FvH4_1g23441

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001157
Physical Location & Seq
Forward (+)
15305390 .. 15305815
426 bp
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UTR
Exon/CDS
Intron
FvH4_1g23441.t1

Sequence Viewer

Length: 426 bp
ATGGCCTTCACAAAATTGTCCTCTAGAGCATATGGTTGGACCGAGGTACAACCCTTACTAATTTTTCTAGGTGGATTAAAACTTGAAATTAGACATGATGTTCTTGTCATGGAGCCCCACACTTTGGCGACAGCCCAACGCCTAGCTCGATTGTATGAGGCGAAGCTTGTTGACATTAAGGCTTCTCAGTTCGCCTCTTCCATAACTCGTTCATTGCACCACACCCACGAACAAGAGGTACTCACCAAAACTCCTTTCAGACAGTTGTCTTCTCGTGATCATCAGGAAAGAAGAGCTCAAGGTCTTTGCTTTAGTTATGATGAGAAATGGTCGAGGTCACATGTTTGTAAAAAGCCGATTATGGCTATTTTGGCGTCTCTGGTTCATGGTGAAGAGGTCGTTGTAGATGACATTTCAAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

142

Amino Acids

16.08

Weight (kDa)

7.88

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 124
AcyI GRCGYC 1 cut(s) 374
AfaI GTAC 2 cut(s) 48, 240
AfiI CCNNNNNNNGG 1 cut(s) 124
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 2 cut(s) 86, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AluBI AGCT 3 cut(s) 146, 166, 296
AluI AGCT 3 cut(s) 146, 166, 296
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 1 cut(s) 381
AoxI GGCC 1 cut(s) 3
AspS9I GGNCC 1 cut(s) 39
AsuHPI GGTGA 2 cut(s) 235, 401
AvaII GGWCC 1 cut(s) 39
BanII GRGCYC 2 cut(s) 117, 298
BauI CACGAG 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 298
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 3 cut(s) 24, 68, 143
Bme18I GGWCC 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 1 cut(s) 114
BoxI GACNNNNGTC 1 cut(s) 265
BpiI GAAGAC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 42
BsaXI ACNNNNNCTCC 2 cut(s) 235, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 42
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 1 cut(s) 212
BseMII CTCAG 1 cut(s) 200
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 381
BsmBI CGTCTC 1 cut(s) 381
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 117, 298
Bsp143I GATC 1 cut(s) 277
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 114
BspQI GCTCTTC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 1 cut(s) 277
BssNI GRCGYC 1 cut(s) 374
BssSI CACGAG 1 cut(s) 273
Bst2BI CACGAG 1 cut(s) 273
Bst4CI ACNGT 1 cut(s) 264
Bst6I CTCTTC 3 cut(s) 202, 286, 387
BstACI GRCGYC 1 cut(s) 374
BstDEI CTNAG 1 cut(s) 186
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 1 cut(s) 277
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstNSI RCATGY 1 cut(s) 344
BstPAI GACNNNNGTC 1 cut(s) 265
BstV2I GAAGAC 1 cut(s) 261
BsuRI GGCC 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 39
CseI GACGC 1 cut(s) 363
Csp6I GTAC 2 cut(s) 47, 239
CviAII CATG 4 cut(s) 95, 109, 341, 386
CviJI RGCY 9 cut(s) 5, 115, 134, 146, 166, 182, 296, 355, 365
CviKI_1 RGCY 9 cut(s) 5, 115, 134, 146, 166, 182, 296, 355, 365
CviQI GTAC 2 cut(s) 47, 239
DdeI CTNAG 1 cut(s) 186
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
Eam1104I CTCTTC 3 cut(s) 202, 286, 387
EarI CTCTTC 3 cut(s) 202, 286, 387
Ecl136II GAGCTC 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 117, 298
Eco47I GGWCC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 117, 298
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 4 cut(s) 98, 112, 344, 389
FatI CATG 4 cut(s) 94, 108, 340, 385
FauNDI CATATG 1 cut(s) 31
FbaI TGATCA 1 cut(s) 277
FriOI GRGCYC 2 cut(s) 117, 298
FspBI CTAG 3 cut(s) 24, 68, 143
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 363
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 4 cut(s) 98, 112, 344, 389
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 164
HphI GGTGA 2 cut(s) 235, 401
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 260
Hpy188III TCNNGA 3 cut(s) 24, 275, 284
Hpy8I GTNNAC 1 cut(s) 172
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 1 cut(s) 264
HpyCH4V TGCA 1 cut(s) 217
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 4 cut(s) 98, 112, 344, 389
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 1 cut(s) 277
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 112
LpnPI CCDG 2 cut(s) 269, 365
MaeI CTAG 3 cut(s) 24, 68, 143
MaeIII GTNAC 2 cut(s) 336, 419
MalI GATC 1 cut(s) 279
MboI GATC 1 cut(s) 277
MboII GAAGA 4 cut(s) 189, 261, 303, 404
MhlI GDGCHC 2 cut(s) 117, 298
MluCI AATT 3 cut(s) 14, 60, 87
MmeI TCCRAC 1 cut(s) 17
MnlI CCTC 7 cut(s) 31, 37, 151, 205, 229, 327, 388
MseI TTAA 2 cut(s) 77, 177
MwoI GCNNNNNNNGC 1 cut(s) 371
NdeI CATATG 1 cut(s) 31
NdeII GATC 1 cut(s) 277
NlaIII CATG 4 cut(s) 98, 112, 344, 389
NlaIV GGNNCC 1 cut(s) 114
NmuCI GTSAC 2 cut(s) 336, 419
NspI RCATGY 1 cut(s) 344
PciI ACATGT 1 cut(s) 340
PciSI GCTCTTC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 145
PflMI CCANNNNNTGG 1 cut(s) 124
PscI ACATGT 1 cut(s) 340
PshAI GACNNNNGTC 1 cut(s) 265
Psp124BI GAGCTC 1 cut(s) 298
PspN4I GGNNCC 1 cut(s) 114
PspPI GGNCC 1 cut(s) 39
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
RsaI GTAC 2 cut(s) 48, 240
RsaNI GTAC 2 cut(s) 47, 239
SacI GAGCTC 1 cut(s) 298
SapI GCTCTTC 1 cut(s) 286
SaqAI TTAA 2 cut(s) 77, 177
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 1 cut(s) 39
SduI GDGCHC 2 cut(s) 117, 298
SetI ASST 9 cut(s) 48, 73, 148, 168, 240, 298, 304, 338, 399
SinI GGWCC 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 3 cut(s) 14, 60, 87
SspMI CTAG 3 cut(s) 24, 68, 143
SstI GAGCTC 1 cut(s) 298
TaaI ACNGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 148, 332
TaqII GACCGA 1 cut(s) 56
TasI AATT 3 cut(s) 14, 60, 87
Tru1I TTAA 2 cut(s) 77, 177
Tru9I TTAA 2 cut(s) 77, 177
TseFI GTSAC 2 cut(s) 336, 419
Tsp45I GTSAC 2 cut(s) 336, 419
TspDTI ATGAA 2 cut(s) 201, 374
Van91I CCANNNNNTGG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 39
XbaI TCTAGA 1 cut(s) 23
XceI RCATGY 1 cut(s) 344
XspI CTAG 3 cut(s) 24, 68, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.