RchiOBHm_Chr6g0290351

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001157
Physical Location & Seq
Reverse (-)
53411465 .. 53413323
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26049

Sequence Viewer

Length: 1272 bp
ATGTTTGCTATTGAGAAGTGGCGCCCCTACCTATTAGGTAATTTCTTCACTATTCTCATAGATCATCAAACTCTTAAACATCTTCTTGATCAACGAATCTCCACACCTTCTCAACACAAATGGTTGTCCAAGTTGTTAGGGTATAATTACACAATTGAGGACAAGCCTGGCAAAGCCAATACAGTTCCGGATTTGCTATCCCGCCGCCACGAAGTTATGGCTATTCAATCTGTCTCTGCCCCAATTTTCGATGGAATCCAATTAATTGATCGAGCTTGCCTTTCTGATCCTGAGGCTCAAGCTATTACTACAACAATATCCAATGGCACTCCAACAAAGAAAGGTTTCACAGTCGCCCAAGGCAGATTACTCTACAAAGATAGGATTTTTGTCCCTGCAACCTCAGATTGGCGTGCCAAAATACTTCATGAATTTCATTCCTCCTTTCAGGCAGGTCACTCAGGTTACTTACGCACTTTGATTAGAGTTTCATGCAACTTTGCTTGGCCCGGGATGCGGCGTGATGTCAAAACCTTCATAGCTGCTTGTGATCAGTGTCAACGACAGAACTATGAGTCCATACACCCTCCCGGCTTGTTGCAGCCTTTGCCAATTCCGGATGAGGTATATGTTGATATTACAATGGATTTTGTTGATGGTTTACCCATCTTTGATGGCTACAATGCTATTACGGTGGTTGTGGATCGTTTTTCGAAATATGCTCACTTTATTGCCATCACTCATCCTTACACTGCTTCACATATTGCTGATATTTTTATGAAAGAAGTCTTTCAACTACATGGTATGCCTCGCTCTATTGTCAATGACAAAGACCCCATATTCATAAGCCATTTTTGGACTGCCTTCTTCAAGTTACAAGGTACACATTTGTGCAGAAGTTCAGCCTATCATCCTGAGTCTGATGGTCAGTCAGAAGTAGTGAACAGATCCCTTGAGCATTATTTACGTTGCTTTGTATTTGACAAGCCCTCTTCATGGAGTTCCTTCCTGCATTGGGCCGAATGGTGGAATAACACCACTTTTCATTCCACCATCAAGATGTCACCCTTCCAGGCTTTATATGGCCGACCTCCACCTTCGTTGTCAACTTACTTGCCCGGCACAACTAAGGTGCATGCAGTGGACACTTCCTTGCAAGCCAGAGATCAACTGTTGAAGGATCTAAAGGATCATATGGCAAATGCGCAGAACAGAATGAAGCAGCACACTGATCAGCGAAGGACAGAGAGAGAGTTTAATGAGGGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

423

Amino Acids

48.63

Weight (kDa)

