FvH4_7g29723

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001157
Physical Location & Seq
Forward (+)
21817349 .. 21818602
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29723.t1

Sequence Viewer

Length: 1254 bp
ATGGACCCAGAAAAAATCAGCTGCATTGTCAACTGGCCCAAGCCCAAGACAATTAAAGCATTGAGAGGATTCCTAGGTCTAGCGGGGTACTACCGCCGTTTTGTCAGGCATTTCAGCATCATTGCACGTCCTATCACTGATATGCTCAAGAAGGAGAACTTTGTATGGTCGTCAGCTTCCGAGCAAGCTTTCGTCGATCTCAAATCAGCCATCACCTCAGCTCCGGTCTTAGCTCTGCCCAACTTCAATGAGGAGTTCTTCATCGAAACAGATGCCAGTGGATTGGGGGTCGGTGCCGTGCTCTCTCAAAGCAAACACCCCATCGAATTCCTCAGTCAAGCTCTCTCTCCACGTAACCAATTGCTCTCTACATATGAGAAGGAGATGTTGGCAGTGCTACATGCGATTTCTAAATGGCGTCAGTATCTTTTGGGTCACCACTTCACCATCATCACTGATCACCAACCTTTGGTACACATGTTTCAACAGCGGGTTCACACTCCGGCACAACAGAAGTGGATCTCCAAACTCTTGGGCTTCGATTATGCAGTCCAATACCGAGCGGGTAAGCTGAACGCTGTGGCTGATACCTTGTCCCGTCGTGCAGAATTTCTCAGTATTCACGGCATCTCAACCCCCATCTTTGATTGCCTTCCGGCAATTGACCAGGCCTGCCTCTCTGACCCGGACGCCAGCGCCATCATCAGTGCCTTGGCAACATGTGGCCATACACAGGCGCGATTTTCACTTGCTGCCGGTCGCCTCATGTATAAGGACAGGGTTTTTGTTCCTGCTACTTCCGATTGGCGCCCTAAGATCTTGTTTGAGTTCCATGATACTCTCACAGCCGGCCATTCCGGTTTCCTCAGAACTTTCAAGAGGTTGACACGCAATTTTGCGTGGCCTGGAGTTCGCCGGGATGTTAAGAAGTATGTGGCGTCATGTGATCAGTGTCAGCGAGTCAATTCGGAGCACCGCCACCCTTCTGGTCTTCTCCAACCACTTCCTATTCCTGACACAATTTGGACAGATATTGCAATGGATTTCGTCGATGGGTTACCGGCCTCTGCTGGCAAGACCTCTATTTTGGTAGTTGTTGATTGGTTGTCCAAATATGGTCATTTCGTGCCAATCAATCATCCTTATACTGCTGCTCAAGTCGCCGAGGTGTTCACCAAAGAGATATTTCGGCTCCATGGCATGCCTAAGACCATTGTCAGTGATCGGGACAGGGTGTTTATCTCAAATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

418

Amino Acids

47.02

Weight (kDa)

