Rorug02G0123900

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001289
Physical Location & Seq
Forward (+)
10646168 .. 10649893
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0123900.1

Sequence Viewer

Length: 1944 bp
ATGGCCTCCGTCCTCACCGACCGGGTCTTCTTCCAGGGGCGGAAATGGGAATTCACCCTCAACCCGGGGCCGTTCAACGTCAAGGAGCACGTGCTCATCACGATCTTCGCCAACTCCGGCGCCGGCAACGTCTACGCGATCCACATAGTGAGCACCATCAAGGTCTTCTACAAGAAGAACATGTCGTTTTGGGTGGCGCTGCTCGTCGTTTTGACCACTCAGGTGTTGGGGTTCGGCTGGGCCGGGCTTTTCCGCCGGTACTTGGTCGAGCCCGCCGCCATGTGGTGGCCTCATAATCTCGTCCAGGTTTCGCTCTTCAGAGCATTGCATGAGAAGGAGGAGAGGCCAAGAGGTGGATTAACAAGGAATCAGTTCTTCCTCATTGCTTTCACTTGCAGCTTTGCTTATTATGTTCTTCCGGGCTACTTATTCCCAATGTTATCTTCCCTTTCCTGGGTCTGCTGGATCTTCCCTGCTTCAGTGCTAGCCCATCAGCTAGGGTCAGGACTGCATGGTCTTGGGATCGGTGCTATCGGTCTCGATTGGTCCAGCATTTCCTCTTACCTTGGAAGCCCACTTGCCAGTCCATGGTTTGCTACTGCTAATGTTGCTGCTGGTTTTGCCCTTGTGATGTATGTCATTACTCCTGTGGCTTATTGGCTCAATGTGTATAAAGCAAAAACCTTCCCCATATTCTCGGATGGATTGTTCACGTCTACTGGCCAAAGCTACAACATTTCAGCTATAATAGACCCCCACTTCCGGCTTGACATTGATGCATACGAGCGAGAGGGTCCTCTCCATATCAGTACCTTTTTTGCTATCATCTATGGTGTCAATTTTGCTTGCCTTACTGCCACTGTTGTTCATGTTCTCCTCTTCAATGGGAGAGACATCTGGGAGCTAAGCAAGTCTGCCTTCCAAGAGAAGAAGACAGATGTGCACACAAAGCTCATGAGAAGATATAAGCAAGTCCCTGGATGGTGGTTCGTATGTATCCTTGTGGCTAACATAGCCGCGACCATATTTACATGCCATTATTACAATGACCAATTGCAATTGCAATGGTGGGGGGTCTTGCTTGCTTGTGGTCTAGCCTTGTTTTTCACTCTACCTGTTGGAGTCATTTATGCCACAACCAATCAGATGCCAACTTTGAATGTGATCACAGAGTACATAATTGGGTATCTGTATCCGGGATATCCAGCTGCTAACATATTATTCAAAGTATATGGACACATAAGCATGAAACAAGGAATCAATTTTCTCGAAGACTTCAAGCTTGGACACTACATGAAGATTGCTCCTCGAGCAATGTTCATGGCACAGGTAGTTGGTACTATCATAGCAGCACTTGTGCATTTGGGAACTGCATGGTGGCTTATGAGCACAATCCCTGACATATGCGACAGGGCGTTACTACCAGCAGAGAGCCCATGGACTTGCCCCGGTGACCATGTCTTCTACGATGCTTCTGTTATCTGGGGCCTTGTCGGGCCTCGCAGAATCTTTGGGGACCTTGGCTACTATTCGGCTATCAACTGGTTTTTCTTAGCTGGGGCTATAGCTCCTGCCCTTGTTTGGCTTGCACACAAGGCCTTCCCAACCAAACAATGGATTAGACTTATTACAATGCCTGTGCTCTTAGGGACAGTGGTCAACATGCCTCCAGCTACTGCTGTGAATTTCACTAGCTGGATCCTCATTGGATTCGCCTCAGGCTTTGTTGCTTACAGGTACTACCGCGGCTGGTGGAGCCGCCATAACTATGTGCTATCTGGAGCTCTGGATGCTGGATTAGCCTTTATGGCGGTAATTTTGTACTTGTGTCTAGGGATGCAACATGTCCACTTAAAATGGTGGGGAAGTACGCCGGATGGTTGCCCGTTGGCTTCTTGCTCAACGGAGCCAGGTGTTGTGATTAATGGCTGTCCATTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

647

Amino Acids

72.52

Weight (kDa)

8.9

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 14 - 610 2.8e-158 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 513
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 4 cut(s) 285, 353, 588, 1614
