Rw2G013730

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001289
Physical Location & Seq
Reverse (-)
14861545 .. 14863539
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013730.1

Sequence Viewer

Length: 1164 bp
ATGTCGACTGGTTTGGCAGAGAGAAAGATGGTGACAGTGTTAAGCATTGATGGAGGTGGTATTAGAGGCATCATCCCCGGCACTATCCTCAGCTTCCTCGAATCCAAGCTTCAGGATTTGGATGGCCCGAGTGCAAGACTTGCAGATTATTTTGATATAATCGCCGGAACTAGCACAGGTGGGTTGGTCACCACCATGCTTACAGCTCCCAACAAGGACAATCGACCCATGTATGAAGCAAAGGACATCAATAACTTCTACTTGGAACAAGCACCTAAGATTTTCCCTCAGAAGAGTCGAAATAACTTCTTGACATCAATTCAAAGTTTGGTTGGTGCGGTGACCGGACCAAAATACGACGGGAAGTACCTGAGAACATTGACGACTGGGCTGCTTGGCGACCTCACTATAAAAGAAACTCTAACCAATGTCATCATTCCAACGTTCGATATCAAACACCTTCAGCCTGTGATTTTCTCAACCATTGATGCAAAAGAAAGTTCTTTGAAAAATGCAAAGCTTTCAGATATCTGCGTCAGTACCTCTGCAGCTCCAACTTTTCTCCCCGCACACTACTTTGAGGTTACGGACAAACAGGGAACAACTCGTTCTTTTGATCTCATTGATGGTGGGGTCGCTGCAAACAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGCACGAGTCAGAGCCTTTGGATGCCACTCGGTTGCTAGTGCTCTCATTGGGCACCGGGTCCGCCAAGTCTGAAGGGAAGTACACCGCAGCCGAGGCCTCAAAATGGGGTTTGATCAATTGGTTGTTTAACAATGGAAGCACACCTTTGGTTGACATGTTTGGCGATTCGAGTGCTGATATGGTTGACATTCATGTCTCCATTCTCTTCCAGTCCCTCAATTGCAACGATAACTACATACGTATTCAGGATGATAATTTGATCGGTGAGGAGGCATCAGTGGACATTGCAACAGAGAAGAATCTGAAGAGGCTTGTGGAGATCGGAAATGCGCTGTTGAAGAAGCGATTGTCAAGGGTAAATCTCGATACTGGGAGGTACGAGGAATCTGAGGGAGAAGGTACCTACGAAGAGGCACTGGTCGATTTTGCCAAACGGCTCGCGGAGGGAAGGAAGCTCAGGCAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

387

Amino Acids

42.38

Weight (kDa)

