FvH4_4g00102

Retroviral aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001157
Physical Location & Seq
Forward (+)
126204 .. 127367
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00102.t1

Sequence Viewer

Length: 1164 bp
ATGCGTAGAACCATCAAACATTACATTGCCGCTTGTGATCAATGTCAGCGGCATTCCTATGAGACAATTCGGCCACCCGGACTACTTCAACCACTGCCGGTCCCGGATGAAGTTTTTACCCACATATCCATGGACTTCATTGACGGTTTACCGCAATCACAAGGTCACAACGCGATCATGGTCGTGGTTGATCGCCTCTCCAAATATGCTCACTTCATTCCGGTCTCCCATCCCTACTCAGCCTCCGGCATTGCTTCAATTTTCACTAAGGAGATTTTTAGGCTCCATGGCACACCTAAGAGGATTGTTAGTGATCGAGACCCCATCTTCATCAGTCATTTCTGGCGCACCTTCCATCGACTACAAGGAACACAGCTATGCCACAGCTCCGCTTATCATCCCCAATCTGATGGCCAAACCGAGGTCGTTAATCGATCCGTGGAGCACTACTTGCGCTGCTATGTAGCCGACAAGCCTTCTTCATGGGCTGAACTACTCCATTGGGCGGAATGGTGGTATAACACCACATTTCACTCCTCCATCCGTATGACACCGTTTGAAGCACTCTATGGCAAGCCTCCCCCGTCGATCACAAGGTATCTACCAGGTTCTACTGCTGTCCATAACACGGATGTGGCACTCCGCAGCCGTGATCAACTGCTATCCCAACTCAAACACAACTTGGAATTGGTCCGCAACCGAATGAAGCAGCAAGCTGATAAGCAACGGACTAAGAGAGAATTTCAGGTCGATGATTGGGTGTTTCTCAAGCTGCACCCCTACCGACAACAATCTGTGGTCAAGCGTCCTGCCCATAAGCTAGCACCACGCTTTTACGGTCCATTTCGGGTCCTCGAAAAGATTGGTTCAGTTGCATATCGCTTGGCTCTCCCAGCTTGCTCTAAGATCCACCCGACCTTCCATGTGTCACTCCTAAAAAAGAGGGTCGGCGATGGTGAACCGGTGTCCACTACACCGCCGCCGTTTGACGACCATGGAGAGTTACTGTGGACACCCGAGCGGGTACTTGACATGGCTGTACAACGCCGGATGAAGAGGAATGTCACCATGTGGCTTGTCCAATGGCAGGGACTTCCAGCTGAAGATGCCACTTGGGAAGTAGCTCACTTACTCCGGGCTAAGTTTCCCAACTTTGGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2

Protein Analysis

388

Amino Acids

44.86

Weight (kDa)

