Rw0G007090

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00266
OG0001157
Physical Location & Seq
Reverse (-)
23943 .. 26493
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007090.1

Sequence Viewer

Length: 1761 bp
ATGGGGGAGCGATACTTGAAGACCCAAAGAGCCCCTCTATTAGAAAAGGTAGCCATTGCAGCCTCCCATTTCGGTCCCGATGCGTGTTTTTGGATGAATTCATTAGAGCAAAGACACCCCGTCATTACTTGGGAACAATTTGTGCCAGCATTCTTGGCTCACTTCGGTGCCGGTGCAAACCAGGATTTCAAGATCGCACTATCCCACCTCCAACAAACTTCTACGGTGGAACTCTTCATCACAGCTTTCACCAAGTTATCTTACAGGGCTCCGGATTGGAATGACAATCAACTTCTTCCCATGTTTCTTGGCGGTTTGAAAACTGCAATCCGGCATGACGTCATGTCCTTCAACCCACTCACCCTGACAGAAGCCCAATGCCTAGCAAGGTGCTCAGAGGCCAAGTTGTCAGAAACTCCACGAGCTGCACGCCCCAATTCATGGTCCCATGCTTTTCGTACATCACCTTACCCACCCACTTCCTCATCCACGTACCGTCCTCACAACCCCATCCCACCAACTACACCCACCCAATCAACCACTCCACACAACCCCTCACTGACCAAACCCACAACCAGCCGATACCATCGCATCTTGTCTACTGCAGAACAACGTGAGAGAAGGGCCAAAGGACTGTGCTTTAATTGTGAAGAGCTATACTTACCTACCCATGTCTGCAAAAGGCCATTCCTGGCCATACTTGCAGTGGATTTAACTGAGGAAGATCTTCCTGATAATCTGGAATTTCATGATTGTATGCCGACTCATGAGGAACCCACTCCTGACACAGGCGAATCATACCCACTCCATGTCATAACCACCCCCACCATTGGAGAGGTCATGAAGATTAAAGGTATGGTTAATGGGTCTTCGGCCACAATTTTCATCGATTGTGGGGCCGCCTCGAATTTCTTGAATCCCCAATTGGCTCGGAATCTAGGATTGCCCATTACACCAAGCTCTGACTCCAGATTTGCCTCCGCCTCAGGCGAAACACTAGTGCCTAGTGGCACTGCTAGTAACGTCGCTGTACTCATTCAAGGATATGCTATCACTGGCTCATTCTTATTGTTGCCAGTAGCGAGCTGCGACCTGGTATTGGGAGCTCCGTGGTTGAACTCTCTTGGTCTAATTGGCTGGCATTTTCAAGAGAAGGTGATGATGTTCTATACTGATGGCCAATGCCATATCCTTCGAGGAATCACACAGCAACCTTCCCTTCACTCTCCATCGGAGATCCTAACTCTTCTATCCACTGACCAATGGGATCCTTTAGCCCAAAGCCCAACCTTACCATTACCGGCCCAAACTGAAGCTAACCATCCATCAATCCAAACCATTCTCACCTCTTTTTCGGATGTGTTCTCTACACCCACTGGCCTTCCACCTAAGCGACCCATTGACCATCAAATCACCCTATTACCCAACACAAGCCCAGTTAACGTCAGACCCTATAGATACCCACACTCCCAAAAAGCTGAAATTGAAGCCCAAGTAACCGACATGCTTAGCCAAGGCATTATACGACGGAGTTGCAACCTTTTTTCTTCTCCGGTCCTCTTAGTGAAGAAGAAAGAAGGGACATGGCGGTTTTGTGTGGACTATCAATCACTCAATGCGGTAACTGTCAAAGATCAATTCCCCATTCCGGTGGTAGATGAGCTTTTGGATGAACTCCATGGTGCCACAATTTTTTCCAAACTCGACTTAAGGTCCGGCTACCACCAAATCCGTATGCATGAGCTAGACATCCCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

586

Amino Acids

65.06

Weight (kDa)

6.34

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 15 - 130 1e-09 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 19 - 107 2.4e-12 Retrotransposon gag protein
RVP_2 PF08284 279 - 375 7.1e-06 Retroviral aspartyl protease
gag-asp_proteas PF13975 283 - 372 1.9e-07 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 284 - 344 6.6e-07 Aspartyl protease
