Rw2G013700

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001289
Physical Location & Seq
Reverse (-)
14829014 .. 14836399
7386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013700.1

Sequence Viewer

Length: 1107 bp
ATGTCCATTGGGTTGGCAAAGAGAAAGATGGTGACAGTTCTGAGCATTGATGGAGGTGGCATTAGAGGCATAATTCCCGGCACCATCCTCGGCTTTCTCGAATCTAAGCTTCAGGATTGGGATGGAGCAAATGCAAGAATTGCAGATTATTTTGACATAATCGCCGGAACCAGCACCGGTGGATTGGACATCAACAACTTTTATTTAGAACACTGTCCCCAGATTTTCCCTCAAAAAAGTCGGAAAAAGAAAAACTTCTTCACATCGATAACAAATGCGATGAAGCGGGGGCCAAAATACGATGGGATGTATCTGCGCTCATTGATAAACGGGCTCCTTGGTGATCTCACTCTCAAGCAGACTTTAACCAATGTGGTCATTCCAAGTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCTCAACCATCGATGCAAAAGAAAATTCATTGAAGAACGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATATTATCTCCCTCCACACTACTTCGAAGTCAAGGATGACCAGGGAAATATCCGCAAATTTGATCTTGTCGATGGTGGGGTTGCTGCAAATAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAAATGTTGAAGAACAACTCAGAACCAATAAATGCGAATCGCTTGCAAGTGCTGTCATTGGGCACTGGCGCTGCCAAGTTTGAGGAGAAGTACAGTGCAGTCGAGGCATCAAAATGGGGTTTGGTGAATTGGATGTTTAATAATGGGAGCACACCTTTGCTGGACATTTTTGGGGATGCCAGTTCTGATATGGTTGACTTTCATGTCTCCACTCTCTTCCAGTCCCTCCATGTCAAGGACAATTACCTACGAATTCAGGATGACACACTGAGCGGAGATGAATCATCAGTGGATATTGCAACCGAGAAGAATCTGAAAAGCCTTGTGGAGATCGGAAATGGGTTGCTGAAGAAGCCAGTGTCAAGGGTAAATCTCGATACTGGGAGGTACGAGGAATCTGAGGGAGACGGTACCTACGAAGAAGCACTGGTCGATTTTGCCAAACGGCTCGCGGAGGGAAAGAAGCTCGGGCAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

368

Amino Acids

40.57

Weight (kDa)