AccI GTMKAC 2 cut(s) 132, 716
AccII CGCG 3 cut(s) 137, 1019, 1746
AciI CCGC 9 cut(s) 40, 253, 273, 276, 1017, 1744, 1746, 1759, 1811
AclWI GGATC 5 cut(s) 133, 473, 530, 1693, 1706
AcoI YGGCCR 1 cut(s) 721
AcsI RAATTY 2 cut(s) 50, 1684
AcuI CTGAAG 2 cut(s) 301, 462
AcvI CACGTG 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 120
AdeI CACNNNGTG 1 cut(s) 148
AfaI GTAC 7 cut(s) 260, 811, 1175, 1339, 1739, 1823, 1870
AflIII ACRYGT 2 cut(s) 180, 1843
AgsI TTSAA 5 cut(s) 76, 883, 1159, 1225, 1279
AjiI CACGTC 1 cut(s) 714
AjnI CCWGG 5 cut(s) 33, 303, 452, 976, 1909
AjuI GAANNNNNNNTTGG 2 cut(s) 427, 459
AleI CACNNNNGTG 1 cut(s) 221
AloI GAACNNNNNNTCC 2 cut(s) 692, 724
Alw21I GWGCWC 7 cut(s) 90, 96, 155, 945, 1391, 1644, 1786
Alw26I GTCTC 2 cut(s) 542, 885
Alw44I GTGCAC 1 cut(s) 941
AlwI GGATC 5 cut(s) 133, 473, 530, 1693, 1706
AlwNI CAGNNNCTG 1 cut(s) 1676
Ama87I CYCGRG 2 cut(s) 64, 1308
AoxI GGCC 9 cut(s) 3, 68, 240, 287, 344, 721, 1486, 1496, 1596
ApaLI GTGCAC 1 cut(s) 941
ApeKI GCWGC 5 cut(s) 199, 396, 611, 1208, 1349
ApoI RAATTY 2 cut(s) 50, 1684
AseI ATTAAT 1 cut(s) 1923
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 2 cut(s) 122, 199
AspS9I GGNCC 7 cut(s) 68, 240, 546, 794, 1486, 1496, 1516
AsuC2I CCSGG 7 cut(s) 23, 65, 66, 244, 420, 1197, 1449
AsuHPI GGTGA 3 cut(s) 7, 46, 1463
AsuNHI GCTAGC 1 cut(s) 484
AvaI CYCGRG 2 cut(s) 64, 1308
AvaII GGWCC 3 cut(s) 546, 794, 1516
AxyI CCTNAGG 1 cut(s) 1717
BaeGI GKGCMC 1 cut(s) 945
BalI TGGCCA 1 cut(s) 723
BamHI GGATCC 1 cut(s) 1698
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 3 cut(s) 273, 1436, 1786
BbrPI CACGTG 1 cut(s) 91
BbsI GAAGAC 5 cut(s) 19, 157, 938, 1278, 1453
Bbv12I GWGCWC 7 cut(s) 90, 96, 155, 945, 1391, 1644, 1786
BbvI GCAGC 5 cut(s) 186, 408, 598, 1195, 1361
BccI CCATC 5 cut(s) 164, 498, 695, 975, 1871
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 5 cut(s) 35, 305, 454, 978, 1911
BciVI GTATCC 2 cut(s) 1007, 1203
BclI TGATCA 1 cut(s) 1164
BcnI CCSGG 7 cut(s) 23, 65, 66, 244, 420, 1197, 1449
BcoDI GTCTC 2 cut(s) 542, 885
BfaI CTAG 5 cut(s) 485, 497, 1094, 1692, 1832
BfmI CTRYAG 1 cut(s) 1563
BfoI RGCGCY 2 cut(s) 123, 200
BfuI GTATCC 2 cut(s) 1007, 1203
BglI GCCNNNNNGGC 1 cut(s) 1808
BisI GCNGC 9 cut(s) 200, 276, 397, 612, 1017, 1209, 1350, 1747, 1759
BlpI GCTNAGC 1 cut(s) 905
BlsI GCNGC 9 cut(s) 201, 277, 398, 613, 1018, 1210, 1351, 1748, 1760
Bme18I GGWCC 3 cut(s) 546, 794, 1516
BmeT110I CYCGRG 2 cut(s) 64, 1308