RVT_1 PF00078 522 - 584 4.2e-09 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 342
AccB1I GGYRCC 2 cut(s) 167, 1682
AccB7I CCANNNNNTGG 1 cut(s) 441
AccI GTMKAC 1 cut(s) 599
AccIII TCCGGA 1 cut(s) 271
AciI CCGC 5 cut(s) 312, 900, 981, 1588, 1619
AclWI GGATC 3 cut(s) 1231, 1262, 1275
AcoI YGGCCR 3 cut(s) 693, 873, 1177
AcsI RAATTY 3 cut(s) 97, 743, 907
AcuI CTGAAG 1 cut(s) 1332
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 3 cut(s) 460, 494, 1032
AfiI CCNNNNNNNGG 5 cut(s) 441, 788, 830, 1099, 1648
AflII CTTAAG 1 cut(s) 1708
AgsI TTSAA 9 cut(s) 19, 190, 319, 352, 916, 1040, 1117, 1148, 1487
AhdI GACNNNNNGTC 3 cut(s) 119, 343, 1711
AhlI ACTAGT 1 cut(s) 997
AjnI CCWGG 3 cut(s) 180, 690, 1092
AjuI GAANNNNNNNTTGG 2 cut(s) 908, 940
AleI CACNNNNGTG 1 cut(s) 165
Alw21I GWGCWC 2 cut(s) 395, 1108
AlwI GGATC 3 cut(s) 1231, 1262, 1275
Aor13HI TCCGGA 1 cut(s) 271
AoxI GGCC 9 cut(s) 399, 624, 683, 693, 873, 897, 1177, 1302, 1378
ApeKI GCWGC 3 cut(s) 59, 425, 1086
ApoI RAATTY 3 cut(s) 97, 743, 907
ArsI GACNNNNNNTTYG 2 cut(s) 956, 988
Asp700I GAANNNNTTC 1 cut(s) 726
AspS9I GGNCC 7 cut(s) 74, 444, 624, 897, 1303, 1555, 1713
AsuHPI GGTGA 6 cut(s) 241, 352, 456, 1168, 1336, 1405
AvaII GGWCC 4 cut(s) 74, 444, 1555, 1713
AxyI CCTNAGG 1 cut(s) 985
BalI TGGCCA 2 cut(s) 695, 1179
BamHI GGATCC 1 cut(s) 1267
BanI GGYRCC 2 cut(s) 167, 1682
BanII GRGCYC 3 cut(s) 34, 271, 1108
BauI CACGAG 1 cut(s) 420
BbsI GAAGAC 2 cut(s) 26, 861
Bbv12I GWGCWC 2 cut(s) 395, 1108
BbvI GCAGC 3 cut(s) 71, 412, 1073
BccI CCATC 7 cut(s) 518, 594, 1169, 1237, 1329, 1333, 1413
BcgI CGANNNNNNTGC 2 cut(s) 1515, 1549
BciT130I CCWGG 3 cut(s) 182, 692, 1094
BcuI ACTAGT 1 cut(s) 997
BfaI CTAG 6 cut(s) 383, 938, 998, 1005, 1017, 1745
BfmI CTRYAG 2 cut(s) 603, 1453
BfrI CTTAAG 1 cut(s) 1708
BglII AGATCT 1 cut(s) 724
BisI GCNGC 4 cut(s) 60, 426, 900, 1087
BlpI GCTNAGC 1 cut(s) 1508
BlsI GCNGC 4 cut(s) 61, 427, 901, 1088
Bme1390I CCNGG 3 cut(s) 182, 692, 1094
Bme18I GGWCC 4 cut(s) 74, 444, 1555, 1713
BmeRI GACNNNNNGTC 3 cut(s) 119, 343, 1711
BmgT120I GGNCC 7 cut(s) 74, 444, 624, 897, 1303, 1555, 1713
BmiI GGNNCC 8 cut(s) 76, 169, 270, 446, 774, 898, 1269, 1684
BmrFI CCNGG 3 cut(s) 182, 692, 1094
BmrI ACTGGG 1 cut(s) 1430
BmsI GCATC 2 cut(s) 70, 600
BmuI ACTGGG 1 cut(s) 1430
BpiI GAAGAC 2 cut(s) 26, 861
BpmI CTGGAG 1 cut(s) 952
Bpu10I CCTNAGC 1 cut(s) 1389
Bpu1102I GCTNAGC 1 cut(s) 1508
Bsa29I ATCGAT 1 cut(s) 888
BsaAI YACGTR 1 cut(s) 492
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 1109, 1513, 1678
BsaWI WCCGGW 3 cut(s) 271, 1552, 1648