9.64

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 38 - 134 3.9e-08 Integrase core domain
SH3_Tf2-1 PF24626 252 - 314 4e-24 Tf2-1-like, SH3 domain
Chromo PF00385 338 - 383 3.4e-06 Chromo (CHRromatin Organisation MOdifier) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1021
AccII CGCG 1 cut(s) 173
AclWI GGATC 2 cut(s) 429, 901
AcoI YGGCCR 2 cut(s) 71, 412
AcsI RAATTY 1 cut(s) 740
AcuI CTGAAG 1 cut(s) 1122
AdeI CACNNNGTG 1 cut(s) 1071
AfaI GTAC 2 cut(s) 1026, 1041
AfiI CCNNNNNNNGG 5 cut(s) 421, 505, 628, 1087, 1154
AgeI ACCGGT 1 cut(s) 961
AgsI TTSAA 3 cut(s) 89, 258, 560
AjnI CCWGG 1 cut(s) 604
AleI CACNNNNGTG 1 cut(s) 632
AluBI AGCT 8 cut(s) 376, 387, 716, 772, 820, 896, 1100, 1124
AluI AGCT 8 cut(s) 376, 387, 716, 772, 820, 896, 1100, 1124
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 3 cut(s) 56, 229, 312
AlwI GGATC 2 cut(s) 429, 901
Ama87I CYCGRG 1 cut(s) 1016
AoxI GGCC 2 cut(s) 71, 412
ApeKI GCWGC 4 cut(s) 456, 645, 709, 772
ApoI RAATTY 1 cut(s) 740
AsiGI ACCGGT 1 cut(s) 961
AspLEI GCGC 3 cut(s) 348, 456, 1160
AspS9I GGNCC 4 cut(s) 100, 691, 839, 850
AsuC2I CCSGG 3 cut(s) 78, 104, 1136
AsuHPI GGTGA 2 cut(s) 968, 1057
AsuNHI GCTAGC 1 cut(s) 820
AvaI CYCGRG 1 cut(s) 1016
AvaII GGWCC 4 cut(s) 100, 691, 839, 850
BalI TGGCCA 1 cut(s) 414
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 4 cut(s) 443, 657, 721, 759
BccI CCATC 7 cut(s) 20, 237, 332, 363, 404, 548, 947
BceAI ACGGC 2 cut(s) 633, 967
BciT130I CCWGG 1 cut(s) 606
BclI TGATCA 2 cut(s) 37, 652
BcnI CCSGG 3 cut(s) 78, 104, 1136
BcoDI GTCTC 3 cut(s) 56, 229, 312
BfaI CTAG 1 cut(s) 821
BfoI RGCGCY 1 cut(s) 1161
BisI GCNGC 7 cut(s) 30, 50, 457, 646, 710, 773, 980
BlsI GCNGC 7 cut(s) 31, 51, 458, 647, 711, 774, 981
Bme1390I CCNGG 4 cut(s) 78, 104, 606, 1136
Bme18I GGWCC 4 cut(s) 100, 691, 839, 850
BmeT110I CYCGRG 1 cut(s) 1016
BmgT120I GGNCC 4 cut(s) 100, 691, 839, 850
BmiI GGNNCC 3 cut(s) 102, 284, 851
BmrFI CCNGG 4 cut(s) 78, 104, 606, 1136
BmsI GCATC 1 cut(s) 1096
BmtI GCTAGC 1 cut(s) 824
BpuEI CTTGAG 1 cut(s) 752
BpuMI CCSGG 3 cut(s) 78, 104, 1136
Bsa29I ATCGAT 1 cut(s) 433
BsaI GGTCTC 2 cut(s) 229, 312
BsaJI CCNNGG 5 cut(s) 129, 286, 420, 438, 994
BsaWI WCCGGW 2 cut(s) 220, 961
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 5 cut(s) 421, 505, 628, 1087, 1154
Bse118I RCCGGY 2 cut(s) 97, 961
Bse3DI GCAATG 2 cut(s) 24, 249
BseBI CCWGG 1 cut(s) 606
BseCI ATCGAT 1 cut(s) 433
BseDI CCNNGG 5 cut(s) 129, 286, 420, 438, 994
BseGI GGATG 6 cut(s) 112, 229, 397, 540, 637, 1056
BseLI CCNNNNNNNGG 5 cut(s) 421, 505, 628, 1087, 1154
BseMI GCAATG 2 cut(s) 24, 249
BseMII CTCAG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 526
