Rroxscaffold_5G00378120

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001157
Physical Location & Seq
Forward (+)
58632063 .. 58643898
11836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00378120.1

Sequence Viewer

Length: 1362 bp
ATGGCCTTTACCAAGCTCGCCGGAAGGGCAAGAGGTTGGTCGGAGTCCGAACTGCTCCCCATCTTTCTCAGTGGGTTGAAGCCGGAAATTCGGCACGACGTGATTGCCTTGGAGCCCCAAACCCTGGTATCTGCTCAGCGCCTGGCACACCGTTTTGAAATAAAGCTGGCCGACATTCGTTCCTCTCGACAAAGCCGTACTTCTTGGCATCATTCTCCAAGGCCCACTCAATTCTCACACCTCTACTCTGTCCCCACTACAAGCCAAATATCCCTTCCCCACACTCCCAATTCCACCACCTTACCCAATACCCATTCCTTTCCTACCAACCAGAAGCCAAAACATGAGGGCCCATTCCGCAAATGGTCTCCAGCGGAACAGAGGGAACGCAGAGCTCAGGGCCTCTGCTACAGTTGCGATGAGACTTGGTCCAAATCCCACATCTGCAAGAAACCCGTCATGGCCATCCTGGAATCCTTAGCACCGTTGGAAGAGCAACCCTCCGAGGGCTCTCATGAGGAGGACCACCACTCCGACGAGGACCAAGTCCTTCCCCTCCATGCCATCACGAACACAACCATCAGTGAAATGATGAGATTTGAAGGCAACATCAATGGCAGCCCCGTCGCCGTTTTCGTCGATTGTGGCTCCGCTAGAAGTTTTTTGAGCCCCGCCGTCGCCCACCGTCTTGGCCTCACTATCCGGCCGGCGTCGAAACTCCAATTCACCTCAGCTTGTAACCAACCCATTTCACCCTCCGAGCAAGCAGTCGACGTCACGGTCCAAATTCAGGACTATCAATTTACTAGCTCTTTCCTCTTGCTCCAGGTCCCTGGTTGTGAACTATTGTTGGGAGCTGAATGGCTCGATACTTTAGGCGTTATTGGGTGGCATTTCTTGGATAAGGTCATGATGTTCACGGCTCAAGGAAAGTGCCACGTGCTTCAAGGCCTCACCAAAAAGCCCAGGCGAACTGACCAAACTACCATCATGACCATCTTACCTTCCGACCAACAAGAATTCTTTTTCCACCCTCCCAAGCTCACTACTTCTACACCAACCACATACCCTCTACCACTCCAAACTCTTTTGGACACCCACAGAGACTTATTCTCCGCACCCACCTCCCTACCCCCACGTCGAACCTTTGATCACCGTATCCCTTTACTGCCCAACACCGGCCCAATCAATGTCCGACCTTATCGATATGCACATTCTCAAAAAGAAGAACTGGAAATACAAGTTCAGGAAATGCTAGCCAATGGGTTGATACGGCCTAGCCGAAGTCCTTTCTTCGCCGGTACTTTTGGTCAAGAAAAAGGAAGGCACTTGGCGGTTTTGTGTGGATTACCGACAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

453

Amino Acids

50.89

Weight (kDa)

8.12

Isoelectric Point (pI)

