Rroxscaffold_159G00432700

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000159
OG0001157
Physical Location & Seq
Reverse (-)
308054 .. 313032
4979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_159G00432700.1

Sequence Viewer

Length: 930 bp
ATGGCAGAGCGGTACATCCGAGCCCAACGCATCCCCCACCACGAGCAAGTTACATCCATTGCCTCACACTTTGGGCCCGACGCATCCATATGGATGAATTCATATGAGCAGCGCAACCCCACCATCTCTTGGGAGCAGTTCACAGCAGCTTTCTTGGAACACTTTGGCGGTGGAAATTCCACTGATTTCAAAACTGCCCTCTCCCACCTCCAACACACCACGTTGATTGACGAATGCATCCACACCTTCACCAAGCTGTCATGTCGAGCTCCTGATTGGTTTGATGACCAACTTCTTCCAATTTTCCTTGGTGGTTTGAAACCGGAGCTTAGACATGACGTCATTGCCTTGGAGCCCACCACCTTGGCCAAAGCTCAGCGTCTAGCTCGACGCTTTGAGGCATGTTTGCTTGACCTTAGAGCTCATCGGACATACCGTCCCACTCCATGGTCCCAGCAATCACTTACTGGCCCACACTCCGCCACATCCCACAACACATCTCACCAAATTGCCTCTGCCTCTAACTCTCATCTGACATCCGTCACCCACAACTCCACCATAAATAGCCAAACAAATCAGCGTACCAAACCGGAGGCCCCTTTTCACAAATGGACCATTGCAAAGCAACGCGAGCGTCGAGCACAAGGTTTGTGTTTCAATTGTGATGAACCTTGGTCCAAAACTCATACTTGCAAAAAGCCCATTTTGGCAATCTTGGAAGGCTCTTCGGTGGCCTCTGATGAATTTGACGCCGAGTCCCCACCGGAAGACCCAATTGAGCCTCCTGAGTTACCGCTCCACGCCATCTCCAACTCTAAAATCAGTGAGATGATGCGCCTCATGGGTTCGATCAACGACTTACCCATCTCTGTGTTCATCGATTGCGGCTCGGTGATGAACTTCTTGAATCCGGCCGTGGCCCACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

309

Amino Acids

34.75

Weight (kDa)