9.27

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 56 - 150 3.7e-30 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 81 - 180 1.4e-30 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 268 - 323 3e-20 Integrase zinc binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 293, 807
AccB7I CCANNNNNTGG 1 cut(s) 469
AccBSI CCGCTC 1 cut(s) 563
AccII CGCG 1 cut(s) 739
AciI CCGC 5 cut(s) 83, 94, 490, 563, 976
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 2 cut(s) 724, 850
AcsI RAATTY 3 cut(s) 326, 608, 1246
AcyI GRCGYC 4 cut(s) 418, 690, 808, 938
AfaI GTAC 2 cut(s) 89, 474
AfiI CCNNNNNNNGG 2 cut(s) 469, 733
AflIII ACRYGT 2 cut(s) 477, 719
AgsI TTSAA 3 cut(s) 247, 485, 877
AjiI CACGTC 1 cut(s) 128
AjnI CCWGG 2 cut(s) 666, 904
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 7 cut(s) 21, 176, 188, 221, 233, 341, 571
AluI AGCT 7 cut(s) 21, 176, 188, 221, 233, 341, 571
Alw21I GWGCWC 2 cut(s) 303, 975
AlwI GGATC 1 cut(s) 527
AoxI GGCC 6 cut(s) 35, 669, 724, 850, 902, 1062
ApeKI GCWGC 3 cut(s) 21, 752, 1151
ApoI RAATTY 3 cut(s) 326, 608, 1246
ArsI GACNNNNNNTTYG 4 cut(s) 767, 799, 1069, 1101
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 3 cut(s) 698, 739, 810
AspS9I GGNCC 2 cut(s) 4, 36
AsuC2I CCSGG 2 cut(s) 686, 917
AsuHPI GGTGA 5 cut(s) 205, 428, 436, 452, 1165
AvaII GGWCC 1 cut(s) 4
AvrII CCTAGG 1 cut(s) 73
BaeI ACNNNNGTAYC 2 cut(s) 464, 497
BalI TGGCCA 1 cut(s) 726
BanI GGYRCC 2 cut(s) 293, 807
BbsI GAAGAC 1 cut(s) 983
Bbv12I GWGCWC 2 cut(s) 303, 975
BbvCI CCTCAGC 1 cut(s) 217
BbvI GCAGC 3 cut(s) 8, 739, 1138
BccI CCATC 6 cut(s) 218, 329, 455, 647, 707, 1046
BceAI ACGGC 3 cut(s) 81, 281, 640
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 2 cut(s) 668, 906
BclI TGATCA 2 cut(s) 457, 946
BcnI CCSGG 2 cut(s) 686, 917
BfaI CTAG 3 cut(s) 74, 80, 1252
BfoI RGCGCY 2 cut(s) 699, 811
BglII AGATCT 1 cut(s) 816
BisI GCNGC 3 cut(s) 22, 753, 1152
BlnI CCTAGG 1 cut(s) 73
BlsI GCNGC 3 cut(s) 23, 754, 1153
Bme1390I CCNGG 4 cut(s) 668, 686, 906, 917
Bme18I GGWCC 1 cut(s) 4
BmgBI CACGTC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 4, 36
BmiI GGNNCC 4 cut(s) 6, 295, 809, 1193
BmrFI CCNGG 4 cut(s) 668, 686, 906, 917
BmsI GCATC 3 cut(s) 126, 262, 636
BoxI GACNNNNGTC 1 cut(s) 1214
BpiI GAAGAC 1 cut(s) 983
BpmI CTGGAG 1 cut(s) 927
Bpu10I CCTNAGC 1 cut(s) 217
BpuEI CTTGAG 2 cut(s) 131, 1140
BpuMI CCSGG 2 cut(s) 686, 917
BsaAI YACGTR 1 cut(s) 353
BsaHI GRCGYC 4 cut(s) 418, 690, 808, 938
BsaJI CCNNGG 4 cut(s) 73, 711, 1164, 1195
BsaWI WCCGGW 2 cut(s) 223, 857
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 2 cut(s) 469, 733
Bse118I RCCGGY 3 cut(s) 755, 848, 1060
Bse1I ACTGG 2 cut(s) 38, 276
Bse3DI GCAATG 2 cut(s) 120, 1044
BseBI CCWGG 2 cut(s) 668, 906
BseDI CCNNGG 4 cut(s) 73, 711, 1164, 1195
BseGI GGATG 2 cut(s) 925, 1138
BseLI CCNNNNNNNGG 2 cut(s) 469, 733
BseMI GCAATG 2 cut(s) 120, 1044
BseMII CTCAG 4 cut(s) 231, 346, 628, 880
BseNI ACTGG 2 cut(s) 38, 276
