RchiOBHm_Chr5g0056121

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001157
Physical Location & Seq
Forward (+)
59522387 .. 59523287
901 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33305

Sequence Viewer

Length: 858 bp
ATGTTCTTCTTTGTTGATGGTAACAACTATTGTTTGCAGGGCATCACCTCTCAAGACAACTACTCTGAGAAACCTGATTTGTTAGCTTTACTCAATCCAGAACAACTAGACTCTCTGCCAGGTTGGTCACCTCCACCCTTAACAGACCAATCAACAAAACCACCTTCCGGTGTCAAAACTCTCCTCTGTGCTTTCAGTGATTTGTTTGCACCACCGTCTGGGCTTCCACCACCTAGGCCCATAGACCACAAGATCAGTTTACTTCCCCACAGTGGGCCTGTGAACGTACGGCCTTACCGTTACCCCCATTCACAAAAGGCAGAGCTCGAGGCCCAGGTGAAAGAAATGCTTGCTACCGGTATTATTCGACCTAGTTCAAGCCCGTACTCTTCTCCGGTTCTATTAGTCAAGAAAAAGGAGGGTACATGGCGTTTTTGCGTCGACTATAGGTCCCTTAATGCTATTACCATTAAGGATAAATTCCCTATACCCATCGTCGACGAGTTGTTAGATGAATTACATGGAGCTAAGTATTTTTCCAAACTTGACCTTCGATCCGATTACCACCAAATCCGGATGAACACCAATGACATACCGAAAACCGCTTTTCGCACACATGATGGGCATTATGAGTTTGTGGTGATGCCTTTCGGTCTCACCAATGCGCCTTCAACGTTCCAATCCCTCATGAACCATATCTTCAAACCGGTACTTCGTAAGTTTGTCCTTGTTTTTTTTTATGACATTTTAATTTATAGTCCGGATTTGGATTCTCATATTGAACATTTAGAGGCTGTTTTTACTCTGTTGAGGGCTAATGATCTTAAAGTGAAAATGTCCAAGTGTGCTTTCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

285

Amino Acids

32.32

Weight (kDa)

