FvH4_6g15081

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001157
Physical Location & Seq
Forward (+)
9364176 .. 9367157
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15081.t1

Sequence Viewer

Length: 1236 bp
ATGGCCATTCTGGAACCACCAGTTTCCGGGGAGGACCCCTCATCGGCTGAGTCATCGGAGGATGAACCCCCCACCGAGGTCGACACCGTCGTTCCTCTCCACGCCATCACCAATACTAAGCTTAGTGATATGATGCGCTTCAAGGGTTCGATTAATGGCAACATCATCGACGTCTTTGTCGACTGCGGCTCAGCGGTTAATTTCCTCCACCCTGCCGTCGCCGCACGATTGGACCTCCCGCGCACTACCGCGGAACCACTTAGGTTCACATCTGCCACCGGTCAGACAGCCAAACCTTCCGGCCTCGCATGCAGAGTAACAGTGAAAATACAGGAGTATGAGCTCACAGATGACTTCCTCCTCCTACCGGTGCCGGGCTGCGACCTTCTCCTCGGTGCTCAATGGTTGGACACCCTGGGTTTCATTGGCTGGCACTTCTTTGAGAAAGTGATGATGTTTGTGGCAAATGGCAAACACCATGTTCTTTATGGCATCAAGCAGCTCCCGGCAACTGAACCATCGCACAACTTGATGGCCATTCTCGCACAGGAGCAAATAGACTATGCCACCCTCGGGCCAACGGTCCCTCAAGACGAGGCCCACTGCCCCCAAGTCCAGCAACTGTTGACTGACTTTCACATGTTGTTTGAAGCCCCGATAGGTTTGCCACCAATACGGGCCATCGATCATCGCATCCCACTCCTCCCATCCGCTAGTCCAGTTAATGTTCGTCCTTACCGGTACCCCTATTCGCAAAAGGCAGAGCTCGAGACCCAAGTCAATGGGATGCTTTCCAATGGTCTTATCTGCCCTAGTCAAAGCCCTTTTTCTTCCCTGGTTTTACTAGTTTGGAAGAAGGATAAGGCATGGAGGTTTTGTGTGGATTACCGTGATCTCAACGCGGTCACCATCAAGGTTCGCTTCCCGATTCCGGTCGTGGACGAACTTTTAGATGAACTCCATGGTGCCCGATATTTTACCAAATTGGATCTCCGTGCCGGGTATCACCAAATTCGGATGTGTGAGCAAGACATTCACAAGACGGCATTCCGTACACACAACGGCCATTATGAATTCGTGGTTATGCCATTTGGTCTTTCTAATGCTCCTAGTACTTTTCAAGCTTTAATGAACCTTGTGTTCAAACCGCTTTTGCGCAAAGATCATAGAATGCATATATTGACACATATATTGTCAATGTCTGCATATATTGTGAGAAAATCTGAAAATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

412

Amino Acids

46.1

Weight (kDa)

6.09

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag-asp_proteas PF13975 48 - 136 9.8e-08 gag-polyprotein putative aspartyl protease
RVT_1 PF00078 285 - 387 1.9e-20 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AatII GACGTC 1 cut(s) 174
Acc16I TGCGCA 1 cut(s) 1157
Acc65I GGTACC 1 cut(s) 741
AccB1I GGYRCC 3 cut(s) 370, 741, 965
AccI GTMKAC 2 cut(s) 81, 180
AccII CGCG 3 cut(s) 241, 251, 902
AciI CCGC 9 cut(s) 186, 194, 222, 239, 249, 251, 711, 902, 1148
AclWI GGATC 1 cut(s) 996
AcoI YGGCCR 3 cut(s) 3, 534, 1063
AcsI RAATTY 2 cut(s) 1011, 1073
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 3 cut(s) 743, 1054, 1114
AfiI CCNNNNNNNGG 7 cut(s) 26, 43, 76, 367, 374, 573, 931
AflIII ACRYGT 1 cut(s) 639
AgeI ACCGGT 3 cut(s) 278, 367, 738
AgsI TTSAA 4 cut(s) 142, 650, 1121, 1144
AhlI ACTAGT 1 cut(s) 844
AjnI CCWGG 2 cut(s) 414, 834
