pycom02g13520

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001289
Physical Location & Seq
Reverse (-)
10023530 .. 10025676
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13520.7

Sequence Viewer

Length: 1341 bp
ATGGCTGCTACCAGTAAGAAGAGTGCTTGGGTTCTTCTTCCTTCACAATCACAATTGCTCATATTATATCTAAGCGACAGAGAAGGTCTGATTTTTCACAAAAATATTTCGATTTCGACAAGATCTTTAATTCGAGTTATAACAATGTCGACTGGTTTGACAAAGAGAAGGATGGTGACAGTACTAAGCATTGATGGTGGTGGCATTAGAGGCATAATTCCCAGCACCCTCCTCGCCTTTCTTGAATCCAAGCTTCAGGAATTGGATGGGCCTGCTGCAAGAATCGCAGATTATTTTGACATAATTGCCGGAACAAGCACCGGTGGTCTTGTCACCACCATGCTTGCAGCTCCTAACAAAGACAACCGACCCATGTACGAAGCAAAGGACATCAACAACTTCTATTTGGATCACACCCCAAAGATTTTCCCTCAGAATAGTCGTAATAACTTCTTGACATCACTAACAAGTATGGTTGGTGCTGTCATGGGACCAAAATATGATGGAAAGTACCTGCGAACATTGACGAAGGAGCTGCTTGGCGACCTCACTCTTAAAGAAACCCTAACCACTGTGATTATTCCAACTTTTGATATTAAGCACCTTCAGCCTGTGCTCTTCTCAACCATCGATGTATTCTTATATAGTTTTAAATCCATGGCACAGGCAAAAGAAAGTCCATTGAAAAATGCTAAGCTTTCGGATATCTGCATCAGTACCTCTGCTGCTCCCACTTTTCTCCCCGCTCATTACTTCGAGGTCAATGATAACGAGGGAAGAACCCGAACTTTTGATCTCATCGATGGTGGGGTTGCTGCCAACAATCCTACGATGACGGCCATAAGCCACATAAACAGAGAGATATTGAAGCATAACTCAGAGCCGATGGATGCTACCAGGTTGCTAGTGCTATCATTGGGCACAGGCGCAGCCAAGAATGAAGCGAAGTACAATGCAGCCAACGCCTCCAAATGGGGTTTGATCAATTGGGTCTTTGACAATGGGAGCACACCTTTGGTAGACATTTTCGGGGATGCAAGTTCCGACATGGTTGATTTTCATGTCTCCACCCTCTTCCAATCCGTTCACGCCAAGGACAACTATCTTCGTATTCAGGATGACACGTTGATTGGTGAGGAGGCATCAGTGGACATTGCAACGGAGAAAAATCTGAAAAGGCTTGGTGAAATCGGAAAGGTGTTGTTGAAGAAGCCGGTGTCACGGGTTAATTTGGATACCGGAAGGTATGAGGAATCCAAGGGAGAAGGTACCTATGAAGAAGCACTGATTGAGTTTGCCAAAAGGCTCTCGGAAGGAAAGAAGCTTAGGCAAAACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

447

Amino Acids

49.16

Weight (kDa)

