FvH4_3g42702

Retroviral aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001157
Physical Location & Seq
Reverse (-)
35666444 .. 35667322
879 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42702.t1

Sequence Viewer

Length: 879 bp
ATGGCATGCCTCGTTTCATCGTCAGCGATCGTGACCGCATCTTCATCAGCCAATTCTGGGAGCACTTCTTCAAGCTCCAAGGCACCCAACTTTGTCGCAGCTCCGCCTATCACCCACAAAACCGATGGCCAATCAGAGGTGCTAAATCGCACACTAGAACACTACCTCCGGTGTTTCATCAATGACAAACCAACCAATTGGGCGAACCTCCTCCACTGGGCAGAGTGGTGGTACAATTCCACCTATCATGCCACCATCAAAATGACTCCATTCGAGGCGGTATACGGTTACCCATCCCCTGCTATCACCCGCTATCTTCCGGGCTCAACCTCAGTCGGCTCCGTGGACAAGAATTTGAAAAACCGCGACTCTCTGCTTGCAACCTTGAAGAGCCACATACATTCGGCCCAAAACCGGATGAAGCAACACTCTGATAAGAAGAGGACTGAGCGCGACTTTATCGAGGGTGACTTGGTCTTCCTCAAATTGCACCCATACCGCCTACAATCTGTGGTCCGCCGTCCAGCCCACAAGCTTGCTCCTCGTTTCTATGGACCATTCCAGATCGCTGCACGAATCGGGAAGGTCGCCTACAAACTCAGCTTGCCTGCCAACTCCAAGATCCACCCCATCTTCCATGTGTCTTTGCTTAAGTGCCGGATTGGAGATCAGACACCGGTCGCCACCACACTTCCTCAGTTTGACAATAGTGGCGCTCTCACCTGGGAACCAGAGCGCGTGGTGGATATGCACATCGTCAAGAAGAAAAGACGTTCAGTTACAGAATGGTTAGTGGTTTGGGCAGGGCTCCCAGCTGATGATGCCACGTGGGAAGAGGCTTACTCTATCATGAAAAAGTTTCCCACCTTCCAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2

Protein Analysis

293

Amino Acids

32.88

Weight (kDa)