5.44

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 222 4.2e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 884
Acc65I GGTACC 1 cut(s) 1091
AccB1I GGYRCC 2 cut(s) 743, 1091
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 1133
AciI CCGC 5 cut(s) 338, 567, 753, 777, 1133
AclI AACGTT 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 660
AcuI CTGAAG 4 cut(s) 95, 446, 783, 1016
AfaI GTAC 5 cut(s) 368, 541, 773, 1070, 1093
AfiI CCNNNNNNNGG 1 cut(s) 795
AflIII ACRYGT 1 cut(s) 846
AgsI TTSAA 4 cut(s) 323, 508, 691, 1030
AluBI AGCT 6 cut(s) 93, 109, 206, 520, 551, 1147
AluI AGCT 6 cut(s) 93, 109, 206, 520, 551, 1147
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 1 cut(s) 892
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 3 cut(s) 124, 660, 786
ApeKI GCWGC 4 cut(s) 391, 548, 638, 779
Asp718I GGTACC 1 cut(s) 1091
AspLEI GCGC 1 cut(s) 1024
AspS9I GGNCC 3 cut(s) 125, 347, 750
AsuC2I CCSGG 2 cut(s) 78, 748
AsuHPI GGTGA 4 cut(s) 43, 181, 352, 968
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 2 cut(s) 347, 750
BaeGI GKGCMC 1 cut(s) 746
BalI TGGCCA 1 cut(s) 662
BanI GGYRCC 2 cut(s) 743, 1091
BauI CACGAG 1 cut(s) 695
Bbv12I GWGCWC 1 cut(s) 735
BbvCI CCTCAGC 1 cut(s) 89
BbvI GCAGC 4 cut(s) 378, 560, 625, 791
BccI CCATC 5 cut(s) 22, 44, 116, 620, 652
BceAI ACGGC 1 cut(s) 1142
BcgI CGANNNNNNTGC 4 cut(s) 373, 407, 845, 879
BclI TGATCA 1 cut(s) 804
BcnI CCSGG 2 cut(s) 78, 748
BcoDI GTCTC 1 cut(s) 892
BfaI CTAG 2 cut(s) 171, 728
BfmI CTRYAG 1 cut(s) 546
BisI GCNGC 4 cut(s) 392, 549, 639, 780
BlsI GCNGC 4 cut(s) 393, 550, 640, 781
Bme1390I CCNGG 2 cut(s) 78, 748
Bme18I GGWCC 2 cut(s) 347, 750
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 3 cut(s) 125, 347, 750
BmiI GGNNCC 3 cut(s) 745, 751, 1093
BmrFI CCNGG 2 cut(s) 78, 748
BmrI ACTGGG 2 cut(s) 396, 1071
BmsI GCATC 4 cut(s) 78, 478, 703, 974
BmuI ACTGGG 2 cut(s) 396, 1071
Bpu10I CCTNAGC 2 cut(s) 89, 1148
BpuMI CCSGG 2 cut(s) 78, 748
BsaAI YACGTR 1 cut(s) 932
BsaJI CCNNGG 2 cut(s) 76, 783
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 795
Bse1I ACTGG 5 cut(s) 13, 391, 901, 1066, 1113
Bse3DI GCAATG 1 cut(s) 975
BseDI CCNNGG 2 cut(s) 76, 783
BseGI GGATG 4 cut(s) 72, 127, 718, 946
BseLI CCNNNNNNNGG 1 cut(s) 795
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 5 cut(s) 103, 302, 362, 1071, 1162
BseNI ACTGG 5 cut(s) 13, 391, 901, 1066, 1113
BseRI GAGGAG 1 cut(s) 974
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 4 cut(s) 378, 560, 625, 791
Bsh1236I CGCG 1 cut(s) 1133
BshFI GGCC 3 cut(s) 126, 662, 788
BshNI GGYRCC 2 cut(s) 743, 1091
BsiHKAI GWGCWC 1 cut(s) 735
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 4 cut(s) 78, 165, 345, 747
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 1 cut(s) 795
BsmAI GTCTC 1 cut(s) 892
BsmFI GGGAC 1 cut(s) 889
BsnI GGCC 3 cut(s) 126, 662, 788
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 735, 746
Bsp143I GATC 4 cut(s) 616, 804, 951, 1011
BspACI CCGC 5 cut(s) 338, 567, 753, 777, 1133
BspANI GGCC 3 cut(s) 126, 662, 788
BspCNI CTCAG 5 cut(s) 102, 301, 363, 1072, 1161
BspFNI CGCG 1 cut(s) 1133
BspLI GGNNCC 3 cut(s) 745, 751, 1093
BspMAI CTGCAG 1 cut(s) 550
BspT107I GGYRCC 2 cut(s) 743, 1091
