RchiOBHm_Chr5g0082941

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001157
Physical Location & Seq
Forward (+)
88736939 .. 88739349
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35711

Sequence Viewer

Length: 1860 bp
ATGACTGCACCAGTGCTAGCTCTGCCGGATTTTTCTAAACCATTTGTGGTAGAAACTGATGCAAGTGGTTTGGGTATAGGAGCGGTTCTATCCCAGCAACGCCATCCGATTGCATTCTTGAGCAAGTCTTTATCTCCTCGTAATCAGGCCTTGTCCGTATACGACAAGGAGATGCTCGCCGTTCTTTTTGCCATTGAGAAGTGGCGTCCATATCTGCTTGGTCATCCTTTCCAGATCCTCACTGACCATCAGACTCTCAAGCACTTGTTGAACCAGCGTATTTCAACACCCTCTCAACACAAATGGTTAGCCAAATTAATGGGCTACAATTACACCATTGAATATCGGGCTGGTAGTGCCAATACGGTGCCAGATCTGCTTTCTAGACGTCATGAGCTTTGCCCGCTTCAGGCGGTTTCTGCTCCGGTTTTCGATTGTTTACCCCAAATCGATCAGGCATGCCTCCGTGATCAGCACGCTCAAGTCATCATATCCCAATTACAACAAGGTCTTACGGCCAAGAAAGGTTTCAGTTGGAGCAATAATCGACTATTGTACAAAGGTAAAGTCTTTGTCCCTGTGTCCTCAGATTGGCGCACTAAGTTATTGTATGAGTTCCATTCTTCTCTACAAGCTGGGCATTCGGGGTACCTACGCACATATTTGCGTCTCTTTCGCAATTTTGCTTGGCCGGGAATGCGGAAAGAAATCAAATCCTTCATAGCAGAGTGTGATCAATGCCAGAGGCAAACTTATGAAACCATCAAACCACCAGGTCTCCTCCAACCTCTACCAATCCCGGAAAACATATGGTTTGATATCTCAATGGATTTTGTGGATGGTCTTCCCCCTTCTGATGGAAAAAATGCGATCTTTGTCGTAGTGGACCGCCTCTCTAAGTACGGGCATTTCATTGCAATCTCTCACCCCTATTCTGCTACTCAAATCGCAGAAGTGTTCATGAAAGAAGTATTTCGCCTTCATGGAATGCCCAAGTCGATTGTCAGTGATCGTGATCCTATCTTCATCAGCCATTTTTGGACAGCCTTCTTTAAGCTGCAAGGTACCACACTCTGCCGAAGCTCCGCATACCATCCGCAAACGGATGGACAAACCGAGGTTGTTAATCGCTCTTTGGAGCATTATTTGAGGTGTTTTGTGCTGGACAAACCCTCCTCTTGGCCTACACTGCTTCATTGGGCAGAATGGTGGTACAATTCTACTTACCATTCCACCATTCGAATGACTCCGTTTCAAGCAGTTTATGGTATTCCCCCTCCCTCTGTTGCTATGTATGTACCGGGCTCTACTTCAGTTCAGAAGGTGGACAAGGCACTCCAAGACCGCAATGTCCTTGTCCAATCACTCAAGATTCACATGGCAGCAACACAGAACCGAATGAAGCAGTCTGCTGATCAGCACCGCACGGAAAGAGTGTTCAACATTGGCGAATGGGTGTTTCTGAAATTACACCCCTACCGGCAACAATCGTTGGTGAAACGCGCAGTACACAAGCTATCTCCACGCTACTATGGCCCCTTCTTAATCCTAGAACGCATTGGCAAAGTGGCCTACAAGCTCGAGCTGCCTCCTCAATGCCGCATCCACCCCATTTTTCATGTCTCCCTTCTGAAGCTGCGTATCGGAGATGGTACGCCCTTTACTACTTCCCTCCCTGAATTTGATGACCAAGGTGACTTATTATGGCGTCCTGAAAAAGTCCTAGACTTGGCTGTGGTGCGTAAGAAGAAACGCAATATCACTCAATGGCTGATACAGTGGGCGGGTCTCCCTGCTGAAGATGCTACGTGGGAAGACGCTCACACCATCAAACATCGTTTCCCAGAATTTTGCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

619

Amino Acids

71.19

Weight (kDa)

9.39

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 2 - 79 5.7e-26 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 10 - 109 3.3e-32 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 196 - 250 8e-17 Integrase zinc binding domain
rve PF00665 275 - 366 7.7e-07 Integrase core domain
SH3_Tf2-1 PF24626 483 - 545 1.1e-22 Tf2-1-like, SH3 domain
