACTTCTTGACATCAATAACAAGTATGGTTGGTGCGGTTATGGGGCCTAAATATGATGGGAAGTACTTGCGTACATTGGTAAATGGGTTGCTTGGTGATCTCACTCTCAAGCAGACTTTAACCACTGTGATTATTCCAACATTTGATATCAAGCACCTTCAGCCTGTGATCTTCTCAACCACCGATGCGAAAGAAAGTGCTCTGAAAAATGCCAAGCTTTCGGATATCTGCATAAGCACCTCTGCTGCTCCTACTTTTCTCCCAGCACACTACTTCGAGGTCAATAGTGAGGGAAGGACCCGAACTTTTGATCTCATTGATGGCGGGGTTGCTGCAAACAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATATTGAAGCACGATTCGGAGCCGATGGATGCGAGTAGATTGCTAGTGCTGTCATTGGGCACAGGTGCAGCCAAGTTTGAAGGGAAGTACAATGCAGCCATGGCCTCCAAATGGGGTTTGATCAGTTGGATGTTTGACAATGGGAGCACACCTTTGGTGGACGTTTTTAATGATGCAAGTTCTGATATGGTTGACATTCATGTGTCCACCCTCTTCCAATCCACCCACGCCAAGGACAACTACTTACGCATTCAGGATGATTCGTTAAGCGGTGATGAAGTATCGGTGGATATAGCGACAGAGAAGAATCTAAATAGGCTTGTGGAGATTGGAAATGAGCTGCTGAAGAAGCGAGTGTCACGGGTGAATTTGGATATAGGGAGGTATGAGGAAGTTGAGGGAGAGGGTACTTATGAAGAAGCACTGGTCGATTTTGCCAAAAGGCTCTCGGATGCAAAGAAGATTGGACAAAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

387

Amino Acids

42.36

Weight (kDa)