BsrDI GCAATG 1 cut(s) 975
BsrI ACTGG 5 cut(s) 13, 391, 901, 1066, 1113
BssECI CCNNGG 2 cut(s) 76, 783
BssMI GATC 4 cut(s) 616, 804, 951, 1011
BssSI CACGAG 1 cut(s) 695
Bst2BI CACGAG 1 cut(s) 695
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 4 cut(s) 287, 902, 992, 1095
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 932
BstC8I GCNNGC 1 cut(s) 1131
BstDEI CTNAG 6 cut(s) 89, 276, 288, 371, 1080, 1148
BstEII GGTNACC 2 cut(s) 187, 340
BstF5I GGATG 4 cut(s) 72, 127, 718, 946
BstFNI CGCG 1 cut(s) 1133
BstHHI GCGC 1 cut(s) 1024
BstKTI GATC 4 cut(s) 619, 807, 954, 1014
BstMAI GTCTC 1 cut(s) 892
BstMBI GATC 4 cut(s) 616, 804, 951, 1011
BstMWI GCNNNNNNNGC 2 cut(s) 140, 785
BstNSI RCATGY 1 cut(s) 850
BstPI GGTNACC 2 cut(s) 187, 340
BstSCI CCNGG 2 cut(s) 76, 746
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 746
BstSNI TACGTA 1 cut(s) 932
BstUI CGCG 1 cut(s) 1133
BstV1I GCAGC 4 cut(s) 378, 560, 625, 791
BsuRI GGCC 3 cut(s) 126, 662, 788
BtsCI GGATG 4 cut(s) 72, 127, 718, 946
BtsIMutI CAGTG 3 cut(s) 42, 975, 1106
Cac8I GCNNGC 1 cut(s) 1131
CfoI GCGC 1 cut(s) 1024
Cfr13I GGNCC 3 cut(s) 125, 347, 750
CseI GACGC 1 cut(s) 523
Csp6I GTAC 5 cut(s) 367, 540, 772, 1069, 1092
CviAII CATG 4 cut(s) 196, 229, 847, 884
CviQI GTAC 5 cut(s) 367, 540, 772, 1069, 1092
DdeI CTNAG 6 cut(s) 89, 276, 288, 371, 1080, 1148
DpnI GATC 4 cut(s) 618, 806, 953, 1013
DpnII GATC 4 cut(s) 616, 804, 951, 1011
DrdI GACNNNNNNGTC 1 cut(s) 884
DseDI GACNNNNNNGTC 1 cut(s) 884
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 4 cut(s) 287, 902, 992, 1095
EarI CTCTTC 4 cut(s) 287, 902, 992, 1095
EciI GGCGGA 1 cut(s) 742
Eco105I TACGTA 1 cut(s) 932
Eco147I AGGCCT 1 cut(s) 788
Eco32I GATATC 2 cut(s) 451, 529
Eco47I GGWCC 2 cut(s) 347, 750
Eco57I CTGAAG 4 cut(s) 95, 446, 783, 1016
Eco88I CYCGRG 1 cut(s) 127
Eco91I GGTNACC 2 cut(s) 187, 340
EcoO65I GGTNACC 2 cut(s) 187, 340
EcoRV GATATC 2 cut(s) 451, 529
FaeI CATG 4 cut(s) 199, 232, 850, 887
FalI AAGNNNNNCTT 2 cut(s) 820, 852
FaqI GGGAC 1 cut(s) 889
FatI CATG 4 cut(s) 195, 228, 846, 883
FauI CCCGC 1 cut(s) 574
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 392, 549, 639, 780
FokI GGATG 4 cut(s) 59, 134, 725, 953
Fsp4HI GCNGC 4 cut(s) 392, 549, 639, 780
FspBI CTAG 2 cut(s) 171, 728
GlaI GCGC 1 cut(s) 1023
GluI GCNGC 4 cut(s) 392, 549, 639, 780
HaeIII GGCC 3 cut(s) 126, 662, 788
HapII CCGG 4 cut(s) 78, 165, 345, 747
HgaI GACGC 1 cut(s) 523
HhaI GCGC 1 cut(s) 1024
Hin1II CATG 4 cut(s) 199, 232, 850, 887
Hin6I GCGC 1 cut(s) 1022
HinP1I GCGC 1 cut(s) 1022
HincII GTYRAC 3 cut(s) 6, 844, 877
HindII GTYRAC 3 cut(s) 6, 844, 877
HindIII AAGCTT 2 cut(s) 107, 518
HinfI GANTC 6 cut(s) 101, 295, 698, 857, 991, 1076
HpaII CCGG 4 cut(s) 78, 165, 345, 747
HphI GGTGA 4 cut(s) 43, 181, 352, 968
Hpy166II GTNNAC 5 cut(s) 6, 774, 844, 877, 973
Hpy188I TCNGA 7 cut(s) 291, 526, 703, 763, 996, 1016, 1081
Hpy188III TCNNGA 4 cut(s) 113, 310, 938, 1055
Hpy8I GTNNAC 5 cut(s) 6, 774, 844, 877, 973
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 4 cut(s) 470, 758, 1082, 1134
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 2 cut(s) 443, 931
HpyCH4V TGCA 8 cut(s) 134, 143, 491, 515, 548, 641, 915, 980
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 785