BseXI GCAGC 4 cut(s) 443, 657, 721, 759
BseYI CCCAGC 1 cut(s) 892
BsgI GTGCAG 1 cut(s) 758
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 2 cut(s) 73, 414
BshTI ACCGGT 1 cut(s) 961
BshVI ATCGAT 1 cut(s) 433
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 1016
BsiSI CCGG 8 cut(s) 78, 98, 104, 221, 246, 962, 1048, 1135
BslFI GGGAC 2 cut(s) 86, 1104
BslI CCNNNNNNNGG 5 cut(s) 421, 505, 628, 1087, 1154
BsmAI GTCTC 3 cut(s) 56, 229, 312
BsmFI GGGAC 2 cut(s) 86, 1104
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 2 cut(s) 73, 414
Bso31I GGTCTC 2 cut(s) 229, 312
BsoBI CYCGRG 1 cut(s) 1016
Bsp1286I GDGCHC 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 1039
Bsp143I GATC 8 cut(s) 37, 174, 190, 313, 434, 588, 652, 906
Bsp19I CCATGG 3 cut(s) 129, 286, 994
BspANI GGCC 2 cut(s) 73, 414
BspCNI CTCAG 1 cut(s) 251
BspDI ATCGAT 1 cut(s) 433
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 3 cut(s) 102, 284, 851
BspOI GCTAGC 1 cut(s) 824
BspPI GGATC 2 cut(s) 429, 901
BspTNI GGTCTC 2 cut(s) 229, 312
BsrBI CCGCTC 1 cut(s) 1021
BsrDI GCAATG 2 cut(s) 24, 249
BsrFI RCCGGY 2 cut(s) 97, 961
BsrGI TGTACA 1 cut(s) 1039
BssAI RCCGGY 2 cut(s) 97, 961
BssECI CCNNGG 5 cut(s) 129, 286, 420, 438, 994
BssMI GATC 8 cut(s) 37, 174, 190, 313, 434, 588, 652, 906
BssT1I CCWWGG 3 cut(s) 129, 286, 994
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 4 cut(s) 146, 555, 839, 1008
Bst6I CTCTTC 1 cut(s) 1049
BstAPI GCANNNNNTGC 1 cut(s) 451
BstAUI TGTACA 1 cut(s) 1039
BstC8I GCNNGC 4 cut(s) 575, 714, 822, 898
BstDEI CTNAG 6 cut(s) 238, 267, 297, 732, 903, 1140
BstDSI CCRYGG 4 cut(s) 129, 286, 438, 994
BstF5I GGATG 6 cut(s) 112, 229, 397, 540, 637, 1056
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 1 cut(s) 1161
BstHHI GCGC 3 cut(s) 348, 456, 1160
BstKTI GATC 8 cut(s) 40, 177, 193, 316, 437, 591, 655, 909
BstMAI GTCTC 3 cut(s) 56, 229, 312
BstMBI GATC 8 cut(s) 37, 174, 190, 313, 434, 588, 652, 906
BstMWI GCNNNNNNNGC 3 cut(s) 451, 893, 1106
BstNI CCWGG 1 cut(s) 606
BstSCI CCNGG 4 cut(s) 76, 102, 604, 1134
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 4 cut(s) 443, 657, 721, 759
BstX2I RGATCY 1 cut(s) 906
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 1 cut(s) 906
Bsu15I ATCGAT 1 cut(s) 433
BsuRI GGCC 2 cut(s) 73, 414
BsuTUI ATCGAT 1 cut(s) 433
BtgI CCRYGG 4 cut(s) 129, 286, 438, 994
BtgZI GCGATG 1 cut(s) 966
BtsCI GGATG 6 cut(s) 112, 229, 397, 540, 637, 1056
BtsI GCAGTG 1 cut(s) 92
BtsIMutI CAGTG 1 cut(s) 92
Cac8I GCNNGC 4 cut(s) 575, 714, 822, 898
CfoI GCGC 3 cut(s) 348, 456, 1160
Cfr10I RCCGGY 2 cut(s) 97, 961
Cfr13I GGNCC 4 cut(s) 100, 691, 839, 850
ClaI ATCGAT 1 cut(s) 433
CseI GACGC 1 cut(s) 794
CsiI ACCWGGT 1 cut(s) 604