5.63

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 338, 627, 951
AcoI YGGCCR 1 cut(s) 657
AcsI RAATTY 2 cut(s) 254, 1048
AcuI CTGAAG 3 cut(s) 95, 446, 1046
AfaI GTAC 4 cut(s) 368, 376, 770, 1090
AfiI CCNNNNNNNGG 2 cut(s) 792, 913
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 3 cut(s) 101, 688, 761
AluBI AGCT 4 cut(s) 109, 206, 520, 1021
AluI AGCT 4 cut(s) 109, 206, 520, 1021
Alw21I GWGCWC 2 cut(s) 505, 830
AoxI GGCC 5 cut(s) 90, 125, 347, 657, 783
ApeKI GCWGC 5 cut(s) 548, 635, 749, 776, 1021
ApoI RAATTY 2 cut(s) 254, 1048
AsiGI ACCGGT 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 125, 347, 600
AsuHPI GGTGA 5 cut(s) 43, 181, 410, 965, 1057
AvaII GGWCC 1 cut(s) 600
BaeGI GKGCMC 1 cut(s) 743
BalI TGGCCA 1 cut(s) 659
Bbv12I GWGCWC 2 cut(s) 505, 830
BbvI GCAGC 5 cut(s) 535, 622, 761, 788, 1008
BccI CCATC 7 cut(s) 22, 44, 116, 353, 617, 649, 700
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 703, 737
BclI TGATCA 1 cut(s) 801
BfaI CTAG 1 cut(s) 725
BisI GCNGC 5 cut(s) 549, 636, 750, 777, 1022
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 5 cut(s) 550, 637, 751, 778, 1023
BmcAI AGTACT 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 600
BmgT120I GGNCC 3 cut(s) 125, 347, 600
BmiI GGNNCC 3 cut(s) 348, 602, 702
BmsI GCATC 4 cut(s) 478, 700, 844, 1123
Bpu1102I GCTNAGC 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 780, 912
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 2 cut(s) 792, 913
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 2 cut(s) 13, 1110
BseDI CCNNGG 2 cut(s) 780, 912
BseGI GGATG 6 cut(s) 33, 127, 715, 816, 943, 1138
BseLI CCNNNNNNNGG 2 cut(s) 792, 913
BseNI ACTGG 2 cut(s) 13, 1110
BseSI GKGCMC 1 cut(s) 743
BseXI GCAGC 5 cut(s) 535, 622, 761, 788, 1008
BseYI CCCAGC 2 cut(s) 77, 565
BsgI GTGCAG 1 cut(s) 768
BshFI GGCC 5 cut(s) 92, 127, 349, 659, 785
BshTI ACCGGT 1 cut(s) 176
BsiHKAI GWGCWC 2 cut(s) 505, 830
BsiSI CCGG 2 cut(s) 165, 177
BslI CCNNNNNNNGG 2 cut(s) 792, 913
BsmI GAATGC 1 cut(s) 930
BsnI GGCC 5 cut(s) 92, 127, 349, 659, 785
Bsp1286I GDGCHC 3 cut(s) 505, 743, 830
Bsp143I GATC 4 cut(s) 400, 471, 613, 801
Bsp1720I GCTNAGC 1 cut(s) 41
Bsp19I CCATGG 1 cut(s) 780
BspACI CCGC 3 cut(s) 338, 627, 951
BspANI GGCC 5 cut(s) 92, 127, 349, 659, 785
BspLI GGNNCC 3 cut(s) 348, 602, 702
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 2 cut(s) 13, 1110
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 2 cut(s) 780, 912
BssMI GATC 4 cut(s) 400, 471, 613, 801
BssT1I CCWWGG 2 cut(s) 780, 912
Bst4CI ACNGT 2 cut(s) 37, 430
Bst6I CTCTTC 1 cut(s) 899
BstAPI GCANNNNNTGC 1 cut(s) 140
BstDEI CTNAG 2 cut(s) 41, 276
BstDSI CCRYGG 1 cut(s) 780
BstF5I GGATG 6 cut(s) 33, 127, 715, 816, 943, 1138
BstKTI GATC 4 cut(s) 403, 474, 616, 804
BstMBI GATC 4 cut(s) 400, 471, 613, 801
BstMWI GCNNNNNNNGC 5 cut(s) 66, 140, 463, 782, 1030
BstSLI GKGCMC 1 cut(s) 743
BstV1I GCAGC 5 cut(s) 535, 622, 761, 788, 1008
BsuRI GGCC 5 cut(s) 92, 127, 349, 659, 785
BtgI CCRYGG 1 cut(s) 780
BtsCI GGATG 6 cut(s) 33, 127, 715, 816, 943, 1138
BtsIMutI CAGTG 3 cut(s) 42, 426, 1103
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 3 cut(s) 125, 347, 600
Csp6I GTAC 4 cut(s) 367, 375, 769, 1089
CspAI ACCGGT 1 cut(s) 176
CviAII CATG 4 cut(s) 196, 229, 781, 881
CviQI GTAC 4 cut(s) 367, 375, 769, 1089
DdeI CTNAG 2 cut(s) 41, 276
DpnI GATC 4 cut(s) 402, 473, 615, 803
DpnII GATC 4 cut(s) 400, 471, 613, 801
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 1 cut(s) 899
EarI CTCTTC 1 cut(s) 899
Eco130I CCWWGG 2 cut(s) 780, 912
Eco32I GATATC 2 cut(s) 451, 529
Eco47I GGWCC 1 cut(s) 600
Eco57I CTGAAG 3 cut(s) 95, 446, 1046
EcoO109I RGGNCCY 2 cut(s) 347, 600
EcoRV GATATC 2 cut(s) 451, 529
EcoT14I CCWWGG 2 cut(s) 780, 912
ErhI CCWWGG 2 cut(s) 780, 912
FaeI CATG 4 cut(s) 199, 232, 784, 884
FatI CATG 4 cut(s) 195, 228, 780, 880
FauI CCCGC 1 cut(s) 620
