Rh5BG275200

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001289
Physical Location & Seq
Reverse (-)
34657583 .. 34660388
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG275200.1

Sequence Viewer

Length: 1137 bp
ATGTCTACTGGTTTGGCAAAGAGAAAGATGGTGACAGTGTTAAGCATTGACGGAGGTGGTATTAGAGGCATCATCCCCGGCACTATCCTCGGCTTTCTCGAATCCAAGCTTCAGGATTTGGATGGCCCGAGTGCAAGACTTGCCGATTATTTTGATATAATCGCCGGAACTAGCACAGCTCCCAACAAGGACAATCGACCCATGTATGAAGCAAAGGACATCAATAACTTCTACTTGGAACAAACACCTAAGATTTTCCCTCAGAAGAGTCGAAAGAACTTCTTGACATCAATTCAAAGTTTGGTTGGAGCGGTGACCGGACCAAAATACGACGGGAATTACCTGCGCACATTGACGACCGGGCTGCTTGGCGACCTCACAATAAAAGAAACTCTAACCAATGTCATCATCCCAACGTTCGATATCAAACACCTTCAGCCTGTGATTTTCTCAACCATTGATGCAAAAGAAAGTTCTTTGAAAAATGCAAAGCTTTCAGATATCTGCATCAGTACCTCTGCAGCTCCAACTTTTCTCCCCGCACACTACTTTGAGGTTACGGACAAACAGGGAACAACTCGTTCTTTTGATCTCGTTGATGGTGGGGTCGCTGCAAATAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGCACGACTCAGAGCCATTGGATGCCACTCGGTTACTAGTGCTCTCATTGGGCACCGGCTCCGCCAAGGCTGAAGGGAAGTACACCTCAGCCGAGGCCTCAAAATGGGGTTTGATCAATTGGTTGTTTAACAATGGGAGCACACCTTTGGTTGACATGTTTGGCGATGCGAGTGCTGATATGGTTGACATTCATGTCTCCACTCTGTTCCAGTCCCTCAATTGCAACGATAACTACATACGCATACAGGATGATAATTTGATCGGTGAGGAGGCATCAGTGGACATTGCAACAGAGAAGAATCTGAAGAGGCTTGTGGAGATCGGAAATGCGCTGTTGAAGAAGCGATTGTCAAGGGTAAATCTCGATACTGGGAGGTACGAGGAATCTGAGGGAGAAGGTACCTACGAAGAAGCACTGGTCGATTTTGCCAAACGGCTCGCGGAGGGAAGGAAGCTCAGGCAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

378

Amino Acids

41.46

Weight (kDa)

5.69

Isoelectric Point (pI)