7.76

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 522
Acc65I GGTACC 1 cut(s) 1268
AccB1I GGYRCC 1 cut(s) 1268
AccBSI CCGCTC 1 cut(s) 746
AccI GTMKAC 2 cut(s) 149, 1020
AciI CCGC 1 cut(s) 744
AclWI GGATC 1 cut(s) 417
AcoI YGGCCR 1 cut(s) 837
AcuI CTGAAG 2 cut(s) 239, 590
AfaI GTAC 6 cut(s) 183, 377, 512, 718, 950, 1270
AfiI CCNNNNNNNGG 1 cut(s) 972
AflIII ACRYGT 1 cut(s) 1122
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 4 cut(s) 245, 685, 868, 1207
AjnI CCWGG 1 cut(s) 896
AluBI AGCT 5 cut(s) 253, 350, 535, 697, 1324
AluI AGCT 5 cut(s) 253, 350, 535, 697, 1324
Alw21I GWGCWC 2 cut(s) 618, 1010
Alw26I GTCTC 1 cut(s) 1069
AlwI GGATC 1 cut(s) 417
AoxI GGCC 2 cut(s) 269, 837
ApeKI GCWGC 8 cut(s) 5, 275, 347, 535, 725, 815, 929, 956
AsiGI ACCGGT 1 cut(s) 320
Asp718I GGTACC 1 cut(s) 1268
AspLEI GCGC 1 cut(s) 929
AspS9I GGNCC 2 cut(s) 269, 491
AsuHPI GGTGA 4 cut(s) 187, 325, 1145, 1196
AvaII GGWCC 1 cut(s) 491
BaeGI GKGCMC 1 cut(s) 923
BanI GGYRCC 1 cut(s) 1268
Bbv12I GWGCWC 2 cut(s) 618, 1010
BbvI GCAGC 7 cut(s) 262, 359, 522, 712, 802, 941, 968
BccI CCATC 7 cut(s) 166, 188, 260, 497, 635, 797, 880
BceAI ACGGC 1 cut(s) 852
BcgI CGANNNNNNTGC 4 cut(s) 214, 248, 517, 551
BciT130I CCWGG 1 cut(s) 898
BciVI GTATCC 1 cut(s) 1228
BclI TGATCA 1 cut(s) 981
BcoDI GTCTC 1 cut(s) 1069
BfaI CTAG 1 cut(s) 905
BfuAI ACCTGC 1 cut(s) 522
BfuI GTATCC 1 cut(s) 1228
BglII AGATCT 1 cut(s) 122
BisI GCNGC 8 cut(s) 6, 276, 348, 536, 726, 816, 930, 957
BlpI GCTNAGC 1 cut(s) 693
BlsI GCNGC 8 cut(s) 7, 277, 349, 537, 727, 817, 931, 958
BmcAI AGTACT 1 cut(s) 183
Bme1390I CCNGG 1 cut(s) 898
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 2 cut(s) 269, 491
BmiI GGNNCC 2 cut(s) 492, 1270
BmrFI CCNGG 1 cut(s) 898
BmsI GCATC 4 cut(s) 720, 880, 1024, 1151
Bpu10I CCTNAGC 1 cut(s) 1325
Bpu1102I GCTNAGC 1 cut(s) 693
Bsa29I ATCGAT 2 cut(s) 630, 801
BsaJI CCNNGG 3 cut(s) 657, 1092, 1257
BsaWI WCCGGW 2 cut(s) 320, 1238
Bsc4I CCNNNNNNNGG 1 cut(s) 972
Bse118I RCCGGY 2 cut(s) 320, 1213
Bse1I ACTGG 2 cut(s) 12, 157
Bse3DI GCAATG 1 cut(s) 1152
BseBI CCWGG 1 cut(s) 898
BseCI ATCGAT 2 cut(s) 630, 801
BseDI CCNNGG 3 cut(s) 657, 1092, 1257
BseGI GGATG 5 cut(s) 177, 271, 895, 1039, 1123
BseLI CCNNNNNNNGG 1 cut(s) 972
BseMI GCAATG 1 cut(s) 1152
BseMII CTCAG 2 cut(s) 446, 891
BseNI ACTGG 2 cut(s) 12, 157
BseRI GAGGAG 2 cut(s) 221, 1151
BseSI GKGCMC 1 cut(s) 923
BseXI GCAGC 7 cut(s) 262, 359, 522, 712, 802, 941, 968
BseYI CCCAGC 1 cut(s) 221
BshFI GGCC 2 cut(s) 271, 839
