RLG00000002118

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001157
Physical Location & Seq
Forward (+)
25797893 .. 25798523
631 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002118

Sequence Viewer

Length: 357 bp
ATGCGTTTACAAGGTTCAATTGACAACGTTCCCATCCGAGTCTTCGTCAATTGTGGAGTTGCTATGAACTTTCTCAATCCGAAAATTATGGAAAAGCTGGGTATCCCTATACAACCACACCCTTCTCCTCTTTTCACCTCTGCATCTATTCATATAGTGACGCCGGAAGGTATAACTCACAATATAACAGTTCACATTCAAGGTTACTATTTTACCTCATCATTTTTGCTTCTTCCTGTGGATGGTTGTGATTTAGTCCTTGGTGGCCAGTGGTTAGACACACTAGGCTTTATTGGCTGGCATTTTCTTGACAAGGTCATGGTTTTCACGGTCGACGGCAAGCAACATGTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

119

Amino Acids

13.12

Weight (kDa)

6.25

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVP_2 PF08284 7 - 93 1.4e-06 Retroviral aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 333
AclI AACGTT 1 cut(s) 27
AcoI YGGCCR 1 cut(s) 265
AcyI GRCGYC 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 2 cut(s) 18, 200
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
AoxI GGCC 1 cut(s) 265
AsuHPI GGTGA 1 cut(s) 127
BalI TGGCCA 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 34
BccI CCATC 2 cut(s) 41, 236
BceAI ACGGC 1 cut(s) 352
BciVI GTATCC 1 cut(s) 113
BfaI CTAG 1 cut(s) 284
BfuI GTATCC 1 cut(s) 113
BmsI GCATC 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 34
BsaHI GRCGYC 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 259
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 2 cut(s) 33, 247
BseLI CCNNNNNNNGG 1 cut(s) 242
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 1 cut(s) 117
BseYI CCCAGC 1 cut(s) 97
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 1 cut(s) 267
BsiEI CGRYCG 1 cut(s) 333
BsiSI CCGG 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 242
BsnI GGCC 1 cut(s) 267
BspANI GGCC 1 cut(s) 267
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 259
BssNI GRCGYC 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 259
Bst4CI ACNGT 2 cut(s) 190, 331
BstACI GRCGYC 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 299, 341
BstF5I GGATG 2 cut(s) 33, 247
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 350
BstV2I GAAGAC 1 cut(s) 34
BsuI GTATCC 1 cut(s) 113
BsuRI GGCC 1 cut(s) 267
BtsCI GGATG 2 cut(s) 33, 247
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 299, 341
CseI GACGC 1 cut(s) 169
CviAII CATG 2 cut(s) 319, 347
CviJI RGCY 4 cut(s) 97, 267, 288, 297
CviKI_1 RGCY 4 cut(s) 97, 267, 288, 297
EaeI YGGCCR 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 2 cut(s) 322, 350
FatI CATG 2 cut(s) 318, 346
FblI GTMKAC 1 cut(s) 333
FokI GGATG 2 cut(s) 20, 254
FspBI CTAG 1 cut(s) 284
GsaI CCCAGC 1 cut(s) 101
HaeIII GGCC 1 cut(s) 267
HapII CCGG 1 cut(s) 164
HgaI GACGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 161
Hin1II CATG 2 cut(s) 322, 350
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 1 cut(s) 39
HpaII CCGG 1 cut(s) 164
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 3 cut(s) 8, 193, 334
Hpy188I TCNGA 2 cut(s) 38, 81
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 3 cut(s) 8, 193, 334
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 2 cut(s) 132, 161
HpyCH4III ACNGT 2 cut(s) 190, 331
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 143
HpyF10VI GCNNNNNNNGC 1 cut(s) 294
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 161
Hsp92II CATG 2 cut(s) 322, 350
LpnPI CCDG 5 cut(s) 83, 177, 249, 281, 283
LweI GCATC 1 cut(s) 152
MaeI CTAG 1 cut(s) 284
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 157, 203
MboII GAAGA 2 cut(s) 34, 224
MfeI CAATTG 2 cut(s) 18, 49
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 3 cut(s) 18, 49, 84
MluNI TGGCCA 1 cut(s) 267
MlyI GAGTC 1 cut(s) 48
MnlI CCTC 3 cut(s) 138, 148, 226
Mox20I TGGCCA 1 cut(s) 267
MscI TGGCCA 1 cut(s) 267
Msp20I TGGCCA 1 cut(s) 267
MspI CCGG 1 cut(s) 164
MunI CAATTG 2 cut(s) 18, 49
MwoI GCNNNNNNNGC 1 cut(s) 294
NlaIII CATG 2 cut(s) 322, 350
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 350
PciI ACATGT 1 cut(s) 346
PflFI GACNNNGTC 1 cut(s) 314
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PscI ACATGT 1 cut(s) 346
Psp1406I AACGTT 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 97
PsyI GACNNNGTC 1 cut(s) 314
SalI GTCGAC 1 cut(s) 332
SchI GAGTC 1 cut(s) 48
SetI ASST 8 cut(s) 16, 30, 99, 140, 172, 205, 218, 318
SfaNI GCATC 1 cut(s) 152
Sse9I AATT 3 cut(s) 18, 49, 84
SspMI CTAG 1 cut(s) 284
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 2 cut(s) 190, 331
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 333
TasI AATT 3 cut(s) 18, 49, 84
TscAI CASTG 1 cut(s) 275
TseFI GTSAC 1 cut(s) 157
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 2 cut(s) 80, 140
TspRI CASTG 1 cut(s) 275
Tth111I GACNNNGTC 1 cut(s) 314
XceI RCATGY 1 cut(s) 350
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.