BmgBI CACGTC 1 cut(s) 714
BmgT120I GGNCC 7 cut(s) 68, 240, 546, 794, 1486, 1496, 1516
BmiI GGNNCC 8 cut(s) 69, 121, 795, 1487, 1517, 1700, 1757, 1908
BmsI GCATC 5 cut(s) 766, 1137, 1459, 1780, 1827
BmtI GCTAGC 1 cut(s) 488
BoxI GACNNNNGTC 1 cut(s) 1655
BpiI GAAGAC 5 cut(s) 19, 157, 938, 1278, 1453
BpmI CTGGAG 2 cut(s) 1653, 1800
Bpu1102I GCTNAGC 1 cut(s) 905
BpuMI CCSGG 7 cut(s) 23, 65, 66, 244, 420, 1197, 1449
BsaAI YACGTR 1 cut(s) 91
BsaHI GRCGYC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 542
Bse118I RCCGGY 2 cut(s) 122, 255
Bse1I ACTGG 3 cut(s) 582, 724, 1547
Bse21I CCTNAGG 1 cut(s) 1717
Bse3DI GCAATG 4 cut(s) 323, 381, 1070, 1320
BseBI CCWGG 5 cut(s) 35, 305, 454, 978, 1911
BseGI GGATG 5 cut(s) 706, 986, 1795, 1842, 1882
BseMI GCAATG 4 cut(s) 323, 381, 1070, 1320
BseMII CTCAG 2 cut(s) 233, 1731
BseNI ACTGG 3 cut(s) 582, 724, 1547
BseRI GAGGAG 3 cut(s) 353, 866, 1296
BseSI GKGCMC 1 cut(s) 945
BseXI GCAGC 5 cut(s) 186, 408, 598, 1195, 1361
BseYI CCCAGC 2 cut(s) 237, 1556
Bsh1236I CGCG 3 cut(s) 137, 1019, 1746
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 9 cut(s) 5, 70, 242, 289, 346, 723, 1488, 1498, 1598
BshNI GGYRCC 1 cut(s) 119
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 7 cut(s) 90, 96, 155, 945, 1391, 1644, 1786
BsiHKCI CYCGRG 2 cut(s) 64, 1308
BslFI GGGAC 3 cut(s) 959, 1529, 1663
BsmAI GTCTC 2 cut(s) 542, 885
BsmFI GGGAC 3 cut(s) 959, 1529, 1663
BsnI GGCC 9 cut(s) 5, 70, 242, 289, 346, 723, 1488, 1498, 1598
Bso31I GGTCTC 1 cut(s) 542
BsoBI CYCGRG 2 cut(s) 64, 1308
Bsp1286I GDGCHC 9 cut(s) 90, 96, 155, 273, 945, 1391, 1436, 1644, 1786
Bsp143I GATC 6 cut(s) 102, 138, 465, 522, 1164, 1698
Bsp1720I GCTNAGC 1 cut(s) 905
Bsp19I CCATGG 2 cut(s) 587, 1436
BspACI CCGC 9 cut(s) 40, 253, 273, 276, 1017, 1744, 1746, 1759, 1811
BspANI GGCC 9 cut(s) 5, 70, 242, 289, 346, 723, 1488, 1498, 1598
BspCNI CTCAG 2 cut(s) 232, 1730
BspFNI CGCG 3 cut(s) 137, 1019, 1746
BspHI TCATGA 1 cut(s) 954
BspLI GGNNCC 8 cut(s) 69, 121, 795, 1487, 1517, 1700, 1757, 1908
BspOI GCTAGC 1 cut(s) 488
BspPI GGATC 5 cut(s) 133, 473, 530, 1693, 1706
BspQI GCTCTTC 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 542
BsrDI GCAATG 4 cut(s) 323, 381, 1070, 1320
BsrFI RCCGGY 2 cut(s) 122, 255
BsrI ACTGG 3 cut(s) 582, 724, 1547
BssAI RCCGGY 2 cut(s) 122, 255
BssMI GATC 6 cut(s) 102, 138, 465, 522, 1164, 1698
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 4 cut(s) 565, 587, 1436, 1519
Bst2UI CCWGG 5 cut(s) 35, 305, 454, 978, 1911