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 3 cut(s) 8, 739, 1138
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 739
Bsh1285I CGRYCG 1 cut(s) 760
BshFI GGCC 6 cut(s) 37, 671, 726, 852, 904, 1064
BshNI GGYRCC 2 cut(s) 293, 807
BsiEI CGRYCG 1 cut(s) 760
BsiHKAI GWGCWC 2 cut(s) 303, 975
BsiSI CCGG 9 cut(s) 224, 503, 656, 686, 756, 849, 858, 916, 1061
BslFI GGGAC 2 cut(s) 580, 1241
BslI CCNNNNNNNGG 2 cut(s) 469, 733
BsmFI GGGAC 2 cut(s) 580, 1241
BsnI GGCC 6 cut(s) 37, 671, 726, 852, 904, 1064
Bsp1286I GDGCHC 2 cut(s) 303, 975
Bsp143I GATC 6 cut(s) 196, 457, 519, 816, 946, 1222
Bsp19I CCATGG 1 cut(s) 1195
BspACI CCGC 5 cut(s) 83, 94, 490, 563, 976
BspANI GGCC 6 cut(s) 37, 671, 726, 852, 904, 1064
BspCNI CTCAG 4 cut(s) 230, 345, 627, 879
BspFNI CGCG 1 cut(s) 739
BspLI GGNNCC 4 cut(s) 6, 295, 809, 1193
BspPI GGATC 1 cut(s) 527
BspT107I GGYRCC 2 cut(s) 293, 807
BsrBI CCGCTC 1 cut(s) 563
BsrDI GCAATG 2 cut(s) 120, 1044
BsrFI RCCGGY 3 cut(s) 755, 848, 1060
BsrI ACTGG 2 cut(s) 38, 276
BssAI RCCGGY 3 cut(s) 755, 848, 1060
BssECI CCNNGG 4 cut(s) 73, 711, 1164, 1195
BssMI GATC 6 cut(s) 196, 457, 519, 816, 946, 1222
BssNI GRCGYC 4 cut(s) 418, 690, 808, 938
BssT1I CCWWGG 3 cut(s) 73, 711, 1195
Bst2UI CCWGG 2 cut(s) 668, 906
BstACI GRCGYC 4 cut(s) 418, 690, 808, 938
BstBAI YACGTR 1 cut(s) 353
BstC8I GCNNGC 6 cut(s) 186, 673, 694, 850, 1072, 1202
BstDEI CTNAG 7 cut(s) 217, 229, 332, 614, 813, 866, 1206
BstDSI CCRYGG 1 cut(s) 1195
BstEII GGTNACC 2 cut(s) 434, 1056
BstF5I GGATG 2 cut(s) 925, 1138
BstFNI CGCG 1 cut(s) 739
BstH2I RGCGCY 2 cut(s) 699, 811
BstHHI GCGC 3 cut(s) 698, 739, 810
BstKTI GATC 6 cut(s) 199, 460, 522, 819, 949, 1225
BstMBI GATC 6 cut(s) 196, 457, 519, 816, 946, 1222
BstMCI CGRYCG 1 cut(s) 760
BstMWI GCNNNNNNNGC 1 cut(s) 1160
BstNI CCWGG 2 cut(s) 668, 906
BstNSI RCATGY 4 cut(s) 404, 481, 723, 1204
BstPAI GACNNNNGTC 1 cut(s) 1214
BstPI GGTNACC 2 cut(s) 434, 1056
BstSCI CCNGG 4 cut(s) 666, 684, 904, 915
BstUI CGCG 1 cut(s) 739
BstV1I GCAGC 3 cut(s) 8, 739, 1138
BstV2I GAAGAC 1 cut(s) 983
BstX2I RGATCY 2 cut(s) 519, 816
BstXI CCANNNNNNTGG 3 cut(s) 283, 532, 986
BstYI RGATCY 2 cut(s) 519, 816
BsuRI GGCC 6 cut(s) 37, 671, 726, 852, 904, 1064
BtgI CCRYGG 1 cut(s) 1195
BtrI CACGTC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 925, 1138
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 7 cut(s) 135, 283, 399, 453, 712, 956, 1225
Cac8I GCNNGC 6 cut(s) 186, 673, 694, 850, 1072, 1202
CfoI GCGC 3 cut(s) 698, 739, 810
Cfr10I RCCGGY 3 cut(s) 755, 848, 1060
Cfr13I GGNCC 2 cut(s) 4, 36
CseI GACGC 3 cut(s) 407, 698, 927
Csp6I GTAC 2 cut(s) 88, 473
CspCI CAANNNNNGTGG 2 cut(s) 497, 532
CviAII CATG 8 cut(s) 401, 478, 720, 766, 833, 942, 1196, 1201
CviQI GTAC 2 cut(s) 88, 473
DdeI CTNAG 7 cut(s) 217, 229, 332, 614, 813, 866, 1206
DinI GGCGCC 1 cut(s) 809
DpnI GATC 6 cut(s) 198, 459, 521, 818, 948, 1224
DpnII GATC 6 cut(s) 196, 457, 519, 816, 946, 1222