8.14

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH PF17917 1 - 48 7.3e-08 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 136 - 190 1.4e-17 Integrase zinc binding domain
rve PF00665 214 - 305 1.1e-06 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1206
Acc36I ACCTGC 1 cut(s) 443
AccB1I GGYRCC 1 cut(s) 21
AccIII TCCGGA 2 cut(s) 187, 616
AciI CCGC 3 cut(s) 202, 205, 517
AclWI GGATC 5 cut(s) 281, 711, 942, 1188, 1197
AcoI YGGCCR 1 cut(s) 1084
AcsI RAATTY 1 cut(s) 431
AcyI GRCGYC 1 cut(s) 22
AfaI GTAC 1 cut(s) 883
AfiI CCNNNNNNNGG 5 cut(s) 408, 516, 996, 1015, 1026
AgsI TTSAA 4 cut(s) 227, 794, 871, 1177
AjnI CCWGG 2 cut(s) 166, 1071
AleI CACNNNNGTG 1 cut(s) 889
AloI GAACNNNNNNTCC 2 cut(s) 560, 592
AluBI AGCT 3 cut(s) 275, 302, 542
AluI AGCT 3 cut(s) 275, 302, 542
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 5 cut(s) 281, 711, 942, 1188, 1197
Ama87I CYCGRG 1 cut(s) 509
Aor13HI TCCGGA 2 cut(s) 187, 616
AoxI GGCC 3 cut(s) 506, 1017, 1084
ApeKI GCWGC 3 cut(s) 542, 601, 1222
ApoI RAATTY 1 cut(s) 431
AseI ATTAAT 1 cut(s) 263
Asp700I GAANNNNTTC 2 cut(s) 344, 789
AspLEI GCGC 2 cut(s) 24, 1207
AspS9I GGNCC 2 cut(s) 507, 1017
AsuC2I CCSGG 4 cut(s) 510, 511, 591, 1119
AsuHPI GGTGA 1 cut(s) 1056
AsuII TTCGAA 1 cut(s) 713
AvaI CYCGRG 1 cut(s) 509
AxyI CCTNAGG 1 cut(s) 291
BanI GGYRCC 1 cut(s) 21
BbvI GCAGC 3 cut(s) 529, 613, 1234
BccI CCATC 7 cut(s) 245, 650, 668, 674, 743, 917, 1061
BciT130I CCWGG 2 cut(s) 168, 1073
BclI TGATCA 3 cut(s) 88, 550, 1231
BcnI CCSGG 4 cut(s) 510, 511, 591, 1119
BcoDI GTCTC 1 cut(s) 238
BfoI RGCGCY 1 cut(s) 25
BfuAI ACCTGC 1 cut(s) 443
BisI GCNGC 5 cut(s) 205, 518, 543, 602, 1223
BlsI GCNGC 5 cut(s) 206, 519, 544, 603, 1224
Bme1390I CCNGG 6 cut(s) 168, 510, 511, 591, 1073, 1119
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 2 cut(s) 507, 1017
BmiI GGNNCC 1 cut(s) 23
BmrFI CCNGG 6 cut(s) 168, 510, 511, 591, 1073, 1119
BmsI GCATC 1 cut(s) 504
Bpu14I TTCGAA 1 cut(s) 713
BpuEI CTTGAG 2 cut(s) 282, 974
BpuMI CCSGG 4 cut(s) 510, 511, 591, 1119
BsaHI GRCGYC 1 cut(s) 22
BsaJI CCNNGG 2 cut(s) 358, 509
BsaWI WCCGGW 2 cut(s) 187, 616
Bsc4I CCNNNNNNNGG 5 cut(s) 408, 516, 996, 1015, 1026
Bse21I CCTNAGG 1 cut(s) 291
BseAI TCCGGA 2 cut(s) 187, 616
BseBI CCWGG 2 cut(s) 168, 1073
BseDI CCNNGG 2 cut(s) 358, 509
BseGI GGATG 4 cut(s) 519, 625, 742, 910
BseLI CCNNNNNNNGG 5 cut(s) 408, 516, 996, 1015, 1026
BseMII CTCAG 4 cut(s) 282, 417, 474, 906
BseXI GCAGC 3 cut(s) 529, 613, 1234
BsgI GTGCAG 1 cut(s) 913
BshFI GGCC 3 cut(s) 508, 1019, 1086
BshNI GGYRCC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 5 cut(s) 188, 510, 591, 617, 1119
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 5 cut(s) 408, 516, 996, 1015, 1026
BsmAI GTCTC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 3 cut(s) 508, 1019, 1086
BsoBI CYCGRG 1 cut(s) 509
Bsp119I TTCGAA 1 cut(s) 713
Bsp13I TCCGGA 2 cut(s) 187, 616
BspACI CCGC 3 cut(s) 202, 205, 517
BspANI GGCC 3 cut(s) 508, 1019, 1086
BspCNI CTCAG 4 cut(s) 283, 416, 473, 907
BspEI TCCGGA 2 cut(s) 187, 616
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 1 cut(s) 23
BspMI ACCTGC 1 cut(s) 443