Chromo PF00385 570 - 616 2.5e-07 Chromo (CHRromatin Organisation MOdifier) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1349
AatII GACGTC 1 cut(s) 391
Acc65I GGTACC 2 cut(s) 648, 1064
AccB1I GGYRCC 3 cut(s) 367, 648, 1064
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 159
AccII CGCG 1 cut(s) 1503
AclWI GGATC 2 cut(s) 229, 1010
AcoI YGGCCR 2 cut(s) 516, 689
AcsI RAATTY 2 cut(s) 1679, 1847
AcuI CTGAAG 4 cut(s) 392, 1296, 1652, 1818
AcyI GRCGYC 3 cut(s) 205, 388, 1708
AfaI GTAC 8 cut(s) 557, 650, 902, 1066, 1214, 1299, 1509, 1654
AfiI CCNNNNNNNGG 5 cut(s) 409, 591, 857, 1179, 1729
AgsI TTSAA 5 cut(s) 271, 285, 341, 1256, 1441
AjnI CCWGG 1 cut(s) 772
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AloI GAACNNNNNNTCC 2 cut(s) 1421, 1453
AluBI AGCT 9 cut(s) 20, 397, 635, 1057, 1083, 1516, 1579, 1585, 1636
AluI AGCT 9 cut(s) 20, 397, 635, 1057, 1083, 1516, 1579, 1585, 1636
Alw26I GTCTC 4 cut(s) 674, 782, 1627, 1793
AlwI GGATC 2 cut(s) 229, 1010
Ama87I CYCGRG 1 cut(s) 1580
AoxI GGCC 6 cut(s) 147, 516, 689, 1181, 1534, 1569
ApeKI GCWGC 4 cut(s) 1057, 1382, 1585, 1636
ApoI RAATTY 2 cut(s) 1679, 1847
ArsI GACNNNNNNTTYG 2 cut(s) 552, 584
AseI ATTAAT 1 cut(s) 317
Asp700I GAANNNNTTC 2 cut(s) 527, 972
Asp718I GGTACC 2 cut(s) 648, 1064
AspLEI GCGC 3 cut(s) 597, 1505, 1856
AspS9I GGNCC 2 cut(s) 886, 1535
AsuC2I CCSGG 3 cut(s) 693, 800, 1302
AsuHPI GGTGA 3 cut(s) 917, 1507, 1706
AsuII TTCGAA 1 cut(s) 1240
AsuNHI GCTAGC 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 1580
AvaII GGWCC 1 cut(s) 886
BanI GGYRCC 3 cut(s) 367, 648, 1064
BanII GRGCYC 1 cut(s) 1307
BarI GAAGNNNNNNTAC 2 cut(s) 963, 995
BbsI GAAGAC 2 cut(s) 836, 1821
BbvI GCAGC 4 cut(s) 1044, 1394, 1572, 1623
BccI CCATC 9 cut(s) 111, 255, 770, 833, 851, 1100, 1101, 1643, 1835
BceAI ACGGC 2 cut(s) 164, 531
BciT130I CCWGG 1 cut(s) 774
BclI TGATCA 3 cut(s) 469, 733, 1414
BcnI CCSGG 3 cut(s) 693, 800, 1302
BcoDI GTCTC 4 cut(s) 674, 782, 1627, 1793
BfaI CTAG 4 cut(s) 17, 384, 1550, 1724
BglII AGATCT 1 cut(s) 373
BisI GCNGC 5 cut(s) 1058, 1383, 1586, 1600, 1637
BlsI GCNGC 5 cut(s) 1059, 1384, 1587, 1601, 1638
Bme1390I CCNGG 4 cut(s) 693, 774, 800, 1302
Bme18I GGWCC 1 cut(s) 886
BmeT110I CYCGRG 1 cut(s) 1580
BmgT120I GGNCC 2 cut(s) 886, 1535
BmiI GGNNCC 4 cut(s) 369, 650, 1066, 1537
BmrFI CCNGG 4 cut(s) 693, 774, 800, 1302
BmsI GCATC 4 cut(s) 49, 162, 1611, 1792
BmtI GCTAGC 1 cut(s) 20
BpiI GAAGAC 2 cut(s) 836, 1821
Bpu14I TTCGAA 1 cut(s) 1240
BpuEI CTTGAG 4 cut(s) 139, 242, 465, 1352
BpuMI CCSGG 3 cut(s) 693, 800, 1302
Bsa29I ATCGAT 1 cut(s) 450
BsaAI YACGTR 1 cut(s) 1809