AluBI AGCT 5 cut(s) 121, 343, 502, 766, 1124
AluI AGCT 5 cut(s) 121, 343, 502, 766, 1124
Alw21I GWGCWC 3 cut(s) 345, 400, 768
Alw26I GTCTC 1 cut(s) 764
AlwI GGATC 1 cut(s) 996
AlwNI CAGNNNCTG 1 cut(s) 622
Ama87I CYCGRG 2 cut(s) 572, 767
AoxI GGCC 7 cut(s) 3, 301, 534, 575, 597, 678, 1063
ApeKI GCWGC 2 cut(s) 378, 499
ApoI RAATTY 2 cut(s) 1011, 1073
AseI ATTAAT 1 cut(s) 153
AsiGI ACCGGT 3 cut(s) 278, 367, 738
Asp718I GGTACC 1 cut(s) 741
AspLEI GCGC 3 cut(s) 138, 243, 1158
AspS9I GGNCC 6 cut(s) 34, 232, 575, 583, 598, 678
AsuC2I CCSGG 4 cut(s) 28, 375, 506, 1000
AsuHPI GGTGA 3 cut(s) 100, 898, 998
AvaI CYCGRG 2 cut(s) 572, 767
AvaII GGWCC 3 cut(s) 34, 232, 583
BaeGI GKGCMC 1 cut(s) 970
BalI TGGCCA 2 cut(s) 5, 536
BanI GGYRCC 3 cut(s) 370, 741, 965
BanII GRGCYC 2 cut(s) 345, 768
Bbv12I GWGCWC 3 cut(s) 345, 400, 768
BbvI GCAGC 2 cut(s) 365, 511
BccI CCATC 6 cut(s) 113, 526, 526, 689, 715, 917
BceAI ACGGC 3 cut(s) 200, 1059, 1078
BcgI CGANNNNNNTGC 4 cut(s) 148, 182, 646, 680
BciT130I CCWGG 2 cut(s) 416, 836
BcnI CCSGG 4 cut(s) 28, 375, 506, 1000
BcoDI GTCTC 1 cut(s) 764
BcuI ACTAGT 1 cut(s) 844
BfaI CTAG 4 cut(s) 714, 813, 845, 1110
BisI GCNGC 4 cut(s) 187, 222, 379, 500
BlpI GCTNAGC 1 cut(s) 190
BlsI GCNGC 4 cut(s) 188, 223, 380, 501
BmcAI AGTACT 1 cut(s) 1114
Bme1390I CCNGG 6 cut(s) 28, 375, 416, 506, 836, 1000
Bme18I GGWCC 3 cut(s) 34, 232, 583
BmeT110I CYCGRG 2 cut(s) 572, 767
BmgT120I GGNCC 6 cut(s) 34, 232, 575, 583, 598, 678
BmiI GGNNCC 7 cut(s) 15, 36, 255, 372, 585, 743, 967
BmrFI CCNGG 6 cut(s) 28, 375, 416, 506, 836, 1000
BmsI GCATC 4 cut(s) 123, 501, 702, 777
BoxI GACNNNNGTC 1 cut(s) 776
BplI GAGNNNNNCTC 2 cut(s) 23, 55
Bpu1102I GCTNAGC 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 573
BpuMI CCSGG 4 cut(s) 28, 375, 506, 1000
Bsa29I ATCGAT 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 9 cut(s) 27, 75, 249, 391, 414, 415, 571, 834, 961
BsaWI WCCGGW 4 cut(s) 278, 367, 738, 931
BsaXI ACNNNNNCTCC 2 cut(s) 862, 892
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 43, 76, 367, 374, 573, 931
Bse118I RCCGGY 3 cut(s) 278, 367, 738
Bse1I ACTGG 2 cut(s) 20, 719
BseBI CCWGG 2 cut(s) 416, 836
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 9 cut(s) 27, 75, 249, 391, 414, 415, 571, 834, 961
BseGI GGATG 5 cut(s) 67, 693, 707, 792, 1023
BseLI CCNNNNNNNGG 7 cut(s) 26, 43, 76, 367, 374, 573, 931
BseMII CTCAG 2 cut(s) 39, 204
BseNI ACTGG 2 cut(s) 20, 719