6.52

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 9 - 133 1.7e-12 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 21 - 108 2.1e-11 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 129, 447
AccBSI CCGCTC 2 cut(s) 10, 796
AccII CGCG 1 cut(s) 630
AciI CCGC 5 cut(s) 10, 168, 480, 794, 885
AcoI YGGCCR 2 cut(s) 366, 912
AcsI RAATTY 3 cut(s) 97, 175, 743
AcyI GRCGYC 2 cut(s) 339, 750
AfaI GTAC 2 cut(s) 14, 583
AfiI CCNNNNNNNGG 2 cut(s) 129, 447
AgsI TTSAA 4 cut(s) 190, 319, 658, 907
AhdI GACNNNNNGTC 3 cut(s) 338, 435, 754
AluBI AGCT 7 cut(s) 149, 256, 269, 328, 374, 386, 422
AluI AGCT 7 cut(s) 149, 256, 269, 328, 374, 386, 422
Alw21I GWGCWC 3 cut(s) 271, 424, 643
AoxI GGCC 7 cut(s) 74, 366, 469, 594, 732, 912, 918
ApaI GGGCCC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 109, 146
ApoI RAATTY 3 cut(s) 97, 175, 743
AspLEI GCGC 2 cut(s) 114, 837
AspS9I GGNCC 8 cut(s) 74, 75, 450, 470, 595, 612, 675, 919
AsuHPI GGTGA 4 cut(s) 241, 494, 535, 904
AvaII GGWCC 3 cut(s) 450, 612, 675
BaeGI GKGCMC 1 cut(s) 78
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 5 cut(s) 25, 78, 271, 357, 424
BauI CACGAG 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 774
Bbv12I GWGCWC 3 cut(s) 271, 424, 643
BbvI GCAGC 2 cut(s) 121, 158
BccI CCATC 3 cut(s) 131, 812, 872
BceAI ACGGC 1 cut(s) 899
BfaI CTAG 1 cut(s) 383
BisI GCNGC 3 cut(s) 110, 147, 886
BlpI GCTNAGC 1 cut(s) 375
BlsI GCNGC 3 cut(s) 111, 148, 887
Bme18I GGWCC 3 cut(s) 450, 612, 675
BmeRI GACNNNNNGTC 3 cut(s) 338, 435, 754
BmgT120I GGNCC 8 cut(s) 74, 75, 450, 470, 595, 612, 675, 919
BmiI GGNNCC 4 cut(s) 76, 354, 452, 597
BmsI GCATC 4 cut(s) 39, 92, 246, 822
BoxI GACNNNNGTC 1 cut(s) 539
BpiI GAAGAC 1 cut(s) 774
Bpu1102I GCTNAGC 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 879
BsaHI GRCGYC 2 cut(s) 339, 750
BsaJI CCNNGG 6 cut(s) 307, 348, 363, 446, 671, 915
BsaWI WCCGGW 3 cut(s) 322, 589, 763
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 447
Bse1I ACTGG 1 cut(s) 472
Bse3DI GCAATG 3 cut(s) 57, 342, 615
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 6 cut(s) 307, 348, 363, 446, 671, 915
BseGI GGATG 8 cut(s) 15, 30, 53, 83, 99, 237, 485, 536
BseLI CCNNNNNNNGG 2 cut(s) 129, 447
BseMI GCAATG 3 cut(s) 57, 342, 615
BseMII CTCAG 2 cut(s) 389, 777
BseNI ACTGG 1 cut(s) 472
BseSI GKGCMC 1 cut(s) 78
BseX3I CGGCCG 1 cut(s) 912
BseXI GCAGC 2 cut(s) 121, 158
BseYI CCCAGC 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 630
Bsh1285I CGRYCG 1 cut(s) 915
BshFI GGCC 7 cut(s) 76, 368, 471, 596, 734, 914, 920
BshVI ATCGAT 1 cut(s) 879
BsiEI CGRYCG 1 cut(s) 915
BsiHKAI GWGCWC 3 cut(s) 271, 424, 643
BsiSI CCGG 4 cut(s) 323, 590, 764, 911
BslFI GGGAC 3 cut(s) 423, 436, 742
BslI CCNNNNNNNGG 2 cut(s) 129, 447
BsmFI GGGAC 3 cut(s) 423, 436, 742
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 7 cut(s) 76, 368, 471, 596, 734, 914, 920
Bsp120I GGGCCC 1 cut(s) 74
Bsp1286I GDGCHC 6 cut(s) 25, 78, 271, 357, 424, 643
Bsp143I GATC 1 cut(s) 849
Bsp1720I GCTNAGC 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 446
BspACI CCGC 5 cut(s) 10, 168, 480, 794, 885
BspANI GGCC 7 cut(s) 76, 368, 471, 596, 734, 914, 920
BspCNI CTCAG 2 cut(s) 388, 778
BspDI ATCGAT 1 cut(s) 879
BspFNI CGCG 1 cut(s) 630
BspLI GGNNCC 4 cut(s) 76, 354, 452, 597
BspQI GCTCTTC 1 cut(s) 730
BsrBI CCGCTC 2 cut(s) 10, 796
BsrDI GCAATG 3 cut(s) 57, 342, 615