BsaXI ACNNNNNCTCC 2 cut(s) 1451, 1481
Bsc4I CCNNNNNNNGG 5 cut(s) 441, 788, 830, 1099, 1648
Bse118I RCCGGY 2 cut(s) 170, 1300
Bse1I ACTGG 4 cut(s) 1060, 1076, 1381, 1436
Bse21I CCTNAGG 1 cut(s) 985
Bse3DI GCAATG 1 cut(s) 54
BseAI TCCGGA 1 cut(s) 271
BseBI CCWGG 3 cut(s) 182, 692, 1094
BseCI ATCGAT 1 cut(s) 888
BseDI CCNNGG 3 cut(s) 1109, 1513, 1678
BseGI GGATG 7 cut(s) 99, 485, 510, 1321, 1363, 1675, 1749
BseLI CCNNNNNNNGG 5 cut(s) 441, 788, 830, 1099, 1648
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 3 cut(s) 408, 708, 999
BseNI ACTGG 4 cut(s) 1060, 1076, 1381, 1436
BseXI GCAGC 3 cut(s) 71, 412, 1073
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 9 cut(s) 401, 626, 685, 695, 875, 899, 1179, 1304, 1380
BshNI GGYRCC 2 cut(s) 167, 1682
BshVI ATCGAT 1 cut(s) 888
BsiHKAI GWGCWC 2 cut(s) 395, 1108
BsiSI CCGG 7 cut(s) 171, 272, 331, 1301, 1553, 1649, 1716
BslFI GGGAC 3 cut(s) 60, 430, 1594
BslI CCNNNNNNNGG 5 cut(s) 441, 788, 830, 1099, 1648
BsmFI GGGAC 3 cut(s) 60, 430, 1594
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 9 cut(s) 401, 626, 685, 695, 875, 899, 1179, 1304, 1380
Bsp1286I GDGCHC 4 cut(s) 34, 271, 395, 1108
Bsp13I TCCGGA 1 cut(s) 271
Bsp143I GATC 5 cut(s) 192, 724, 1236, 1267, 1633
Bsp1720I GCTNAGC 1 cut(s) 1508
Bsp19I CCATGG 1 cut(s) 1678
BspACI CCGC 5 cut(s) 312, 900, 981, 1588, 1619
BspANI GGCC 9 cut(s) 401, 626, 685, 695, 875, 899, 1179, 1304, 1380
BspCNI CTCAG 3 cut(s) 407, 709, 998
BspDI ATCGAT 1 cut(s) 888
BspEI TCCGGA 1 cut(s) 271
BspHI TCATGA 3 cut(s) 748, 766, 840
BspLI GGNNCC 8 cut(s) 76, 169, 270, 446, 774, 898, 1269, 1684
BspMAI CTGCAG 1 cut(s) 607
BspPI GGATC 3 cut(s) 1231, 1262, 1275
BspQI GCTCTTC 1 cut(s) 645
BspT107I GGYRCC 2 cut(s) 167, 1682
BspTI CTTAAG 1 cut(s) 1708
BsrDI GCAATG 1 cut(s) 54
BsrFI RCCGGY 2 cut(s) 170, 1300
BsrI ACTGG 4 cut(s) 1060, 1076, 1381, 1436
BssAI RCCGGY 2 cut(s) 170, 1300
BssECI CCNNGG 3 cut(s) 1109, 1513, 1678
BssMI GATC 5 cut(s) 192, 724, 1236, 1267, 1633
BssNI GRCGYC 1 cut(s) 339
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 2 cut(s) 1513, 1678
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 3 cut(s) 182, 692, 1094
Bst4CI ACNGT 4 cut(s) 226, 497, 636, 1627
Bst6I CTCTTC 3 cut(s) 239, 645, 1251
BstACI GRCGYC 1 cut(s) 339
BstAFI CTTAAG 1 cut(s) 1708
BstBAI YACGTR 1 cut(s) 492
BstC8I GCNNGC 4 cut(s) 147, 430, 1084, 1139
BstDEI CTNAG 6 cut(s) 394, 717, 985, 1389, 1508, 1561
BstDSI CCRYGG 2 cut(s) 1109, 1678
BstENI CCTNNNNNAGG 1 cut(s) 786