BsaBI GATNNNNATC 1 cut(s) 1014
BsaHI GRCGYC 3 cut(s) 205, 388, 1708
BsaI GGTCTC 2 cut(s) 782, 1793
BsaJI CCNNGG 2 cut(s) 1116, 1690
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 4 cut(s) 762, 792, 1157, 1187
Bsc4I CCNNNNNNNGG 5 cut(s) 409, 591, 857, 1179, 1729
Bse118I RCCGGY 1 cut(s) 1479
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 2 cut(s) 912, 1354
Bse8I GATNNNNATC 1 cut(s) 1014
BseBI CCWGG 1 cut(s) 774
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 2 cut(s) 1116, 1690
BseGI GGATG 6 cut(s) 103, 223, 844, 1093, 1111, 1602
BseJI GATNNNNATC 1 cut(s) 1014
BseLI CCNNNNNNNGG 5 cut(s) 409, 591, 857, 1179, 1729
BseMI GCAATG 2 cut(s) 912, 1354
BseMII CTCAG 1 cut(s) 600
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 4 cut(s) 126, 770, 1165, 1581
BseXI GCAGC 4 cut(s) 1044, 1394, 1572, 1623
BseYI CCCAGC 2 cut(s) 93, 635
Bsh1236I CGCG 1 cut(s) 1503
BshFI GGCC 6 cut(s) 149, 518, 691, 1183, 1536, 1571
BshNI GGYRCC 3 cut(s) 367, 648, 1064
BshVI ATCGAT 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 1580
BsiSI CCGG 6 cut(s) 26, 425, 692, 800, 1301, 1480
BslFI GGGAC 1 cut(s) 560
BslI CCNNNNNNNGG 5 cut(s) 409, 591, 857, 1179, 1729
BsmAI GTCTC 4 cut(s) 674, 782, 1627, 1793
BsmBI CGTCTC 1 cut(s) 674
BsmFI GGGAC 1 cut(s) 560
BsmI GAATGC 4 cut(s) 113, 640, 702, 993
BsnI GGCC 6 cut(s) 149, 518, 691, 1183, 1536, 1571
Bso31I GGTCTC 2 cut(s) 782, 1793
BsoBI CYCGRG 1 cut(s) 1580
Bsp119I TTCGAA 1 cut(s) 1240
Bsp1286I GDGCHC 1 cut(s) 1307
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 9 cut(s) 234, 373, 451, 469, 733, 870, 1009, 1015, 1414
BspANI GGCC 6 cut(s) 149, 518, 691, 1183, 1536, 1571
BspCNI CTCAG 1 cut(s) 599
BspDI ATCGAT 1 cut(s) 450
BspFNI CGCG 1 cut(s) 1503
BspHI TCATGA 2 cut(s) 391, 960
BspLI GGNNCC 4 cut(s) 369, 650, 1066, 1537
BspOI GCTAGC 1 cut(s) 20
BspPI GGATC 2 cut(s) 229, 1010
BspT104I TTCGAA 1 cut(s) 1240
BspT107I GGYRCC 3 cut(s) 367, 648, 1064
BspTNI GGTCTC 2 cut(s) 782, 1793
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 912, 1354
BsrFI RCCGGY 1 cut(s) 1479
BsrGI TGTACA 1 cut(s) 555
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 1479
BssECI CCNNGG 2 cut(s) 1116, 1690
BssMI GATC 9 cut(s) 234, 373, 451, 469, 733, 870, 1009, 1015, 1414
BssNAI GTATAC 1 cut(s) 160
BssNI GRCGYC 3 cut(s) 205, 388, 1708
BssT1I CCWWGG 1 cut(s) 1690
Bst1107I GTATAC 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 2 cut(s) 367, 1779
BstACI GRCGYC 3 cut(s) 205, 388, 1708
BstAUI TGTACA 1 cut(s) 555
BstBAI YACGTR 1 cut(s) 1809
BstBI TTCGAA 1 cut(s) 1240