54.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag-asp_proteas PF13975 201 - 289 5.6e-09 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 201 - 286 7.9e-08 Aspartyl protease
RVP_2 PF08284 203 - 290 3.5e-07 Retroviral aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 779
AatII GACGTC 1 cut(s) 777
AccB7I CCANNNNNTGG 1 cut(s) 124
AccI GTMKAC 1 cut(s) 771
AciI CCGC 6 cut(s) 358, 374, 651, 672, 1116, 1334
AcoI YGGCCR 3 cut(s) 168, 462, 704
AcsI RAATTY 3 cut(s) 87, 786, 1019
AcvI CACGTG 1 cut(s) 940
AcyI GRCGYC 2 cut(s) 710, 774
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 2 cut(s) 199, 1303
AfiI CCNNNNNNNGG 4 cut(s) 124, 506, 790, 1178
AgsI TTSAA 4 cut(s) 79, 158, 602, 947
AjiI CACGTC 2 cut(s) 100, 1139
AjnI CCWGG 6 cut(s) 123, 141, 468, 825, 832, 965
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AluBI AGCT 7 cut(s) 16, 166, 395, 734, 810, 857, 1042
AluI AGCT 7 cut(s) 16, 166, 395, 734, 810, 857, 1042
Alw21I GWGCWC 1 cut(s) 397
Alw26I GTCTC 3 cut(s) 372, 416, 1098
AlwNI CAGNNNCTG 1 cut(s) 142
ApaI GGGCCC 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 618
ApoI RAATTY 3 cut(s) 87, 786, 1019
AspLEI GCGC 1 cut(s) 141
AsuHPI GGTGA 4 cut(s) 718, 744, 946, 1145
AsuNHI GCTAGC 1 cut(s) 1255
AvaII GGWCC 5 cut(s) 429, 523, 541, 781, 829
BaeGI GKGCMC 1 cut(s) 353
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BalI TGGCCA 1 cut(s) 464
BanII GRGCYC 5 cut(s) 117, 353, 397, 512, 671
BbrPI CACGTG 1 cut(s) 940
Bbv12I GWGCWC 1 cut(s) 397
BbvCI CCTCAGC 1 cut(s) 730
BbvI GCAGC 1 cut(s) 630
BccI CCATC 6 cut(s) 68, 473, 572, 587, 995, 1004
BceAI ACGGC 5 cut(s) 180, 614, 659, 936, 1289
BcgI CGANNNNNNTGC 4 cut(s) 86, 120, 607, 641
BciT130I CCWGG 6 cut(s) 125, 143, 470, 827, 834, 967
BciVI GTATCC 1 cut(s) 1169
BclI TGATCA 1 cut(s) 1150
BcoDI GTCTC 3 cut(s) 372, 416, 1098
BfaI CTAG 4 cut(s) 654, 807, 1256, 1278
BfmI CTRYAG 1 cut(s) 409
BfoI RGCGCY 1 cut(s) 142
BfuI GTATCC 1 cut(s) 1169
BglI GCCNNNNNGGC 1 cut(s) 26
BisI GCNGC 1 cut(s) 619
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 620
Bme1390I CCNGG 6 cut(s) 125, 143, 470, 827, 834, 967
Bme18I GGWCC 5 cut(s) 429, 523, 541, 781, 829
BmgBI CACGTC 2 cut(s) 100, 1139
BmiI GGNNCC 4 cut(s) 114, 351, 649, 831
BmrFI CCNGG 6 cut(s) 125, 143, 470, 827, 834, 967
BmsI GCATC 1 cut(s) 217
BmtI GCTAGC 1 cut(s) 1259
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 2 cut(s) 354, 809
Bpu10I CCTNAGC 3 cut(s) 396, 478, 730
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 909
Bsa29I ATCGAT 1 cut(s) 1204
BsaAI YACGTR 1 cut(s) 940
BsaHI GRCGYC 2 cut(s) 710, 774
BsaI GGTCTC 1 cut(s) 372
BsaJI CCNNGG 6 cut(s) 108, 123, 218, 504, 832, 965
BsaXI ACNNNNNCTCC 4 cut(s) 515, 545, 1097, 1127
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 506, 790, 1178
Bse118I RCCGGY 3 cut(s) 706, 1178, 1298
Bse1I ACTGG 1 cut(s) 1236
BseBI CCWGG 6 cut(s) 125, 143, 470, 827, 834, 967
BseCI ATCGAT 1 cut(s) 1204