9.7

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_Tf2-1 PF24626 156 - 218 4.1e-23 Tf2-1-like, SH3 domain
Chromo PF00385 242 - 289 9.3e-08 Chromo (CHRromatin Organisation MOdifier) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 282
AccII CGCG 3 cut(s) 366, 453, 738
AciI CCGC 7 cut(s) 36, 104, 278, 310, 364, 499, 517
AclWI GGATC 1 cut(s) 616
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 352
AcvI CACGTG 1 cut(s) 828
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 4 cut(s) 57, 136, 217, 414
AflII CTTAAG 1 cut(s) 650
AgeI ACCGGT 1 cut(s) 676
AgsI TTSAA 3 cut(s) 72, 358, 388
AjnI CCWGG 1 cut(s) 722
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 6 cut(s) 75, 101, 535, 603, 815, 875
AluI AGCT 6 cut(s) 75, 101, 535, 603, 815, 875
Alw21I GWGCWC 1 cut(s) 65
AlwI GGATC 1 cut(s) 616
AoxI GGCC 2 cut(s) 127, 405
ApeKI GCWGC 2 cut(s) 98, 569
ApoI RAATTY 1 cut(s) 352
AsiGI ACCGGT 1 cut(s) 676
AspLEI GCGC 3 cut(s) 453, 716, 738
AspS9I GGNCC 3 cut(s) 406, 514, 554
AsuC2I CCSGG 1 cut(s) 321
AsuHPI GGTGA 4 cut(s) 103, 298, 479, 712
AvaII GGWCC 2 cut(s) 514, 554
BalI TGGCCA 1 cut(s) 129
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 2 cut(s) 326, 810
BarI GAAGNNNNNNTAC 2 cut(s) 575, 607
BbrPI CACGTG 1 cut(s) 828
BbsI GAAGAC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 110, 556
BccI CCATC 4 cut(s) 119, 263, 301, 638
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 1 cut(s) 724
BcnI CCSGG 1 cut(s) 321
BfaI CTAG 1 cut(s) 155
BfoI RGCGCY 1 cut(s) 717
BfrI CTTAAG 1 cut(s) 650
BisI GCNGC 2 cut(s) 99, 570
BlsI GCNGC 2 cut(s) 100, 571
Bme1390I CCNGG 2 cut(s) 321, 724
Bme18I GGWCC 2 cut(s) 514, 554
BmgT120I GGNCC 3 cut(s) 406, 514, 554
BmiI GGNNCC 4 cut(s) 84, 340, 729, 809
BmrFI CCNGG 2 cut(s) 321, 724
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 2 cut(s) 47, 811
BmuI ACTGGG 1 cut(s) 226
BoxI GACNNNNGTC 1 cut(s) 677
BpiI GAAGAC 1 cut(s) 469
BplI GAGNNNNNCTC 2 cut(s) 827, 859
BpuMI CCSGG 1 cut(s) 321
BsaAI YACGTR 1 cut(s) 828
BsaJI CCNNGG 3 cut(s) 78, 342, 723
BsaWI WCCGGW 3 cut(s) 168, 414, 676
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 136, 217, 414
Bse118I RCCGGY 1 cut(s) 676
Bse1I ACTGG 1 cut(s) 221
BseBI CCWGG 1 cut(s) 724
BseDI CCNNGG 3 cut(s) 78, 342, 723
BseGI GGATG 2 cut(s) 293, 423
BseLI CCNNNNNNNGG 4 cut(s) 57, 136, 217, 414
BseMII CTCAG 4 cut(s) 345, 438, 613, 710
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 2 cut(s) 200, 531
BseXI GCAGC 2 cut(s) 110, 556
BseYI CCCAGC 1 cut(s) 811
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 3 cut(s) 366, 453, 738
Bsh1285I CGRYCG 2 cut(s) 30, 681
BshFI GGCC 2 cut(s) 129, 407
BshNI GGYRCC 1 cut(s) 82
BshTI ACCGGT 1 cut(s) 676
BsiEI CGRYCG 2 cut(s) 30, 681
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 5 cut(s) 169, 320, 415, 658, 677
BslI CCNNNNNNNGG 4 cut(s) 57, 136, 217, 414
BsnI GGCC 2 cut(s) 129, 407
Bsp1286I GDGCHC 3 cut(s) 65, 326, 810
Bsp143I GATC 4 cut(s) 27, 564, 621, 667
BspACI CCGC 7 cut(s) 36, 104, 278, 310, 364, 499, 517
BspANI GGCC 2 cut(s) 129, 407
BspCNI CTCAG 4 cut(s) 344, 439, 612, 709
BspFNI CGCG 3 cut(s) 366, 453, 738
BspHI TCATGA 1 cut(s) 849
BspLI GGNNCC 4 cut(s) 84, 340, 729, 809
BspPI GGATC 1 cut(s) 616
BspQI GCTCTTC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 650