BstC8I GCNNGC 5 cut(s) 18, 177, 404, 460, 477
BstDEI CTNAG 3 cut(s) 586, 600, 897
BstF5I GGATG 6 cut(s) 103, 223, 844, 1093, 1111, 1602
BstFNI CGCG 1 cut(s) 1503
BstHHI GCGC 3 cut(s) 597, 1505, 1856
BstKTI GATC 9 cut(s) 237, 376, 454, 472, 736, 873, 1012, 1018, 1417
BstMAI GTCTC 4 cut(s) 674, 782, 1627, 1793
BstMBI GATC 9 cut(s) 234, 373, 451, 469, 733, 870, 1009, 1015, 1414
BstNI CCWGG 1 cut(s) 774
BstNSI RCATGY 1 cut(s) 462
BstSCI CCNGG 4 cut(s) 691, 772, 798, 1300
BstUI CGCG 1 cut(s) 1503
BstV1I GCAGC 4 cut(s) 1044, 1394, 1572, 1623
BstV2I GAAGAC 2 cut(s) 836, 1821
BstX2I RGATCY 2 cut(s) 234, 373
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 2 cut(s) 234, 373
BstZ17I GTATAC 1 cut(s) 160
Bsu15I ATCGAT 1 cut(s) 450
BsuRI GGCC 6 cut(s) 149, 518, 691, 1183, 1536, 1571
BsuTUI ATCGAT 1 cut(s) 450
BtsCI GGATG 6 cut(s) 103, 223, 844, 1093, 1111, 1602
BtsI GCAGTG 1 cut(s) 1187
BtsIMutI CAGTG 5 cut(s) 18, 240, 1012, 1187, 1784
Cac8I GCNNGC 5 cut(s) 18, 177, 404, 460, 477
CciI TCATGA 2 cut(s) 391, 960
CfoI GCGC 3 cut(s) 597, 1505, 1856
Cfr10I RCCGGY 1 cut(s) 1479
Cfr13I GGNCC 2 cut(s) 886, 1535
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 4 cut(s) 194, 656, 1697, 1826
CsiI ACCWGGT 1 cut(s) 772
Csp6I GTAC 8 cut(s) 556, 649, 901, 1065, 1213, 1298, 1508, 1653
CviAII CATG 6 cut(s) 392, 459, 961, 983, 1378, 1619
CviQI GTAC 8 cut(s) 556, 649, 901, 1065, 1213, 1298, 1508, 1653
DdeI CTNAG 3 cut(s) 586, 600, 897
DpnI GATC 9 cut(s) 236, 375, 453, 471, 735, 872, 1011, 1017, 1416
DpnII GATC 9 cut(s) 234, 373, 451, 469, 733, 870, 1009, 1015, 1414
DrdI GACNNNNNNGTC 1 cut(s) 1349
DseDI GACNNNNNNGTC 1 cut(s) 1349
EaeI YGGCCR 2 cut(s) 516, 689
Eco130I CCWWGG 1 cut(s) 1690
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 1307
Eco31I GGTCTC 2 cut(s) 782, 1793
Eco32I GATATC 1 cut(s) 820
Eco47I GGWCC 1 cut(s) 886
Eco57I CTGAAG 4 cut(s) 392, 1296, 1652, 1818
Eco88I CYCGRG 1 cut(s) 1580
EcoRII CCWGG 1 cut(s) 772
EcoRV GATATC 1 cut(s) 820
EcoT14I CCWWGG 1 cut(s) 1690
EcoT38I GRGCYC 1 cut(s) 1307
ErhI CCWWGG 1 cut(s) 1690
Esp3I CGTCTC 1 cut(s) 674
FaeI CATG 6 cut(s) 395, 462, 964, 986, 1381, 1622
FaqI GGGAC 1 cut(s) 560
FatI CATG 6 cut(s) 391, 458, 960, 982, 1377, 1618
FauI CCCGC 2 cut(s) 411, 1777
FauNDI CATATG 1 cut(s) 809
FbaI TGATCA 3 cut(s) 469, 733, 1414
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 5 cut(s) 1058, 1383, 1586, 1600, 1637
FokI GGATG 6 cut(s) 90, 210, 851, 1080, 1118, 1589
FriOI GRGCYC 1 cut(s) 1307
Fsp4HI GCNGC 5 cut(s) 1058, 1383, 1586, 1600, 1637
FspBI CTAG 4 cut(s) 17, 384, 1550, 1724