7.14

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 136 - 285 1.7e-33 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 218
AccI GTMKAC 2 cut(s) 441, 498
AccIII TCCGGA 2 cut(s) 573, 760
AciI CCGC 1 cut(s) 603
AclI AACGTT 1 cut(s) 674
AclWI GGATC 1 cut(s) 549
AcsI RAATTY 1 cut(s) 479
AfaI GTAC 4 cut(s) 288, 386, 424, 711
AfiI CCNNNNNNNGG 4 cut(s) 167, 218, 272, 273
AgeI ACCGGT 2 cut(s) 356, 706
AgsI TTSAA 4 cut(s) 378, 672, 703, 782
AhdI GACNNNNNGTC 1 cut(s) 448
AjnI CCWGG 2 cut(s) 118, 333
AjuI GAANNNNNNNTTGG 1 cut(s) 833
AluBI AGCT 3 cut(s) 86, 325, 527
AluI AGCT 3 cut(s) 86, 325, 527
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 659
AlwI GGATC 1 cut(s) 549
Ama87I CYCGRG 1 cut(s) 326
Aor13HI TCCGGA 2 cut(s) 573, 760
AoxI GGCC 4 cut(s) 236, 275, 290, 330
ApoI RAATTY 1 cut(s) 479
AsiGI ACCGGT 2 cut(s) 356, 706
AspA2I CCTAGG 1 cut(s) 233
AspLEI GCGC 1 cut(s) 667
AspS9I GGNCC 4 cut(s) 237, 275, 331, 450
AsuHPI GGTGA 5 cut(s) 37, 120, 349, 649, 652
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 1 cut(s) 450
AvrII CCTAGG 1 cut(s) 233
BanII GRGCYC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 327
BccI CCATC 3 cut(s) 11, 500, 614
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 2 cut(s) 120, 335
BcoDI GTCTC 1 cut(s) 659
BfaI CTAG 4 cut(s) 107, 234, 372, 856
BfmI CTRYAG 1 cut(s) 445
BlnI CCTAGG 1 cut(s) 233
Bme1390I CCNGG 2 cut(s) 120, 335
Bme18I GGWCC 1 cut(s) 450
BmeRI GACNNNNNGTC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 4 cut(s) 237, 275, 331, 450
BmiI GGNNCC 1 cut(s) 452
BmrFI CCNGG 2 cut(s) 120, 335
BmsI GCATC 2 cut(s) 51, 633
BpuEI CTTGAG 1 cut(s) 36
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 2 cut(s) 233, 333
BsaWI WCCGGW 6 cut(s) 167, 356, 394, 573, 706, 760
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 218, 272, 273
Bse118I RCCGGY 2 cut(s) 356, 706
BseAI TCCGGA 2 cut(s) 573, 760
BseBI CCWGG 2 cut(s) 120, 335
BseDI CCNNGG 2 cut(s) 233, 333
BseGI GGATG 1 cut(s) 582
BseLI CCNNNNNNNGG 4 cut(s) 167, 218, 272, 273
BseMII CTCAG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 173
BshFI GGCC 4 cut(s) 238, 277, 292, 332
BshTI ACCGGT 2 cut(s) 356, 706
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 6 cut(s) 168, 357, 395, 574, 707, 761
BsiWI CGTACG 1 cut(s) 286
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 4 cut(s) 167, 218, 272, 273
BsmAI GTCTC 1 cut(s) 659
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 4 cut(s) 238, 277, 292, 332
Bso31I GGTCTC 1 cut(s) 659
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 327
Bsp13I TCCGGA 2 cut(s) 573, 760
Bsp143I GATC 3 cut(s) 252, 554, 820
BspACI CCGC 1 cut(s) 603
BspANI GGCC 4 cut(s) 238, 277, 292, 332
BspCNI CTCAG 1 cut(s) 58
BspEI TCCGGA 2 cut(s) 573, 760
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 452
BspPI GGATC 1 cut(s) 549
BspTNI GGTCTC 1 cut(s) 659
BsrFI RCCGGY 2 cut(s) 356, 706
BssAI RCCGGY 2 cut(s) 356, 706
BssECI CCNNGG 2 cut(s) 233, 333
BssMI GATC 3 cut(s) 252, 554, 820
BssT1I CCWWGG 1 cut(s) 233
Bst2UI CCWGG 2 cut(s) 120, 335
Bst4CI ACNGT 3 cut(s) 216, 272, 299
Bst6I CTCTTC 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 2 cut(s) 66, 528
BstEII GGTNACC 1 cut(s) 126
BstF5I GGATG 1 cut(s) 582
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 3 cut(s) 255, 557, 823
BstMAI GTCTC 1 cut(s) 659
BstMBI GATC 3 cut(s) 252, 554, 820
BstNI CCWGG 2 cut(s) 120, 335
BstPI GGTNACC 1 cut(s) 126
BstSCI CCNGG 2 cut(s) 118, 333
BstSFI CTRYAG 1 cut(s) 445
BsuRI GGCC 4 cut(s) 238, 277, 292, 332
BtsCI GGATG 1 cut(s) 582
BtsIMutI CAGTG 2 cut(s) 202, 277
Cac8I GCNNGC 1 cut(s) 351
CciI TCATGA 1 cut(s) 687
CfoI GCGC 1 cut(s) 667
Cfr10I RCCGGY 2 cut(s) 356, 706
Cfr13I GGNCC 4 cut(s) 237, 275, 331, 450
CseI GACGC 1 cut(s) 427
Csp6I GTAC 4 cut(s) 287, 385, 423, 710
CspAI ACCGGT 2 cut(s) 356, 706
CviAII CATG 4 cut(s) 426, 521, 617, 688
CviQI GTAC 4 cut(s) 287, 385, 423, 710
DdeI CTNAG 2 cut(s) 66, 528
DpnI GATC 3 cut(s) 254, 556, 822
DpnII GATC 3 cut(s) 252, 554, 820
DriI GACNNNNNGTC 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 394
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 394
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 325
Eco88I CYCGRG 1 cut(s) 326
Eco91I GGTNACC 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 450