Bst4CI ACNGT 2 cut(s) 862, 1654
Bst6I CTCTTC 2 cut(s) 320, 884
BstACI GRCGYC 1 cut(s) 120
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 6 cut(s) 124, 273, 486, 847, 1083, 1587
BstDEI CTNAG 5 cut(s) 219, 905, 1552, 1645, 1717
BstDSI CCRYGG 3 cut(s) 587, 1436, 1744
BstEII GGTNACC 1 cut(s) 1451
BstF5I GGATG 5 cut(s) 706, 986, 1795, 1842, 1882
BstFNI CGCG 3 cut(s) 137, 1019, 1746
BstH2I RGCGCY 2 cut(s) 123, 200
BstHHI GCGC 2 cut(s) 122, 199
BstKTI GATC 6 cut(s) 105, 141, 468, 525, 1167, 1701
BstMAI GTCTC 2 cut(s) 542, 885
BstMBI GATC 6 cut(s) 102, 138, 465, 522, 1164, 1698
BstMCI CGRYCG 1 cut(s) 22
BstNI CCWGG 5 cut(s) 35, 305, 454, 978, 1911
BstNSI RCATGY 4 cut(s) 184, 1035, 1666, 1847
BstPAI GACNNNNGTC 1 cut(s) 1655
BstPI GGTNACC 1 cut(s) 1451
BstSFI CTRYAG 1 cut(s) 1563
BstSLI GKGCMC 1 cut(s) 945
BstUI CGCG 3 cut(s) 137, 1019, 1746
BstV1I GCAGC 5 cut(s) 186, 408, 598, 1195, 1361
BstV2I GAAGAC 5 cut(s) 19, 157, 938, 1278, 1453
BstX2I RGATCY 2 cut(s) 465, 1698
BstYI RGATCY 2 cut(s) 465, 1698
Bsu36I CCTNAGG 1 cut(s) 1717
BsuI GTATCC 2 cut(s) 1007, 1203
BsuRI GGCC 9 cut(s) 5, 70, 242, 289, 346, 723, 1488, 1498, 1598
BtgI CCRYGG 3 cut(s) 587, 1436, 1744
BtrI CACGTC 1 cut(s) 714
BtsCI GGATG 5 cut(s) 706, 986, 1795, 1842, 1882
BtsIMutI CAGTG 3 cut(s) 486, 858, 1659
Cac8I GCNNGC 6 cut(s) 124, 273, 486, 847, 1083, 1587
CaiI CAGNNNCTG 1 cut(s) 1676
CciI TCATGA 1 cut(s) 954
CfoI GCGC 2 cut(s) 122, 199
Cfr10I RCCGGY 2 cut(s) 122, 255
Cfr13I GGNCC 7 cut(s) 68, 240, 546, 794, 1486, 1496, 1516
Cfr42I CCGCGG 1 cut(s) 1747
Cfr9I CCCGGG 1 cut(s) 64
Csp6I GTAC 7 cut(s) 259, 810, 1174, 1338, 1738, 1822, 1869
CviQI GTAC 7 cut(s) 259, 810, 1174, 1338, 1738, 1822, 1869
DdeI CTNAG 5 cut(s) 219, 905, 1552, 1645, 1717
DinI GGCGCC 1 cut(s) 121
DpnI GATC 6 cut(s) 104, 140, 467, 524, 1166, 1700
DpnII GATC 6 cut(s) 102, 138, 465, 522, 1164, 1698
DraIII CACNNNGTG 1 cut(s) 148
DrdI GACNNNNNNGTC 1 cut(s) 513
DseDI GACNNNNNNGTC 1 cut(s) 513
EaeI YGGCCR 1 cut(s) 721
Eam1104I CTCTTC 2 cut(s) 320, 884
EarI CTCTTC 2 cut(s) 320, 884
EciI GGCGGA 2 cut(s) 55, 242
Ecl136II GAGCTC 1 cut(s) 1784
Eco130I CCWWGG 4 cut(s) 565, 587, 1436, 1519
Eco147I AGGCCT 1 cut(s) 1598
Eco24I GRGCYC 3 cut(s) 273, 1436, 1786
Eco31I GGTCTC 1 cut(s) 542
Eco32I GATATC 1 cut(s) 1202
Eco47I GGWCC 3 cut(s) 546, 794, 1516
Eco53kI GAGCTC 1 cut(s) 1784
Eco57I CTGAAG 2 cut(s) 301, 462
Eco72I CACGTG 1 cut(s) 91
Eco81I CCTNAGG 1 cut(s) 1717
Eco88I CYCGRG 2 cut(s) 64, 1308