BseRI GAGGAG 3 cut(s) 350, 380, 692
BseSI GKGCMC 1 cut(s) 970
BseXI GCAGC 2 cut(s) 365, 511
Bsh1236I CGCG 3 cut(s) 241, 251, 902
Bsh1285I CGRYCG 1 cut(s) 936
BshFI GGCC 7 cut(s) 5, 303, 536, 577, 599, 680, 1065
BshNI GGYRCC 3 cut(s) 370, 741, 965
BshTI ACCGGT 3 cut(s) 278, 367, 738
BshVI ATCGAT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 936
BsiHKAI GWGCWC 3 cut(s) 345, 400, 768
BsiHKCI CYCGRG 2 cut(s) 572, 767
BsiSI CCGG 9 cut(s) 27, 279, 300, 368, 374, 506, 739, 932, 999
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 7 cut(s) 26, 43, 76, 367, 374, 573, 931
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 569
BsmI GAATGC 2 cut(s) 1046, 1176
BsnI GGCC 7 cut(s) 5, 303, 536, 577, 599, 680, 1065
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 2 cut(s) 572, 767
Bsp1286I GDGCHC 4 cut(s) 345, 400, 768, 970
Bsp143I GATC 4 cut(s) 685, 892, 988, 1162
Bsp1720I GCTNAGC 1 cut(s) 190
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 9 cut(s) 186, 194, 222, 239, 249, 251, 711, 902, 1148
BspANI GGCC 7 cut(s) 5, 303, 536, 577, 599, 680, 1065
BspCNI CTCAG 2 cut(s) 40, 203
BspDI ATCGAT 1 cut(s) 684
BspFNI CGCG 3 cut(s) 241, 251, 902
BspLI GGNNCC 7 cut(s) 15, 36, 255, 372, 585, 743, 967
BspPI GGATC 1 cut(s) 996
BspT107I GGYRCC 3 cut(s) 370, 741, 965
BspTNI GGTCTC 1 cut(s) 764
BsrFI RCCGGY 3 cut(s) 278, 367, 738
BsrI ACTGG 2 cut(s) 20, 719
BssAI RCCGGY 3 cut(s) 278, 367, 738
BssECI CCNNGG 9 cut(s) 27, 75, 249, 391, 414, 415, 571, 834, 961
BssMI GATC 4 cut(s) 685, 892, 988, 1162
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 961
Bst2UI CCWGG 2 cut(s) 416, 836
Bst4CI ACNGT 5 cut(s) 88, 322, 583, 624, 890
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 310, 431
BstDEI CTNAG 5 cut(s) 48, 117, 122, 190, 260
BstDSI CCRYGG 2 cut(s) 249, 961
BstEII GGTNACC 1 cut(s) 904
BstF5I GGATG 5 cut(s) 67, 693, 707, 792, 1023
BstFNI CGCG 3 cut(s) 241, 251, 902
BstHHI GCGC 3 cut(s) 138, 243, 1158
BstKTI GATC 4 cut(s) 688, 895, 991, 1165
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 4 cut(s) 685, 892, 988, 1162
BstMCI CGRYCG 1 cut(s) 936
BstMWI GCNNNNNNNGC 3 cut(s) 221, 309, 542
BstNI CCWGG 2 cut(s) 416, 836
BstNSI RCATGY 2 cut(s) 312, 643
BstPAI GACNNNNGTC 1 cut(s) 776
BstPI GGTNACC 1 cut(s) 904
BstSCI CCNGG 6 cut(s) 26, 373, 414, 504, 834, 998
BstSLI GKGCMC 1 cut(s) 970
BstUI CGCG 3 cut(s) 241, 251, 902
BstV1I GCAGC 2 cut(s) 365, 511
BstX2I RGATCY 1 cut(s) 988
BstXI CCANNNNNNTGG 1 cut(s) 782
