FvH4_4g14286
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001157
Physical Location & Seq
Forward (+)
17877607 .. 17878401
795 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14286.t1

Sequence Viewer

Length: 795 bp
ATGGGTGACGCACCGATTACTAGAGCAGACCTAGAAGGTATCCTAGAAGGTGTTACTGCGAATTTTACCGCAGCCCTTGCCACGTTGACTAATCAGGTGAACGAATTGAAGACTGATAATAACAATAGGAAGCGTGGAGAAGGCAAAGCTATTCCTCAACATAGGGTTCCACGAGGGAGAGGAACCACAATTGCTAGCAGCTCTGAGTCTGATTCAGAAGATGAAGTTGTTGATCTGGCGCAATTAGGGCAAGATGATAAGGATTTCAGGATGAAGGTTGATATCCCTTTCTTTAATGGGATGATGAACGTGGAGGAATTTCTGGATTGGCAAATTGAAGTGGACAGATTCATAGAGCATATGAATGTCGCGGAACATAAGCAAGTGAAGTTGGTGGTAGCTCATCTCAAGAGCACTGCGGCAGTTTGGTGGGATAAGTTGGCATTGACGAGATTGAGACAGAGAAAAAAACCCGTCAAGAATTGGCGGAGGATGAAACAGTTGATGACAGAACGATTCCTGCCAGAGGACTATGAACAGATTCTTTACAGGATGTACCTAGATTGTGTTCAAGGGGCGAAGACTGTAACAGAGTATACCGCTGAGTTTGTGCGTCTTTCTGAGCGTAATGACTTAGGCGAATCTGAAGGGCAGAAGGTTGCTTGGTACATCAGTGGGTTGAGACCTTCCATACAAGAGAAGATTGGCTTACAAACTATGTGGTCTGTGACTGAGGCTGCAAGTTTGGCGATAAAGGCGGAGCTGATGGAGAGATCCCCTAGAGTTTCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-H2C2

Protein Analysis

265

Amino Acids

30.25

Weight (kDa)