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 549, 636, 750, 777, 1022
FokI GGATG 6 cut(s) 40, 134, 722, 823, 950, 1145
Fsp4HI GCNGC 5 cut(s) 549, 636, 750, 777, 1022
FspBI CTAG 1 cut(s) 725
GluI GCNGC 5 cut(s) 549, 636, 750, 777, 1022
GsaI CCCAGC 2 cut(s) 81, 569
HaeIII GGCC 5 cut(s) 92, 127, 349, 659, 785
HapII CCGG 2 cut(s) 165, 177
Hin1II CATG 4 cut(s) 199, 232, 784, 884
HincII GTYRAC 2 cut(s) 6, 874
HindII GTYRAC 2 cut(s) 6, 874
HindIII AAGCTT 2 cut(s) 107, 518
HinfI GANTC 4 cut(s) 101, 695, 941, 988
HpaII CCGG 2 cut(s) 165, 177
HphI GGTGA 5 cut(s) 43, 181, 410, 965, 1057
Hpy166II GTNNAC 4 cut(s) 6, 841, 874, 888
Hpy188I TCNGA 5 cut(s) 507, 526, 700, 865, 1132
Hpy188III TCNNGA 4 cut(s) 113, 263, 310, 935
Hpy8I GTNNAC 4 cut(s) 6, 841, 874, 888
HpyAV CCTTC 5 cut(s) 18, 103, 470, 591, 755
HpyCH4III ACNGT 2 cut(s) 37, 430
HpyCH4IV ACGT 1 cut(s) 843
HpyCH4V TGCA 8 cut(s) 134, 143, 534, 638, 749, 776, 857, 1136
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 140, 463, 782, 1030
HpyF3I CTNAG 2 cut(s) 41, 276
HpySE526I ACGT 1 cut(s) 843
Hsp92II CATG 4 cut(s) 199, 232, 784, 884
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 4 cut(s) 400, 471, 613, 801
LmnI GCTCC 4 cut(s) 211, 556, 700, 825
Lsp1109I GCAGC 5 cut(s) 535, 622, 761, 788, 1008
LweI GCATC 4 cut(s) 478, 700, 844, 1123
MaeI CTAG 1 cut(s) 725
MaeII ACGT 1 cut(s) 843
MaeIII GTNAC 3 cut(s) 31, 187, 1038
MalI GATC 4 cut(s) 402, 473, 615, 803
MboI GATC 4 cut(s) 400, 471, 613, 801
MboII GAAGA 6 cut(s) 466, 886, 997, 1039, 1109, 1153
MhlI GDGCHC 3 cut(s) 505, 743, 830
MlsI TGGCCA 1 cut(s) 659
MluCI AATT 6 cut(s) 72, 116, 138, 159, 254, 1048
MluNI TGGCCA 1 cut(s) 659
MmeI TCCRAC 2 cut(s) 464, 788
Mox20I TGGCCA 1 cut(s) 659
MscI TGGCCA 1 cut(s) 659
MseI TTAA 3 cut(s) 422, 849, 947
MslI CAYNNNNRTG 2 cut(s) 194, 881
Msp20I TGGCCA 1 cut(s) 659
MspI CCGG 2 cut(s) 165, 177
Mva1269I GAATGC 1 cut(s) 930
MwoI GCNNNNNNNGC 5 cut(s) 66, 140, 463, 782, 1030
NcoI CCATGG 1 cut(s) 780
NdeII GATC 4 cut(s) 400, 471, 613, 801
NlaIII CATG 4 cut(s) 199, 232, 784, 884
NlaIV GGNNCC 3 cut(s) 348, 602, 702
NmuCI GTSAC 3 cut(s) 31, 187, 1038
PctI GAATGC 1 cut(s) 930
PfeI GAWTC 4 cut(s) 101, 695, 941, 988
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 5 cut(s) 550, 637, 751, 778, 1023
PpuMI RGGWCCY 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 600
PspFI CCCAGC 2 cut(s) 77, 565
PspN4I GGNNCC 3 cut(s) 348, 602, 702
PspPI GGNCC 3 cut(s) 125, 347, 600
PspPPI RGGWCCY 1 cut(s) 600
RsaI GTAC 4 cut(s) 368, 376, 770, 1090
RsaNI GTAC 4 cut(s) 367, 375, 769, 1089
RseI CAYNNNNRTG 2 cut(s) 194, 881
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 422, 849, 947
SatI GCNGC 5 cut(s) 549, 636, 750, 777, 1022
Sau3AI GATC 4 cut(s) 400, 471, 613, 801
Sau96I GGNCC 3 cut(s) 125, 347, 600
ScaI AGTACT 1 cut(s) 368
SduI GDGCHC 3 cut(s) 505, 743, 830
SfaNI GCATC 4 cut(s) 478, 700, 844, 1123
SgrAI CRCCGGYG 1 cut(s) 176
SinI GGWCC 1 cut(s) 600
SmiMI CAYNNNNRTG 2 cut(s) 194, 881
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 6 cut(s) 72, 116, 138, 159, 254, 1048
SsiI CCGC 3 cut(s) 338, 627, 951
SspMI CTAG 1 cut(s) 725
StyI CCWWGG 2 cut(s) 780, 912
TaaI ACNGT 2 cut(s) 37, 430
TaiI ACGT 1 cut(s) 846
TaqI TCGA 5 cut(s) 5, 153, 298, 579, 1110
TasI AATT 6 cut(s) 72, 116, 138, 159, 254, 1048
TatI WGTACW 2 cut(s) 366, 768
TfiI GAWTC 4 cut(s) 101, 695, 941, 988
Tru1I TTAA 3 cut(s) 422, 849, 947
Tru9I TTAA 3 cut(s) 422, 849, 947
TscAI CASTG 3 cut(s) 42, 433, 1110
TseFI GTSAC 3 cut(s) 31, 187, 1038
TseI GCWGC 5 cut(s) 548, 635, 749, 776, 1021
Tsp45I GTSAC 3 cut(s) 31, 187, 1038
TspDTI ATGAA 4 cut(s) 249, 869, 972, 1110
TspRI CASTG 3 cut(s) 42, 433, 1110
VpaK11BI GGWCC 1 cut(s) 600
XapI RAATTY 2 cut(s) 254, 1048
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 725
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.