Csp6I GTAC 2 cut(s) 1025, 1040
CspAI ACCGGT 1 cut(s) 961
CviAII CATG 8 cut(s) 130, 178, 287, 483, 923, 995, 1033, 1069
CviQI GTAC 2 cut(s) 1025, 1040
DdeI CTNAG 6 cut(s) 238, 267, 297, 732, 903, 1140
DpnI GATC 8 cut(s) 39, 176, 192, 315, 436, 590, 654, 908
DpnII GATC 8 cut(s) 37, 174, 190, 313, 434, 588, 652, 906
DraIII CACNNNGTG 1 cut(s) 1071
EaeI YGGCCR 2 cut(s) 71, 412
Eam1104I CTCTTC 1 cut(s) 1049
EarI CTCTTC 1 cut(s) 1049
EciI GGCGGA 1 cut(s) 521
Eco130I CCWWGG 3 cut(s) 129, 286, 994
Eco31I GGTCTC 2 cut(s) 229, 312
Eco47I GGWCC 4 cut(s) 100, 691, 839, 850
Eco57I CTGAAG 1 cut(s) 1122
Eco88I CYCGRG 1 cut(s) 1016
EcoO109I RGGNCCY 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 604
EcoT14I CCWWGG 3 cut(s) 129, 286, 994
ErhI CCWWGG 3 cut(s) 129, 286, 994
FaeI CATG 8 cut(s) 133, 181, 290, 486, 926, 998, 1036, 1072
FaqI GGGAC 2 cut(s) 86, 1104
FatI CATG 8 cut(s) 129, 177, 286, 482, 922, 994, 1032, 1068
FauI CCCGC 1 cut(s) 1014
FbaI TGATCA 2 cut(s) 37, 652
Fnu4HI GCNGC 7 cut(s) 30, 50, 457, 646, 710, 773, 980
FokI GGATG 6 cut(s) 119, 216, 384, 527, 644, 1063
Fsp4HI GCNGC 7 cut(s) 30, 50, 457, 646, 710, 773, 980
FspBI CTAG 1 cut(s) 821
GlaI GCGC 3 cut(s) 347, 455, 1159
GluI GCNGC 7 cut(s) 30, 50, 457, 646, 710, 773, 980
GsaI CCCAGC 1 cut(s) 896
HaeII RGCGCY 1 cut(s) 1161
HaeIII GGCC 2 cut(s) 73, 414
HapII CCGG 8 cut(s) 78, 98, 104, 221, 246, 962, 1048, 1135
HgaI GACGC 1 cut(s) 794
HhaI GCGC 3 cut(s) 348, 456, 1160
Hin1II CATG 8 cut(s) 133, 181, 290, 486, 926, 998, 1036, 1072
Hin6I GCGC 3 cut(s) 346, 454, 1158
HinP1I GCGC 3 cut(s) 346, 454, 1158
HpaII CCGG 8 cut(s) 78, 98, 104, 221, 246, 962, 1048, 1135
HphI GGTGA 2 cut(s) 968, 1057
Hpy166II GTNNAC 4 cut(s) 149, 959, 969, 1011
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 4 cut(s) 149, 959, 969, 1011
Hpy99I CGWCG 1 cut(s) 589
HpyAV CCTTC 3 cut(s) 361, 486, 928
HpyCH4III ACNGT 4 cut(s) 146, 555, 839, 1008
HpyCH4V TGCA 2 cut(s) 775, 875
HpyF10VI GCNNNNNNNGC 3 cut(s) 451, 893, 1106
HpyF3I CTNAG 6 cut(s) 238, 267, 297, 732, 903, 1140
Hsp92II CATG 8 cut(s) 133, 181, 290, 486, 926, 998, 1036, 1072
HspAI GCGC 3 cut(s) 346, 454, 1158
Ksp22I TGATCA 2 cut(s) 37, 652
Kzo9I GATC 8 cut(s) 37, 174, 190, 313, 434, 588, 652, 906
LmnI GCTCC 3 cut(s) 288, 392, 442
Lsp1109I GCAGC 4 cut(s) 443, 657, 721, 759
LweI GCATC 1 cut(s) 1096
MabI ACCWGGT 1 cut(s) 604
MaeI CTAG 1 cut(s) 821
MaeIII GTNAC 4 cut(s) 164, 927, 1002, 1063
MalI GATC 8 cut(s) 39, 176, 192, 315, 436, 590, 654, 908
MbiI CCGCTC 1 cut(s) 1021
MboI GATC 8 cut(s) 37, 174, 190, 313, 434, 588, 652, 906
MboII GAAGA 4 cut(s) 319, 471, 1066, 1115
MflI RGATCY 1 cut(s) 906
MhlI GDGCHC 1 cut(s) 447
