FvH4_2g34812

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001289
Physical Location & Seq
Reverse (-)
25792622 .. 25795140
2519 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34812.t1

Sequence Viewer

Length: 552 bp
ATGATGATGAGTAGCATAGCTGCTAGGAGCTCAAGTATAAATACCCCCTATTATTTCTTTCAAATGGAAACTCATTCACCGGTTAAAGAACTTGTTCTACCTATTTCCAATTCTACCTTAATTAGCATGAATCTGAGTTATATGATTGGCCGGGTACATCACATAGCAGTGTCACGTGGTGTTCCGGATAAAAAAAATAATTACTCTTTGCAAAATCCTCTTAAAGATAGCTCAAAGTTCAAAGTGATCCGAGCTATGGAAAGAACCAGTAGTTTCGCTATGCCGCCCCCAACCTTTGGAGACCTAATTACTGTTCTGAGCATCGACGGAGGTGGAATAAGAGGGATTATACCAGCCATTATCCTTGATTCCCTTGAGTCTGAACTTCAGAAGCTGGATAATAATAATACAGATGCAAGACTCGCCGACTATTTCGATGTGATTTCAGGGACAAGCACAGGTGGTCTTGTAACAGCCATGCTCACAGCTCCAAATAAGAATAACCGTCCGCTATTTGTTGCCAAAGATATCAAGGACTTCTACGAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

184

Amino Acids

20.22

Weight (kDa)

