Rroxscaffold_5G00359430

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001157
Physical Location & Seq
Reverse (-)
39633655 .. 39636496
2842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00359430.1

Sequence Viewer

Length: 1146 bp
ATGTGGATGAACACCTTCGAACAACATTTTCCTGCTGCCACGTGGGATCAGTTCACCTCCGTATTTCTGGAACACCATGGTGCCAACAACACCGTGGACTTCAAAATTGCTCTCTCCCAATTAAAGCAGTCCTCCTCGGTGGATGAATTCATAGTGGCTTTCACGAAACTCGCCGGACGAGTTAGAGGATGGTCGGATTCCGAGTTGCTCCCTCTCTTCCTGAGTGGGTTGAAACCAGAAATCCGGCACAACGTGATCGCCTTAGAGCCCAACACTCTGGTCTCGGCTCAGCCCCTGGCACGCCGCTTTGAGATCAAGTTGGCGGACATCCGCTCAGCCCGACAACACCGTACCTCCTCTTGGCATTACTCTCCCAAACTCACCCAATACCCCCACAACCCCTCTGCACCCACTACCAGCCAATCCATTGTGCCCCACAATCCAAATCCCAACAATTTCACAAACAACCACCAAACCACCCCTAACCAGAGAACAAAGACTGAGGGCCCATACTGTAAGTGGTCCGTGGCCGAACAAAGAGAACGCAAAGCACAAGGGCTCTGTTACAGCTGTGACGAGCAATGGTCTAAGACTCATGTTTGCAAGAAGCCAATCTTAGCCATTTTGGAGGTACCTGGACCAACTGAGGAGCCATGTATTGAAGAGCTCCAAGAACATGATCCGGGTTCTGATGCCGACCAAGCTCTGCTACTGCACGCCATCACCACCACGACCATAGGTGAAATGATGCGCTTTGCGAGCTCTATTAATGGGCACCTAATTGAGATTTTCATCAATTGTGGATCTGCTACAAACTTCTTGAACCCGGTGGTTGCTCGCAATTTGGGCCTATCCATCACCGAAGGCTCGCCCTTGCGCTTCACCACAGCCTCCGGTCATTCTGTCTCCTCGTCTGGGATCGCCACTGACTTGCCTGTCTCCATCCAAAGCTTCAGCTTCGTAAGCTCTTTCCTTCTCATGCCGGTAACGGGCTGTGACCTCCTCTTCGAAGCCCAATGGCTGGACACCTTGAGATTCATTGGGTGGCATTTTCTAGAGAAGGTTATGATGCTCCAGGTCAATGGAGTTTACCATGTATTACAAAGGATTACGAAAGTACTCCTCAGTTGGATCAGGGTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

381

Amino Acids

42.76

Weight (kDa)