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 4 cut(s) 66, 258, 686, 740
MluNI TGGCCA 1 cut(s) 414
MnlI CCTC 9 cut(s) 206, 253, 294, 415, 547, 588, 863, 936, 1050
Mox20I TGGCCA 1 cut(s) 414
MscI TGGCCA 1 cut(s) 414
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 6 cut(s) 57, 128, 182, 376, 545, 632
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 2 cut(s) 49, 1100
MspI CCGG 8 cut(s) 78, 98, 104, 221, 246, 962, 1048, 1135
MspR9I CCNGG 4 cut(s) 78, 104, 606, 1136
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 1 cut(s) 606
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 451, 893, 1106
NciI CCSGG 3 cut(s) 78, 104, 1136
NcoI CCATGG 3 cut(s) 129, 286, 994
NdeII GATC 8 cut(s) 37, 174, 190, 313, 434, 588, 652, 906
NheI GCTAGC 1 cut(s) 820
NlaIII CATG 8 cut(s) 133, 181, 290, 486, 926, 998, 1036, 1072
NlaIV GGNNCC 3 cut(s) 102, 284, 851
NmuCI GTSAC 3 cut(s) 164, 927, 1063
OliI CACNNNNGTG 1 cut(s) 632
PctI GAATGC 1 cut(s) 52
PfoI TCCNGGA 1 cut(s) 102
PinAI ACCGGT 1 cut(s) 961
PkrI GCNGC 7 cut(s) 31, 51, 458, 647, 711, 774, 981
PpuMI RGGWCCY 1 cut(s) 850
Psp5II RGGWCCY 1 cut(s) 850
Psp6I CCWGG 1 cut(s) 604
PspFI CCCAGC 1 cut(s) 892
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 3 cut(s) 102, 284, 851
PspPI GGNCC 4 cut(s) 100, 691, 839, 850
PspPPI RGGWCCY 1 cut(s) 850
PsuI RGATCY 1 cut(s) 906
PvuII CAGCTG 1 cut(s) 1100
RsaI GTAC 2 cut(s) 1026, 1041
RsaNI GTAC 2 cut(s) 1025, 1040
RseI CAYNNNNRTG 6 cut(s) 57, 128, 182, 376, 545, 632
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 7 cut(s) 30, 50, 457, 646, 710, 773, 980
Sau3AI GATC 8 cut(s) 37, 174, 190, 313, 434, 588, 652, 906
Sau96I GGNCC 4 cut(s) 100, 691, 839, 850
ScrFI CCNGG 4 cut(s) 78, 104, 606, 1136
SduI GDGCHC 1 cut(s) 447
SexAI ACCWGGT 1 cut(s) 604
SfaNI GCATC 1 cut(s) 1096
SinI GGWCC 4 cut(s) 100, 691, 839, 850
SmiMI CAYNNNNRTG 6 cut(s) 57, 128, 182, 376, 545, 632
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
Sse9I AATT 4 cut(s) 66, 258, 686, 740
SspMI CTAG 1 cut(s) 821
StyD4I CCNGG 4 cut(s) 76, 102, 604, 1134
StyI CCWWGG 3 cut(s) 129, 286, 994
TaaI ACNGT 4 cut(s) 146, 555, 839, 1008
TaqI TCGA 6 cut(s) 316, 358, 433, 587, 750, 855
TasI AATT 4 cut(s) 66, 258, 686, 740
TatI WGTACW 1 cut(s) 1039
TauI GCSGC 3 cut(s) 32, 52, 982
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 1 cut(s) 99
TseFI GTSAC 3 cut(s) 164, 927, 1063
TseI GCWGC 4 cut(s) 456, 645, 709, 772
Tsp45I GTSAC 3 cut(s) 164, 927, 1063
TspDTI ATGAA 7 cut(s) 123, 127, 205, 319, 471, 719, 1067
TspGWI ACGGA 4 cut(s) 427, 533, 644, 742
TspRI CASTG 1 cut(s) 99
VpaK11BI GGWCC 4 cut(s) 100, 691, 839, 850
XapI RAATTY 1 cut(s) 740
XspI CTAG 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.