RchiOBHm_Chr5g0056131

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001157
Physical Location & Seq
Forward (+)
59523296 .. 59525238
1943 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33306

Sequence Viewer

Length: 942 bp
ATGATTGCCATGATGTTTGCAATAGACAAGTGGAGGCCGTATTTACTTGGTAACCCTTTTACAATTCGAACAAATCATCAGACTTTAAAGTATCTGCTGGACCAGAGGATATCAACACCACAACAACATAAATGGGTGGCAAAGCTGACGGGGTACAATTATACAATCGAGTACAAGCCCGGCAAACAAAATACAATACCTGATCTTCTATCCAGACGACATGAGCTCCTAGCTATCCAAACCGTTTCAGCCCCAATTTTTGATAGTCTACAGCTAATTGACAGAGCATGCTTGAATGACCCTGAAGCTCAAGGTATTATGCAGGCACTCATAAAGGGTACAACCACCAGGAAGAATTTCACTCTGCAAAATGGTCGACTTCTATATAAAGGTCGTATCTTTGTGCCGGAAACATCTGATTGGCGTTCAAAGATCTTGCACGAATTGCATGCTTCCCTTCAAGCCGGACATTCTGGATACCTTCGAACACTTGCAAGATTAGCTAAGAACTTTGCTTGGCCCGGATTACGACGAGATGTCAAGCTCTTCGTCTCCTCTTGTGATCAGTGCCAACGCCAAAATTATGAGTCAATTCATCCACCAGGGTTGCTTCAACCACTCCCAATTCCTGAAGAAACTTGGGAAGACGTCTCCATGGACTTCGTCGAAGGACTGCCAACTTCTCAGGGCTATAACTCAATCATGGTGGTGGTCGATCGCTTGTCTAAATTTGCCCACTTTATCCCAGTTGCTCATCCTTTTACTGCCCCCCAAATTGTTGATACATTCATGAATGAGGTGTTTCGACTTCATGGAATGCCCAAAAGAATTGTCAGTGATCGAGACCCTATATTCCTCAATCAGTTCTGGACTTCCTTCTTCAAGCTTCAAGGTACAAACTTATGCCATAGATCCGCATACCATCCACAGTCTGACGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

313

Amino Acids

36.07

Weight (kDa)