HpyF3I CTNAG 6 cut(s) 89, 276, 288, 371, 1080, 1148
HpySE526I ACGT 2 cut(s) 443, 931
Hsp92II CATG 4 cut(s) 199, 232, 850, 887
HspAI GCGC 1 cut(s) 1022
KpnI GGTACC 1 cut(s) 1095
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 4 cut(s) 616, 804, 951, 1011
LmnI GCTCC 2 cut(s) 211, 556
Lsp1109I GCAGC 4 cut(s) 378, 560, 625, 791
LweI GCATC 4 cut(s) 78, 478, 703, 974
MaeI CTAG 2 cut(s) 171, 728
MaeII ACGT 2 cut(s) 443, 931
MaeIII GTNAC 4 cut(s) 31, 187, 340, 583
MalI GATC 4 cut(s) 618, 806, 953, 1013
MboI GATC 4 cut(s) 616, 804, 951, 1011
MboII GAAGA 6 cut(s) 304, 889, 1000, 1009, 1042, 1112
MfeI CAATTG 2 cut(s) 808, 910
MhlI GDGCHC 2 cut(s) 735, 746
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 5 cut(s) 318, 808, 910, 946, 1159
MluNI TGGCCA 1 cut(s) 662
MlyI GAGTC 2 cut(s) 304, 707
MmeI TCCRAC 2 cut(s) 464, 578
Mox20I TGGCCA 1 cut(s) 662
MscI TGGCCA 1 cut(s) 662
MseI TTAA 2 cut(s) 41, 819
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 662
MspI CCGG 4 cut(s) 78, 165, 345, 747
MspR9I CCNGG 2 cut(s) 78, 748
MunI CAATTG 2 cut(s) 808, 910
MvnI CGCG 1 cut(s) 1133
MwoI GCNNNNNNNGC 2 cut(s) 140, 785
NciI CCSGG 2 cut(s) 78, 748
NdeII GATC 4 cut(s) 616, 804, 951, 1011
NlaIII CATG 4 cut(s) 199, 232, 850, 887
NlaIV GGNNCC 3 cut(s) 745, 751, 1093
NmeAIII GCCGAG 1 cut(s) 808
NmuCI GTSAC 3 cut(s) 31, 187, 340
NspI RCATGY 1 cut(s) 850
PceI AGGCCT 1 cut(s) 788
PciI ACATGT 1 cut(s) 846
PfeI GAWTC 4 cut(s) 101, 857, 991, 1076
PkrI GCNGC 4 cut(s) 393, 550, 640, 781
PleI GAGTC 2 cut(s) 303, 706
PpsI GAGTC 2 cut(s) 303, 706
Ppu21I YACGTR 1 cut(s) 932
PscI ACATGT 1 cut(s) 846
Psp1406I AACGTT 1 cut(s) 443
PspEI GGTNACC 2 cut(s) 187, 340
PspN4I GGNNCC 3 cut(s) 745, 751, 1093
PspPI GGNCC 3 cut(s) 125, 347, 750
PstI CTGCAG 1 cut(s) 550
RsaI GTAC 5 cut(s) 368, 541, 773, 1070, 1093
RsaNI GTAC 5 cut(s) 367, 540, 772, 1069, 1092
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 41, 819
SatI GCNGC 4 cut(s) 392, 549, 639, 780
Sau3AI GATC 4 cut(s) 616, 804, 951, 1011
Sau96I GGNCC 3 cut(s) 125, 347, 750
SchI GAGTC 2 cut(s) 304, 707
ScrFI CCNGG 2 cut(s) 78, 748
SduI GDGCHC 2 cut(s) 735, 746
SfaNI GCATC 4 cut(s) 78, 478, 703, 974
SfcI CTRYAG 1 cut(s) 546
SinI GGWCC 2 cut(s) 347, 750
SmiMI CAYNNNNRTG 1 cut(s) 194
SnaBI TACGTA 1 cut(s) 932
Sse9I AATT 5 cut(s) 318, 808, 910, 946, 1159
SseBI AGGCCT 1 cut(s) 788
SsiI CCGC 5 cut(s) 338, 567, 753, 777, 1133
SspMI CTAG 2 cut(s) 171, 728
StuI AGGCCT 1 cut(s) 788
StyD4I CCNGG 2 cut(s) 76, 746
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 2 cut(s) 446, 934
TaqI TCGA 8 cut(s) 5, 99, 223, 298, 447, 860, 1056, 1113
TasI AATT 5 cut(s) 318, 808, 910, 946, 1159
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 4 cut(s) 101, 857, 991, 1076
Tru1I TTAA 2 cut(s) 41, 819
Tru9I TTAA 2 cut(s) 41, 819
TscAI CASTG 3 cut(s) 42, 975, 1113
TseFI GTSAC 3 cut(s) 31, 187, 340
TseI GCWGC 4 cut(s) 391, 548, 638, 779
Tsp45I GTSAC 3 cut(s) 31, 187, 340
TspDTI ATGAA 2 cut(s) 249, 872
TspGWI ACGGA 1 cut(s) 602
TspRI CASTG 3 cut(s) 42, 975, 1113
VpaK11BI GGWCC 2 cut(s) 347, 750
XceI RCATGY 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 171, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.