Eco91I GGTNACC 1 cut(s) 1451
EcoICRI GAGCTC 1 cut(s) 1784
EcoO109I RGGNCCY 3 cut(s) 794, 1486, 1516
EcoO65I GGTNACC 1 cut(s) 1451
EcoRI GAATTC 1 cut(s) 50
EcoRII CCWGG 5 cut(s) 33, 303, 452, 976, 1909
EcoRV GATATC 1 cut(s) 1202
EcoT14I CCWWGG 4 cut(s) 565, 587, 1436, 1519
EcoT22I ATGCAT 1 cut(s) 781
EcoT38I GRGCYC 3 cut(s) 273, 1436, 1786
EgeI GGCGCC 1 cut(s) 121
EheI GGCGCC 1 cut(s) 121
ErhI CCWWGG 4 cut(s) 565, 587, 1436, 1519
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 3 cut(s) 959, 1529, 1663
FauI CCCGC 1 cut(s) 280
FauNDI CATATG 1 cut(s) 1403
FbaI TGATCA 1 cut(s) 1164
FblI GTMKAC 2 cut(s) 132, 716
Fnu4HI GCNGC 9 cut(s) 200, 276, 397, 612, 1017, 1209, 1350, 1747, 1759
FokI GGATG 5 cut(s) 713, 993, 1802, 1849, 1889
FriOI GRGCYC 3 cut(s) 273, 1436, 1786
Fsp4HI GCNGC 9 cut(s) 200, 276, 397, 612, 1017, 1209, 1350, 1747, 1759
FspBI CTAG 5 cut(s) 485, 497, 1094, 1692, 1832
GlaI GCGC 2 cut(s) 121, 198
GluI GCNGC 9 cut(s) 200, 276, 397, 612, 1017, 1209, 1350, 1747, 1759
GsaI CCCAGC 2 cut(s) 241, 1560
GsuI CTGGAG 2 cut(s) 1653, 1800
HaeII RGCGCY 2 cut(s) 123, 200
HaeIII GGCC 9 cut(s) 5, 70, 242, 289, 346, 723, 1488, 1498, 1598
HhaI GCGC 2 cut(s) 122, 199
Hin1I GRCGYC 1 cut(s) 120
Hin6I GCGC 2 cut(s) 120, 197
HinP1I GCGC 2 cut(s) 120, 197
HincII GTYRAC 1 cut(s) 1660
HindII GTYRAC 1 cut(s) 1660
HindIII AAGCTT 1 cut(s) 1280
HinfI GANTC 5 cut(s) 367, 1122, 1257, 1506, 1710
HphI GGTGA 3 cut(s) 7, 46, 1463
Hpy166II GTNNAC 6 cut(s) 133, 711, 717, 943, 1660, 1849
Hpy188I TCNGA 3 cut(s) 320, 700, 1146
Hpy188III TCNNGA 7 cut(s) 100, 504, 539, 955, 1268, 1779, 1787
Hpy8I GTNNAC 6 cut(s) 133, 711, 717, 943, 1660, 1849
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 4 cut(s) 328, 694, 928, 1609
HpyCH4III ACNGT 2 cut(s) 862, 1654
HpyCH4IV ACGT 4 cut(s) 78, 90, 129, 713
HpyF3I CTNAG 5 cut(s) 219, 905, 1552, 1645, 1717
HpySE526I ACGT 4 cut(s) 78, 90, 129, 713
Hsp92I GRCGYC 1 cut(s) 120
HspAI GCGC 2 cut(s) 120, 197
KasI GGCGCC 1 cut(s) 119
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 1164
KspI CCGCGG 1 cut(s) 1747
Kzo9I GATC 6 cut(s) 102, 138, 465, 522, 1164, 1698
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 7 cut(s) 85, 901, 1309, 1573, 1755, 1781, 1906
Lsp1109I GCAGC 5 cut(s) 186, 408, 598, 1195, 1361
LweI GCATC 5 cut(s) 766, 1137, 1459, 1780, 1827
MaeI CTAG 5 cut(s) 485, 497, 1094, 1692, 1832
MaeII ACGT 4 cut(s) 78, 90, 129, 713
MaeIII GTNAC 2 cut(s) 1416, 1451
MalI GATC 6 cut(s) 104, 140, 467, 524, 1166, 1700