9.31

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH PF17917 1 - 52 7.3e-11 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 140 - 194 2.3e-18 Integrase zinc binding domain
rve PF00665 217 - 309 2.4e-06 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 651
AccI GTMKAC 2 cut(s) 268, 376
AciI CCGC 1 cut(s) 915
AclWI GGATC 1 cut(s) 906
AcsI RAATTY 2 cut(s) 355, 728
AcuI CTGAAG 2 cut(s) 324, 651
AcyI GRCGYC 1 cut(s) 648
AfaI GTAC 4 cut(s) 155, 173, 340, 895
AgsI TTSAA 6 cut(s) 295, 429, 461, 614, 883, 890
AhdI GACNNNNNGTC 1 cut(s) 536
AjnI CCWGG 2 cut(s) 347, 601
AluBI AGCT 8 cut(s) 145, 226, 233, 274, 308, 503, 544, 886
AluI AGCT 8 cut(s) 145, 226, 233, 274, 308, 503, 544, 886
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 3 cut(s) 556, 655, 837
AlwI GGATC 1 cut(s) 906
AoxI GGCC 2 cut(s) 35, 518
ApoI RAATTY 2 cut(s) 355, 728
ArsI GACNNNNNNTTYG 2 cut(s) 816, 848
Asp700I GAANNNNTTC 1 cut(s) 356
AspS9I GGNCC 2 cut(s) 100, 519
AsuC2I CCSGG 2 cut(s) 180, 522
AsuII TTCGAA 2 cut(s) 67, 484
AvaII GGWCC 1 cut(s) 100
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 651
Bbv12I GWGCWC 1 cut(s) 228
BccI CCATC 1 cut(s) 930
BceAI ACGGC 1 cut(s) 22
BcgI CGANNNNNNTGC 4 cut(s) 356, 390, 431, 465
BciT130I CCWGG 2 cut(s) 349, 603
BciVI GTATCC 1 cut(s) 470
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 180, 522
BcoDI GTCTC 3 cut(s) 556, 655, 837
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 269
BfuI GTATCC 1 cut(s) 470
BglII AGATCT 1 cut(s) 432
Bme1390I CCNGG 4 cut(s) 180, 349, 522, 603
Bme18I GGWCC 1 cut(s) 100
BmeRI GACNNNNNGTC 1 cut(s) 536
BmgT120I GGNCC 2 cut(s) 100, 519
BmrFI CCNGG 4 cut(s) 180, 349, 522, 603
BmrI ACTGGG 1 cut(s) 740
BmuI ACTGGG 1 cut(s) 740
BpiI GAAGAC 1 cut(s) 651
Bpu14I TTCGAA 2 cut(s) 67, 484
BpuEI CTTGAG 1 cut(s) 294
BpuMI CCSGG 2 cut(s) 180, 522
BsaHI GRCGYC 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 837
BsaJI CCNNGG 2 cut(s) 602, 654
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bse1I ACTGG 1 cut(s) 746
BseBI CCWGG 2 cut(s) 349, 603
BseDI CCNNGG 2 cut(s) 602, 654
BseGI GGATG 3 cut(s) 595, 754, 922
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 1 cut(s) 544
Bsh1285I CGRYCG 1 cut(s) 718
BshFI GGCC 2 cut(s) 37, 520
BsiEI CGRYCG 1 cut(s) 718
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 4 cut(s) 180, 407, 465, 522
BsmAI GTCTC 3 cut(s) 556, 655, 837
BsmBI CGTCTC 2 cut(s) 556, 655
BsmI GAATGC 1 cut(s) 822
BsnI GGCC 2 cut(s) 37, 520
Bso31I GGTCTC 1 cut(s) 837
Bsp119I TTCGAA 2 cut(s) 67, 484
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 6 cut(s) 202, 432, 562, 715, 838, 911
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 1 cut(s) 915
BspANI GGCC 2 cut(s) 37, 520
BspCNI CTCAG 1 cut(s) 697
BspHI TCATGA 1 cut(s) 789
BspPI GGATC 1 cut(s) 906
BspQI GCTCTTC 1 cut(s) 551
BspT104I TTCGAA 2 cut(s) 67, 484
BspTNI GGTCTC 1 cut(s) 837
BsrI ACTGG 1 cut(s) 746
BssECI CCNNGG 2 cut(s) 602, 654
BssMI GATC 6 cut(s) 202, 432, 562, 715, 838, 911