32.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 104 - 213 1.8e-11 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
Acc16I TGCGCA 1 cut(s) 347
Acc36I ACCTGC 1 cut(s) 351
Acc65I GGTACC 1 cut(s) 1064
AccB1I GGYRCC 2 cut(s) 716, 1064
AccBSI CCGCTC 1 cut(s) 311
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 1106
AciI CCGC 4 cut(s) 311, 540, 726, 1106
AclI AACGTT 1 cut(s) 416
AcoI YGGCCR 1 cut(s) 633
AcuI CTGAAG 4 cut(s) 95, 419, 756, 989
AfaI GTAC 4 cut(s) 514, 746, 1043, 1066
AfiI CCNNNNNNNGG 1 cut(s) 768
AflIII ACRYGT 1 cut(s) 819
AgsI TTSAA 4 cut(s) 296, 481, 664, 1003
AhlI ACTAGT 1 cut(s) 700
AluBI AGCT 5 cut(s) 109, 179, 493, 524, 1120
AluI AGCT 5 cut(s) 109, 179, 493, 524, 1120
Alw21I GWGCWC 2 cut(s) 708, 806
Alw26I GTCTC 1 cut(s) 865
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 3 cut(s) 124, 633, 759
ApeKI GCWGC 3 cut(s) 364, 521, 611
Asp718I GGTACC 1 cut(s) 1064
AspLEI GCGC 2 cut(s) 348, 997
AspS9I GGNCC 2 cut(s) 125, 320
AsuC2I CCSGG 2 cut(s) 78, 361
AsuHPI GGTGA 3 cut(s) 43, 325, 941
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 320
BaeGI GKGCMC 1 cut(s) 719
BalI TGGCCA 1 cut(s) 635
BanI GGYRCC 2 cut(s) 716, 1064
Bbv12I GWGCWC 2 cut(s) 708, 806
BbvCI CCTCAGC 1 cut(s) 751
BbvI GCAGC 3 cut(s) 351, 533, 598
BccI CCATC 4 cut(s) 22, 116, 593, 625
BceAI ACGGC 1 cut(s) 1115
BcgI CGANNNNNNTGC 6 cut(s) 70, 104, 346, 380, 818, 852
BclI TGATCA 1 cut(s) 777
BcnI CCSGG 2 cut(s) 78, 361
BcoDI GTCTC 1 cut(s) 865
BcuI ACTAGT 1 cut(s) 700
BfaI CTAG 2 cut(s) 171, 701
BfmI CTRYAG 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 351
BisI GCNGC 3 cut(s) 365, 522, 612
BlsI GCNGC 3 cut(s) 366, 523, 613
Bme1390I CCNGG 2 cut(s) 78, 361
Bme18I GGWCC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 125, 320
BmiI GGNNCC 3 cut(s) 718, 724, 1066
BmrFI CCNGG 2 cut(s) 78, 361
BmrI ACTGGG 1 cut(s) 1044
BmsI GCATC 6 cut(s) 78, 451, 516, 676, 820, 947
BmuI ACTGGG 1 cut(s) 1044
Bpu10I CCTNAGC 2 cut(s) 751, 1121
BpuMI CCSGG 2 cut(s) 78, 361
BsaJI CCNNGG 4 cut(s) 76, 88, 729, 756
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 1 cut(s) 768
Bse118I RCCGGY 1 cut(s) 719
Bse1I ACTGG 4 cut(s) 13, 874, 1039, 1086
Bse3DI GCAATG 1 cut(s) 948
BseDI CCNNGG 4 cut(s) 76, 88, 729, 756
BseGI GGATG 5 cut(s) 72, 127, 408, 691, 919
BseLI CCNNNNNNNGG 1 cut(s) 768
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 5 cut(s) 275, 687, 765, 1044, 1135
BseNI ACTGG 4 cut(s) 13, 874, 1039, 1086
BseRI GAGGAG 1 cut(s) 947
BseSI GKGCMC 1 cut(s) 719
BseXI GCAGC 3 cut(s) 351, 533, 598
Bsh1236I CGCG 1 cut(s) 1106
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 3 cut(s) 126, 635, 761
BshNI GGYRCC 2 cut(s) 716, 1064
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 708, 806
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 5 cut(s) 78, 165, 318, 360, 720
BslFI GGGAC 1 cut(s) 862
BslI CCNNNNNNNGG 1 cut(s) 768
BsmAI GTCTC 1 cut(s) 865
BsmFI GGGAC 1 cut(s) 862
BsnI GGCC 3 cut(s) 126, 635, 761
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 3 cut(s) 708, 719, 806
Bsp143I GATC 4 cut(s) 589, 777, 924, 984
BspACI CCGC 4 cut(s) 311, 540, 726, 1106
BspANI GGCC 3 cut(s) 126, 635, 761
BspCNI CTCAG 5 cut(s) 274, 686, 764, 1045, 1134
BspFNI CGCG 1 cut(s) 1106
