pycom15g24290

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001289
Physical Location & Seq
Reverse (-)
18495501 .. 18497596
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g24290.5

Sequence Viewer

Length: 1185 bp
ATGGCTGCTACCATTAAGAATAATGTGTGGGTTCTTGCTATAAATAGCATACTTCATCTCCGTATGCTTCACAACAAGCTTCCTTCACAATCACAACTGGCTCACATCAAGTGCCTTACTGATTTTTTTCGAAAAAATTTCAATTTCGACAAGGTCTCGTTAATTCGAGTTATAACAATGACGACTGGTTTGACAAAGAGAAGGACGGTGACAGTACTAAGCATTGATGGAGGTGGCATTAGAGGCATAATTCCCAGCACCCTCCTCGCCTTTCTTGAATCCAAGCTTCAGGAATTGGATGGGCCTGATGCAAGAGTCGCAGATTACTTCGACATAATTGCCGGAACAAGCACCGGTGGTCTTGTCACCACCATGCTTGCAGCTCCCAACAAAGACAACAGACCCATGTTCGAAGCAAAGGATATCAACAACTTCTATTTCGATCACACCCCTAAGATTTTCCCTCAGAATAGTCGTAATAACTTCTTGACATCACTAACAAGTATGGTTGGTGCTGTCATGGGACCAAAATATGATGGAAAGTACCTGCGAACATTGACGAACGAGCTGCTTGGCGATCTCACTCTTAAACAAACCCTAACCACTGTGCTTATTCCAACTTTTGATATTAAGCACCTTCAGCCTGTGATCTTCTCAACCATCGATACGATGACGGCCATAAGCCACATAAACAGAGAGATATTGAAGCATAAATCAGAGCCGATTGATGCGACAAGGTTGCTAGTGCTATCATTGGGCACAGGCGCAGCCAAGCATGAAGAGAAGTATAATGCAGCCACGGCCTCCAACTGGGGTTTGATCAATTGGTTCTTTGACAATGGGAGCACACCTTTGGTAGATATTTTCGGCGATGCAAGTTCCGATATGGTTGACCTTCATGTCTCCACCCTCTTCCAATCCGTCCACGCCAAGGACAACTACCTCCGTATTCAGGATGACACGTTGATCGGCGAGGAGGCATCATTGGACATTGCAACGGAGAAAAATTTGAAAAGGCTTGTTGAGATCGGGAATGCGTTGTTGAAGAAGCCGGTTTCACGTGTGAATTTGGATACCGGAAAGTATGAGGTATCCAAGGAAGAAGGTACCTATGAAGAAGCACTGATTGATTTTGCCAACAGGCTCTCAGAGGGAAAGAAGCTTATAGACAAAACAATTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

395

Amino Acids

43.84

Weight (kDa)