BssNI GRCGYC 1 cut(s) 648
BssT1I CCWWGG 1 cut(s) 654
Bst2UI CCWGG 2 cut(s) 349, 603
Bst4CI ACNGT 3 cut(s) 244, 930, 938
Bst6I CTCTTC 1 cut(s) 551
BstACI GRCGYC 1 cut(s) 648
BstAPI GCANNNNNTGC 1 cut(s) 445
BstBI TTCGAA 2 cut(s) 67, 484
BstC8I GCNNGC 3 cut(s) 289, 324, 450
BstDEI CTNAG 2 cut(s) 504, 684
BstDSI CCRYGG 1 cut(s) 654
BstEII GGTNACC 1 cut(s) 50
BstF5I GGATG 3 cut(s) 595, 754, 922
BstKTI GATC 6 cut(s) 205, 435, 565, 718, 841, 914
BstMAI GTCTC 3 cut(s) 556, 655, 837
BstMBI GATC 6 cut(s) 202, 432, 562, 715, 838, 911
BstMCI CGRYCG 1 cut(s) 718
BstMWI GCNNNNNNNGC 2 cut(s) 445, 500
BstNI CCWGG 2 cut(s) 349, 603
BstNSI RCATGY 2 cut(s) 291, 452
BstPI GGTNACC 1 cut(s) 50
BstSCI CCNGG 4 cut(s) 178, 347, 520, 601
BstSFI CTRYAG 1 cut(s) 269
BstV2I GAAGAC 1 cut(s) 651
BstX2I RGATCY 2 cut(s) 432, 911
BstYI RGATCY 2 cut(s) 432, 911
BsuI GTATCC 1 cut(s) 470
BsuRI GGCC 2 cut(s) 37, 520
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 3 cut(s) 595, 754, 922
BtsIMutI CAGTG 2 cut(s) 572, 841
Cac8I GCNNGC 3 cut(s) 289, 324, 450
CciI TCATGA 1 cut(s) 789
Cfr13I GGNCC 2 cut(s) 100, 519
Csp6I GTAC 4 cut(s) 154, 172, 339, 894
CspCI CAANNNNNGTGG 4 cut(s) 588, 623, 687, 722
CviAII CATG 8 cut(s) 10, 221, 288, 449, 655, 703, 790, 812
CviQI GTAC 4 cut(s) 154, 172, 339, 894
DdeI CTNAG 2 cut(s) 504, 684
DpnI GATC 6 cut(s) 204, 434, 564, 717, 840, 913
DpnII GATC 6 cut(s) 202, 432, 562, 715, 838, 911
DraI TTTAAA 1 cut(s) 87
DriI GACNNNNNGTC 1 cut(s) 536
Eam1104I CTCTTC 1 cut(s) 551
Eam1105I GACNNNNNGTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 551
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 654
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 837
Eco32I GATATC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 100
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 324, 651
Eco91I GGTNACC 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 50
EcoRII CCWGG 2 cut(s) 347, 601
EcoRV GATATC 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 654
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 654
Esp3I CGTCTC 2 cut(s) 556, 655
FaeI CATG 8 cut(s) 13, 224, 291, 452, 658, 706, 793, 815
FatI CATG 8 cut(s) 9, 220, 287, 448, 654, 702, 789, 811
FbaI TGATCA 1 cut(s) 562
FblI GTMKAC 2 cut(s) 268, 376
FokI GGATG 3 cut(s) 582, 741, 909
FriOI GRGCYC 1 cut(s) 228
FspBI CTAG 1 cut(s) 230
HaeIII GGCC 2 cut(s) 37, 520
HapII CCGG 4 cut(s) 180, 407, 465, 522
Hin1I GRCGYC 1 cut(s) 648
Hin1II CATG 8 cut(s) 13, 224, 291, 452, 658, 706, 793, 815
HincII GTYRAC 1 cut(s) 377
HindII GTYRAC 1 cut(s) 377
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 1 cut(s) 587
HpaII CCGG 4 cut(s) 180, 407, 465, 522
Hpy166II GTNNAC 2 cut(s) 269, 377
Hpy188I TCNGA 3 cut(s) 81, 418, 934
Hpy188III TCNNGA 6 cut(s) 213, 474, 629, 790, 842, 868
Hpy8I GTNNAC 2 cut(s) 269, 377
Hpy99I CGWCG 2 cut(s) 534, 668
HpyAV CCTTC 4 cut(s) 467, 491, 662, 886
HpyCH4III ACNGT 3 cut(s) 244, 930, 938
HpyCH4IV ACGT 1 cut(s) 648