6.12

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 203 4.3e-23 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 827
Acc65I GGTACC 1 cut(s) 1034
AccB1I GGYRCC 2 cut(s) 80, 1034
AccBSI CCGCTC 1 cut(s) 897
AccII CGCG 1 cut(s) 1076
AciI CCGC 4 cut(s) 286, 550, 897, 1076
AcoI YGGCCR 1 cut(s) 603
AcsI RAATTY 3 cut(s) 442, 554, 876
AcuI CTGAAG 3 cut(s) 95, 389, 992
AfaI GTAC 4 cut(s) 484, 716, 1013, 1036
AfiI CCNNNNNNNGG 1 cut(s) 859
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 2 cut(s) 451, 634
AjnI CCWGG 1 cut(s) 537
AluBI AGCT 4 cut(s) 109, 463, 494, 1090
AluI AGCT 4 cut(s) 109, 463, 494, 1090
Alw21I GWGCWC 1 cut(s) 776
Alw26I GTCTC 2 cut(s) 835, 1023
Ama87I CYCGRG 1 cut(s) 1091
AoxI GGCC 2 cut(s) 290, 603
ApeKI GCWGC 3 cut(s) 491, 581, 695
ApoI RAATTY 3 cut(s) 442, 554, 876
AsiGI ACCGGT 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 254
Asp718I GGTACC 1 cut(s) 1034
AspLEI GCGC 2 cut(s) 318, 695
AspS9I GGNCC 1 cut(s) 290
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 3 cut(s) 43, 353, 760
AsuII TTCGAA 1 cut(s) 522
AvaI CYCGRG 1 cut(s) 1091
BaeGI GKGCMC 1 cut(s) 689
BalI TGGCCA 1 cut(s) 605
BanI GGYRCC 2 cut(s) 80, 1034
BanII GRGCYC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 776
BbvI GCAGC 3 cut(s) 503, 568, 682
BccI CCATC 8 cut(s) 22, 44, 92, 116, 296, 434, 563, 595
BceAI ACGGC 1 cut(s) 1085
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 649, 683
BciT130I CCWGG 1 cut(s) 539
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 2 cut(s) 835, 1023
BfmI CTRYAG 1 cut(s) 489
BfoI RGCGCY 1 cut(s) 696
BisI GCNGC 3 cut(s) 492, 582, 696
BlsI GCNGC 3 cut(s) 493, 583, 697
Bme1390I CCNGG 2 cut(s) 78, 539
BmeT110I CYCGRG 1 cut(s) 1091
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 5 cut(s) 82, 169, 291, 335, 1036
BmrFI CCNGG 2 cut(s) 78, 539
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 4 cut(s) 421, 486, 740, 790
BmuI ACTGGG 1 cut(s) 1014
Bpu14I TTCGAA 1 cut(s) 522
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 78
Bsa29I ATCGAT 2 cut(s) 266, 429
BsaJI CCNNGG 3 cut(s) 88, 337, 538
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 1 cut(s) 859
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 6 cut(s) 694, 804, 844, 980, 1009, 1056
BseBI CCWGG 1 cut(s) 539
BseCI ATCGAT 2 cut(s) 266, 429
BseDI CCNNGG 3 cut(s) 88, 337, 538
BseGI GGATG 7 cut(s) 84, 127, 312, 538, 762, 805, 889
BseLI CCNNNNNNNGG 1 cut(s) 859
BseMII CTCAG 4 cut(s) 32, 657, 884, 1014
BseNI ACTGG 6 cut(s) 694, 804, 844, 980, 1009, 1056
BseRI GAGGAG 1 cut(s) 722
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 3 cut(s) 503, 568, 682
BsgI GTGCAG 1 cut(s) 741
Bsh1236I CGCG 1 cut(s) 1076
BshFI GGCC 2 cut(s) 292, 605
BshNI GGYRCC 2 cut(s) 80, 1034
BshTI ACCGGT 1 cut(s) 176
BshVI ATCGAT 2 cut(s) 266, 429
BsiHKAI GWGCWC 1 cut(s) 776
BsiHKCI CYCGRG 1 cut(s) 1091
BsiSI CCGG 3 cut(s) 78, 165, 177
BslFI GGGAC 2 cut(s) 201, 832
BslI CCNNNNNNNGG 1 cut(s) 859
BsmAI GTCTC 2 cut(s) 835, 1023
BsmBI CGTCTC 1 cut(s) 1023
BsmFI GGGAC 2 cut(s) 201, 832
BsnI GGCC 2 cut(s) 292, 605
BsoBI CYCGRG 1 cut(s) 1091
Bsp119I TTCGAA 1 cut(s) 522
Bsp1286I GDGCHC 3 cut(s) 336, 689, 776
Bsp143I GATC 3 cut(s) 343, 559, 954
BspACI CCGC 4 cut(s) 286, 550, 897, 1076