6.81

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 173
AasI GACNNNNNNGTC 1 cut(s) 899
Acc36I ACCTGC 1 cut(s) 555
Acc65I GGTACC 1 cut(s) 1106
AccB1I GGYRCC 1 cut(s) 1106
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 3 cut(s) 136, 1006, 1066
AcuI CTGAAG 2 cut(s) 272, 623
AcvI CACGTG 1 cut(s) 1061
AfaI GTAC 3 cut(s) 216, 545, 1108
AfiI CCNNNNNNNGG 3 cut(s) 810, 931, 952
AflIII ACRYGT 2 cut(s) 960, 1060
AgeI ACCGGT 1 cut(s) 353
AgsI TTSAA 5 cut(s) 142, 278, 706, 1012, 1045
AluBI AGCT 5 cut(s) 79, 286, 383, 568, 1162
AluI AGCT 5 cut(s) 79, 286, 383, 568, 1162
Alw21I GWGCWC 1 cut(s) 848
Alw26I GTCTC 2 cut(s) 160, 907
AoxI GGCC 3 cut(s) 302, 675, 801
ApeKI GCWGC 5 cut(s) 5, 380, 568, 767, 794
ApoI RAATTY 3 cut(s) 136, 1006, 1066
ArsI GACNNNNNNTTYG 2 cut(s) 172, 204
AsiGI ACCGGT 1 cut(s) 353
Asp718I GGTACC 1 cut(s) 1106
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 2 cut(s) 302, 524
AsuHPI GGTGA 2 cut(s) 220, 358
AsuII TTCGAA 2 cut(s) 130, 411
AvaII GGWCC 1 cut(s) 524
BaeGI GKGCMC 1 cut(s) 761
BanI GGYRCC 1 cut(s) 1106
BbrPI CACGTG 1 cut(s) 1061
Bbv12I GWGCWC 1 cut(s) 848
BbvI GCAGC 4 cut(s) 392, 555, 779, 806
BccI CCATC 4 cut(s) 221, 293, 530, 668
BceAI ACGGC 2 cut(s) 690, 816
BcgI CGANNNNNNTGC 8 cut(s) 247, 281, 320, 354, 550, 584, 721, 755
BciVI GTATCC 2 cut(s) 1066, 1102
BclI TGATCA 1 cut(s) 819
BcoDI GTCTC 2 cut(s) 160, 907
BfaI CTAG 1 cut(s) 743
BfuAI ACCTGC 1 cut(s) 555
BfuI GTATCC 2 cut(s) 1066, 1102
BisI GCNGC 5 cut(s) 6, 381, 569, 768, 795
BlsI GCNGC 5 cut(s) 7, 382, 570, 769, 796
BmcAI AGTACT 1 cut(s) 216
Bme18I GGWCC 1 cut(s) 524
BmgT120I GGNCC 2 cut(s) 302, 524
BmiI GGNNCC 2 cut(s) 525, 1108
BmrI ACTGGG 1 cut(s) 820
BmsI GCATC 4 cut(s) 298, 718, 862, 989
BmuI ACTGGG 1 cut(s) 820
Bpu14I TTCGAA 2 cut(s) 130, 411
Bsa29I ATCGAT 1 cut(s) 663
BsaAI YACGTR 1 cut(s) 1061
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 3 cut(s) 798, 930, 1095
BsaWI WCCGGW 2 cut(s) 353, 1076
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 3 cut(s) 810, 931, 952
Bse118I RCCGGY 2 cut(s) 353, 1051
Bse1I ACTGG 3 cut(s) 102, 190, 815
Bse3DI GCAATG 1 cut(s) 990
BseCI ATCGAT 1 cut(s) 663
BseDI CCNNGG 3 cut(s) 798, 930, 1095
BseGI GGATG 2 cut(s) 304, 961
BseLI CCNNNNNNNGG 3 cut(s) 810, 931, 952
BseMI GCAATG 1 cut(s) 990
BseMII CTCAG 2 cut(s) 479, 1161
BseNI ACTGG 3 cut(s) 102, 190, 815
BseRI GAGGAG 2 cut(s) 254, 989
BseSI GKGCMC 1 cut(s) 761
BseXI GCAGC 4 cut(s) 392, 555, 779, 806
BseYI CCCAGC 1 cut(s) 254
BshFI GGCC 3 cut(s) 304, 677, 803
BshNI GGYRCC 1 cut(s) 1106
BshTI ACCGGT 1 cut(s) 353
BshVI ATCGAT 1 cut(s) 663
BsiHKAI GWGCWC 1 cut(s) 848
BsiSI CCGG 4 cut(s) 342, 354, 1052, 1077
BslFI GGGAC 1 cut(s) 537
BslI CCNNNNNNNGG 3 cut(s) 810, 931, 952
BsmAI GTCTC 2 cut(s) 160, 907
BsmFI GGGAC 1 cut(s) 537
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 3 cut(s) 304, 677, 803
Bso31I GGTCTC 1 cut(s) 160
Bsp119I TTCGAA 2 cut(s) 130, 411
Bsp1286I GDGCHC 2 cut(s) 761, 848
Bsp143I GATC 6 cut(s) 442, 577, 648, 819, 966, 1026
BspANI GGCC 3 cut(s) 304, 677, 803