BstF5I GGATG 7 cut(s) 99, 485, 510, 1321, 1363, 1675, 1749
BstKTI GATC 5 cut(s) 195, 727, 1239, 1270, 1636
BstMBI GATC 5 cut(s) 192, 724, 1236, 1267, 1633
BstMWI GCNNNNNNNGC 3 cut(s) 59, 155, 701
BstNI CCWGG 3 cut(s) 182, 692, 1094
BstNSI RCATGY 1 cut(s) 1507
BstSCI CCNGG 3 cut(s) 180, 690, 1092
BstSFI CTRYAG 2 cut(s) 603, 1453
BstV1I GCAGC 3 cut(s) 71, 412, 1073
BstV2I GAAGAC 2 cut(s) 26, 861
BstX2I RGATCY 3 cut(s) 724, 1236, 1267
BstXI CCANNNNNNTGG 1 cut(s) 1651
BstYI RGATCY 3 cut(s) 724, 1236, 1267
Bsu15I ATCGAT 1 cut(s) 888
Bsu36I CCTNAGG 1 cut(s) 985
BsuRI GGCC 9 cut(s) 401, 626, 685, 695, 875, 899, 1179, 1304, 1380
BsuTUI ATCGAT 1 cut(s) 888
BtgI CCRYGG 2 cut(s) 1109, 1678
BtgZI GCGATG 1 cut(s) 572
BtsCI GGATG 7 cut(s) 99, 485, 510, 1321, 1363, 1675, 1749
BtsI GCAGTG 2 cut(s) 711, 1011
BtsIMutI CAGTG 6 cut(s) 557, 711, 1011, 1053, 1254, 1374
Cac8I GCNNGC 4 cut(s) 147, 430, 1084, 1139
CciI TCATGA 3 cut(s) 748, 766, 840
Cfr10I RCCGGY 2 cut(s) 170, 1300
Cfr13I GGNCC 7 cut(s) 74, 444, 624, 897, 1303, 1555, 1713
ClaI ATCGAT 1 cut(s) 888
CsiI ACCWGGT 1 cut(s) 1092
Csp6I GTAC 3 cut(s) 459, 493, 1031
CviQI GTAC 3 cut(s) 459, 493, 1031
DdeI CTNAG 6 cut(s) 394, 717, 985, 1389, 1508, 1561
DpnI GATC 5 cut(s) 194, 726, 1238, 1269, 1635
DpnII GATC 5 cut(s) 192, 724, 1236, 1267, 1633
DriI GACNNNNNGTC 3 cut(s) 119, 343, 1711
EaeI YGGCCR 3 cut(s) 693, 873, 1177
Eam1104I CTCTTC 3 cut(s) 239, 645, 1251
Eam1105I GACNNNNNGTC 3 cut(s) 119, 343, 1711
EarI CTCTTC 3 cut(s) 239, 645, 1251
EciI GGCGGA 1 cut(s) 970
Ecl136II GAGCTC 1 cut(s) 1106
Eco130I CCWWGG 2 cut(s) 1513, 1678
Eco24I GRGCYC 3 cut(s) 34, 271, 1108
Eco47I GGWCC 4 cut(s) 74, 444, 1555, 1713
Eco53kI GAGCTC 1 cut(s) 1106
Eco57I CTGAAG 1 cut(s) 1332
Eco81I CCTNAGG 1 cut(s) 985
EcoICRI GAGCTC 1 cut(s) 1106
EcoNI CCTNNNNNAGG 1 cut(s) 786
EcoRI GAATTC 1 cut(s) 97
EcoRII CCWGG 3 cut(s) 180, 690, 1092
EcoT14I CCWWGG 2 cut(s) 1513, 1678
EcoT22I ATGCAT 1 cut(s) 1740
EcoT38I GRGCYC 3 cut(s) 34, 271, 1108
ErhI CCWWGG 2 cut(s) 1513, 1678
FaqI GGGAC 3 cut(s) 60, 430, 1594
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 4 cut(s) 60, 426, 900, 1087
FokI GGATG 7 cut(s) 106, 472, 497, 1308, 1370, 1682, 1736
FriOI GRGCYC 3 cut(s) 34, 271, 1108
Fsp4HI GCNGC 4 cut(s) 60, 426, 900, 1087
FspBI CTAG 6 cut(s) 383, 938, 998, 1005, 1017, 1745
GluI GCNGC 4 cut(s) 60, 426, 900, 1087
GsuI CTGGAG 1 cut(s) 952
HaeIII GGCC 9 cut(s) 401, 626, 685, 695, 875, 899, 1179, 1304, 1380
HapII CCGG 7 cut(s) 171, 272, 331, 1301, 1553, 1649, 1716