BseDI CCNNGG 6 cut(s) 108, 123, 218, 504, 832, 965
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 4 cut(s) 124, 506, 790, 1178
BseMII CTCAG 4 cut(s) 82, 149, 410, 744
BseNI ACTGG 1 cut(s) 1236
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 1 cut(s) 353
BseX3I CGGCCG 1 cut(s) 704
BseXI GCAGC 1 cut(s) 630
Bsh1285I CGRYCG 1 cut(s) 707
BshVI ATCGAT 1 cut(s) 1204
BsiEI CGRYCG 1 cut(s) 707
BsiHKAI GWGCWC 1 cut(s) 397
BsiSI CCGG 6 cut(s) 21, 83, 703, 707, 1179, 1299
BslFI GGGAC 2 cut(s) 236, 815
BslI CCNNNNNNNGG 4 cut(s) 124, 506, 790, 1178
BsmAI GTCTC 3 cut(s) 372, 416, 1098
BsmFI GGGAC 2 cut(s) 236, 815
Bso31I GGTCTC 1 cut(s) 372
Bsp120I GGGCCC 1 cut(s) 349
Bsp1286I GDGCHC 5 cut(s) 117, 353, 397, 512, 671
Bsp143I GATC 1 cut(s) 1150
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 6 cut(s) 358, 374, 651, 672, 1116, 1334
BspCNI CTCAG 4 cut(s) 81, 148, 409, 743
BspDI ATCGAT 1 cut(s) 1204
BspHI TCATGA 3 cut(s) 514, 909, 990
BspLI GGNNCC 4 cut(s) 114, 351, 649, 831
BspOI GCTAGC 1 cut(s) 1259
BspQI GCTCTTC 1 cut(s) 486
BspTNI GGTCTC 1 cut(s) 372
BsrFI RCCGGY 3 cut(s) 706, 1178, 1298
BsrI ACTGG 1 cut(s) 1236
BssAI RCCGGY 3 cut(s) 706, 1178, 1298
BssECI CCNNGG 6 cut(s) 108, 123, 218, 504, 832, 965
BssMI GATC 1 cut(s) 1150
BssNI GRCGYC 2 cut(s) 710, 774
BssT1I CCWWGG 2 cut(s) 108, 218
Bst2UI CCWGG 6 cut(s) 125, 143, 470, 827, 834, 967
Bst4CI ACNGT 6 cut(s) 152, 413, 486, 686, 781, 1157
Bst6I CTCTTC 1 cut(s) 486
BstACI GRCGYC 2 cut(s) 710, 774
BstBAI YACGTR 1 cut(s) 940
BstC8I GCNNGC 5 cut(s) 18, 168, 708, 765, 1257
BstDEI CTNAG 5 cut(s) 68, 135, 396, 478, 730
BstF5I GGATG 1 cut(s) 465
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 1 cut(s) 1153
BstMAI GTCTC 3 cut(s) 372, 416, 1098
BstMBI GATC 1 cut(s) 1150
BstMCI CGRYCG 1 cut(s) 707
BstMWI GCNNNNNNNGC 3 cut(s) 26, 357, 414
BstNI CCWGG 6 cut(s) 125, 143, 470, 827, 834, 967
BstSCI CCNGG 6 cut(s) 123, 141, 468, 825, 832, 965
BstSFI CTRYAG 1 cut(s) 409
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 1 cut(s) 630
BstXI CCANNNNNNTGG 2 cut(s) 689, 833
BstZI CGGCCG 1 cut(s) 704
Bsu15I ATCGAT 1 cut(s) 1204
BsuI GTATCC 1 cut(s) 1169
BsuTUI ATCGAT 1 cut(s) 1204
BtgZI GCGATG 1 cut(s) 432
BtrI CACGTC 2 cut(s) 100, 1139
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 76, 589
Cac8I GCNNGC 5 cut(s) 18, 168, 708, 765, 1257
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 3 cut(s) 514, 909, 990
CfoI GCGC 1 cut(s) 141
Cfr10I RCCGGY 3 cut(s) 706, 1178, 1298
ClaI ATCGAT 1 cut(s) 1204
CseI GACGC 1 cut(s) 699
Csp6I GTAC 2 cut(s) 198, 1302
CviAII CATG 6 cut(s) 344, 460, 515, 560, 910, 991
CviQI GTAC 2 cut(s) 198, 1302
DdeI CTNAG 5 cut(s) 68, 135, 396, 478, 730
DpnI GATC 1 cut(s) 1152
DpnII GATC 1 cut(s) 1150
DraIII CACNNNGTG 1 cut(s) 100
DrdI GACNNNNNNGTC 1 cut(s) 779
DseDI GACNNNNNNGTC 1 cut(s) 779
EaeI YGGCCR 3 cut(s) 168, 462, 704
EagI CGGCCG 1 cut(s) 704