BstYI RGATCY 1 cut(s) 988
Bsu15I ATCGAT 1 cut(s) 684
BsuRI GGCC 7 cut(s) 5, 303, 536, 577, 599, 680, 1065
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 2 cut(s) 249, 961
BtgZI GCGATG 2 cut(s) 504, 674
BtsCI GGATG 5 cut(s) 67, 693, 707, 792, 1023
BtsI GCAGTG 1 cut(s) 601
BtsIMutI CAGTG 2 cut(s) 327, 601
Cac8I GCNNGC 2 cut(s) 310, 431
CaiI CAGNNNCTG 1 cut(s) 622
CfoI GCGC 3 cut(s) 138, 243, 1158
Cfr10I RCCGGY 3 cut(s) 278, 367, 738
Cfr13I GGNCC 6 cut(s) 34, 232, 575, 583, 598, 678
Cfr42I CCGCGG 1 cut(s) 252
ClaI ATCGAT 1 cut(s) 684
Csp6I GTAC 3 cut(s) 742, 1053, 1113
CspAI ACCGGT 3 cut(s) 278, 367, 738
CviAII CATG 5 cut(s) 309, 479, 640, 867, 962
CviQI GTAC 3 cut(s) 742, 1053, 1113
DdeI CTNAG 5 cut(s) 48, 117, 122, 190, 260
DpnI GATC 4 cut(s) 687, 894, 990, 1164
DpnII GATC 4 cut(s) 685, 892, 988, 1162
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 3 cut(s) 3, 534, 1063
Ecl136II GAGCTC 2 cut(s) 343, 766
Eco130I CCWWGG 1 cut(s) 961
Eco24I GRGCYC 2 cut(s) 345, 768
Eco31I GGTCTC 1 cut(s) 764
Eco47I GGWCC 3 cut(s) 34, 232, 583
Eco53kI GAGCTC 2 cut(s) 343, 766
Eco88I CYCGRG 2 cut(s) 572, 767
Eco91I GGTNACC 1 cut(s) 904
EcoICRI GAGCTC 2 cut(s) 343, 766
EcoO109I RGGNCCY 1 cut(s) 34
EcoO65I GGTNACC 1 cut(s) 904
EcoRI GAATTC 1 cut(s) 1073
EcoRII CCWGG 2 cut(s) 414, 834
EcoT14I CCWWGG 1 cut(s) 961
EcoT22I ATGCAT 1 cut(s) 1176
EcoT38I GRGCYC 2 cut(s) 345, 768
ErhI CCWWGG 1 cut(s) 961
FaeI CATG 5 cut(s) 312, 482, 643, 870, 965
FalI AAGNNNNNCTT 2 cut(s) 905, 937
FaqI GGGAC 1 cut(s) 569
FatI CATG 5 cut(s) 308, 478, 639, 866, 961
FauI CCCGC 1 cut(s) 246
FblI GTMKAC 2 cut(s) 81, 180
Fnu4HI GCNGC 4 cut(s) 187, 222, 379, 500
FokI GGATG 5 cut(s) 74, 680, 694, 799, 1030
FriOI GRGCYC 2 cut(s) 345, 768
Fsp4HI GCNGC 4 cut(s) 187, 222, 379, 500
FspBI CTAG 4 cut(s) 714, 813, 845, 1110
FspI TGCGCA 1 cut(s) 1157
GlaI GCGC 3 cut(s) 137, 242, 1157
GluI GCNGC 4 cut(s) 187, 222, 379, 500
HaeIII GGCC 7 cut(s) 5, 303, 536, 577, 599, 680, 1065
HapII CCGG 9 cut(s) 27, 279, 300, 368, 374, 506, 739, 932, 999
HhaI GCGC 3 cut(s) 138, 243, 1158
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 5 cut(s) 312, 482, 643, 870, 965
Hin6I GCGC 3 cut(s) 136, 241, 1156
HinP1I GCGC 3 cut(s) 136, 241, 1156
HincII GTYRAC 3 cut(s) 82, 181, 627
HindII GTYRAC 3 cut(s) 82, 181, 627
HindIII AAGCTT 2 cut(s) 119, 1122
HinfI GANTC 2 cut(s) 50, 928
HpaII CCGG 9 cut(s) 27, 279, 300, 368, 374, 506, 739, 932, 999