BspANI GGCC 2 cut(s) 292, 605
BspCNI CTCAG 4 cut(s) 33, 656, 885, 1015
BspDI ATCGAT 2 cut(s) 266, 429
BspFNI CGCG 1 cut(s) 1076
BspLI GGNNCC 5 cut(s) 82, 169, 291, 335, 1036
BspMAI CTGCAG 1 cut(s) 493
BspT104I TTCGAA 1 cut(s) 522
BspT107I GGYRCC 2 cut(s) 80, 1034
BsrBI CCGCTC 1 cut(s) 897
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 6 cut(s) 694, 804, 844, 980, 1009, 1056
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 3 cut(s) 88, 337, 538
BssMI GATC 3 cut(s) 343, 559, 954
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 4 cut(s) 37, 215, 719, 1034
Bst6I CTCTTC 1 cut(s) 845
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 522
BstC8I GCNNGC 2 cut(s) 668, 1074
BstDEI CTNAG 5 cut(s) 41, 105, 643, 893, 1023
BstF5I GGATG 7 cut(s) 84, 127, 312, 538, 762, 805, 889
BstFNI CGCG 1 cut(s) 1076
BstH2I RGCGCY 1 cut(s) 696
BstHHI GCGC 2 cut(s) 318, 695
BstKTI GATC 3 cut(s) 346, 562, 957
BstMAI GTCTC 2 cut(s) 835, 1023
BstMBI GATC 3 cut(s) 343, 559, 954
BstMWI GCNNNNNNNGC 4 cut(s) 66, 140, 728, 976
BstNI CCWGG 1 cut(s) 539
BstSCI CCNGG 2 cut(s) 76, 537
BstSFI CTRYAG 1 cut(s) 489
BstSLI GKGCMC 1 cut(s) 689
BstUI CGCG 1 cut(s) 1076
BstV1I GCAGC 3 cut(s) 503, 568, 682
BstXI CCANNNNNNTGG 1 cut(s) 13
Bsu15I ATCGAT 2 cut(s) 266, 429
BsuRI GGCC 2 cut(s) 292, 605
BsuTUI ATCGAT 2 cut(s) 266, 429
BtgZI GCGATG 1 cut(s) 293
BtsCI GGATG 7 cut(s) 84, 127, 312, 538, 762, 805, 889
BtsIMutI CAGTG 7 cut(s) 211, 687, 724, 890, 918, 987, 1049
Cac8I GCNNGC 2 cut(s) 668, 1074
CfoI GCGC 2 cut(s) 318, 695
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 290
ClaI ATCGAT 2 cut(s) 266, 429
Csp6I GTAC 4 cut(s) 483, 715, 1012, 1035
CspAI ACCGGT 1 cut(s) 176
CviAII CATG 2 cut(s) 827, 854
CviQI GTAC 4 cut(s) 483, 715, 1012, 1035
DdeI CTNAG 5 cut(s) 41, 105, 643, 893, 1023
DpnI GATC 3 cut(s) 345, 561, 956
DpnII GATC 3 cut(s) 343, 559, 954
DrdI GACNNNNNNGTC 1 cut(s) 827
DseDI GACNNNNNNGTC 1 cut(s) 827
EaeI YGGCCR 1 cut(s) 603
Eam1104I CTCTTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 845
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 336
Eco32I GATATC 1 cut(s) 394
Eco57I CTGAAG 3 cut(s) 95, 389, 992
Eco88I CYCGRG 1 cut(s) 1091
EcoRI GAATTC 1 cut(s) 876
EcoRII CCWGG 1 cut(s) 537
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 337
Esp3I CGTCTC 1 cut(s) 1023
FaeI CATG 2 cut(s) 830, 857
FaiI YATR 8 cut(s) 71, 158, 499, 608, 617, 815, 828, 855
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 2 cut(s) 201, 832
FatI CATG 2 cut(s) 826, 853
FauI CCCGC 1 cut(s) 279
Fnu4HI GCNGC 3 cut(s) 492, 582, 696
FokI GGATG 7 cut(s) 71, 134, 319, 545, 769, 812, 896
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 3 cut(s) 492, 582, 696
GlaI GCGC 2 cut(s) 317, 694
GluI GCNGC 3 cut(s) 492, 582, 696
HaeII RGCGCY 1 cut(s) 696
HaeIII GGCC 2 cut(s) 292, 605
HapII CCGG 3 cut(s) 78, 165, 177
HhaI GCGC 2 cut(s) 318, 695
Hin1II CATG 2 cut(s) 830, 857
Hin6I GCGC 2 cut(s) 316, 693
HinP1I GCGC 2 cut(s) 316, 693
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 2 cut(s) 107, 461
HinfI GANTC 5 cut(s) 101, 661, 905, 934, 1019
HpaII CCGG 3 cut(s) 78, 165, 177
HphI GGTGA 3 cut(s) 43, 353, 760
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 8 cut(s) 42, 243, 469, 646, 811, 939, 959, 1024