BshNI GGYRCC 1 cut(s) 1268
BshTI ACCGGT 1 cut(s) 320
BshVI ATCGAT 2 cut(s) 630, 801
BsiHKAI GWGCWC 2 cut(s) 618, 1010
BsiSI CCGG 4 cut(s) 309, 321, 1214, 1239
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 972
BsmAI GTCTC 1 cut(s) 1069
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 2 cut(s) 271, 839
Bsp1286I GDGCHC 3 cut(s) 618, 923, 1010
Bsp143I GATC 4 cut(s) 122, 409, 793, 981
Bsp1720I GCTNAGC 1 cut(s) 693
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 1 cut(s) 744
BspANI GGCC 2 cut(s) 271, 839
BspCNI CTCAG 2 cut(s) 445, 890
BspDI ATCGAT 2 cut(s) 630, 801
BspLI GGNNCC 2 cut(s) 492, 1270
BspMI ACCTGC 1 cut(s) 522
BspPI GGATC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 623
BspT107I GGYRCC 1 cut(s) 1268
BsrBI CCGCTC 1 cut(s) 746
BsrDI GCAATG 1 cut(s) 1152
BsrFI RCCGGY 2 cut(s) 320, 1213
BsrI ACTGG 2 cut(s) 12, 157
BssAI RCCGGY 2 cut(s) 320, 1213
BssECI CCNNGG 3 cut(s) 657, 1092, 1257
BssMI GATC 4 cut(s) 122, 409, 793, 981
BssT1I CCWWGG 3 cut(s) 657, 1092, 1257
Bst2UI CCWGG 1 cut(s) 898
Bst4CI ACNGT 3 cut(s) 181, 574, 1337
Bst6I CTCTTC 3 cut(s) 14, 623, 1079
BstC8I GCNNGC 2 cut(s) 273, 345
BstDEI CTNAG 6 cut(s) 71, 185, 432, 693, 877, 1325
BstDSI CCRYGG 1 cut(s) 657
BstF5I GGATG 5 cut(s) 177, 271, 895, 1039, 1123
BstHHI GCGC 1 cut(s) 929
BstKTI GATC 4 cut(s) 125, 412, 796, 984
BstMAI GTCTC 1 cut(s) 1069
BstMBI GATC 4 cut(s) 122, 409, 793, 981
BstMWI GCNNNNNNNGC 4 cut(s) 210, 284, 607, 962
BstNI CCWGG 1 cut(s) 898
BstSCI CCNGG 1 cut(s) 896
BstSLI GKGCMC 1 cut(s) 923
BstV1I GCAGC 7 cut(s) 262, 359, 522, 712, 802, 941, 968
BstX2I RGATCY 1 cut(s) 122
BstYI RGATCY 1 cut(s) 122
Bsu15I ATCGAT 2 cut(s) 630, 801
BsuI GTATCC 1 cut(s) 1228
BsuRI GGCC 2 cut(s) 271, 839
BsuTUI ATCGAT 2 cut(s) 630, 801
BtgI CCRYGG 1 cut(s) 657
BtsCI GGATG 5 cut(s) 177, 271, 895, 1039, 1123
BtsIMutI CAGTG 3 cut(s) 570, 1152, 1283
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 2 cut(s) 273, 345
CfoI GCGC 1 cut(s) 929
Cfr10I RCCGGY 2 cut(s) 320, 1213
Cfr13I GGNCC 2 cut(s) 269, 491
ClaI ATCGAT 2 cut(s) 630, 801
CsiI ACCWGGT 1 cut(s) 896
Csp6I GTAC 6 cut(s) 182, 376, 511, 717, 949, 1269
CspAI ACCGGT 1 cut(s) 320
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviAII CATG 6 cut(s) 340, 373, 487, 658, 1048, 1061
CviQI GTAC 6 cut(s) 182, 376, 511, 717, 949, 1269
DdeI CTNAG 6 cut(s) 71, 185, 432, 693, 877, 1325
DpnI GATC 4 cut(s) 124, 411, 795, 983
DpnII GATC 4 cut(s) 122, 409, 793, 981
DraI TTTAAA 1 cut(s) 652