BspCNI CTCAG 2 cut(s) 478, 1160
BspDI ATCGAT 1 cut(s) 663
BspLI GGNNCC 2 cut(s) 525, 1108
BspMI ACCTGC 1 cut(s) 555
BspT104I TTCGAA 2 cut(s) 130, 411
BspT107I GGYRCC 1 cut(s) 1106
BspTNI GGTCTC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 990
BsrFI RCCGGY 2 cut(s) 353, 1051
BsrI ACTGG 3 cut(s) 102, 190, 815
BssAI RCCGGY 2 cut(s) 353, 1051
BssECI CCNNGG 3 cut(s) 798, 930, 1095
BssMI GATC 6 cut(s) 442, 577, 648, 819, 966, 1026
BssT1I CCWWGG 2 cut(s) 930, 1095
Bst4CI ACNGT 3 cut(s) 208, 214, 607
Bst6I CTCTTC 2 cut(s) 774, 917
BstBAI YACGTR 1 cut(s) 1061
BstBI TTCGAA 2 cut(s) 130, 411
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 4 cut(s) 218, 453, 465, 1147
BstDSI CCRYGG 1 cut(s) 798
BstF5I GGATG 2 cut(s) 304, 961
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 6 cut(s) 445, 580, 651, 822, 969, 1029
BstMAI GTCTC 2 cut(s) 160, 907
BstMBI GATC 6 cut(s) 442, 577, 648, 819, 966, 1026
BstMWI GCNNNNNNNGC 4 cut(s) 243, 317, 640, 800
BstSLI GKGCMC 1 cut(s) 761
BstV1I GCAGC 4 cut(s) 392, 555, 779, 806
Bsu15I ATCGAT 1 cut(s) 663
BsuI GTATCC 2 cut(s) 1066, 1102
BsuRI GGCC 3 cut(s) 304, 677, 803
BsuTUI ATCGAT 1 cut(s) 663
BtgI CCRYGG 1 cut(s) 798
BtgZI GCGATG 1 cut(s) 885
BtsCI GGATG 2 cut(s) 304, 961
BtsIMutI CAGTG 2 cut(s) 603, 1121
BveI ACCTGC 1 cut(s) 555
Cac8I GCNNGC 1 cut(s) 378
CfoI GCGC 1 cut(s) 767
Cfr10I RCCGGY 2 cut(s) 353, 1051
Cfr13I GGNCC 2 cut(s) 302, 524
ClaI ATCGAT 1 cut(s) 663
Csp6I GTAC 3 cut(s) 215, 544, 1107
CspAI ACCGGT 1 cut(s) 353
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 5 cut(s) 373, 406, 520, 776, 899
CviQI GTAC 3 cut(s) 215, 544, 1107
DdeI CTNAG 4 cut(s) 218, 453, 465, 1147
DpnI GATC 6 cut(s) 444, 579, 650, 821, 968, 1028
DpnII GATC 6 cut(s) 442, 577, 648, 819, 966, 1026
DrdI GACNNNNNNGTC 1 cut(s) 899
DseDI GACNNNNNNGTC 1 cut(s) 899
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 2 cut(s) 774, 917
EarI CTCTTC 2 cut(s) 774, 917
Eco130I CCWWGG 2 cut(s) 930, 1095
Eco31I GGTCTC 1 cut(s) 160
Eco32I GATATC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 524
Eco57I CTGAAG 2 cut(s) 272, 623
Eco72I CACGTG 1 cut(s) 1061
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 930, 1095
ErhI CCWWGG 2 cut(s) 930, 1095
FaeI CATG 5 cut(s) 376, 409, 523, 779, 902
FaqI GGGAC 1 cut(s) 537
FatI CATG 5 cut(s) 372, 405, 519, 775, 898
FbaI TGATCA 1 cut(s) 819
Fnu4HI GCNGC 5 cut(s) 6, 381, 569, 768, 795
FokI GGATG 2 cut(s) 311, 968
Fsp4HI GCNGC 5 cut(s) 6, 381, 569, 768, 795
FspBI CTAG 1 cut(s) 743
GlaI GCGC 1 cut(s) 766
GluI GCNGC 5 cut(s) 6, 381, 569, 768, 795
GsaI CCCAGC 1 cut(s) 258
HaeIII GGCC 3 cut(s) 304, 677, 803
HapII CCGG 4 cut(s) 342, 354, 1052, 1077
HhaI GCGC 1 cut(s) 767
Hin1II CATG 5 cut(s) 376, 409, 523, 779, 902
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 3 cut(s) 77, 284, 1160
HinfI GANTC 2 cut(s) 278, 315
HpaII CCGG 4 cut(s) 342, 354, 1052, 1077
HphI GGTGA 2 cut(s) 220, 358
Hpy166II GTNNAC 2 cut(s) 892, 925
Hpy188I TCNGA 4 cut(s) 468, 718, 883, 1150
Hpy188III TCNNGA 5 cut(s) 275, 290, 487, 953, 1030
Hpy8I GTNNAC 2 cut(s) 892, 925