BsrI ACTGG 1 cut(s) 472
BssECI CCNNGG 6 cut(s) 307, 348, 363, 446, 671, 915
BssMI GATC 1 cut(s) 849
BssNI GRCGYC 2 cut(s) 339, 750
BssSI CACGAG 1 cut(s) 41
BssT1I CCWWGG 5 cut(s) 307, 348, 363, 446, 671
Bst2BI CACGAG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 437
Bst6I CTCTTC 1 cut(s) 730
BstACI GRCGYC 2 cut(s) 339, 750
BstC8I GCNNGC 1 cut(s) 632
BstDEI CTNAG 4 cut(s) 329, 375, 416, 786
BstDSI CCRYGG 2 cut(s) 446, 915
BstF5I GGATG 8 cut(s) 15, 30, 53, 83, 99, 237, 485, 536
BstFNI CGCG 1 cut(s) 630
BstHHI GCGC 2 cut(s) 114, 837
BstKTI GATC 1 cut(s) 852
BstMBI GATC 1 cut(s) 849
BstMCI CGRYCG 1 cut(s) 915
BstMWI GCNNNNNNNGC 1 cut(s) 631
BstNSI RCATGY 1 cut(s) 405
BstPAI GACNNNNGTC 1 cut(s) 539
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 2 cut(s) 121, 158
BstV2I GAAGAC 1 cut(s) 774
BstXI CCANNNNNNTGG 1 cut(s) 364
BstZI CGGCCG 1 cut(s) 912
Bsu15I ATCGAT 1 cut(s) 879
BsuRI GGCC 7 cut(s) 76, 368, 471, 596, 734, 914, 920
BsuTUI ATCGAT 1 cut(s) 879
BtgI CCRYGG 2 cut(s) 446, 915
BtsCI GGATG 8 cut(s) 15, 30, 53, 83, 99, 237, 485, 536
BtsIMutI CAGTG 2 cut(s) 180, 829
Cac8I GCNNGC 1 cut(s) 632
CfoI GCGC 2 cut(s) 114, 837
Cfr13I GGNCC 8 cut(s) 74, 75, 450, 470, 595, 612, 675, 919
ClaI ATCGAT 1 cut(s) 879
CseI GACGC 5 cut(s) 89, 368, 399, 623, 758
Csp6I GTAC 2 cut(s) 13, 582
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 5 cut(s) 261, 335, 402, 447, 841
CviQI GTAC 2 cut(s) 13, 582
DdeI CTNAG 4 cut(s) 329, 375, 416, 786
DpnI GATC 1 cut(s) 851
DpnII GATC 1 cut(s) 849
DriI GACNNNNNGTC 3 cut(s) 338, 435, 754
EaeI YGGCCR 2 cut(s) 366, 912
EagI CGGCCG 1 cut(s) 912
Eam1104I CTCTTC 1 cut(s) 730
Eam1105I GACNNNNNGTC 3 cut(s) 338, 435, 754
EarI CTCTTC 1 cut(s) 730
EciI GGCGGA 1 cut(s) 469
Ecl136II GAGCTC 2 cut(s) 269, 422
EclXI CGGCCG 1 cut(s) 912
Eco130I CCWWGG 5 cut(s) 307, 348, 363, 446, 671
Eco24I GRGCYC 5 cut(s) 25, 78, 271, 357, 424
Eco47I GGWCC 3 cut(s) 450, 612, 675
Eco52I CGGCCG 1 cut(s) 912
Eco53kI GAGCTC 2 cut(s) 269, 422
EcoICRI GAGCTC 2 cut(s) 269, 422
EcoO109I RGGNCCY 1 cut(s) 595
EcoRI GAATTC 1 cut(s) 97
EcoT14I CCWWGG 5 cut(s) 307, 348, 363, 446, 671
EcoT22I ATGCAT 1 cut(s) 239
EcoT38I GRGCYC 5 cut(s) 25, 78, 271, 357, 424
ErhI CCWWGG 5 cut(s) 307, 348, 363, 446, 671
FaeI CATG 5 cut(s) 264, 338, 405, 450, 844
FaqI GGGAC 3 cut(s) 423, 436, 742
FatI CATG 5 cut(s) 260, 334, 401, 446, 840
FauNDI CATATG 2 cut(s) 89, 103
Fnu4HI GCNGC 3 cut(s) 110, 147, 886
FokI GGATG 8 cut(s) 2, 17, 40, 70, 106, 224, 472, 523
FriOI GRGCYC 5 cut(s) 25, 78, 271, 357, 424
Fsp4HI GCNGC 3 cut(s) 110, 147, 886
FspBI CTAG 1 cut(s) 383
GlaI GCGC 2 cut(s) 113, 836
GluI GCNGC 3 cut(s) 110, 147, 886
GsaI CCCAGC 1 cut(s) 457
HaeIII GGCC 7 cut(s) 76, 368, 471, 596, 734, 914, 920
HapII CCGG 4 cut(s) 323, 590, 764, 911
HgaI GACGC 5 cut(s) 89, 368, 399, 623, 758
HhaI GCGC 2 cut(s) 114, 837
Hin1I GRCGYC 2 cut(s) 339, 750
Hin1II CATG 5 cut(s) 264, 338, 405, 450, 844
Hin6I GCGC 2 cut(s) 112, 835
HinP1I GCGC 2 cut(s) 112, 835
HinfI GANTC 2 cut(s) 755, 907
HpaII CCGG 4 cut(s) 323, 590, 764, 911
HphI GGTGA 4 cut(s) 241, 494, 535, 904
Hpy166II GTNNAC 1 cut(s) 141
Hpy188I TCNGA 4 cut(s) 20, 429, 534, 739
Hpy188III TCNNGA 3 cut(s) 272, 785, 904
Hpy8I GTNNAC 1 cut(s) 141
Hpy99I CGWCG 3 cut(s) 83, 393, 639
HpyAV CCTTC 2 cut(s) 256, 713
HpyCH4III ACNGT 1 cut(s) 437
HpyCH4IV ACGT 2 cut(s) 221, 339