6.59

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 2 - 79 1.4e-09 Retrotransposon gag protein
Ty3_capsid PF19259 2 - 101 3.8e-09 Ty3 transposon capsid-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 935
Acc65I GGTACC 1 cut(s) 631
AccB1I GGYRCC 4 cut(s) 80, 631, 774, 1138
AccB7I CCANNNNNTGG 1 cut(s) 1021
AccBSI CCGCTC 1 cut(s) 333
AciI CCGC 3 cut(s) 304, 323, 331
AclWI GGATC 5 cut(s) 54, 674, 811, 926, 1139
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 1 cut(s) 937
AcvI CACGTG 1 cut(s) 42
AdeI CACNNNGTG 1 cut(s) 253
AfaI GTAC 3 cut(s) 352, 633, 1119
AfiI CCNNNNNNNGG 4 cut(s) 360, 915, 989, 1021
AgsI TTSAA 4 cut(s) 103, 232, 662, 823
AjnI CCWGG 3 cut(s) 294, 634, 1074
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 7 cut(s) 570, 667, 704, 762, 951, 957, 966
AluI AGCT 7 cut(s) 570, 667, 704, 762, 951, 957, 966
Alw21I GWGCWC 2 cut(s) 669, 764
Alw26I GTCTC 3 cut(s) 286, 910, 943
AlwI GGATC 5 cut(s) 54, 674, 811, 926, 1139
AlwNI CAGNNNCTG 1 cut(s) 295
AoxI GGCC 3 cut(s) 505, 528, 847
ApaI GGGCCC 1 cut(s) 509
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 1 cut(s) 146
ArsI GACNNNNNNTTYG 2 cut(s) 989, 1021
AseI ATTAAT 1 cut(s) 768
Asp700I GAANNNNTTC 1 cut(s) 14
Asp718I GGTACC 1 cut(s) 631
AspLEI GCGC 2 cut(s) 753, 879
AspS9I GGNCC 5 cut(s) 505, 506, 522, 638, 847
AsuC2I CCSGG 2 cut(s) 684, 827
AsuHPI GGTGA 6 cut(s) 46, 373, 715, 752, 850, 874
AsuII TTCGAA 2 cut(s) 18, 1008
AvaII GGWCC 2 cut(s) 522, 638
BaeGI GKGCMC 3 cut(s) 435, 509, 777
BanI GGYRCC 4 cut(s) 80, 631, 774, 1138
BanII GRGCYC 5 cut(s) 270, 509, 561, 669, 764
BbrPI CACGTG 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 669, 764
BbvI GCAGC 1 cut(s) 22
BccI CCATC 4 cut(s) 183, 728, 863, 950
BciT130I CCWGG 3 cut(s) 296, 636, 1076
BcnI CCSGG 2 cut(s) 684, 827
BcoDI GTCTC 3 cut(s) 286, 910, 943
BfaI CTAG 1 cut(s) 1055
BisI GCNGC 2 cut(s) 36, 304
BlpI GCTNAGC 2 cut(s) 288, 334
BlsI GCNGC 2 cut(s) 37, 305
BmcAI AGTACT 1 cut(s) 1119
Bme1390I CCNGG 5 cut(s) 296, 636, 684, 827, 1076
Bme18I GGWCC 2 cut(s) 522, 638
BmgT120I GGNCC 5 cut(s) 505, 506, 522, 638, 847
BmiI GGNNCC 6 cut(s) 82, 507, 633, 651, 776, 1140
BmrFI CCNGG 5 cut(s) 296, 636, 684, 827, 1076
BmsI GCATC 3 cut(s) 682, 738, 1059
BpmI CTGGAG 1 cut(s) 1058
Bpu1102I GCTNAGC 2 cut(s) 288, 334
Bpu14I TTCGAA 2 cut(s) 18, 1008
BpuEI CTTGAG 1 cut(s) 1051
BpuMI CCSGG 2 cut(s) 684, 827
BsaAI YACGTR 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 791
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 5 cut(s) 76, 93, 135, 294, 525
BsaWI WCCGGW 1 cut(s) 893
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 360, 915, 989, 1021
Bse118I RCCGGY 1 cut(s) 982
Bse3DI GCAATG 1 cut(s) 587
Bse8I GATNNNNATC 1 cut(s) 791
BseBI CCWGG 3 cut(s) 296, 636, 1076
BseDI CCNNGG 5 cut(s) 76, 93, 135, 294, 525
BseGI GGATG 5 cut(s) 12, 148, 194, 327, 942
BseJI GATNNNNATC 1 cut(s) 791
BseLI CCNNNNNNNGG 4 cut(s) 360, 915, 989, 1021