BspLI GGNNCC 3 cut(s) 718, 724, 1066
BspMAI CTGCAG 1 cut(s) 523
BspMI ACCTGC 1 cut(s) 351
BspT107I GGYRCC 2 cut(s) 716, 1064
BsrBI CCGCTC 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 948
BsrFI RCCGGY 1 cut(s) 719
BsrI ACTGG 4 cut(s) 13, 874, 1039, 1086
BssAI RCCGGY 1 cut(s) 719
BssECI CCNNGG 4 cut(s) 76, 88, 729, 756
BssMI GATC 4 cut(s) 589, 777, 924, 984
BssT1I CCWWGG 1 cut(s) 729
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 2 cut(s) 260, 965
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 1104
BstDEI CTNAG 6 cut(s) 249, 261, 673, 751, 1053, 1121
BstEII GGTNACC 1 cut(s) 313
BstF5I GGATG 5 cut(s) 72, 127, 408, 691, 919
BstFNI CGCG 1 cut(s) 1106
BstHHI GCGC 2 cut(s) 348, 997
BstKTI GATC 4 cut(s) 592, 780, 927, 987
BstMAI GTCTC 1 cut(s) 865
BstMBI GATC 4 cut(s) 589, 777, 924, 984
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 823
BstPI GGTNACC 1 cut(s) 313
BstSCI CCNGG 2 cut(s) 76, 359
BstSFI CTRYAG 1 cut(s) 519
BstSLI GKGCMC 1 cut(s) 719
BstUI CGCG 1 cut(s) 1106
BstV1I GCAGC 3 cut(s) 351, 533, 598
BsuRI GGCC 3 cut(s) 126, 635, 761
BtgZI GCGATG 1 cut(s) 843
BtsCI GGATG 5 cut(s) 72, 127, 408, 691, 919
BtsIMutI CAGTG 3 cut(s) 42, 948, 1079
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 1 cut(s) 1104
CfoI GCGC 2 cut(s) 348, 997
Cfr10I RCCGGY 1 cut(s) 719
Cfr13I GGNCC 2 cut(s) 125, 320
Csp6I GTAC 4 cut(s) 513, 745, 1042, 1065
CviAII CATG 3 cut(s) 202, 820, 857
CviQI GTAC 4 cut(s) 513, 745, 1042, 1065
DdeI CTNAG 6 cut(s) 249, 261, 673, 751, 1053, 1121
DpnI GATC 4 cut(s) 591, 779, 926, 986
DpnII GATC 4 cut(s) 589, 777, 924, 984
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 2 cut(s) 260, 965
EarI CTCTTC 2 cut(s) 260, 965
EciI GGCGGA 1 cut(s) 715
Eco130I CCWWGG 1 cut(s) 729
Eco147I AGGCCT 1 cut(s) 761
Eco32I GATATC 2 cut(s) 424, 502
Eco47I GGWCC 1 cut(s) 320
Eco57I CTGAAG 4 cut(s) 95, 419, 756, 989
Eco88I CYCGRG 1 cut(s) 127
Eco91I GGTNACC 1 cut(s) 313
EcoO65I GGTNACC 1 cut(s) 313
EcoRV GATATC 2 cut(s) 424, 502
EcoT14I CCWWGG 1 cut(s) 729
ErhI CCWWGG 1 cut(s) 729
FaeI CATG 3 cut(s) 205, 823, 860
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 1 cut(s) 862
FatI CATG 3 cut(s) 201, 819, 856
FauI CCCGC 1 cut(s) 547
FbaI TGATCA 1 cut(s) 777
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 365, 522, 612
FokI GGATG 5 cut(s) 59, 134, 395, 698, 926
Fsp4HI GCNGC 3 cut(s) 365, 522, 612
FspBI CTAG 2 cut(s) 171, 701
FspI TGCGCA 1 cut(s) 347
GlaI GCGC 2 cut(s) 347, 996
GluI GCNGC 3 cut(s) 365, 522, 612
HaeIII GGCC 3 cut(s) 126, 635, 761
HapII CCGG 5 cut(s) 78, 165, 318, 360, 720
HhaI GCGC 2 cut(s) 348, 997
Hin1II CATG 3 cut(s) 205, 823, 860
Hin6I GCGC 2 cut(s) 346, 995
HinP1I GCGC 2 cut(s) 346, 995
HincII GTYRAC 2 cut(s) 817, 850
HindII GTYRAC 2 cut(s) 817, 850
HindIII AAGCTT 2 cut(s) 107, 491
HinfI GANTC 5 cut(s) 101, 268, 671, 964, 1049
HpaII CCGG 5 cut(s) 78, 165, 318, 360, 720
HphI GGTGA 3 cut(s) 43, 325, 941
Hpy166II GTNNAC 5 cut(s) 6, 747, 817, 850, 946
Hpy188I TCNGA 6 cut(s) 264, 499, 676, 969, 989, 1054
Hpy188III TCNNGA 4 cut(s) 98, 113, 283, 1028
Hpy8I GTNNAC 5 cut(s) 6, 747, 817, 850, 946
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 4 cut(s) 443, 731, 1055, 1107
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 8 cut(s) 134, 464, 488, 507, 521, 614, 888, 953