Hin1I GRCGYC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 1441
HindII GTYRAC 1 cut(s) 1441
HinfI GANTC 6 cut(s) 763, 794, 916, 934, 965, 1200
HpaI GTTAAC 1 cut(s) 1441
HpaII CCGG 7 cut(s) 171, 272, 331, 1301, 1553, 1649, 1716
HphI GGTGA 6 cut(s) 241, 352, 456, 1168, 1336, 1405
Hpy166II GTNNAC 3 cut(s) 600, 1441, 1600
Hpy188I TCNGA 7 cut(s) 397, 412, 933, 964, 1234, 1357, 1448
Hpy8I GTNNAC 3 cut(s) 600, 1441, 1600
Hpy99I CGWCG 2 cut(s) 1028, 1530
HpyAV CCTTC 8 cut(s) 358, 615, 1147, 1202, 1224, 1229, 1391, 1571
HpyCH4III ACNGT 4 cut(s) 226, 497, 636, 1627
HpyCH4IV ACGT 5 cut(s) 339, 491, 613, 1023, 1443
HpyCH4V TGCA 9 cut(s) 59, 176, 326, 428, 605, 678, 704, 1536, 1738
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 155, 701
HpyF3I CTNAG 6 cut(s) 394, 717, 985, 1389, 1508, 1561
HpySE526I ACGT 5 cut(s) 339, 491, 613, 1023, 1443
Hsp92I GRCGYC 1 cut(s) 339
Kpn2I TCCGGA 1 cut(s) 271
KspAI GTTAAC 1 cut(s) 1441
Kzo9I GATC 5 cut(s) 192, 724, 1236, 1267, 1633
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 4 cut(s) 7, 274, 1103, 1111
Lsp1109I GCAGC 3 cut(s) 71, 412, 1073
LweI GCATC 2 cut(s) 70, 600
MabI ACCWGGT 1 cut(s) 1092
MaeI CTAG 6 cut(s) 383, 938, 998, 1005, 1017, 1745
MaeII ACGT 5 cut(s) 339, 491, 613, 1023, 1443
MaeIII GTNAC 3 cut(s) 1019, 1495, 1621
MalI GATC 5 cut(s) 194, 726, 1238, 1269, 1635
MboI GATC 5 cut(s) 192, 724, 1236, 1267, 1633
MfeI CAATTG 1 cut(s) 923
MflI RGATCY 3 cut(s) 724, 1236, 1267
MhlI GDGCHC 4 cut(s) 34, 271, 395, 1108
MlsI TGGCCA 2 cut(s) 695, 1179
MluNI TGGCCA 2 cut(s) 695, 1179
MlyI GAGTC 2 cut(s) 757, 959
MmeI TCCRAC 1 cut(s) 235
Mox20I TGGCCA 2 cut(s) 695, 1179
Mph1103I ATGCAT 1 cut(s) 1740
MroI TCCGGA 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 726
MscI TGGCCA 2 cut(s) 695, 1179
MseI TTAA 6 cut(s) 642, 713, 849, 861, 1440, 1709
MslI CAYNNNNRTG 2 cut(s) 165, 1649
Msp20I TGGCCA 2 cut(s) 695, 1179
MspCI CTTAAG 1 cut(s) 1708
MspI CCGG 7 cut(s) 171, 272, 331, 1301, 1553, 1649, 1716
MspR9I CCNGG 3 cut(s) 182, 692, 1094
MunI CAATTG 1 cut(s) 923
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 3 cut(s) 182, 692, 1094
MwoI GCNNNNNNNGC 3 cut(s) 59, 155, 701
NcoI CCATGG 1 cut(s) 1678
NdeII GATC 5 cut(s) 192, 724, 1236, 1267, 1633
NlaIV GGNNCC 8 cut(s) 76, 169, 270, 446, 774, 898, 1269, 1684
NsiI ATGCAT 1 cut(s) 1740
NspI RCATGY 1 cut(s) 1507
OliI CACNNNNGTG 1 cut(s) 165
PagI TCATGA 3 cut(s) 748, 766, 840
PciSI GCTCTTC 1 cut(s) 645
PctI GAATGC 1 cut(s) 149