BspPI GGATC 5 cut(s) 281, 711, 942, 1188, 1197
BspT104I TTCGAA 1 cut(s) 713
BspT107I GGYRCC 1 cut(s) 21
BssECI CCNNGG 2 cut(s) 358, 509
BssNI GRCGYC 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 358
Bst2UI CCWGG 2 cut(s) 168, 1073
Bst4CI ACNGT 4 cut(s) 184, 352, 694, 1173
Bst6I CTCTTC 1 cut(s) 997
BstACI GRCGYC 1 cut(s) 22
BstAPI GCANNNNNTGC 1 cut(s) 607
BstBI TTCGAA 1 cut(s) 713
BstC8I GCNNGC 4 cut(s) 277, 414, 1137, 1158
BstDEI CTNAG 5 cut(s) 291, 403, 460, 915, 1128
BstF5I GGATG 4 cut(s) 519, 625, 742, 910
BstH2I RGCGCY 1 cut(s) 25
BstHHI GCGC 2 cut(s) 24, 1207
BstMAI GTCTC 1 cut(s) 238
BstMWI GCNNNNNNNGC 2 cut(s) 514, 607
BstNI CCWGG 2 cut(s) 168, 1073
BstNSI RCATGY 1 cut(s) 1139
BstSCI CCNGG 6 cut(s) 166, 508, 509, 589, 1071, 1117
BstV1I GCAGC 3 cut(s) 529, 613, 1234
BstX2I RGATCY 2 cut(s) 947, 1180
BstYI RGATCY 2 cut(s) 947, 1180
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 3 cut(s) 508, 1019, 1086
BtsCI GGATG 4 cut(s) 519, 625, 742, 910
BtsI GCAGTG 2 cut(s) 750, 1146
BtsIMutI CAGTG 4 cut(s) 560, 750, 1146, 1227
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 4 cut(s) 277, 414, 1137, 1158
CciI TCATGA 1 cut(s) 427
CfoI GCGC 2 cut(s) 24, 1207
Cfr13I GGNCC 2 cut(s) 507, 1017
Cfr9I CCCGGG 1 cut(s) 509
Csp6I GTAC 1 cut(s) 882
CspCI CAANNNNNGTGG 2 cut(s) 1083, 1118
CviAII CATG 5 cut(s) 428, 492, 800, 996, 1136
CviQI GTAC 1 cut(s) 882
DdeI CTNAG 5 cut(s) 291, 403, 460, 915, 1128
DinI GGCGCC 1 cut(s) 23
EaeI YGGCCR 1 cut(s) 1084
Eam1104I CTCTTC 1 cut(s) 997
EarI CTCTTC 1 cut(s) 997
Eco130I CCWWGG 1 cut(s) 358
Eco81I CCTNAGG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 509
EcoRII CCWGG 2 cut(s) 166, 1071
EcoT14I CCWWGG 1 cut(s) 358
EgeI GGCGCC 1 cut(s) 23
EheI GGCGCC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 358
FaeI CATG 5 cut(s) 431, 495, 803, 999, 1139
FaqI GGGAC 1 cut(s) 377
FatI CATG 5 cut(s) 427, 491, 799, 995, 1135
FauI CCCGC 1 cut(s) 209
FauNDI CATATG 1 cut(s) 1194
FbaI TGATCA 3 cut(s) 88, 550, 1231
Fnu4HI GCNGC 5 cut(s) 205, 518, 543, 602, 1223
FokI GGATG 4 cut(s) 526, 632, 729, 897
Fsp4HI GCNGC 5 cut(s) 205, 518, 543, 602, 1223
FspI TGCGCA 1 cut(s) 1206
GlaI GCGC 2 cut(s) 23, 1206
GluI GCNGC 5 cut(s) 205, 518, 543, 602, 1223
HaeII RGCGCY 1 cut(s) 25
HaeIII GGCC 3 cut(s) 508, 1019, 1086
HapII CCGG 5 cut(s) 188, 510, 591, 617, 1119
HhaI GCGC 2 cut(s) 24, 1207
Hin1I GRCGYC 1 cut(s) 22
Hin1II CATG 5 cut(s) 431, 495, 803, 999, 1139
Hin6I GCGC 2 cut(s) 22, 1205
HinP1I GCGC 2 cut(s) 22, 1205
HincII GTYRAC 2 cut(s) 560, 1107
HindII GTYRAC 2 cut(s) 560, 1107
HinfI GANTC 4 cut(s) 96, 255, 575, 917
HpaII CCGG 5 cut(s) 188, 510, 591, 617, 1119
HphI GGTGA 1 cut(s) 1056
Hpy166II GTNNAC 6 cut(s) 560, 662, 884, 943, 1107, 1144
Hpy188I TCNGA 4 cut(s) 286, 406, 922, 934
Hpy188III TCNNGA 7 cut(s) 86, 188, 290, 428, 617, 914, 1057
Hpy8I GTNNAC 6 cut(s) 560, 662, 884, 943, 1107, 1144
HpyAV CCTTC 8 cut(s) 117, 544, 874, 1015, 1078, 1107, 1171, 1233
HpyCH4III ACNGT 4 cut(s) 184, 352, 694, 1173
HpyCH4IV ACGT 1 cut(s) 967
HpyCH4V TGCA 8 cut(s) 398, 495, 601, 894, 1012, 1135, 1139, 1156
HpyF10VI GCNNNNNNNGC 2 cut(s) 514, 607