GlaI GCGC 3 cut(s) 596, 1504, 1855
GluI GCNGC 5 cut(s) 1058, 1383, 1586, 1600, 1637
GsaI CCCAGC 2 cut(s) 97, 639
HaeIII GGCC 6 cut(s) 149, 518, 691, 1183, 1536, 1571
HapII CCGG 6 cut(s) 26, 425, 692, 800, 1301, 1480
HgaI GACGC 4 cut(s) 194, 656, 1697, 1826
HhaI GCGC 3 cut(s) 597, 1505, 1856
Hin1I GRCGYC 3 cut(s) 205, 388, 1708
Hin1II CATG 6 cut(s) 395, 462, 964, 986, 1381, 1622
Hin6I GCGC 3 cut(s) 595, 1503, 1854
HinP1I GCGC 3 cut(s) 595, 1503, 1854
HinfI GANTC 3 cut(s) 253, 1246, 1372
HpaII CCGG 6 cut(s) 26, 425, 692, 800, 1301, 1480
HphI GGTGA 3 cut(s) 917, 1507, 1706
Hpy166II GTNNAC 5 cut(s) 160, 440, 886, 1327, 1510
Hpy188I TCNGA 8 cut(s) 108, 252, 589, 856, 1320, 1464, 1632, 1646
Hpy188III TCNNGA 8 cut(s) 118, 232, 384, 392, 961, 1013, 1369, 1712
Hpy8I GTNNAC 5 cut(s) 160, 440, 886, 1327, 1510
HpyAV CCTTC 7 cut(s) 727, 861, 989, 1057, 1315, 1549, 1637
HpyCH4III ACNGT 2 cut(s) 367, 1779
HpyCH4IV ACGT 2 cut(s) 388, 1808
HpyCH4V TGCA 5 cut(s) 8, 62, 113, 917, 1060
HpyF3I CTNAG 3 cut(s) 586, 600, 897
HpySE526I ACGT 2 cut(s) 388, 1808
Hsp92I GRCGYC 3 cut(s) 205, 388, 1708
Hsp92II CATG 6 cut(s) 395, 462, 964, 986, 1381, 1622
HspAI GCGC 3 cut(s) 595, 1503, 1854
KpnI GGTACC 2 cut(s) 652, 1068
Ksp22I TGATCA 3 cut(s) 469, 733, 1414
Kzo9I GATC 9 cut(s) 234, 373, 451, 469, 733, 870, 1009, 1015, 1414
LmnI GCTCC 5 cut(s) 80, 427, 537, 1088, 1138
Lsp1109I GCAGC 4 cut(s) 1044, 1394, 1572, 1623
LweI GCATC 4 cut(s) 49, 162, 1611, 1792
MabI ACCWGGT 1 cut(s) 772
MaeI CTAG 4 cut(s) 17, 384, 1550, 1724
MaeII ACGT 2 cut(s) 388, 1808
MaeIII GTNAC 1 cut(s) 1694
MalI GATC 9 cut(s) 236, 375, 453, 471, 735, 872, 1011, 1017, 1416
MbiI CCGCTC 1 cut(s) 83
MboI GATC 9 cut(s) 234, 373, 451, 469, 733, 870, 1009, 1015, 1414
MboII GAAGA 6 cut(s) 615, 836, 1015, 1759, 1811, 1826
MflI RGATCY 2 cut(s) 234, 373
MhlI GDGCHC 1 cut(s) 1307
MluCI AATT 8 cut(s) 314, 328, 497, 679, 1216, 1466, 1679, 1847
MlyI GAGTC 2 cut(s) 247, 1240
MmeI TCCRAC 2 cut(s) 515, 808
MroXI GAANNNNTTC 2 cut(s) 527, 972
MseI TTAA 4 cut(s) 317, 1053, 1125, 1544
MslI CAYNNNNRTG 1 cut(s) 1241
MspI CCGG 6 cut(s) 26, 425, 692, 800, 1301, 1480
MspR9I CCNGG 4 cut(s) 693, 774, 800, 1302
Mva1269I GAATGC 4 cut(s) 113, 640, 702, 993
MvaI CCWGG 1 cut(s) 774
MvnI CGCG 1 cut(s) 1503
NciI CCSGG 3 cut(s) 693, 800, 1302
NdeI CATATG 1 cut(s) 809
NdeII GATC 9 cut(s) 234, 373, 451, 469, 733, 870, 1009, 1015, 1414
NheI GCTAGC 1 cut(s) 16
NlaIII CATG 6 cut(s) 395, 462, 964, 986, 1381, 1622
NlaIV GGNNCC 4 cut(s) 369, 650, 1066, 1537
NmuCI GTSAC 1 cut(s) 1694
NspI RCATGY 1 cut(s) 462
NspV TTCGAA 1 cut(s) 1240