EaeI YGGCCR 1 cut(s) 837
Eam1104I CTCTTC 3 cut(s) 14, 623, 1079
EarI CTCTTC 3 cut(s) 14, 623, 1079
Eco130I CCWWGG 3 cut(s) 657, 1092, 1257
Eco32I GATATC 1 cut(s) 706
Eco47I GGWCC 1 cut(s) 491
Eco57I CTGAAG 2 cut(s) 239, 590
EcoRII CCWGG 1 cut(s) 896
EcoRV GATATC 1 cut(s) 706
EcoT14I CCWWGG 3 cut(s) 657, 1092, 1257
ErhI CCWWGG 3 cut(s) 657, 1092, 1257
FaeI CATG 6 cut(s) 343, 376, 490, 661, 1051, 1064
FaqI GGGAC 1 cut(s) 504
FatI CATG 6 cut(s) 339, 372, 486, 657, 1047, 1060
FauI CCCGC 1 cut(s) 751
FbaI TGATCA 1 cut(s) 981
FblI GTMKAC 2 cut(s) 149, 1020
Fnu4HI GCNGC 8 cut(s) 6, 276, 348, 536, 726, 816, 930, 957
FokI GGATG 5 cut(s) 184, 278, 902, 1046, 1130
Fsp4HI GCNGC 8 cut(s) 6, 276, 348, 536, 726, 816, 930, 957
FspBI CTAG 1 cut(s) 905
GlaI GCGC 1 cut(s) 928
GluI GCNGC 8 cut(s) 6, 276, 348, 536, 726, 816, 930, 957
GsaI CCCAGC 1 cut(s) 225
HaeIII GGCC 2 cut(s) 271, 839
HapII CCGG 4 cut(s) 309, 321, 1214, 1239
HhaI GCGC 1 cut(s) 929
Hin1II CATG 6 cut(s) 343, 376, 490, 661, 1051, 1064
Hin6I GCGC 1 cut(s) 927
HinP1I GCGC 1 cut(s) 927
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 3 cut(s) 251, 695, 1322
HinfI GANTC 3 cut(s) 245, 282, 1253
HpaII CCGG 4 cut(s) 309, 321, 1214, 1239
HphI GGTGA 4 cut(s) 187, 325, 1145, 1196
Hpy166II GTNNAC 4 cut(s) 150, 1021, 1087, 1150
Hpy188I TCNGA 8 cut(s) 90, 435, 703, 880, 1045, 1173, 1193, 1312
Hpy188III TCNNGA 4 cut(s) 242, 257, 454, 1115
Hpy8I GTNNAC 4 cut(s) 150, 1021, 1087, 1150
HpyAV CCTTC 8 cut(s) 51, 77, 162, 523, 614, 1236, 1259, 1307
HpyCH4III ACNGT 3 cut(s) 181, 574, 1337
HpyCH4IV ACGT 1 cut(s) 1124
HpyCH4V TGCA 6 cut(s) 278, 347, 711, 956, 1037, 1157
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 284, 607, 962
HpyF3I CTNAG 6 cut(s) 71, 185, 432, 693, 877, 1325
HpySE526I ACGT 1 cut(s) 1124
Hsp92II CATG 6 cut(s) 343, 376, 490, 661, 1051, 1064
HspAI GCGC 1 cut(s) 927
KpnI GGTACC 1 cut(s) 1272
Ksp22I TGATCA 1 cut(s) 981
Kzo9I GATC 4 cut(s) 122, 409, 793, 981
LguI GCTCTTC 1 cut(s) 623
LmnI GCTCC 4 cut(s) 355, 532, 733, 1005
Lsp1109I GCAGC 7 cut(s) 262, 359, 522, 712, 802, 941, 968
LweI GCATC 4 cut(s) 720, 880, 1024, 1151
MabI ACCWGGT 1 cut(s) 896
MaeI CTAG 1 cut(s) 905
MaeII ACGT 1 cut(s) 1124
MaeIII GTNAC 3 cut(s) 175, 331, 1218
MalI GATC 4 cut(s) 124, 411, 795, 983
MbiI CCGCTC 1 cut(s) 746
MboI GATC 4 cut(s) 122, 409, 793, 981
MboII GAAGA 9 cut(s) 26, 29, 31, 610, 789, 1066, 1097, 1219, 1289
MfeI CAATTG 2 cut(s) 53, 985
MflI RGATCY 1 cut(s) 122
MhlI GDGCHC 3 cut(s) 618, 923, 1010