EaeI YGGCCR 2 cut(s) 724, 850
Eco130I CCWWGG 3 cut(s) 73, 711, 1195
Eco147I AGGCCT 1 cut(s) 671
Eco47I GGWCC 1 cut(s) 4
Eco91I GGTNACC 2 cut(s) 434, 1056
EcoO65I GGTNACC 2 cut(s) 434, 1056
EcoRI GAATTC 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 666, 904
EcoT14I CCWWGG 3 cut(s) 73, 711, 1195
EgeI GGCGCC 1 cut(s) 809
EheI GGCGCC 1 cut(s) 809
ErhI CCWWGG 3 cut(s) 73, 711, 1195
FaeI CATG 8 cut(s) 404, 481, 723, 769, 836, 945, 1199, 1204
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 580, 1241
FatI CATG 8 cut(s) 400, 477, 719, 765, 832, 941, 1195, 1200
FauI CCCGC 3 cut(s) 76, 483, 556
FauNDI CATATG 1 cut(s) 373
FbaI TGATCA 2 cut(s) 457, 946
Fnu4HI GCNGC 3 cut(s) 22, 753, 1152
FokI GGATG 2 cut(s) 932, 1125
Fsp4HI GCNGC 3 cut(s) 22, 753, 1152
FspBI CTAG 3 cut(s) 74, 80, 1252
GlaI GCGC 3 cut(s) 697, 738, 809
GluI GCNGC 3 cut(s) 22, 753, 1152
GsuI CTGGAG 1 cut(s) 927
HaeII RGCGCY 2 cut(s) 699, 811
HaeIII GGCC 6 cut(s) 37, 671, 726, 852, 904, 1064
HapII CCGG 9 cut(s) 224, 503, 656, 686, 756, 849, 858, 916, 1061
HgaI GACGC 3 cut(s) 407, 698, 927
HhaI GCGC 3 cut(s) 698, 739, 810
Hin1I GRCGYC 4 cut(s) 418, 690, 808, 938
Hin1II CATG 8 cut(s) 404, 481, 723, 769, 836, 945, 1199, 1204
Hin6I GCGC 3 cut(s) 696, 737, 808
HinP1I GCGC 3 cut(s) 696, 737, 808
HincII GTYRAC 2 cut(s) 31, 885
HindII GTYRAC 2 cut(s) 31, 885
HindIII AAGCTT 1 cut(s) 186
HinfI GANTC 2 cut(s) 69, 960
HpaII CCGG 9 cut(s) 224, 503, 656, 686, 756, 849, 858, 916, 1061
HphI GGTGA 5 cut(s) 205, 428, 436, 452, 1165
Hpy166II GTNNAC 5 cut(s) 31, 475, 496, 885, 1173
Hpy188I TCNGA 5 cut(s) 181, 682, 802, 869, 970
Hpy188III TCNNGA 4 cut(s) 148, 877, 1013, 1226
Hpy8I GTNNAC 5 cut(s) 31, 475, 496, 885, 1173
Hpy99I CGWCG 3 cut(s) 197, 603, 1052
HpyAV CCTTC 4 cut(s) 145, 373, 662, 993
HpyCH4IV ACGT 2 cut(s) 127, 352
HpyCH4V TGCA 5 cut(s) 24, 125, 548, 605, 1037
HpyF10VI GCNNNNNNNGC 1 cut(s) 1160
HpyF3I CTNAG 7 cut(s) 217, 229, 332, 614, 813, 866, 1206
HpySE526I ACGT 2 cut(s) 127, 352
Hsp92I GRCGYC 4 cut(s) 418, 690, 808, 938
Hsp92II CATG 8 cut(s) 404, 481, 723, 769, 836, 945, 1199, 1204
HspAI GCGC 3 cut(s) 696, 737, 808
KasI GGCGCC 1 cut(s) 807
KroI GCCGGC 1 cut(s) 848
KroNI GCCGGC 1 cut(s) 850
Ksp22I TGATCA 2 cut(s) 457, 946
Kzo9I GATC 6 cut(s) 196, 457, 519, 816, 946, 1222
LmnI GCTCC 3 cut(s) 226, 970, 1197
Lsp1109I GCAGC 3 cut(s) 8, 739, 1138
LweI GCATC 3 cut(s) 126, 262, 636
MaeI CTAG 3 cut(s) 74, 80, 1252
MaeII ACGT 2 cut(s) 127, 352
MaeIII GTNAC 3 cut(s) 353, 434, 1056
MalI GATC 6 cut(s) 198, 459, 521, 818, 948, 1224
MbiI CCGCTC 1 cut(s) 563
MboI GATC 6 cut(s) 196, 457, 519, 816, 946, 1222
MboII GAAGA 2 cut(s) 250, 983
MfeI CAATTG 2 cut(s) 359, 660
MflI RGATCY 2 cut(s) 519, 816
MhlI GDGCHC 2 cut(s) 303, 975
MlsI TGGCCA 1 cut(s) 726
MluCI AATT 9 cut(s) 51, 326, 359, 608, 660, 892, 964, 1020, 1246
MluNI TGGCCA 1 cut(s) 726
Mly113I GGCGCC 1 cut(s) 808
MlyI GAGTC 1 cut(s) 969
MmeI TCCRAC 1 cut(s) 1021
Mox20I TGGCCA 1 cut(s) 726