HpyCH4V TGCA 6 cut(s) 20, 322, 367, 439, 448, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 500
HpyF3I CTNAG 2 cut(s) 504, 684
HpySE526I ACGT 1 cut(s) 648
Hsp92I GRCGYC 1 cut(s) 648
Hsp92II CATG 8 cut(s) 13, 224, 291, 452, 658, 706, 793, 815
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 6 cut(s) 202, 432, 562, 715, 838, 911
LguI GCTCTTC 1 cut(s) 551
LmnI GCTCC 1 cut(s) 231
MaeI CTAG 1 cut(s) 230
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 1 cut(s) 50
MalI GATC 6 cut(s) 204, 434, 564, 717, 840, 913
MboI GATC 6 cut(s) 202, 432, 562, 715, 838, 911
MboII GAAGA 6 cut(s) 197, 364, 538, 644, 656, 871
MflI RGATCY 2 cut(s) 432, 911
MhlI GDGCHC 1 cut(s) 228
MlyI GAGTC 1 cut(s) 596
MnlI CCTC 5 cut(s) 27, 99, 565, 790, 866
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 2 cut(s) 86, 940
MslI CAYNNNNRTG 1 cut(s) 707
MspI CCGG 4 cut(s) 180, 407, 465, 522
MspR9I CCNGG 4 cut(s) 180, 349, 522, 603
Mva1269I GAATGC 1 cut(s) 822
MvaI CCWGG 2 cut(s) 349, 603
MwoI GCNNNNNNNGC 2 cut(s) 445, 500
NciI CCSGG 2 cut(s) 180, 522
NcoI CCATGG 1 cut(s) 654
NdeII GATC 6 cut(s) 202, 432, 562, 715, 838, 911
NlaIII CATG 8 cut(s) 13, 224, 291, 452, 658, 706, 793, 815
NspI RCATGY 2 cut(s) 291, 452
NspV TTCGAA 2 cut(s) 67, 484
PaeI GCATGC 2 cut(s) 291, 452
PagI TCATGA 1 cut(s) 789
PciSI GCTCTTC 1 cut(s) 551
PctI GAATGC 1 cut(s) 822
PdmI GAANNNNTTC 1 cut(s) 356
PflFI GACNNNGTC 1 cut(s) 662
Ple19I CGATCG 1 cut(s) 718
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 2 cut(s) 347, 601
PspEI GGTNACC 1 cut(s) 50
PspGI CCWGG 2 cut(s) 347, 601
PspPI GGNCC 2 cut(s) 100, 519
PsuI RGATCY 2 cut(s) 432, 911
PsyI GACNNNGTC 1 cut(s) 662
PvuI CGATCG 1 cut(s) 718
RsaI GTAC 4 cut(s) 155, 173, 340, 895
RsaNI GTAC 4 cut(s) 154, 172, 339, 894
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 1 cut(s) 375
SapI GCTCTTC 1 cut(s) 551
SaqAI TTAA 2 cut(s) 86, 940
Sau3AI GATC 6 cut(s) 202, 432, 562, 715, 838, 911
Sau96I GGNCC 2 cut(s) 100, 519
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 4 cut(s) 180, 349, 522, 603
SduI GDGCHC 1 cut(s) 228
SfcI CTRYAG 1 cut(s) 269
SfuI TTCGAA 2 cut(s) 67, 484
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
SphI GCATGC 2 cut(s) 291, 452
SsiI CCGC 1 cut(s) 915
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 4 cut(s) 178, 347, 520, 601
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 3 cut(s) 244, 930, 938
TaiI ACGT 1 cut(s) 651
TaqI TCGA 8 cut(s) 67, 168, 376, 484, 666, 714, 805, 841
TatI WGTACW 1 cut(s) 171
Tru1I TTAA 2 cut(s) 86, 940
Tru9I TTAA 2 cut(s) 86, 940
TscAI CASTG 2 cut(s) 572, 841
TspDTI ATGAA 4 cut(s) 584, 778, 800, 806
TspRI CASTG 2 cut(s) 572, 841
Tth111I GACNNNGTC 1 cut(s) 662
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 2 cut(s) 355, 728
XceI RCATGY 2 cut(s) 291, 452
XmiI GTMKAC 2 cut(s) 268, 376
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 1 cut(s) 230
ZraI GACGTC 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.