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
Ecl136II GAGCTC 1 cut(s) 395
EclXI CGGCCG 1 cut(s) 704
Eco130I CCWWGG 2 cut(s) 108, 218
Eco147I AGGCCT 1 cut(s) 951
Eco24I GRGCYC 5 cut(s) 117, 353, 397, 512, 671
Eco31I GGTCTC 1 cut(s) 372
Eco47I GGWCC 5 cut(s) 429, 523, 541, 781, 829
Eco52I CGGCCG 1 cut(s) 704
Eco53kI GAGCTC 1 cut(s) 395
Eco72I CACGTG 1 cut(s) 940
EcoICRI GAGCTC 1 cut(s) 395
EcoO109I RGGNCCY 3 cut(s) 349, 400, 829
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 6 cut(s) 123, 141, 468, 825, 832, 965
EcoT14I CCWWGG 2 cut(s) 108, 218
EcoT38I GRGCYC 5 cut(s) 117, 353, 397, 512, 671
ErhI CCWWGG 2 cut(s) 108, 218
FaeI CATG 6 cut(s) 347, 463, 518, 563, 913, 994
FaiI YATR 8 cut(s) 345, 461, 516, 561, 911, 992, 1066, 1209
FalI AAGNNNNNCTT 2 cut(s) 184, 216
FaqI GGGAC 2 cut(s) 236, 815
FatI CATG 6 cut(s) 343, 459, 514, 559, 909, 990
FauI CCCGC 1 cut(s) 679
FbaI TGATCA 1 cut(s) 1150
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 1 cut(s) 619
FokI GGATG 1 cut(s) 452
FriOI GRGCYC 5 cut(s) 117, 353, 397, 512, 671
Fsp4HI GCNGC 1 cut(s) 619
FspBI CTAG 4 cut(s) 654, 807, 1256, 1278
GlaI GCGC 1 cut(s) 140
GluI GCNGC 1 cut(s) 619
GsuI CTGGAG 2 cut(s) 354, 809
HaeII RGCGCY 1 cut(s) 142
HapII CCGG 6 cut(s) 21, 83, 703, 707, 1179, 1299
HgaI GACGC 1 cut(s) 699
HhaI GCGC 1 cut(s) 141
Hin1I GRCGYC 2 cut(s) 710, 774
Hin1II CATG 6 cut(s) 347, 463, 518, 563, 913, 994
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 2 cut(s) 44, 473
HpaII CCGG 6 cut(s) 21, 83, 703, 707, 1179, 1299
HphI GGTGA 4 cut(s) 718, 744, 946, 1145
Hpy166II GTNNAC 3 cut(s) 772, 842, 918
Hpy188I TCNGA 7 cut(s) 43, 49, 505, 535, 760, 1009, 1196
Hpy188III TCNNGA 8 cut(s) 186, 515, 568, 791, 910, 991, 1247, 1313
Hpy8I GTNNAC 3 cut(s) 772, 842, 918
Hpy99I CGWCG 8 cut(s) 101, 539, 629, 641, 680, 715, 776, 1143
HpyAV CCTTC 6 cut(s) 18, 284, 560, 596, 1014, 1317
HpyCH4III ACNGT 6 cut(s) 152, 413, 486, 686, 781, 1157
HpyCH4IV ACGT 4 cut(s) 99, 774, 939, 1138
HpyCH4V TGCA 2 cut(s) 447, 1211
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 357, 414
HpyF3I CTNAG 5 cut(s) 68, 135, 396, 478, 730
HpySE526I ACGT 4 cut(s) 99, 774, 939, 1138
Hsp92I GRCGYC 2 cut(s) 710, 774
Hsp92II CATG 6 cut(s) 347, 463, 518, 563, 913, 994
HspAI GCGC 1 cut(s) 139
KroI GCCGGC 1 cut(s) 706
KroNI GCCGGC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 1150
Kzo9I GATC 1 cut(s) 1150
LguI GCTCTTC 1 cut(s) 486
LmnI GCTCC 5 cut(s) 60, 112, 653, 828, 854
Lsp1109I GCAGC 1 cut(s) 630
LweI GCATC 1 cut(s) 217
MaeI CTAG 4 cut(s) 654, 807, 1256, 1278
MaeII ACGT 4 cut(s) 99, 774, 939, 1138
MaeIII GTNAC 2 cut(s) 737, 775
MalI GATC 1 cut(s) 1152
MboI GATC 1 cut(s) 1150
MboII GAAGA 3 cut(s) 503, 1238, 1285
MhlI GDGCHC 5 cut(s) 117, 353, 397, 512, 671
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 7 cut(s) 87, 230, 289, 722, 786, 800, 1019
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 5 cut(s) 21, 468, 558, 1032, 1219