8.83

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 106 - 170 7.7e-11 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 296
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 2 cut(s) 284, 509
AclI AACGTT 1 cut(s) 547
AclWI GGATC 1 cut(s) 241
AcoI YGGCCR 1 cut(s) 148
AcuI CTGAAG 1 cut(s) 371
AcvI CACGTG 1 cut(s) 176
AdeI CACNNNGTG 1 cut(s) 179
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 256, 296
AgeI ACCGGT 1 cut(s) 79
AgsI TTSAA 2 cut(s) 62, 241
AluBI AGCT 6 cut(s) 20, 30, 231, 254, 394, 488
AluI AGCT 6 cut(s) 20, 30, 231, 254, 394, 488
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 1 cut(s) 241
AlwNI CAGNNNCTG 1 cut(s) 394
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 20
AsiGI ACCGGT 1 cut(s) 79
Asp700I GAANNNNTTC 1 cut(s) 93
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 69
BanII GRGCYC 1 cut(s) 32
BbrPI CACGTG 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 7
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 1 cut(s) 24
BisI GCNGC 2 cut(s) 21, 284
BlsI GCNGC 2 cut(s) 22, 285
Bme1390I CCNGG 1 cut(s) 152
BmrFI CCNGG 1 cut(s) 152
BmsI GCATC 2 cut(s) 330, 403
BpuEI CTTGAG 2 cut(s) 16, 395
BpuMI CCSGG 1 cut(s) 152
BsaAI YACGTR 1 cut(s) 176
BsaI GGTCTC 1 cut(s) 294
BsaWI WCCGGW 2 cut(s) 79, 184
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 296
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 267
BseAI TCCGGA 1 cut(s) 184
BseLI CCNNNNNNNGG 2 cut(s) 256, 296
BseMII CTCAG 2 cut(s) 125, 308
BseNI ACTGG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 150
BshTI ACCGGT 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 3 cut(s) 80, 151, 185
BslFI GGGAC 1 cut(s) 463
BslI CCNNNNNNNGG 2 cut(s) 256, 296
BsmAI GTCTC 1 cut(s) 294
BsmFI GGGAC 1 cut(s) 463
BsnI GGCC 1 cut(s) 150
Bso31I GGTCTC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 32
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 2 cut(s) 284, 509
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 2 cut(s) 126, 309
BspEI TCCGGA 1 cut(s) 184
BspPI GGATC 1 cut(s) 241
BspTNI GGTCTC 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 79
BssMI GATC 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 313, 506
BstBAI YACGTR 1 cut(s) 176
BstDEI CTNAG 2 cut(s) 134, 317
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 1 cut(s) 422
BstSCI CCNGG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 7
BsuRI GGCC 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 394
Cfr10I RCCGGY 1 cut(s) 79
Csp6I GTAC 1 cut(s) 155
CspAI ACCGGT 1 cut(s) 79
CviAII CATG 2 cut(s) 127, 478
CviJI RGCY 9 cut(s) 20, 30, 150, 231, 254, 356, 394, 476, 488
CviKI_1 RGCY 9 cut(s) 20, 30, 150, 231, 254, 356, 394, 476, 488
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 2 cut(s) 134, 317
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
DraIII CACNNNGTG 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 294
Eco32I GATATC 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 371
Eco72I CACGTG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 30
EcoRV GATATC 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 32
FaeI CATG 2 cut(s) 130, 481
FaqI GGGAC 1 cut(s) 463
FatI CATG 2 cut(s) 126, 477
Fnu4HI GCNGC 2 cut(s) 21, 284
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 2 cut(s) 21, 284
FspBI CTAG 1 cut(s) 24
GluI GCNGC 2 cut(s) 21, 284
HaeIII GGCC 1 cut(s) 150
HapII CCGG 3 cut(s) 80, 151, 185
Hin1II CATG 2 cut(s) 130, 481
HinfI GANTC 4 cut(s) 130, 368, 377, 420
HpaII CCGG 3 cut(s) 80, 151, 185
HphI GGTGA 1 cut(s) 69
Hpy188I TCNGA 5 cut(s) 135, 251, 318, 382, 390
Hpy188III TCNNGA 1 cut(s) 185
Hpy99I CGWCG 1 cut(s) 329
HpyCH4III ACNGT 2 cut(s) 313, 506
HpyCH4IV ACGT 2 cut(s) 175, 547
HpyCH4V TGCA 2 cut(s) 211, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 422
HpyF3I CTNAG 2 cut(s) 134, 317
HpySE526I ACGT 2 cut(s) 175, 547
Hsp92II CATG 2 cut(s) 130, 481
Kpn2I TCCGGA 1 cut(s) 184
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 2 cut(s) 27, 493
LpnPI CCDG 8 cut(s) 93, 164, 198, 280, 366, 380, 432, 444
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 2 cut(s) 330, 403
MaeI CTAG 1 cut(s) 24
MaeII ACGT 2 cut(s) 175, 547
MaeIII GTNAC 2 cut(s) 171, 469
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 4 cut(s) 109, 120, 199, 306
MlyI GAGTC 2 cut(s) 386, 414
MnlI CCTC 3 cut(s) 228, 323, 335
MroI TCCGGA 1 cut(s) 184
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 3 cut(s) 84, 119, 222
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 3 cut(s) 80, 151, 185
MspR9I CCNGG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 422
NciI CCSGG 1 cut(s) 152
NdeII GATC 1 cut(s) 246
NlaIII CATG 2 cut(s) 130, 481
NmuCI GTSAC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 2 cut(s) 130, 368
PflMI CCANNNNNTGG 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 79
PkrI GCNGC 2 cut(s) 22, 285
PleI GAGTC 2 cut(s) 385, 414
PmaCI CACGTG 1 cut(s) 176
PmlI CACGTG 1 cut(s) 176
PpsI GAGTC 2 cut(s) 385, 414
Ppu21I YACGTR 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 32
Psp1406I AACGTT 1 cut(s) 547
PspCI CACGTG 1 cut(s) 176
PsrI GAACNNNNNNTAC 2 cut(s) 81, 113
PstNI CAGNNNCTG 1 cut(s) 394
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
RseI CAYNNNNRTG 1 cut(s) 167
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 3 cut(s) 84, 119, 222
SatI GCNGC 2 cut(s) 21, 284
Sau3AI GATC 1 cut(s) 246
SchI GAGTC 2 cut(s) 386, 414
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 1 cut(s) 32
SfaNI GCATC 2 cut(s) 330, 403
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 2 cut(s) 31, 374
SmoI CTYRAG 2 cut(s) 31, 374
Sse9I AATT 4 cut(s) 109, 120, 199, 306
SsiI CCGC 2 cut(s) 284, 509
SspMI CTAG 1 cut(s) 24
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 313, 506
TaiI ACGT 2 cut(s) 178, 550
TaqI TCGA 2 cut(s) 324, 435
TasI AATT 4 cut(s) 109, 120, 199, 306
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 2 cut(s) 130, 368
Tru1I TTAA 3 cut(s) 84, 119, 222
Tru9I TTAA 3 cut(s) 84, 119, 222
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 171
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 171
TspDTI ATGAA 1 cut(s) 143
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 1 cut(s) 174
Van91I CCANNNNNTGG 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.