HpyAV CCTTC 5 cut(s) 93, 195, 647, 905, 1097
HpyCH4III ACNGT 3 cut(s) 208, 214, 607
HpyCH4IV ACGT 2 cut(s) 962, 1060
HpyCH4V TGCA 5 cut(s) 311, 380, 794, 875, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 317, 640, 800
HpyF3I CTNAG 4 cut(s) 218, 453, 465, 1147
HpySE526I ACGT 2 cut(s) 962, 1060
Hsp92II CATG 5 cut(s) 376, 409, 523, 779, 902
HspAI GCGC 1 cut(s) 765
KpnI GGTACC 1 cut(s) 1110
Ksp22I TGATCA 1 cut(s) 819
Kzo9I GATC 6 cut(s) 442, 577, 648, 819, 966, 1026
LmnI GCTCC 2 cut(s) 388, 843
Lsp1109I GCAGC 4 cut(s) 392, 555, 779, 806
LweI GCATC 4 cut(s) 298, 718, 862, 989
MaeI CTAG 1 cut(s) 743
MaeII ACGT 2 cut(s) 962, 1060
MaeIII GTNAC 2 cut(s) 208, 364
MalI GATC 6 cut(s) 444, 579, 650, 821, 968, 1028
MboI GATC 6 cut(s) 442, 577, 648, 819, 966, 1026
MboII GAAGA 6 cut(s) 643, 791, 904, 1057, 1112, 1127
MfeI CAATTG 1 cut(s) 823
MhlI GDGCHC 2 cut(s) 761, 848
MlyI GAGTC 1 cut(s) 324
MmeI TCCRAC 2 cut(s) 641, 831
MseI TTAA 5 cut(s) 15, 161, 588, 630, 1179
MslI CAYNNNNRTG 1 cut(s) 371
MspI CCGG 4 cut(s) 342, 354, 1052, 1077
MunI CAATTG 1 cut(s) 823
Mva1269I GAATGC 1 cut(s) 1039
MwoI GCNNNNNNNGC 4 cut(s) 243, 317, 640, 800
NdeII GATC 6 cut(s) 442, 577, 648, 819, 966, 1026
NlaIII CATG 5 cut(s) 376, 409, 523, 779, 902
NlaIV GGNNCC 2 cut(s) 525, 1108
NmuCI GTSAC 2 cut(s) 208, 364
NspV TTCGAA 2 cut(s) 130, 411
PctI GAATGC 1 cut(s) 1039
PfeI GAWTC 1 cut(s) 278
PflFI GACNNNGTC 1 cut(s) 152
PinAI ACCGGT 1 cut(s) 353
PkrI GCNGC 5 cut(s) 7, 382, 570, 769, 796
PleI GAGTC 1 cut(s) 323
PmaCI CACGTG 1 cut(s) 1061
PmlI CACGTG 1 cut(s) 1061
PpsI GAGTC 1 cut(s) 323
Ppu21I YACGTR 1 cut(s) 1061
PsiI TTATAA 1 cut(s) 173
PspCI CACGTG 1 cut(s) 1061
PspFI CCCAGC 1 cut(s) 254
PspN4I GGNNCC 2 cut(s) 525, 1108
PspPI GGNCC 2 cut(s) 302, 524
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 3 cut(s) 216, 545, 1108
RsaNI GTAC 3 cut(s) 215, 544, 1107
RseI CAYNNNNRTG 1 cut(s) 371
SaqAI TTAA 5 cut(s) 15, 161, 588, 630, 1179
SatI GCNGC 5 cut(s) 6, 381, 569, 768, 795
Sau3AI GATC 6 cut(s) 442, 577, 648, 819, 966, 1026
Sau96I GGNCC 2 cut(s) 302, 524
ScaI AGTACT 1 cut(s) 216
SchI GAGTC 1 cut(s) 324
SduI GDGCHC 2 cut(s) 761, 848
SfaNI GCATC 4 cut(s) 298, 718, 862, 989
SfuI TTCGAA 2 cut(s) 130, 411
SgrAI CRCCGGYG 1 cut(s) 353
SinI GGWCC 1 cut(s) 524
SmiMI CAYNNNNRTG 1 cut(s) 371
SspMI CTAG 1 cut(s) 743
StyI CCWWGG 2 cut(s) 930, 1095
TaaI ACNGT 3 cut(s) 208, 214, 607
TaiI ACGT 2 cut(s) 965, 1063
TaqI TCGA 7 cut(s) 130, 147, 166, 330, 411, 441, 663
TatI WGTACW 1 cut(s) 214
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 5 cut(s) 15, 161, 588, 630, 1179
Tru9I TTAA 5 cut(s) 15, 161, 588, 630, 1179
TscAI CASTG 2 cut(s) 610, 1128
TseFI GTSAC 2 cut(s) 208, 364
TseI GCWGC 5 cut(s) 5, 380, 568, 767, 794
Tsp45I GTSAC 2 cut(s) 208, 364
TspDTI ATGAA 4 cut(s) 44, 792, 887, 1128
TspGWI ACGGA 4 cut(s) 50, 910, 935, 1013
TspRI CASTG 2 cut(s) 610, 1128
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 3 cut(s) 136, 1006, 1066
XspI CTAG 1 cut(s) 743
ZrmI AGTACT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.