HphI GGTGA 3 cut(s) 100, 898, 998
Hpy166II GTNNAC 6 cut(s) 82, 181, 267, 627, 940, 1055
Hpy188I TCNGA 4 cut(s) 58, 285, 1017, 1225
Hpy188III TCNNGA 4 cut(s) 11, 590, 769, 925
Hpy8I GTNNAC 6 cut(s) 82, 181, 267, 627, 940, 1055
Hpy99I CGWCG 3 cut(s) 92, 173, 221
HpyAV CCTTC 3 cut(s) 306, 395, 850
HpyCH4III ACNGT 5 cut(s) 88, 322, 583, 624, 890
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 312, 1174, 1205
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 309, 542
HpyF3I CTNAG 5 cut(s) 48, 117, 122, 190, 260
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 5 cut(s) 312, 482, 643, 870, 965
HspAI GCGC 3 cut(s) 136, 241, 1156
KpnI GGTACC 1 cut(s) 745
KspI CCGCGG 1 cut(s) 252
Kzo9I GATC 4 cut(s) 685, 892, 988, 1162
LmnI GCTCC 3 cut(s) 507, 550, 1111
Lsp1109I GCAGC 2 cut(s) 365, 511
LweI GCATC 4 cut(s) 123, 501, 702, 777
MaeI CTAG 4 cut(s) 714, 813, 845, 1110
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 316, 904
MalI GATC 4 cut(s) 687, 894, 990, 1164
MboI GATC 4 cut(s) 685, 892, 988, 1162
MboII GAAGA 2 cut(s) 822, 865
MflI RGATCY 1 cut(s) 988
MhlI GDGCHC 4 cut(s) 345, 400, 768, 970
MlsI TGGCCA 2 cut(s) 5, 536
MluCI AATT 4 cut(s) 199, 983, 1011, 1073
MluNI TGGCCA 2 cut(s) 5, 536
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 387
Mox20I TGGCCA 2 cut(s) 5, 536
Mph1103I ATGCAT 1 cut(s) 1176
MscI TGGCCA 2 cut(s) 5, 536
MseI TTAA 4 cut(s) 153, 198, 723, 1127
Msp20I TGGCCA 2 cut(s) 5, 536
MspA1I CMGCKG 2 cut(s) 194, 251
MspI CCGG 9 cut(s) 27, 279, 300, 368, 374, 506, 739, 932, 999
MspR9I CCNGG 6 cut(s) 28, 375, 416, 506, 836, 1000
Mva1269I GAATGC 2 cut(s) 1046, 1176
MvaI CCWGG 2 cut(s) 416, 836
MvnI CGCG 3 cut(s) 241, 251, 902
MwoI GCNNNNNNNGC 3 cut(s) 221, 309, 542
NciI CCSGG 4 cut(s) 28, 375, 506, 1000
NcoI CCATGG 1 cut(s) 961
NdeII GATC 4 cut(s) 685, 892, 988, 1162
NlaIII CATG 5 cut(s) 312, 482, 643, 870, 965
NlaIV GGNNCC 7 cut(s) 15, 36, 255, 372, 585, 743, 967
NmuCI GTSAC 1 cut(s) 904
NsbI TGCGCA 1 cut(s) 1157
NsiI ATGCAT 1 cut(s) 1176
NspI RCATGY 2 cut(s) 312, 643
PaeI GCATGC 1 cut(s) 312
PaeR7I CTCGAG 1 cut(s) 767
PasI CCCWGGG 1 cut(s) 415
PciI ACATGT 1 cut(s) 639
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 177
PctI GAATGC 2 cut(s) 1046, 1176
PfeI GAWTC 1 cut(s) 928
PflFI GACNNNGTC 1 cut(s) 86
PinAI ACCGGT 3 cut(s) 278, 367, 738
PkrI GCNGC 4 cut(s) 188, 223, 380, 501
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PpuMI RGGWCCY 1 cut(s) 34