BseMI GCAATG 1 cut(s) 587
BseMII CTCAG 6 cut(s) 212, 302, 348, 492, 636, 1138
BseRI GAGGAG 6 cut(s) 124, 346, 662, 898, 992, 1112
BseSI GKGCMC 3 cut(s) 435, 509, 777
BseXI GCAGC 1 cut(s) 22
BsgI GTGCAG 2 cut(s) 390, 698
BshFI GGCC 3 cut(s) 507, 530, 849
BshNI GGYRCC 4 cut(s) 80, 631, 774, 1138
BsiHKAI GWGCWC 2 cut(s) 669, 764
BsiSI CCGG 6 cut(s) 174, 244, 683, 827, 894, 983
BslI CCNNNNNNNGG 4 cut(s) 360, 915, 989, 1021
BsmAI GTCTC 3 cut(s) 286, 910, 943
BsnI GGCC 3 cut(s) 507, 530, 849
Bso31I GGTCTC 1 cut(s) 286
Bsp119I TTCGAA 2 cut(s) 18, 1008
Bsp120I GGGCCC 1 cut(s) 505
Bsp1286I GDGCHC 7 cut(s) 270, 435, 509, 561, 669, 764, 777
Bsp143I GATC 7 cut(s) 46, 255, 312, 679, 803, 918, 1131
Bsp1720I GCTNAGC 2 cut(s) 288, 334
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 3 cut(s) 304, 323, 331
BspANI GGCC 3 cut(s) 507, 530, 849
BspCNI CTCAG 6 cut(s) 213, 301, 347, 493, 637, 1137
BspLI GGNNCC 6 cut(s) 82, 507, 633, 651, 776, 1140
BspPI GGATC 5 cut(s) 54, 674, 811, 926, 1139
BspQI GCTCTTC 1 cut(s) 657
BspT104I TTCGAA 2 cut(s) 18, 1008
BspT107I GGYRCC 4 cut(s) 80, 631, 774, 1138
BspTNI GGTCTC 1 cut(s) 286
BsrBI CCGCTC 1 cut(s) 333
BsrDI GCAATG 1 cut(s) 587
BsrFI RCCGGY 1 cut(s) 982
BssAI RCCGGY 1 cut(s) 982
BssECI CCNNGG 5 cut(s) 76, 93, 135, 294, 525
BssMI GATC 7 cut(s) 46, 255, 312, 679, 803, 918, 1131
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 3 cut(s) 296, 636, 1076
Bst4CI ACNGT 3 cut(s) 94, 350, 515
Bst6I CTCTTC 3 cut(s) 221, 657, 1010
BstBAI YACGTR 1 cut(s) 42
BstBI TTCGAA 2 cut(s) 18, 1008
BstC8I GCNNGC 5 cut(s) 301, 717, 760, 838, 869
BstDEI CTNAG 9 cut(s) 221, 262, 288, 334, 501, 588, 616, 645, 1124
BstDSI CCRYGG 3 cut(s) 76, 93, 525
BstF5I GGATG 5 cut(s) 12, 148, 194, 327, 942
BstHHI GCGC 2 cut(s) 753, 879
BstKTI GATC 7 cut(s) 49, 258, 315, 682, 806, 921, 1134
BstMAI GTCTC 3 cut(s) 286, 910, 943
BstMBI GATC 7 cut(s) 46, 255, 312, 679, 803, 918, 1131
BstMWI GCNNNNNNNGC 4 cut(s) 701, 759, 846, 963
BstNI CCWGG 3 cut(s) 296, 636, 1076
BstSCI CCNGG 5 cut(s) 294, 634, 682, 825, 1074
BstSLI GKGCMC 3 cut(s) 435, 509, 777
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 803
BstXI CCANNNNNNTGG 2 cut(s) 277, 1082
BstYI RGATCY 1 cut(s) 803
BsuRI GGCC 3 cut(s) 507, 530, 849
BtgI CCRYGG 3 cut(s) 76, 93, 525
BtsCI GGATG 5 cut(s) 12, 148, 194, 327, 942
BtsIMutI CAGTG 1 cut(s) 924
Cac8I GCNNGC 5 cut(s) 301, 717, 760, 838, 869
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 2 cut(s) 753, 879
Cfr10I RCCGGY 1 cut(s) 982
Cfr13I GGNCC 5 cut(s) 505, 506, 522, 638, 847
Csp6I GTAC 3 cut(s) 351, 632, 1118
CviAII CATG 6 cut(s) 77, 596, 654, 677, 979, 1094
CviQI GTAC 3 cut(s) 351, 632, 1118
DdeI CTNAG 9 cut(s) 221, 262, 288, 334, 501, 588, 616, 645, 1124
DpnI GATC 7 cut(s) 48, 257, 314, 681, 805, 920, 1133
DpnII GATC 7 cut(s) 46, 255, 312, 679, 803, 918, 1131
DraIII CACNNNGTG 1 cut(s) 253
DrdI GACNNNNNNGTC 1 cut(s) 935
DseDI GACNNNNNNGTC 1 cut(s) 935