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 6 cut(s) 249, 261, 673, 751, 1053, 1121
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 3 cut(s) 205, 823, 860
HspAI GCGC 2 cut(s) 346, 995
KpnI GGTACC 1 cut(s) 1068
Ksp22I TGATCA 1 cut(s) 777
Kzo9I GATC 4 cut(s) 589, 777, 924, 984
LmnI GCTCC 5 cut(s) 184, 308, 529, 728, 801
Lsp1109I GCAGC 3 cut(s) 351, 533, 598
LweI GCATC 6 cut(s) 78, 451, 516, 676, 820, 947
MaeI CTAG 2 cut(s) 171, 701
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 4 cut(s) 31, 313, 556, 696
MalI GATC 4 cut(s) 591, 779, 926, 986
MbiI CCGCTC 1 cut(s) 311
MboI GATC 4 cut(s) 589, 777, 924, 984
MboII GAAGA 5 cut(s) 277, 973, 982, 1015, 1085
MfeI CAATTG 2 cut(s) 781, 883
MhlI GDGCHC 3 cut(s) 708, 719, 806
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 6 cut(s) 291, 337, 781, 883, 919, 1132
MluNI TGGCCA 1 cut(s) 635
MlyI GAGTC 2 cut(s) 277, 665
MmeI TCCRAC 2 cut(s) 286, 551
Mox20I TGGCCA 1 cut(s) 635
MscI TGGCCA 1 cut(s) 635
MseI TTAA 2 cut(s) 41, 792
Msp20I TGGCCA 1 cut(s) 635
MspI CCGG 5 cut(s) 78, 165, 318, 360, 720
MspR9I CCNGG 2 cut(s) 78, 361
MunI CAATTG 2 cut(s) 781, 883
MvnI CGCG 1 cut(s) 1106
MwoI GCNNNNNNNGC 1 cut(s) 140
NciI CCSGG 2 cut(s) 78, 361
NdeII GATC 4 cut(s) 589, 777, 924, 984
NlaIII CATG 3 cut(s) 205, 823, 860
NlaIV GGNNCC 3 cut(s) 718, 724, 1066
NmeAIII GCCGAG 2 cut(s) 69, 781
NmuCI GTSAC 2 cut(s) 31, 313
NsbI TGCGCA 1 cut(s) 347
NspI RCATGY 1 cut(s) 823
PceI AGGCCT 1 cut(s) 761
PciI ACATGT 1 cut(s) 819
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 3 cut(s) 101, 964, 1049
PkrI GCNGC 3 cut(s) 366, 523, 613
PleI GAGTC 2 cut(s) 276, 665
PpsI GAGTC 2 cut(s) 276, 665
PscI ACATGT 1 cut(s) 819
Psp1406I AACGTT 1 cut(s) 416
PspEI GGTNACC 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 718, 724, 1066
PspPI GGNCC 2 cut(s) 125, 320
PstI CTGCAG 1 cut(s) 523
RsaI GTAC 4 cut(s) 514, 746, 1043, 1066
RsaNI GTAC 4 cut(s) 513, 745, 1042, 1065
SaqAI TTAA 2 cut(s) 41, 792
SatI GCNGC 3 cut(s) 365, 522, 612
Sau3AI GATC 4 cut(s) 589, 777, 924, 984
Sau96I GGNCC 2 cut(s) 125, 320
SchI GAGTC 2 cut(s) 277, 665
ScrFI CCNGG 2 cut(s) 78, 361
SduI GDGCHC 3 cut(s) 708, 719, 806
SfaNI GCATC 6 cut(s) 78, 451, 516, 676, 820, 947
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 1 cut(s) 320
SpeI ACTAGT 1 cut(s) 700
Sse9I AATT 6 cut(s) 291, 337, 781, 883, 919, 1132
SseBI AGGCCT 1 cut(s) 761
SsiI CCGC 4 cut(s) 311, 540, 726, 1106
SspMI CTAG 2 cut(s) 171, 701
StuI AGGCCT 1 cut(s) 761
StyD4I CCNGG 2 cut(s) 76, 359
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 1 cut(s) 419
TaqI TCGA 6 cut(s) 99, 196, 271, 420, 1029, 1086
TasI AATT 6 cut(s) 291, 337, 781, 883, 919, 1132
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 3 cut(s) 101, 964, 1049
Tru1I TTAA 2 cut(s) 41, 792
Tru9I TTAA 2 cut(s) 41, 792
TscAI CASTG 3 cut(s) 42, 948, 1086
TseFI GTSAC 2 cut(s) 31, 313
TseI GCWGC 3 cut(s) 364, 521, 611
Tsp45I GTSAC 2 cut(s) 31, 313
TspDTI ATGAA 2 cut(s) 222, 845
TspGWI ACGGA 2 cut(s) 66, 575
TspRI CASTG 3 cut(s) 42, 948, 1086
VpaK11BI GGWCC 1 cut(s) 320
XceI RCATGY 1 cut(s) 823
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 171, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.