MboI GATC 6 cut(s) 102, 138, 465, 522, 1164, 1698
MfeI CAATTG 2 cut(s) 1052, 1058
MflI RGATCY 2 cut(s) 465, 1698
MhlI GDGCHC 9 cut(s) 90, 96, 155, 273, 945, 1391, 1436, 1644, 1786
MlsI TGGCCA 1 cut(s) 723
MluCI AATT 8 cut(s) 50, 838, 1052, 1058, 1179, 1261, 1684, 1815
MluNI TGGCCA 1 cut(s) 723
Mly113I GGCGCC 1 cut(s) 120
MlyI GAGTC 1 cut(s) 1131
MmeI TCCRAC 1 cut(s) 1099
Mox20I TGGCCA 1 cut(s) 723
Mph1103I ATGCAT 1 cut(s) 781
MroNI GCCGGC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 371
MscI TGGCCA 1 cut(s) 723
MseI TTAA 3 cut(s) 359, 1853, 1923
MslI CAYNNNNRTG 4 cut(s) 221, 1244, 1767, 1939
Msp20I TGGCCA 1 cut(s) 723
MspA1I CMGCKG 2 cut(s) 1208, 1746
MunI CAATTG 2 cut(s) 1052, 1058
MvaI CCWGG 5 cut(s) 35, 305, 454, 978, 1911
MvnI CGCG 3 cut(s) 137, 1019, 1746
NaeI GCCGGC 1 cut(s) 124
NarI GGCGCC 1 cut(s) 120
NciI CCSGG 7 cut(s) 23, 65, 66, 244, 420, 1197, 1449
NcoI CCATGG 2 cut(s) 587, 1436
NdeI CATATG 1 cut(s) 1403
NdeII GATC 6 cut(s) 102, 138, 465, 522, 1164, 1698
NgoMIV GCCGGC 1 cut(s) 122
NheI GCTAGC 1 cut(s) 484
NlaIV GGNNCC 8 cut(s) 69, 121, 795, 1487, 1517, 1700, 1757, 1908
NmuCI GTSAC 1 cut(s) 1451
NsiI ATGCAT 1 cut(s) 781
NspI RCATGY 4 cut(s) 184, 1035, 1666, 1847
OliI CACNNNNGTG 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 1308
PagI TCATGA 1 cut(s) 954
PceI AGGCCT 1 cut(s) 1598
PciI ACATGT 2 cut(s) 180, 1843
PciSI GCTCTTC 1 cut(s) 320
PdiI GCCGGC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 4 cut(s) 367, 1257, 1506, 1710
PflFI GACNNNGTC 2 cut(s) 23, 1457
PflMI CCANNNNNTGG 4 cut(s) 285, 353, 588, 1614
PfoI TCCNGGA 1 cut(s) 1195
PkrI GCNGC 9 cut(s) 201, 277, 398, 613, 1018, 1210, 1351, 1748, 1760
PleI GAGTC 1 cut(s) 1130
PluTI GGCGCC 1 cut(s) 123
PmaCI CACGTG 1 cut(s) 91
PmlI CACGTG 1 cut(s) 91
PpsI GAGTC 1 cut(s) 1130
Ppu21I YACGTR 1 cut(s) 91
PpuMI RGGWCCY 2 cut(s) 794, 1516
PscI ACATGT 2 cut(s) 180, 1843
PshAI GACNNNNGTC 1 cut(s) 1655
PshBI ATTAAT 1 cut(s) 1923
Psp124BI GAGCTC 1 cut(s) 1786
Psp5II RGGWCCY 2 cut(s) 794, 1516
Psp6I CCWGG 5 cut(s) 33, 303, 452, 976, 1909
PspCI CACGTG 1 cut(s) 91
PspEI GGTNACC 1 cut(s) 1451
PspFI CCCAGC 2 cut(s) 237, 1556
PspGI CCWGG 5 cut(s) 33, 303, 452, 976, 1909
PspN4I GGNNCC 8 cut(s) 69, 121, 795, 1487, 1517, 1700, 1757, 1908
PspPI GGNCC 7 cut(s) 68, 240, 546, 794, 1486, 1496, 1516
PspPPI RGGWCCY 2 cut(s) 794, 1516
PspXI VCTCGAGB 1 cut(s) 1308
PstNI CAGNNNCTG 1 cut(s) 1676
PsuI RGATCY 2 cut(s) 465, 1698
PsyI GACNNNGTC 2 cut(s) 23, 1457