Hpy188III TCNNGA 4 cut(s) 98, 113, 881, 998
Hpy8I GTNNAC 1 cut(s) 820
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 4 cut(s) 37, 215, 719, 1034
HpyCH4V TGCA 9 cut(s) 134, 143, 434, 477, 491, 584, 670, 722, 923
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 140, 728, 976
HpyF3I CTNAG 5 cut(s) 41, 105, 643, 893, 1023
Hsp92II CATG 2 cut(s) 830, 857
HspAI GCGC 2 cut(s) 316, 693
KpnI GGTACC 1 cut(s) 1038
Kzo9I GATC 3 cut(s) 343, 559, 954
LmnI GCTCC 4 cut(s) 125, 339, 499, 771
Lsp1109I GCAGC 3 cut(s) 503, 568, 682
LweI GCATC 4 cut(s) 421, 486, 740, 790
MaeIII GTNAC 1 cut(s) 31
MalI GATC 3 cut(s) 345, 561, 956
MbiI CCGCTC 1 cut(s) 897
MboI GATC 3 cut(s) 343, 559, 954
MboII GAAGA 7 cut(s) 250, 463, 646, 832, 943, 985, 1055
MhlI GDGCHC 3 cut(s) 336, 689, 776
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 8 cut(s) 72, 138, 442, 554, 751, 865, 876, 1102
MluNI TGGCCA 1 cut(s) 605
MmeI TCCRAC 1 cut(s) 221
Mox20I TGGCCA 1 cut(s) 605
MroXI GAANNNNTTC 1 cut(s) 254
MscI TGGCCA 1 cut(s) 605
MseI TTAA 2 cut(s) 365, 762
MslI CAYNNNNRTG 1 cut(s) 736
Msp20I TGGCCA 1 cut(s) 605
MspI CCGG 3 cut(s) 78, 165, 177
MspR9I CCNGG 2 cut(s) 78, 539
MvaI CCWGG 1 cut(s) 539
MvnI CGCG 1 cut(s) 1076
MwoI GCNNNNNNNGC 4 cut(s) 66, 140, 728, 976
NciI CCSGG 1 cut(s) 78
NdeII GATC 3 cut(s) 343, 559, 954
NlaIII CATG 2 cut(s) 830, 857
NlaIV GGNNCC 5 cut(s) 82, 169, 291, 335, 1036
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 31
NspV TTCGAA 1 cut(s) 522
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 1038
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 5 cut(s) 101, 661, 905, 934, 1019
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 3 cut(s) 493, 583, 697
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 5 cut(s) 82, 169, 291, 335, 1036
PspPI GGNCC 1 cut(s) 290
PstI CTGCAG 1 cut(s) 493
RsaI GTAC 4 cut(s) 484, 716, 1013, 1036
RsaNI GTAC 4 cut(s) 483, 715, 1012, 1035
RseI CAYNNNNRTG 1 cut(s) 736
SaqAI TTAA 2 cut(s) 365, 762
SatI GCNGC 3 cut(s) 492, 582, 696
Sau3AI GATC 3 cut(s) 343, 559, 954
Sau96I GGNCC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 78, 539
SduI GDGCHC 3 cut(s) 336, 689, 776
SfaNI GCATC 4 cut(s) 421, 486, 740, 790
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 522
SgrAI CRCCGGYG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 736
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 8 cut(s) 72, 138, 442, 554, 751, 865, 876, 1102
SsiI CCGC 4 cut(s) 286, 550, 897, 1076
StyD4I CCNGG 2 cut(s) 76, 537
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 4 cut(s) 37, 215, 719, 1034
TaqI TCGA 8 cut(s) 99, 266, 429, 522, 567, 726, 999, 1056
TasI AATT 8 cut(s) 72, 138, 442, 554, 751, 865, 876, 1102
TatI WGTACW 1 cut(s) 714
TfiI GAWTC 5 cut(s) 101, 661, 905, 934, 1019
Tru1I TTAA 2 cut(s) 365, 762
Tru9I TTAA 2 cut(s) 365, 762
TscAI CASTG 7 cut(s) 218, 694, 724, 897, 918, 987, 1056
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 491, 581, 695
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 296, 435, 815, 918
TspRI CASTG 7 cut(s) 218, 694, 724, 897, 918, 987, 1056
XapI RAATTY 3 cut(s) 442, 554, 876
XcmI CCANNNNNNNNNTGG 1 cut(s) 811
XmnI GAANNNNTTC 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.