5.93

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 99 - 254 3.2e-07 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 130 - 229 6.6e-14 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 596
AccII CGCG 1 cut(s) 371
AciI CCGC 6 cut(s) 69, 371, 419, 487, 600, 758
AclWI GGATC 1 cut(s) 768
AcsI RAATTY 2 cut(s) 61, 317
AcuI CTGAAG 1 cut(s) 666
AfaI GTAC 2 cut(s) 557, 668
AfiI CCNNNNNNNGG 1 cut(s) 526
AgsI TTSAA 3 cut(s) 109, 338, 572
AluBI AGCT 4 cut(s) 149, 201, 401, 763
AluI AGCT 4 cut(s) 149, 201, 401, 763
Alw21I GWGCWC 1 cut(s) 416
Alw26I GTCTC 2 cut(s) 451, 676
AlwI GGATC 1 cut(s) 768
ApeKI GCWGC 3 cut(s) 71, 198, 737
ApoI RAATTY 2 cut(s) 61, 317
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 1 cut(s) 241
AsuHPI GGTGA 2 cut(s) 17, 109
AsuNHI GCTAGC 1 cut(s) 194
BaeI ACNNNNGTAYC 2 cut(s) 658, 691
BauI CACGAG 1 cut(s) 171
BbsI GAAGAC 2 cut(s) 116, 587
Bbv12I GWGCWC 1 cut(s) 416
BbvI GCAGC 3 cut(s) 83, 210, 724
BccI CCATC 1 cut(s) 760
BciVI GTATCC 1 cut(s) 50
BcoDI GTCTC 2 cut(s) 451, 676
BfaI CTAG 6 cut(s) 21, 32, 44, 195, 560, 780
BfuI GTATCC 1 cut(s) 50
BisI GCNGC 4 cut(s) 72, 199, 420, 738
BlsI GCNGC 4 cut(s) 73, 200, 421, 739
BmiI GGNNCC 2 cut(s) 168, 184
BmtI GCTAGC 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 116, 587
BpuEI CTTGAG 1 cut(s) 392
BsaI GGTCTC 1 cut(s) 676
Bsc4I CCNNNNNNNGG 1 cut(s) 526
BseGI GGATG 4 cut(s) 276, 306, 498, 558
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMII CTCAG 4 cut(s) 195, 594, 612, 723
BseXI GCAGC 3 cut(s) 83, 210, 724
Bsh1236I CGCG 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 416
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 2 cut(s) 451, 676
Bso31I GGTCTC 1 cut(s) 676
Bsp1286I GDGCHC 1 cut(s) 416
Bsp143I GATC 2 cut(s) 232, 773
BspACI CCGC 6 cut(s) 69, 371, 419, 487, 600, 758
BspCNI CTCAG 4 cut(s) 196, 595, 613, 724
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 2 cut(s) 168, 184
BspOI GCTAGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 768
BspTNI GGTCTC 1 cut(s) 676
BssMI GATC 2 cut(s) 232, 773
BssNAI GTATAC 1 cut(s) 597
BssSI CACGAG 1 cut(s) 171
Bst1107I GTATAC 1 cut(s) 597
Bst2BI CACGAG 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 501, 586
BstAPI GCANNNNNTGC 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 5 cut(s) 204, 603, 621, 634, 732
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 4 cut(s) 276, 306, 498, 558
BstFNI CGCG 1 cut(s) 371
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 2 cut(s) 235, 776
BstMAI GTCTC 2 cut(s) 451, 676
BstMBI GATC 2 cut(s) 232, 773
BstMWI GCNNNNNNNGC 4 cut(s) 77, 247, 746, 755
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 3 cut(s) 83, 210, 724
BstV2I GAAGAC 2 cut(s) 116, 587
BstX2I RGATCY 1 cut(s) 773
BstYI RGATCY 1 cut(s) 773
BstZ17I GTATAC 1 cut(s) 597
BsuI GTATCC 1 cut(s) 50
BtsCI GGATG 4 cut(s) 276, 306, 498, 558
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 2 cut(s) 414, 679
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 1 cut(s) 241
CseI GACGC 2 cut(s) 17, 602
Csp6I GTAC 2 cut(s) 556, 667
CspCI CAANNNNNGTGG 2 cut(s) 701, 736
CviJI RGCY 7 cut(s) 74, 149, 201, 401, 708, 737, 763
CviKI_1 RGCY 7 cut(s) 74, 149, 201, 401, 708, 737, 763
CviQI GTAC 2 cut(s) 556, 667
DdeI CTNAG 5 cut(s) 204, 603, 621, 634, 732
DpnI GATC 2 cut(s) 234, 775
DpnII GATC 2 cut(s) 232, 773
EciI GGCGGA 2 cut(s) 502, 773
Eco31I GGTCTC 1 cut(s) 676
Eco32I GATATC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 666
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoRV GATATC 1 cut(s) 283
FaiI YATR 9 cut(s) 162, 353, 360, 362, 378, 534, 597, 692, 719
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FauNDI CATATG 1 cut(s) 360
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 4 cut(s) 72, 199, 420, 738
FokI GGATG 4 cut(s) 283, 313, 505, 565
Fsp4HI GCNGC 4 cut(s) 72, 199, 420, 738
FspBI CTAG 6 cut(s) 21, 32, 44, 195, 560, 780
GlaI GCGC 1 cut(s) 240
GluI GCNGC 4 cut(s) 72, 199, 420, 738
HgaI GACGC 2 cut(s) 17, 602
HhaI GCGC 1 cut(s) 241
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 6 cut(s) 206, 212, 348, 516, 541, 641
HphI GGTGA 2 cut(s) 17, 109
Hpy166II GTNNAC 4 cut(s) 87, 100, 343, 597
Hpy188I TCNGA 5 cut(s) 205, 211, 217, 622, 646
Hpy188III TCNNGA 4 cut(s) 268, 323, 409, 478
Hpy8I GTNNAC 4 cut(s) 87, 100, 343, 597
HpyAV CCTTC 7 cut(s) 29, 41, 134, 268, 641, 649, 696
HpyCH4III ACNGT 2 cut(s) 501, 586
HpyCH4IV ACGT 2 cut(s) 83, 309
HpyCH4V TGCA 1 cut(s) 740
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 247, 746, 755
HpyF3I CTNAG 5 cut(s) 204, 603, 621, 634, 732
HpySE526I ACGT 2 cut(s) 83, 309
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 2 cut(s) 232, 773
LmnI GCTCC 1 cut(s) 760
LpnPI CCDG 7 cut(s) 80, 221, 253, 308, 533, 535, 537
Lsp1109I GCAGC 3 cut(s) 83, 210, 724
MaeI CTAG 6 cut(s) 21, 32, 44, 195, 560, 780
MaeII ACGT 2 cut(s) 83, 309
MaeIII GTNAC 4 cut(s) 5, 52, 586, 727
MalI GATC 2 cut(s) 234, 775
MboI GATC 2 cut(s) 232, 773
MboII GAAGA 4 cut(s) 121, 230, 592, 712
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 1 cut(s) 416
MluCI AATT 7 cut(s) 61, 104, 189, 242, 317, 333, 481
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 7 cut(s) 165, 167, 173, 307, 483, 520, 727
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 602
MunI CAATTG 1 cut(s) 189
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 4 cut(s) 77, 247, 746, 755
NdeI CATATG 1 cut(s) 360
NdeII GATC 2 cut(s) 232, 773
NheI GCTAGC 1 cut(s) 194
NlaIV GGNNCC 2 cut(s) 168, 184
NmuCI GTSAC 2 cut(s) 5, 727
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 5 cut(s) 212, 348, 516, 541, 641
PkrI GCNGC 4 cut(s) 73, 200, 421, 739
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 168, 184
PsuI RGATCY 1 cut(s) 773
RsaI GTAC 2 cut(s) 557, 668
RsaNI GTAC 2 cut(s) 556, 667
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 4 cut(s) 72, 199, 420, 738
Sau3AI GATC 2 cut(s) 232, 773
SchI GAGTC 1 cut(s) 215
SduI GDGCHC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 7 cut(s) 61, 104, 189, 242, 317, 333, 481
SsiI CCGC 6 cut(s) 69, 371, 419, 487, 600, 758
SspMI CTAG 6 cut(s) 21, 32, 44, 195, 560, 780
TaaI ACNGT 2 cut(s) 501, 586
TaiI ACGT 2 cut(s) 86, 312
TasI AATT 7 cut(s) 61, 104, 189, 242, 317, 333, 481
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 5 cut(s) 212, 348, 516, 541, 641
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 421, 679
TseFI GTSAC 2 cut(s) 5, 727
TseI GCWGC 3 cut(s) 71, 198, 737
Tsp45I GTSAC 2 cut(s) 5, 727
TspDTI ATGAA 7 cut(s) 237, 287, 320, 340, 377, 509, 549
TspRI CASTG 2 cut(s) 421, 679
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 2 cut(s) 61, 317
XmiI GTMKAC 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 6 cut(s) 21, 32, 44, 195, 560, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.