HpyF3I CTNAG 5 cut(s) 291, 403, 460, 915, 1128
HpySE526I ACGT 1 cut(s) 967
Hsp92I GRCGYC 1 cut(s) 22
Hsp92II CATG 5 cut(s) 431, 495, 803, 999, 1139
HspAI GCGC 2 cut(s) 22, 1205
KasI GGCGCC 1 cut(s) 21
Kpn2I TCCGGA 2 cut(s) 187, 616
Ksp22I TGATCA 3 cut(s) 88, 550, 1231
Lsp1109I GCAGC 3 cut(s) 529, 613, 1234
LweI GCATC 1 cut(s) 504
MaeII ACGT 1 cut(s) 967
MaeIII GTNAC 4 cut(s) 455, 464, 873, 1062
MboII GAAGA 4 cut(s) 37, 74, 859, 984
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 2 cut(s) 947, 1180
MluCI AATT 8 cut(s) 40, 145, 153, 243, 260, 264, 431, 612
Mly113I GGCGCC 1 cut(s) 22
MlyI GAGTC 2 cut(s) 584, 926
MmeI TCCRAC 1 cut(s) 356
MroI TCCGGA 2 cut(s) 187, 616
MroXI GAANNNNTTC 2 cut(s) 344, 789
MseI TTAA 3 cut(s) 75, 263, 1257
MslI CAYNNNNRTG 2 cut(s) 889, 1058
MspI CCGG 5 cut(s) 188, 510, 591, 617, 1119
MspR9I CCNGG 6 cut(s) 168, 510, 511, 591, 1073, 1119
MunI CAATTG 1 cut(s) 153
MvaI CCWGG 2 cut(s) 168, 1073
MwoI GCNNNNNNNGC 2 cut(s) 514, 607
NarI GGCGCC 1 cut(s) 22
NciI CCSGG 4 cut(s) 510, 511, 591, 1119
NdeI CATATG 1 cut(s) 1194
NlaIII CATG 5 cut(s) 431, 495, 803, 999, 1139
NlaIV GGNNCC 1 cut(s) 23
NmuCI GTSAC 2 cut(s) 455, 1062
NsbI TGCGCA 1 cut(s) 1206
NspI RCATGY 1 cut(s) 1139
NspV TTCGAA 1 cut(s) 713
OliI CACNNNNGTG 1 cut(s) 889
PaeI GCATGC 1 cut(s) 1139
PagI TCATGA 1 cut(s) 427
PdmI GAANNNNTTC 2 cut(s) 344, 789
PfeI GAWTC 2 cut(s) 96, 255
PkrI GCNGC 5 cut(s) 206, 519, 544, 603, 1224
PleI GAGTC 2 cut(s) 583, 925
PluTI GGCGCC 1 cut(s) 25
PpsI GAGTC 2 cut(s) 583, 925
PshBI ATTAAT 1 cut(s) 263
Psp6I CCWGG 2 cut(s) 166, 1071
PspGI CCWGG 2 cut(s) 166, 1071
PspN4I GGNNCC 1 cut(s) 23
PspPI GGNCC 2 cut(s) 507, 1017
PsuI RGATCY 2 cut(s) 947, 1180
RsaI GTAC 1 cut(s) 883
RsaNI GTAC 1 cut(s) 882
RseI CAYNNNNRTG 2 cut(s) 889, 1058
SaqAI TTAA 3 cut(s) 75, 263, 1257
SatI GCNGC 5 cut(s) 205, 518, 543, 602, 1223
Sau96I GGNCC 2 cut(s) 507, 1017
SchI GAGTC 2 cut(s) 584, 926
ScrFI CCNGG 6 cut(s) 168, 510, 511, 591, 1073, 1119
SfaNI GCATC 1 cut(s) 504
SfoI GGCGCC 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 713
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 2 cut(s) 889, 1058
SmlI CTYRAG 2 cut(s) 297, 953
SmoI CTYRAG 2 cut(s) 297, 953
SphI GCATGC 1 cut(s) 1139
Sse9I AATT 8 cut(s) 40, 145, 153, 243, 260, 264, 431, 612
SsiI CCGC 3 cut(s) 202, 205, 517
SspDI GGCGCC 1 cut(s) 21
StyD4I CCNGG 6 cut(s) 166, 508, 509, 589, 1071, 1117
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 4 cut(s) 184, 352, 694, 1173
TaiI ACGT 1 cut(s) 970
TaqI TCGA 3 cut(s) 249, 271, 713
TasI AATT 8 cut(s) 40, 145, 153, 243, 260, 264, 431, 612
TauI GCSGC 2 cut(s) 207, 520
TfiI GAWTC 2 cut(s) 96, 255
Tru1I TTAA 3 cut(s) 75, 263, 1257
Tru9I TTAA 3 cut(s) 75, 263, 1257
TscAI CASTG 4 cut(s) 560, 757, 1146, 1234
TseFI GTSAC 2 cut(s) 455, 1062
TseI GCWGC 3 cut(s) 542, 601, 1222
Tsp45I GTSAC 2 cut(s) 455, 1062
TspMI CCCGGG 1 cut(s) 509
TspRI CASTG 4 cut(s) 560, 757, 1146, 1234
VspI ATTAAT 1 cut(s) 263
XapI RAATTY 1 cut(s) 431
XceI RCATGY 1 cut(s) 1139
XcmI CCANNNNNNNNNTGG 1 cut(s) 1079
XmaI CCCGGG 1 cut(s) 509
XmnI GAANNNNTTC 2 cut(s) 344, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.