BsrFI RCCGGY 1 cut(s) 676
BsrI ACTGG 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 676
BssECI CCNNGG 3 cut(s) 78, 342, 723
BssMI GATC 4 cut(s) 27, 564, 621, 667
BssNAI GTATAC 1 cut(s) 283
BssT1I CCWWGG 1 cut(s) 78
Bst1107I GTATAC 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 724
Bst4CI ACNGT 1 cut(s) 287
Bst6I CTCTTC 3 cut(s) 383, 434, 828
BstAFI CTTAAG 1 cut(s) 650
BstBAI YACGTR 1 cut(s) 828
BstC8I GCNNGC 5 cut(s) 7, 378, 537, 605, 609
BstDEI CTNAG 4 cut(s) 331, 447, 599, 696
BstDSI CCRYGG 1 cut(s) 342
BstEII GGTNACC 1 cut(s) 287
BstF5I GGATG 2 cut(s) 293, 423
BstFNI CGCG 3 cut(s) 366, 453, 738
BstH2I RGCGCY 1 cut(s) 717
BstHHI GCGC 3 cut(s) 453, 716, 738
BstKTI GATC 4 cut(s) 30, 567, 624, 670
BstMBI GATC 4 cut(s) 27, 564, 621, 667
BstMCI CGRYCG 2 cut(s) 30, 681
BstMWI GCNNNNNNNGC 1 cut(s) 821
BstNI CCWGG 1 cut(s) 724
BstNSI RCATGY 1 cut(s) 9
BstPAI GACNNNNGTC 1 cut(s) 677
BstPI GGTNACC 1 cut(s) 287
BstSCI CCNGG 2 cut(s) 319, 722
BstUI CGCG 3 cut(s) 366, 453, 738
BstV1I GCAGC 2 cut(s) 110, 556
BstV2I GAAGAC 1 cut(s) 469
BstX2I RGATCY 1 cut(s) 621
BstXI CCANNNNNNTGG 1 cut(s) 198
BstYI RGATCY 1 cut(s) 621
BstZ17I GTATAC 1 cut(s) 283
BsuRI GGCC 2 cut(s) 129, 407
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 2 cut(s) 293, 423
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 5 cut(s) 7, 378, 537, 605, 609
CciI TCATGA 1 cut(s) 849
CfoI GCGC 3 cut(s) 453, 716, 738
Cfr10I RCCGGY 1 cut(s) 676
Cfr13I GGNCC 3 cut(s) 406, 514, 554
Csp6I GTAC 1 cut(s) 232
CspAI ACCGGT 1 cut(s) 676
CviAII CATG 4 cut(s) 6, 248, 638, 850
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 4 cut(s) 331, 447, 599, 696
DpnI GATC 4 cut(s) 29, 566, 623, 669
DpnII GATC 4 cut(s) 27, 564, 621, 667
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 3 cut(s) 383, 434, 828
EarI CTCTTC 3 cut(s) 383, 434, 828
EciI GGCGGA 2 cut(s) 93, 506
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 326, 810
Eco47I GGWCC 2 cut(s) 514, 554
Eco72I CACGTG 1 cut(s) 828
Eco91I GGTNACC 1 cut(s) 287
EcoO65I GGTNACC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 722
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 2 cut(s) 326, 810
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 4 cut(s) 9, 251, 641, 853
FaiI YATR 9 cut(s) 7, 249, 283, 398, 496, 552, 639, 749, 851
FatI CATG 4 cut(s) 5, 247, 637, 849
FauI CCCGC 1 cut(s) 317
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 99, 570
FokI GGATG 2 cut(s) 280, 430
FriOI GRGCYC 2 cut(s) 326, 810
Fsp4HI GCNGC 2 cut(s) 99, 570
FspBI CTAG 1 cut(s) 155
GlaI GCGC 3 cut(s) 452, 715, 737
GluI GCNGC 2 cut(s) 99, 570
GsaI CCCAGC 1 cut(s) 815
HaeII RGCGCY 1 cut(s) 717
HaeIII GGCC 2 cut(s) 129, 407
HapII CCGG 5 cut(s) 169, 320, 415, 658, 677
HhaI GCGC 3 cut(s) 453, 716, 738
Hin1II CATG 4 cut(s) 9, 251, 641, 853
Hin6I GCGC 3 cut(s) 451, 714, 736
HinP1I GCGC 3 cut(s) 451, 714, 736
HindIII AAGCTT 2 cut(s) 533, 873
HinfI GANTC 3 cut(s) 265, 368, 576
HpaII CCGG 5 cut(s) 169, 320, 415, 658, 677
HphI GGTGA 4 cut(s) 103, 298, 479, 712
Hpy166II GTNNAC 2 cut(s) 283, 346
Hpy188I TCNGA 3 cut(s) 136, 433, 672
Hpy188III TCNNGA 5 cut(s) 31, 562, 580, 760, 850
Hpy8I GTNNAC 2 cut(s) 283, 346
HpyAV CCTTC 2 cut(s) 577, 877
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 2 cut(s) 772, 827