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 3 cut(s) 221, 657, 1010
EarI CTCTTC 3 cut(s) 221, 657, 1010
EciI GGCGGA 1 cut(s) 338
Ecl136II GAGCTC 2 cut(s) 667, 762
Eco130I CCWWGG 1 cut(s) 76
Eco24I GRGCYC 5 cut(s) 270, 509, 561, 669, 764
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 2 cut(s) 522, 638
Eco53kI GAGCTC 2 cut(s) 667, 762
Eco57I CTGAAG 1 cut(s) 937
Eco72I CACGTG 1 cut(s) 42
EcoICRI GAGCTC 2 cut(s) 667, 762
EcoO109I RGGNCCY 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 146
EcoRII CCWGG 3 cut(s) 294, 634, 1074
EcoT14I CCWWGG 1 cut(s) 76
EcoT38I GRGCYC 5 cut(s) 270, 509, 561, 669, 764
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 6 cut(s) 80, 599, 657, 680, 982, 1097
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FatI CATG 6 cut(s) 76, 595, 653, 676, 978, 1093
Fnu4HI GCNGC 2 cut(s) 36, 304
FokI GGATG 5 cut(s) 19, 155, 201, 314, 929
FriOI GRGCYC 5 cut(s) 270, 509, 561, 669, 764
Fsp4HI GCNGC 2 cut(s) 36, 304
FspBI CTAG 1 cut(s) 1055
GlaI GCGC 2 cut(s) 752, 878
GluI GCNGC 2 cut(s) 36, 304
GsuI CTGGAG 1 cut(s) 1058
HaeIII GGCC 3 cut(s) 507, 530, 849
HapII CCGG 6 cut(s) 174, 244, 683, 827, 894, 983
HhaI GCGC 2 cut(s) 753, 879
Hin1II CATG 6 cut(s) 80, 599, 657, 680, 982, 1097
Hin6I GCGC 2 cut(s) 751, 877
HinP1I GCGC 2 cut(s) 751, 877
HindIII AAGCTT 1 cut(s) 949
HinfI GANTC 3 cut(s) 197, 592, 1035
HpaII CCGG 6 cut(s) 174, 244, 683, 827, 894, 983
HphI GGTGA 6 cut(s) 46, 373, 715, 752, 850, 874
Hpy166II GTNNAC 3 cut(s) 54, 97, 1090
Hpy188I TCNGA 3 cut(s) 196, 202, 691
Hpy188III TCNNGA 5 cut(s) 68, 163, 220, 820, 1055
Hpy8I GTNNAC 3 cut(s) 54, 97, 1090
HpyAV CCTTC 4 cut(s) 25, 857, 983, 1054
HpyCH4III ACNGT 3 cut(s) 94, 350, 515
HpyCH4IV ACGT 2 cut(s) 41, 252
HpyCH4V TGCA 3 cut(s) 407, 603, 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 701, 759, 846, 963
HpyF3I CTNAG 9 cut(s) 221, 262, 288, 334, 501, 588, 616, 645, 1124
HpySE526I ACGT 2 cut(s) 41, 252
Hsp92II CATG 6 cut(s) 80, 599, 657, 680, 982, 1097
HspAI GCGC 2 cut(s) 751, 877
KpnI GGTACC 1 cut(s) 635
Kzo9I GATC 7 cut(s) 46, 255, 312, 679, 803, 918, 1131
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 4 cut(s) 213, 649, 672, 1077
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 3 cut(s) 682, 738, 1059
MaeI CTAG 1 cut(s) 1055
MaeII ACGT 2 cut(s) 41, 252
MaeIII GTNAC 4 cut(s) 563, 572, 985, 995
MalI GATC 7 cut(s) 48, 257, 314, 681, 805, 920, 1133
MbiI CCGCTC 1 cut(s) 333
MboI GATC 7 cut(s) 46, 255, 312, 679, 803, 918, 1131
MboII GAAGA 3 cut(s) 208, 674, 997
MfeI CAATTG 1 cut(s) 796
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 7 cut(s) 270, 435, 509, 561, 669, 764, 777
MluCI AATT 7 cut(s) 105, 119, 146, 454, 780, 796, 841
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 2 cut(s) 174, 1109
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 2 cut(s) 122, 768
MslI CAYNNNNRTG 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 570
MspI CCGG 6 cut(s) 174, 244, 683, 827, 894, 983