PaeI GCATGC 1 cut(s) 462
PaeR7I CTCGAG 1 cut(s) 1580
PagI TCATGA 2 cut(s) 391, 960
PceI AGGCCT 1 cut(s) 149
PctI GAATGC 4 cut(s) 113, 640, 702, 993
PdmI GAANNNNTTC 2 cut(s) 527, 972
PfeI GAWTC 1 cut(s) 1372
PfoI TCCNGGA 1 cut(s) 798
PkrI GCNGC 5 cut(s) 1059, 1384, 1587, 1601, 1638
PleI GAGTC 2 cut(s) 247, 1240
PpsI GAGTC 2 cut(s) 247, 1240
Ppu21I YACGTR 1 cut(s) 1809
PshBI ATTAAT 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 772
PspFI CCCAGC 2 cut(s) 93, 635
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 4 cut(s) 369, 650, 1066, 1537
PspPI GGNCC 2 cut(s) 886, 1535
PspXI VCTCGAGB 1 cut(s) 1580
PsuI RGATCY 2 cut(s) 234, 373
RsaI GTAC 8 cut(s) 557, 650, 902, 1066, 1214, 1299, 1509, 1654
RsaNI GTAC 8 cut(s) 556, 649, 901, 1065, 1213, 1298, 1508, 1653
RseI CAYNNNNRTG 1 cut(s) 1241
SaqAI TTAA 4 cut(s) 317, 1053, 1125, 1544
SatI GCNGC 5 cut(s) 1058, 1383, 1586, 1600, 1637
Sau3AI GATC 9 cut(s) 234, 373, 451, 469, 733, 870, 1009, 1015, 1414
Sau96I GGNCC 2 cut(s) 886, 1535
SchI GAGTC 2 cut(s) 247, 1240
ScrFI CCNGG 4 cut(s) 693, 774, 800, 1302
SduI GDGCHC 1 cut(s) 1307
SexAI ACCWGGT 1 cut(s) 772
SfaNI GCATC 4 cut(s) 49, 162, 1611, 1792
Sfr274I CTCGAG 1 cut(s) 1580
SfuI TTCGAA 1 cut(s) 1240
SinI GGWCC 1 cut(s) 886
SlaI CTCGAG 1 cut(s) 1580
SmiMI CAYNNNNRTG 1 cut(s) 1241
SmlI CTYRAG 5 cut(s) 118, 257, 480, 1367, 1580
SmoI CTYRAG 5 cut(s) 118, 257, 480, 1367, 1580
SphI GCATGC 1 cut(s) 462
Sse9I AATT 8 cut(s) 314, 328, 497, 679, 1216, 1466, 1679, 1847
SseBI AGGCCT 1 cut(s) 149
SspMI CTAG 4 cut(s) 17, 384, 1550, 1724
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 4 cut(s) 691, 772, 798, 1300
StyI CCWWGG 1 cut(s) 1690
TaaI ACNGT 2 cut(s) 367, 1779
TaiI ACGT 2 cut(s) 391, 1811
TaqI TCGA 6 cut(s) 432, 450, 547, 998, 1240, 1581
TasI AATT 8 cut(s) 314, 328, 497, 679, 1216, 1466, 1679, 1847
TatI WGTACW 2 cut(s) 555, 1507
TauI GCSGC 1 cut(s) 1602
TfiI GAWTC 1 cut(s) 1372
Tru1I TTAA 4 cut(s) 317, 1053, 1125, 1544
Tru9I TTAA 4 cut(s) 317, 1053, 1125, 1544
TscAI CASTG 5 cut(s) 18, 247, 1012, 1194, 1784
TseFI GTSAC 1 cut(s) 1694
TseI GCWGC 4 cut(s) 1057, 1382, 1585, 1636
Tsp45I GTSAC 1 cut(s) 1694
TspGWI ACGGA 5 cut(s) 145, 455, 1118, 1239, 1442
TspRI CASTG 5 cut(s) 18, 247, 1012, 1194, 1784
VpaK11BI GGWCC 1 cut(s) 886
VspI ATTAAT 1 cut(s) 317
XapI RAATTY 2 cut(s) 1679, 1847
XbaI TCTAGA 1 cut(s) 383
XceI RCATGY 1 cut(s) 462
XhoI CTCGAG 1 cut(s) 1580
XmiI GTMKAC 1 cut(s) 159
XmnI GAANNNNTTC 2 cut(s) 527, 972
XspI CTAG 4 cut(s) 17, 384, 1550, 1724
ZraI GACGTC 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.