HpyCH4V TGCA 4 cut(s) 380, 490, 572, 751
HpyF10VI GCNNNNNNNGC 1 cut(s) 821
HpyF3I CTNAG 4 cut(s) 331, 447, 599, 696
HpySE526I ACGT 2 cut(s) 772, 827
Hsp92II CATG 4 cut(s) 9, 251, 641, 853
HspAI GCGC 3 cut(s) 451, 714, 736
Kzo9I GATC 4 cut(s) 27, 564, 621, 667
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 6 cut(s) 60, 80, 106, 344, 544, 813
Lsp1109I GCAGC 2 cut(s) 110, 556
LweI GCATC 2 cut(s) 47, 811
MaeI CTAG 1 cut(s) 155
MaeII ACGT 2 cut(s) 772, 827
MaeIII GTNAC 4 cut(s) 31, 287, 467, 778
MalI GATC 4 cut(s) 29, 566, 623, 669
MboI GATC 4 cut(s) 27, 564, 621, 667
MboII GAAGA 9 cut(s) 33, 60, 308, 400, 451, 469, 625, 775, 845
MfeI CAATTG 1 cut(s) 196
MflI RGATCY 1 cut(s) 621
MhlI GDGCHC 3 cut(s) 65, 326, 810
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 5 cut(s) 52, 196, 235, 352, 485
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 2 cut(s) 259, 362
Mox20I TGGCCA 1 cut(s) 129
MscI TGGCCA 1 cut(s) 129
MseI TTAA 1 cut(s) 651
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 815
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 5 cut(s) 169, 320, 415, 658, 677
MspR9I CCNGG 2 cut(s) 321, 724
MunI CAATTG 1 cut(s) 196
MvaI CCWGG 1 cut(s) 724
MvnI CGCG 3 cut(s) 366, 453, 738
MwoI GCNNNNNNNGC 1 cut(s) 821
NciI CCSGG 1 cut(s) 321
NdeII GATC 4 cut(s) 27, 564, 621, 667
NlaIII CATG 4 cut(s) 9, 251, 641, 853
NlaIV GGNNCC 4 cut(s) 84, 340, 729, 809
NmuCI GTSAC 2 cut(s) 31, 467
NspI RCATGY 1 cut(s) 9
PaeI GCATGC 1 cut(s) 9
PagI TCATGA 1 cut(s) 849
PciSI GCTCTTC 1 cut(s) 383
PfeI GAWTC 1 cut(s) 576
PflFI GACNNNGTC 1 cut(s) 473
PinAI ACCGGT 1 cut(s) 676
PkrI GCNGC 2 cut(s) 100, 571
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 2 cut(s) 259, 362
PmaCI CACGTG 1 cut(s) 828
PmlI CACGTG 1 cut(s) 828
PpsI GAGTC 2 cut(s) 259, 362
Ppu21I YACGTR 1 cut(s) 828
PshAI GACNNNNGTC 1 cut(s) 677
Psp6I CCWGG 1 cut(s) 722
PspCI CACGTG 1 cut(s) 828
PspEI GGTNACC 1 cut(s) 287
PspFI CCCAGC 1 cut(s) 811
PspGI CCWGG 1 cut(s) 722
PspN4I GGNNCC 4 cut(s) 84, 340, 729, 809
PspPI GGNCC 3 cut(s) 406, 514, 554
PsuI RGATCY 1 cut(s) 621
PsyI GACNNNGTC 1 cut(s) 473
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 2 cut(s) 99, 570
Sau3AI GATC 4 cut(s) 27, 564, 621, 667
Sau96I GGNCC 3 cut(s) 406, 514, 554
SchI GAGTC 2 cut(s) 259, 362
ScrFI CCNGG 2 cut(s) 321, 724
SduI GDGCHC 3 cut(s) 65, 326, 810
SfaNI GCATC 2 cut(s) 47, 811
SinI GGWCC 2 cut(s) 514, 554
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
SphI GCATGC 1 cut(s) 9
Sse9I AATT 5 cut(s) 52, 196, 235, 352, 485
SsiI CCGC 7 cut(s) 36, 104, 278, 310, 364, 499, 517
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 2 cut(s) 319, 722
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 2 cut(s) 775, 830
TaqI TCGA 2 cut(s) 273, 462
TasI AATT 5 cut(s) 52, 196, 235, 352, 485
TfiI GAWTC 1 cut(s) 576
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 1 cut(s) 221
TseFI GTSAC 2 cut(s) 31, 467
TseI GCWGC 2 cut(s) 98, 569
Tsp45I GTSAC 2 cut(s) 31, 467
TspDTI ATGAA 5 cut(s) 6, 33, 166, 434, 866
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 1 cut(s) 221
Tth111I GACNNNGTC 1 cut(s) 473
Vha464I CTTAAG 1 cut(s) 650
VpaK11BI GGWCC 2 cut(s) 514, 554
XapI RAATTY 1 cut(s) 352
XceI RCATGY 1 cut(s) 9
XcmI CCANNNNNNNNNTGG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.