MluCI AATT 7 cut(s) 53, 129, 216, 260, 303, 985, 1228
MmeI TCCRAC 2 cut(s) 608, 1068
MseI TTAA 6 cut(s) 128, 555, 597, 651, 1227, 1339
MslI CAYNNNNRTG 1 cut(s) 338
MspI CCGG 4 cut(s) 309, 321, 1214, 1239
MspR9I CCNGG 1 cut(s) 898
MunI CAATTG 2 cut(s) 53, 985
MvaI CCWGG 1 cut(s) 898
MwoI GCNNNNNNNGC 4 cut(s) 210, 284, 607, 962
NcoI CCATGG 1 cut(s) 657
NdeII GATC 4 cut(s) 122, 409, 793, 981
NlaIII CATG 6 cut(s) 343, 376, 490, 661, 1051, 1064
NlaIV GGNNCC 2 cut(s) 492, 1270
NmuCI GTSAC 3 cut(s) 175, 331, 1218
PciSI GCTCTTC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 245, 282, 1253
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 8 cut(s) 7, 277, 349, 537, 727, 817, 931, 958
PsiI TTATAA 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 896
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 1 cut(s) 896
PspN4I GGNNCC 2 cut(s) 492, 1270
PspPI GGNCC 2 cut(s) 269, 491
PsuI RGATCY 1 cut(s) 122
RsaI GTAC 6 cut(s) 183, 377, 512, 718, 950, 1270
RsaNI GTAC 6 cut(s) 182, 376, 511, 717, 949, 1269
RseI CAYNNNNRTG 1 cut(s) 338
SalI GTCGAC 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 623
SaqAI TTAA 6 cut(s) 128, 555, 597, 651, 1227, 1339
SatI GCNGC 8 cut(s) 6, 276, 348, 536, 726, 816, 930, 957
Sau3AI GATC 4 cut(s) 122, 409, 793, 981
Sau96I GGNCC 2 cut(s) 269, 491
ScaI AGTACT 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 898
SduI GDGCHC 3 cut(s) 618, 923, 1010
SexAI ACCWGGT 1 cut(s) 896
SfaNI GCATC 4 cut(s) 720, 880, 1024, 1151
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 7 cut(s) 53, 129, 216, 260, 303, 985, 1228
SsiI CCGC 1 cut(s) 744
SspI AATATT 1 cut(s) 106
SspMI CTAG 1 cut(s) 905
StyD4I CCNGG 1 cut(s) 896
StyI CCWWGG 3 cut(s) 657, 1092, 1257
TaaI ACNGT 3 cut(s) 181, 574, 1337
TaiI ACGT 1 cut(s) 1127
TaqI TCGA 7 cut(s) 110, 116, 133, 149, 630, 756, 801
TasI AATT 7 cut(s) 53, 129, 216, 260, 303, 985, 1228
TatI WGTACW 2 cut(s) 181, 948
TfiI GAWTC 3 cut(s) 245, 282, 1253
Tru1I TTAA 6 cut(s) 128, 555, 597, 651, 1227, 1339
Tru9I TTAA 6 cut(s) 128, 555, 597, 651, 1227, 1339
TscAI CASTG 3 cut(s) 577, 1152, 1290
TseFI GTSAC 3 cut(s) 175, 331, 1218
TseI GCWGC 8 cut(s) 5, 275, 347, 535, 725, 815, 929, 956
Tsp45I GTSAC 3 cut(s) 175, 331, 1218
TspDTI ATGAA 3 cut(s) 954, 1049, 1290
TspGWI ACGGA 2 cut(s) 1072, 1175
TspRI CASTG 3 cut(s) 577, 1152, 1290
VpaK11BI GGWCC 1 cut(s) 491
XmiI GTMKAC 2 cut(s) 149, 1020
XspI CTAG 1 cut(s) 905
ZrmI AGTACT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.