MroNI GCCGGC 1 cut(s) 848
MscI TGGCCA 1 cut(s) 726
MseI TTAA 2 cut(s) 54, 924
MslI CAYNNNNRTG 1 cut(s) 140
Msp20I TGGCCA 1 cut(s) 726
MspA1I CMGCKG 2 cut(s) 21, 490
MspI CCGG 9 cut(s) 224, 503, 656, 686, 756, 849, 858, 916, 1061
MspR9I CCNGG 4 cut(s) 668, 686, 906, 917
MunI CAATTG 2 cut(s) 359, 660
MvaI CCWGG 2 cut(s) 668, 906
MvnI CGCG 1 cut(s) 739
MwoI GCNNNNNNNGC 1 cut(s) 1160
NaeI GCCGGC 1 cut(s) 850
NarI GGCGCC 1 cut(s) 808
NciI CCSGG 2 cut(s) 686, 917
NcoI CCATGG 1 cut(s) 1195
NdeI CATATG 1 cut(s) 373
NdeII GATC 6 cut(s) 196, 457, 519, 816, 946, 1222
NgoMIV GCCGGC 1 cut(s) 848
NlaIII CATG 8 cut(s) 404, 481, 723, 769, 836, 945, 1199, 1204
NlaIV GGNNCC 4 cut(s) 6, 295, 809, 1193
NmeAIII GCCGAG 1 cut(s) 1189
NmuCI GTSAC 1 cut(s) 434
NspI RCATGY 4 cut(s) 404, 481, 723, 1204
PaeI GCATGC 1 cut(s) 1204
PceI AGGCCT 1 cut(s) 671
PciI ACATGT 2 cut(s) 477, 719
PdiI GCCGGC 1 cut(s) 850
PfeI GAWTC 1 cut(s) 69
PflMI CCANNNNNTGG 1 cut(s) 469
PkrI GCNGC 3 cut(s) 23, 754, 1153
PleI GAGTC 1 cut(s) 968
PluTI GGCGCC 1 cut(s) 811
PpsI GAGTC 1 cut(s) 968
Ppu21I YACGTR 1 cut(s) 353
PscI ACATGT 2 cut(s) 477, 719
PshAI GACNNNNGTC 1 cut(s) 1214
Psp6I CCWGG 2 cut(s) 666, 904
PspEI GGTNACC 2 cut(s) 434, 1056
PspGI CCWGG 2 cut(s) 666, 904
PspN4I GGNNCC 4 cut(s) 6, 295, 809, 1193
PspPI GGNCC 2 cut(s) 4, 36
PsuI RGATCY 2 cut(s) 519, 816
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 2 cut(s) 89, 474
RsaNI GTAC 2 cut(s) 88, 473
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 2 cut(s) 54, 924
SatI GCNGC 3 cut(s) 22, 753, 1152
Sau3AI GATC 6 cut(s) 196, 457, 519, 816, 946, 1222
Sau96I GGNCC 2 cut(s) 4, 36
SchI GAGTC 1 cut(s) 969
ScrFI CCNGG 4 cut(s) 668, 686, 906, 917
SduI GDGCHC 2 cut(s) 303, 975
SfaNI GCATC 3 cut(s) 126, 262, 636
SfoI GGCGCC 1 cut(s) 809
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 2 cut(s) 146, 1155
SmoI CTYRAG 2 cut(s) 146, 1155
SphI GCATGC 1 cut(s) 1204
Sse9I AATT 9 cut(s) 51, 326, 359, 608, 660, 892, 964, 1020, 1246
SseBI AGGCCT 1 cut(s) 671
SsiI CCGC 5 cut(s) 83, 94, 490, 563, 976
SspDI GGCGCC 1 cut(s) 807
SspMI CTAG 3 cut(s) 74, 80, 1252
StuI AGGCCT 1 cut(s) 671
StyD4I CCNGG 4 cut(s) 666, 684, 904, 915
StyI CCWWGG 3 cut(s) 73, 711, 1195
TaiI ACGT 2 cut(s) 130, 355
TaqI TCGA 5 cut(s) 195, 264, 324, 540, 1050
TasI AATT 9 cut(s) 51, 326, 359, 608, 660, 892, 964, 1020, 1246
TfiI GAWTC 1 cut(s) 69
Tru1I TTAA 2 cut(s) 54, 924
Tru9I TTAA 2 cut(s) 54, 924
TscAI CASTG 7 cut(s) 142, 283, 399, 460, 712, 956, 1225
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 3 cut(s) 21, 752, 1151
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 1 cut(s) 250
TspRI CASTG 7 cut(s) 142, 283, 399, 460, 712, 956, 1225
Van91I CCANNNNNTGG 1 cut(s) 469
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 3 cut(s) 326, 608, 1246
XceI RCATGY 4 cut(s) 404, 481, 723, 1204
XmaJI CCTAGG 1 cut(s) 73
XspI CTAG 3 cut(s) 74, 80, 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.