MspR9I CCNGG 5 cut(s) 296, 636, 684, 827, 1076
MunI CAATTG 1 cut(s) 796
MvaI CCWGG 3 cut(s) 296, 636, 1076
MwoI GCNNNNNNNGC 4 cut(s) 701, 759, 846, 963
NciI CCSGG 2 cut(s) 684, 827
NcoI CCATGG 1 cut(s) 76
NdeII GATC 7 cut(s) 46, 255, 312, 679, 803, 918, 1131
NlaIII CATG 6 cut(s) 80, 599, 657, 680, 982, 1097
NlaIV GGNNCC 6 cut(s) 82, 507, 633, 651, 776, 1140
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 2 cut(s) 572, 995
NspV TTCGAA 2 cut(s) 18, 1008
OliI CACNNNNGTG 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 657
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 2 cut(s) 197, 1035
PflMI CCANNNNNTGG 1 cut(s) 1021
PkrI GCNGC 2 cut(s) 37, 305
PleI GAGTC 1 cut(s) 586
PmaCI CACGTG 1 cut(s) 42
PmlI CACGTG 1 cut(s) 42
PpsI GAGTC 1 cut(s) 586
Ppu21I YACGTR 1 cut(s) 42
PshBI ATTAAT 1 cut(s) 768
Psp124BI GAGCTC 2 cut(s) 669, 764
Psp6I CCWGG 3 cut(s) 294, 634, 1074
PspCI CACGTG 1 cut(s) 42
PspGI CCWGG 3 cut(s) 294, 634, 1074
PspN4I GGNNCC 6 cut(s) 82, 507, 633, 651, 776, 1140
PspOMI GGGCCC 1 cut(s) 505
PspPI GGNCC 5 cut(s) 505, 506, 522, 638, 847
PstNI CAGNNNCTG 1 cut(s) 295
PsuI RGATCY 1 cut(s) 803
PvuII CAGCTG 1 cut(s) 570
RsaI GTAC 3 cut(s) 352, 633, 1119
RsaNI GTAC 3 cut(s) 351, 632, 1118
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 2 cut(s) 669, 764
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 2 cut(s) 122, 768
SatI GCNGC 2 cut(s) 36, 304
Sau3AI GATC 7 cut(s) 46, 255, 312, 679, 803, 918, 1131
Sau96I GGNCC 5 cut(s) 505, 506, 522, 638, 847
ScaI AGTACT 1 cut(s) 1119
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 5 cut(s) 296, 636, 684, 827, 1076
SduI GDGCHC 7 cut(s) 270, 435, 509, 561, 669, 764, 777
SfaNI GCATC 3 cut(s) 682, 738, 1059
SfuI TTCGAA 2 cut(s) 18, 1008
SinI GGWCC 2 cut(s) 522, 638
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 1030
SmoI CTYRAG 1 cut(s) 1030
Sse9I AATT 7 cut(s) 105, 119, 146, 454, 780, 796, 841
SsiI CCGC 3 cut(s) 304, 323, 331
SspMI CTAG 1 cut(s) 1055
SstI GAGCTC 2 cut(s) 669, 764
StyD4I CCNGG 5 cut(s) 294, 634, 682, 825, 1074
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 3 cut(s) 94, 350, 515
TaiI ACGT 2 cut(s) 44, 255
TaqI TCGA 2 cut(s) 18, 1008
TasI AATT 7 cut(s) 105, 119, 146, 454, 780, 796, 841
TatI WGTACW 1 cut(s) 1117
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 2 cut(s) 197, 1035
Tru1I TTAA 2 cut(s) 122, 768
Tru9I TTAA 2 cut(s) 122, 768
TscAI CASTG 1 cut(s) 931
TseFI GTSAC 2 cut(s) 572, 995
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 2 cut(s) 572, 995
TspDTI ATGAA 5 cut(s) 23, 139, 159, 781, 1027
TspGWI ACGGA 2 cut(s) 49, 514
TspRI CASTG 1 cut(s) 931
Van91I CCANNNNNTGG 1 cut(s) 1021
VpaK11BI GGWCC 2 cut(s) 522, 638
VspI ATTAAT 1 cut(s) 768
XapI RAATTY 1 cut(s) 146
XbaI TCTAGA 1 cut(s) 1054
XcmI CCANNNNNNNNNTGG 2 cut(s) 91, 516
XmnI GAANNNNTTC 1 cut(s) 14
XspI CTAG 1 cut(s) 1055
ZrmI AGTACT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.