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 7 cut(s) 260, 811, 1175, 1339, 1739, 1823, 1870
RsaNI GTAC 7 cut(s) 259, 810, 1174, 1338, 1738, 1822, 1869
RseI CAYNNNNRTG 4 cut(s) 221, 1244, 1767, 1939
SacI GAGCTC 1 cut(s) 1786
SacII CCGCGG 1 cut(s) 1747
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 3 cut(s) 359, 1853, 1923
SatI GCNGC 9 cut(s) 200, 276, 397, 612, 1017, 1209, 1350, 1747, 1759
Sau3AI GATC 6 cut(s) 102, 138, 465, 522, 1164, 1698
Sau96I GGNCC 7 cut(s) 68, 240, 546, 794, 1486, 1496, 1516
SchI GAGTC 1 cut(s) 1131
SduI GDGCHC 9 cut(s) 90, 96, 155, 273, 945, 1391, 1436, 1644, 1786
SfaNI GCATC 5 cut(s) 766, 1137, 1459, 1780, 1827
SfcI CTRYAG 1 cut(s) 1563
SfoI GGCGCC 1 cut(s) 121
Sfr274I CTCGAG 1 cut(s) 1308
Sfr303I CCGCGG 1 cut(s) 1747
SgrBI CCGCGG 1 cut(s) 1747
SinI GGWCC 3 cut(s) 546, 794, 1516
SlaI CTCGAG 1 cut(s) 1308
SmaI CCCGGG 1 cut(s) 66
SmiMI CAYNNNNRTG 4 cut(s) 221, 1244, 1767, 1939
SmlI CTYRAG 1 cut(s) 1308
SmoI CTYRAG 1 cut(s) 1308
Sse9I AATT 8 cut(s) 50, 838, 1052, 1058, 1179, 1261, 1684, 1815
SseBI AGGCCT 1 cut(s) 1598
SsiI CCGC 9 cut(s) 40, 253, 273, 276, 1017, 1744, 1746, 1759, 1811
SspDI GGCGCC 1 cut(s) 119
SspMI CTAG 5 cut(s) 485, 497, 1094, 1692, 1832
SstI GAGCTC 1 cut(s) 1786
StuI AGGCCT 1 cut(s) 1598
StyI CCWWGG 4 cut(s) 565, 587, 1436, 1519
TaaI ACNGT 2 cut(s) 862, 1654
TaiI ACGT 4 cut(s) 81, 93, 132, 716
TaqI TCGA 4 cut(s) 267, 540, 1269, 1309
TaqII GACCGA 1 cut(s) 524
TasI AATT 8 cut(s) 50, 838, 1052, 1058, 1179, 1261, 1684, 1815
TatI WGTACW 2 cut(s) 1173, 1821
TauI GCSGC 4 cut(s) 278, 1019, 1749, 1761
TfiI GAWTC 4 cut(s) 367, 1257, 1506, 1710
Tru1I TTAA 3 cut(s) 359, 1853, 1923
Tru9I TTAA 3 cut(s) 359, 1853, 1923
TscAI CASTG 3 cut(s) 486, 865, 1659
TseFI GTSAC 1 cut(s) 1451
TseI GCWGC 5 cut(s) 199, 396, 611, 1208, 1349
Tsp45I GTSAC 1 cut(s) 1451
TspDTI ATGAA 4 cut(s) 857, 1262, 1309, 1310
TspGWI ACGGA 1 cut(s) 1919
TspMI CCCGGG 1 cut(s) 64
TspRI CASTG 3 cut(s) 486, 865, 1659
Tth111I GACNNNGTC 2 cut(s) 23, 1457
Van91I CCANNNNNTGG 4 cut(s) 285, 353, 588, 1614
VneI GTGCAC 1 cut(s) 941
VpaK11BI GGWCC 3 cut(s) 546, 794, 1516
VspI ATTAAT 1 cut(s) 1923
XapI RAATTY 2 cut(s) 50, 1684
XceI RCATGY 4 cut(s) 184, 1035, 1666, 1847
XcmI CCANNNNNNNNNTGG 2 cut(s) 223, 1611
XhoI CTCGAG 1 cut(s) 1308
XmaI CCCGGG 1 cut(s) 64
XmiI GTMKAC 2 cut(s) 132, 716
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 5 cut(s) 485, 497, 1094, 1692, 1832
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.