PscI ACATGT 1 cut(s) 639
PshAI GACNNNNGTC 1 cut(s) 776
PshBI ATTAAT 1 cut(s) 153
Psp124BI GAGCTC 2 cut(s) 345, 768
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 2 cut(s) 414, 834
PspEI GGTNACC 1 cut(s) 904
PspGI CCWGG 2 cut(s) 414, 834
PspN4I GGNNCC 7 cut(s) 15, 36, 255, 372, 585, 743, 967
PspPI GGNCC 6 cut(s) 34, 232, 575, 583, 598, 678
PspPPI RGGWCCY 1 cut(s) 34
PstNI CAGNNNCTG 1 cut(s) 622
PsuI RGATCY 1 cut(s) 988
PsyI GACNNNGTC 1 cut(s) 86
RsaI GTAC 3 cut(s) 743, 1054, 1114
RsaNI GTAC 3 cut(s) 742, 1053, 1113
SacI GAGCTC 2 cut(s) 345, 768
SacII CCGCGG 1 cut(s) 252
SalI GTCGAC 2 cut(s) 80, 179
SaqAI TTAA 4 cut(s) 153, 198, 723, 1127
SatI GCNGC 4 cut(s) 187, 222, 379, 500
Sau3AI GATC 4 cut(s) 685, 892, 988, 1162
Sau96I GGNCC 6 cut(s) 34, 232, 575, 583, 598, 678
ScaI AGTACT 1 cut(s) 1114
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 6 cut(s) 28, 375, 416, 506, 836, 1000
SduI GDGCHC 4 cut(s) 345, 400, 768, 970
SfaNI GCATC 4 cut(s) 123, 501, 702, 777
Sfr274I CTCGAG 1 cut(s) 767
Sfr303I CCGCGG 1 cut(s) 252
SgrBI CCGCGG 1 cut(s) 252
SinI GGWCC 3 cut(s) 34, 232, 583
SlaI CTCGAG 1 cut(s) 767
SmlI CTYRAG 2 cut(s) 588, 767
SmoI CTYRAG 2 cut(s) 588, 767
SpeI ACTAGT 1 cut(s) 844
SphI GCATGC 1 cut(s) 312
Sse9I AATT 4 cut(s) 199, 983, 1011, 1073
SsiI CCGC 9 cut(s) 186, 194, 222, 239, 249, 251, 711, 902, 1148
SspMI CTAG 4 cut(s) 714, 813, 845, 1110
SstI GAGCTC 2 cut(s) 345, 768
StyD4I CCNGG 6 cut(s) 26, 373, 414, 504, 834, 998
StyI CCWWGG 1 cut(s) 961
TaaI ACNGT 5 cut(s) 88, 322, 583, 624, 890
TaiI ACGT 1 cut(s) 174
TaqI TCGA 6 cut(s) 81, 149, 168, 180, 684, 768
TasI AATT 4 cut(s) 199, 983, 1011, 1073
TatI WGTACW 1 cut(s) 1112
TauI GCSGC 2 cut(s) 189, 224
TfiI GAWTC 1 cut(s) 928
Tru1I TTAA 4 cut(s) 153, 198, 723, 1127
Tru9I TTAA 4 cut(s) 153, 198, 723, 1127
TscAI CASTG 2 cut(s) 327, 608
TseFI GTSAC 1 cut(s) 904
TseI GCWGC 2 cut(s) 378, 499
Tsp45I GTSAC 1 cut(s) 904
TspDTI ATGAA 5 cut(s) 78, 412, 969, 1086, 1145
TspGWI ACGGA 2 cut(s) 983, 1040
TspRI CASTG 2 cut(s) 327, 608
Tth111I GACNNNGTC 1 cut(s) 86
VpaK11BI GGWCC 3 cut(s) 34, 232, 583
VspI ATTAAT 1 cut(s) 153
XapI RAATTY 2 cut(s) 1011, 1073
XceI RCATGY 2 cut(s) 312, 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 767
XmiI GTMKAC 2 cut(s) 81, 180
XspI CTAG 4 cut(s) 714, 813, 845, 1110
ZraI GACGTC 1 cut(s) 172
ZrmI AGTACT 1 cut(s) 1114
Zsp2I ATGCAT 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.