HpyCH4V TGCA 3 cut(s) 237, 620, 693
HpyF10VI GCNNNNNNNGC 1 cut(s) 631
HpyF3I CTNAG 4 cut(s) 329, 375, 416, 786
HpySE526I ACGT 2 cut(s) 221, 339
Hsp92I GRCGYC 2 cut(s) 339, 750
Hsp92II CATG 5 cut(s) 264, 338, 405, 450, 844
HspAI GCGC 2 cut(s) 112, 835
Kzo9I GATC 1 cut(s) 849
LguI GCTCTTC 1 cut(s) 730
LmnI GCTCC 5 cut(s) 133, 274, 325, 352, 801
LpnPI CCDG 8 cut(s) 285, 336, 453, 467, 603, 777, 798, 924
Lsp1109I GCAGC 2 cut(s) 121, 158
LweI GCATC 4 cut(s) 39, 92, 246, 822
MaeI CTAG 1 cut(s) 383
MaeII ACGT 2 cut(s) 221, 339
MaeIII GTNAC 3 cut(s) 49, 541, 789
MalI GATC 1 cut(s) 851
MbiI CCGCTC 2 cut(s) 10, 796
MboI GATC 1 cut(s) 849
MboII GAAGA 3 cut(s) 287, 717, 779
MfeI CAATTG 2 cut(s) 658, 774
MhlI GDGCHC 6 cut(s) 25, 78, 271, 357, 424, 643
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 7 cut(s) 97, 175, 300, 507, 658, 743, 774
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 764
MmeI TCCRAC 2 cut(s) 235, 834
Mox20I TGGCCA 1 cut(s) 368
Mph1103I ATGCAT 1 cut(s) 239
MscI TGGCCA 1 cut(s) 368
MslI CAYNNNNRTG 3 cut(s) 88, 92, 869
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 4 cut(s) 323, 590, 764, 911
MunI CAATTG 2 cut(s) 658, 774
Mva1269I GAATGC 1 cut(s) 239
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 1 cut(s) 631
NcoI CCATGG 1 cut(s) 446
NdeI CATATG 2 cut(s) 89, 103
NdeII GATC 1 cut(s) 849
NlaIII CATG 5 cut(s) 264, 338, 405, 450, 844
NlaIV GGNNCC 4 cut(s) 76, 354, 452, 597
NmeAIII GCCGAG 1 cut(s) 778
NmuCI GTSAC 1 cut(s) 541
NsiI ATGCAT 1 cut(s) 239
NspI RCATGY 1 cut(s) 405
PciSI GCTCTTC 1 cut(s) 730
PcsI WCGNNNNNNNCGW 2 cut(s) 433, 634
PctI GAATGC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 907
PflMI CCANNNNNTGG 2 cut(s) 129, 447
PkrI GCNGC 3 cut(s) 111, 148, 887
PleI GAGTC 1 cut(s) 763
PpsI GAGTC 1 cut(s) 763
PshAI GACNNNNGTC 1 cut(s) 539
Psp124BI GAGCTC 2 cut(s) 271, 424
PspFI CCCAGC 1 cut(s) 453
PspN4I GGNNCC 4 cut(s) 76, 354, 452, 597
PspOMI GGGCCC 1 cut(s) 74
PspPI GGNCC 8 cut(s) 74, 75, 450, 470, 595, 612, 675, 919
RsaI GTAC 2 cut(s) 14, 583
RsaNI GTAC 2 cut(s) 13, 582
RseI CAYNNNNRTG 3 cut(s) 88, 92, 869
SacI GAGCTC 2 cut(s) 271, 424
SapI GCTCTTC 1 cut(s) 730
SatI GCNGC 3 cut(s) 110, 147, 886
Sau3AI GATC 1 cut(s) 849
Sau96I GGNCC 8 cut(s) 74, 75, 450, 470, 595, 612, 675, 919
SchI GAGTC 1 cut(s) 764
SduI GDGCHC 6 cut(s) 25, 78, 271, 357, 424, 643
SfaNI GCATC 4 cut(s) 39, 92, 246, 822
SinI GGWCC 3 cut(s) 450, 612, 675
SmiMI CAYNNNNRTG 3 cut(s) 88, 92, 869
Sse9I AATT 7 cut(s) 97, 175, 300, 507, 658, 743, 774
SsiI CCGC 5 cut(s) 10, 168, 480, 794, 885
SspMI CTAG 1 cut(s) 383
SstI GAGCTC 2 cut(s) 271, 424
StyI CCWWGG 5 cut(s) 307, 348, 363, 446, 671
TaaI ACNGT 1 cut(s) 437
TaiI ACGT 2 cut(s) 224, 342
TaqI TCGA 5 cut(s) 265, 388, 637, 848, 879
TasI AATT 7 cut(s) 97, 175, 300, 507, 658, 743, 774
TauI GCSGC 1 cut(s) 888
TfiI GAWTC 1 cut(s) 907
TscAI CASTG 2 cut(s) 187, 829
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 2 cut(s) 109, 146
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 6 cut(s) 90, 110, 681, 756, 865, 911
TspGWI ACGGA 1 cut(s) 529
TspRI CASTG 2 cut(s) 187, 829
Van91I CCANNNNNTGG 2 cut(s) 129, 447
VpaK11BI GGWCC 3 cut(s) 450, 612, 675
XapI RAATTY 3 cut(s) 97, 175, 743
XceI RCATGY 1 cut(s) 405
XspI CTAG 1 cut(s) 383
ZraI GACGTC 1 cut(s) 340
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.