EcoO65I GGTNACC 1 cut(s) 126
EcoRII CCWGG 2 cut(s) 118, 333
EcoT14I CCWWGG 1 cut(s) 233
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 233
FaeI CATG 4 cut(s) 429, 524, 620, 691
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FaqI GGGAC 1 cut(s) 436
FatI CATG 4 cut(s) 425, 520, 616, 687
FblI GTMKAC 2 cut(s) 441, 498
FokI GGATG 1 cut(s) 589
FriOI GRGCYC 1 cut(s) 327
FspBI CTAG 4 cut(s) 107, 234, 372, 856
GlaI GCGC 1 cut(s) 666
HaeIII GGCC 4 cut(s) 238, 277, 292, 332
HapII CCGG 6 cut(s) 168, 357, 395, 574, 707, 761
HgaI GACGC 1 cut(s) 427
HhaI GCGC 1 cut(s) 667
Hin1II CATG 4 cut(s) 429, 524, 620, 691
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HincII GTYRAC 2 cut(s) 442, 499
HindII GTYRAC 2 cut(s) 442, 499
HinfI GANTC 2 cut(s) 110, 770
HpaII CCGG 6 cut(s) 168, 357, 395, 574, 707, 761
HphI GGTGA 5 cut(s) 37, 120, 349, 649, 652
Hpy166II GTNNAC 4 cut(s) 260, 283, 442, 499
Hpy188I TCNGA 2 cut(s) 67, 559
Hpy188III TCNNGA 6 cut(s) 53, 98, 409, 574, 688, 761
Hpy8I GTNNAC 4 cut(s) 260, 283, 442, 499
Hpy99I CGWCG 3 cut(s) 443, 500, 503
HpyAV CCTTC 3 cut(s) 174, 560, 678
HpyCH4III ACNGT 3 cut(s) 216, 272, 299
HpyCH4IV ACGT 2 cut(s) 285, 674
HpyCH4V TGCA 2 cut(s) 37, 209
HpyF3I CTNAG 2 cut(s) 66, 528
HpySE526I ACGT 2 cut(s) 285, 674
Hsp92II CATG 4 cut(s) 429, 524, 620, 691
HspAI GCGC 1 cut(s) 665
Kpn2I TCCGGA 2 cut(s) 573, 760
Kzo9I GATC 3 cut(s) 252, 554, 820
LmnI GCTCC 1 cut(s) 524
LweI GCATC 2 cut(s) 51, 633
MaeI CTAG 4 cut(s) 107, 234, 372, 856
MaeII ACGT 2 cut(s) 285, 674
MaeIII GTNAC 3 cut(s) 20, 126, 299
MalI GATC 3 cut(s) 254, 556, 822
MboI GATC 3 cut(s) 252, 554, 820
MboII GAAGA 2 cut(s) 381, 691
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 3 cut(s) 479, 515, 750
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 8 cut(s) 58, 141, 194, 322, 412, 695, 784, 804
MroI TCCGGA 2 cut(s) 573, 760
MseI TTAA 5 cut(s) 140, 456, 471, 749, 825
MspI CCGG 6 cut(s) 168, 357, 395, 574, 707, 761
MspR9I CCNGG 2 cut(s) 120, 335
MvaI CCWGG 2 cut(s) 120, 335
NdeII GATC 3 cut(s) 252, 554, 820
NlaIII CATG 4 cut(s) 429, 524, 620, 691
NlaIV GGNNCC 1 cut(s) 452
NmuCI GTSAC 1 cut(s) 126
PaeR7I CTCGAG 1 cut(s) 326
PagI TCATGA 1 cut(s) 687
PcsI WCGNNNNNNNCGW 1 cut(s) 295
PfeI GAWTC 1 cut(s) 770
Pfl23II CGTACG 1 cut(s) 286
PflMI CCANNNNNTGG 1 cut(s) 218
PinAI ACCGGT 2 cut(s) 356, 706
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PpuMI RGGWCCY 1 cut(s) 450
Psp124BI GAGCTC 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 674
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 2 cut(s) 118, 333
PspEI GGTNACC 1 cut(s) 126
PspGI CCWGG 2 cut(s) 118, 333
PspLI CGTACG 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 452
PspPI GGNCC 4 cut(s) 237, 275, 331, 450
PspPPI RGGWCCY 1 cut(s) 450
PspXI VCTCGAGB 1 cut(s) 326
RsaI GTAC 4 cut(s) 288, 386, 424, 711
RsaNI GTAC 4 cut(s) 287, 385, 423, 710
SacI GAGCTC 1 cut(s) 327
SalI GTCGAC 2 cut(s) 440, 497
SaqAI TTAA 5 cut(s) 140, 456, 471, 749, 825
Sau3AI GATC 3 cut(s) 252, 554, 820
Sau96I GGNCC 4 cut(s) 237, 275, 331, 450
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 120, 335
SduI GDGCHC 1 cut(s) 327
SfaNI GCATC 2 cut(s) 51, 633
SfcI CTRYAG 1 cut(s) 445
Sfr274I CTCGAG 1 cut(s) 326
SgrDI CGTCGACG 1 cut(s) 497
SinI GGWCC 1 cut(s) 450
SlaI CTCGAG 1 cut(s) 326
SmlI CTYRAG 2 cut(s) 51, 326
SmoI CTYRAG 2 cut(s) 51, 326
Sse9I AATT 3 cut(s) 479, 515, 750
SsiI CCGC 1 cut(s) 603
SspMI CTAG 4 cut(s) 107, 234, 372, 856
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 118, 333
StyI CCWWGG 1 cut(s) 233
TaaI ACNGT 3 cut(s) 216, 272, 299
TaiI ACGT 2 cut(s) 288, 677
TaqI TCGA 5 cut(s) 327, 367, 441, 498, 553
TaqII GACCGA 1 cut(s) 641
TasI AATT 3 cut(s) 479, 515, 750
TfiI GAWTC 1 cut(s) 770
Tru1I TTAA 5 cut(s) 140, 456, 471, 749, 825
Tru9I TTAA 5 cut(s) 140, 456, 471, 749, 825
TscAI CASTG 2 cut(s) 202, 277
TseFI GTSAC 1 cut(s) 126
Tsp45I GTSAC 1 cut(s) 126
TspDTI ATGAA 3 cut(s) 528, 593, 704
TspRI CASTG 2 cut(s) 202, 277
Van91I CCANNNNNTGG 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 1 cut(s) 479
XhoI CTCGAG 1 cut(s) 326
XmaJI CCTAGG 1 cut(s) 233
XmiI GTMKAC 2 cut(s) 441, 498
XspI CTAG 4 cut(s) 107, 234, 372, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.