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 4 cut(s) 794, 916, 934, 1200
PflMI CCANNNNNTGG 1 cut(s) 441
PkrI GCNGC 4 cut(s) 61, 427, 901, 1088
PleI GAGTC 2 cut(s) 757, 959
PpsI GAGTC 2 cut(s) 757, 959
Ppu21I YACGTR 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 1108
Psp6I CCWGG 3 cut(s) 180, 690, 1092
PspGI CCWGG 3 cut(s) 180, 690, 1092
PspN4I GGNNCC 8 cut(s) 76, 169, 270, 446, 774, 898, 1269, 1684
PspPI GGNCC 7 cut(s) 74, 444, 624, 897, 1303, 1555, 1713
PstI CTGCAG 1 cut(s) 607
PsuI RGATCY 3 cut(s) 724, 1236, 1267
RsaI GTAC 3 cut(s) 460, 494, 1032
RsaNI GTAC 3 cut(s) 459, 493, 1031
RseI CAYNNNNRTG 2 cut(s) 165, 1649
SacI GAGCTC 1 cut(s) 1108
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 6 cut(s) 642, 713, 849, 861, 1440, 1709
SatI GCNGC 4 cut(s) 60, 426, 900, 1087
Sau3AI GATC 5 cut(s) 192, 724, 1236, 1267, 1633
Sau96I GGNCC 7 cut(s) 74, 444, 624, 897, 1303, 1555, 1713
SchI GAGTC 2 cut(s) 757, 959
ScrFI CCNGG 3 cut(s) 182, 692, 1094
SduI GDGCHC 4 cut(s) 34, 271, 395, 1108
SexAI ACCWGGT 1 cut(s) 1092
SfaNI GCATC 2 cut(s) 70, 600
SfcI CTRYAG 2 cut(s) 603, 1453
SinI GGWCC 4 cut(s) 74, 444, 1555, 1713
SmiMI CAYNNNNRTG 2 cut(s) 165, 1649
SmlI CTYRAG 1 cut(s) 1708
SmoI CTYRAG 1 cut(s) 1708
SpeI ACTAGT 1 cut(s) 997
SsiI CCGC 5 cut(s) 312, 900, 981, 1588, 1619
SspMI CTAG 6 cut(s) 383, 938, 998, 1005, 1017, 1745
SstI GAGCTC 1 cut(s) 1108
StyD4I CCNGG 3 cut(s) 180, 690, 1092
StyI CCWWGG 2 cut(s) 1513, 1678
TaaI ACNGT 4 cut(s) 226, 497, 636, 1627
TaiI ACGT 5 cut(s) 342, 494, 616, 1026, 1446
TaqI TCGA 4 cut(s) 888, 905, 1195, 1704
TaqII GACCGA 1 cut(s) 62
TatI WGTACW 1 cut(s) 1030
TauI GCSGC 1 cut(s) 902
TfiI GAWTC 4 cut(s) 794, 916, 934, 1200
Tru1I TTAA 6 cut(s) 642, 713, 849, 861, 1440, 1709
Tru9I TTAA 6 cut(s) 642, 713, 849, 861, 1440, 1709
TscAI CASTG 6 cut(s) 564, 711, 1018, 1060, 1261, 1381
TseI GCWGC 3 cut(s) 59, 425, 1086
TspDTI ATGAA 8 cut(s) 90, 110, 226, 429, 737, 857, 874, 1686
TspGWI ACGGA 3 cut(s) 1098, 1543, 1721
TspRI CASTG 6 cut(s) 564, 711, 1018, 1060, 1261, 1381
Van91I CCANNNNNTGG 1 cut(s) 441
Vha464I CTTAAG 1 cut(s) 1708
VpaK11BI GGWCC 4 cut(s) 74, 444, 1555, 1713
XagI CCTNNNNNAGG 1 cut(s) 786
XapI RAATTY 3 cut(s) 97, 743, 907
XceI RCATGY 1 cut(s) 1507
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmiI GTMKAC 1 cut(s) 599
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 6 cut(s) 383, 938, 998, 1005, 1017, 1745
ZraI GACGTC 1 cut(s) 340
Zsp2I ATGCAT 1 cut(s) 1740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.