Mox20I TGGCCA 1 cut(s) 464
MroNI GCCGGC 1 cut(s) 706
MscI TGGCCA 1 cut(s) 464
MseI TTAA 1 cut(s) 1360
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 6 cut(s) 21, 83, 703, 707, 1179, 1299
MspR9I CCNGG 6 cut(s) 125, 143, 470, 827, 834, 967
MvaI CCWGG 6 cut(s) 125, 143, 470, 827, 834, 967
MwoI GCNNNNNNNGC 3 cut(s) 26, 357, 414
NaeI GCCGGC 1 cut(s) 708
NdeII GATC 1 cut(s) 1150
NgoMIV GCCGGC 1 cut(s) 706
NheI GCTAGC 1 cut(s) 1255
NlaIII CATG 6 cut(s) 347, 463, 518, 563, 913, 994
NlaIV GGNNCC 4 cut(s) 114, 351, 649, 831
NmuCI GTSAC 1 cut(s) 775
PagI TCATGA 3 cut(s) 514, 909, 990
PceI AGGCCT 1 cut(s) 951
PciSI GCTCTTC 1 cut(s) 486
PcsI WCGNNNNNNNCGW 4 cut(s) 184, 193, 633, 1279
PdiI GCCGGC 1 cut(s) 708
PfeI GAWTC 1 cut(s) 473
PflFI GACNNNGTC 2 cut(s) 427, 545
PflMI CCANNNNNTGG 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 468
PkrI GCNGC 1 cut(s) 620
PleI GAGTC 1 cut(s) 52
PmaCI CACGTG 1 cut(s) 940
PmlI CACGTG 1 cut(s) 940
PpsI GAGTC 1 cut(s) 52
Ppu21I YACGTR 1 cut(s) 940
PpuMI RGGWCCY 1 cut(s) 829
Psp124BI GAGCTC 1 cut(s) 397
Psp5II RGGWCCY 1 cut(s) 829
Psp6I CCWGG 6 cut(s) 123, 141, 468, 825, 832, 965
PspCI CACGTG 1 cut(s) 940
PspGI CCWGG 6 cut(s) 123, 141, 468, 825, 832, 965
PspN4I GGNNCC 4 cut(s) 114, 351, 649, 831
PspOMI GGGCCC 1 cut(s) 349
PspPPI RGGWCCY 1 cut(s) 829
PsrI GAACNNNNNNTAC 2 cut(s) 1221, 1253
PstNI CAGNNNCTG 1 cut(s) 142
PsyI GACNNNGTC 2 cut(s) 427, 545
RsaI GTAC 2 cut(s) 199, 1303
RsaNI GTAC 2 cut(s) 198, 1302
SacI GAGCTC 1 cut(s) 397
SalI GTCGAC 1 cut(s) 770
SapI GCTCTTC 1 cut(s) 486
SaqAI TTAA 1 cut(s) 1360
SatI GCNGC 1 cut(s) 619
Sau3AI GATC 1 cut(s) 1150
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 6 cut(s) 125, 143, 470, 827, 834, 967
SduI GDGCHC 5 cut(s) 117, 353, 397, 512, 671
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 409
SinI GGWCC 5 cut(s) 429, 523, 541, 781, 829
SmlI CTYRAG 1 cut(s) 924
SmoI CTYRAG 1 cut(s) 924
Sse9I AATT 7 cut(s) 87, 230, 289, 722, 786, 800, 1019
SseBI AGGCCT 1 cut(s) 951
SsiI CCGC 6 cut(s) 358, 374, 651, 672, 1116, 1334
SspMI CTAG 4 cut(s) 654, 807, 1256, 1278
SstI GAGCTC 1 cut(s) 397
StuI AGGCCT 1 cut(s) 951
StyD4I CCNGG 6 cut(s) 123, 141, 468, 825, 832, 965
StyI CCWWGG 2 cut(s) 108, 218
TaaI ACNGT 6 cut(s) 152, 413, 486, 686, 781, 1157
TaiI ACGT 4 cut(s) 102, 777, 942, 1141
TaqI TCGA 7 cut(s) 187, 639, 713, 771, 867, 1141, 1204
TasI AATT 7 cut(s) 87, 230, 289, 722, 786, 800, 1019
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 1 cut(s) 1360
Tru9I TTAA 1 cut(s) 1360
TscAI CASTG 2 cut(s) 76, 589
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 1 cut(s) 618
Tsp45I GTSAC 1 cut(s) 775
TspRI CASTG 2 cut(s) 76, 589
Tth111I GACNNNGTC 2 cut(s) 427, 545
Van91I CCANNNNNTGG 1 cut(s) 124
VpaK11BI GGWCC 5 cut(s) 429, 523, 541, 781, 829
XapI RAATTY 3 cut(s) 87, 786, 1019
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmiI GTMKAC 1 cut(s) 771
XspI CTAG 4 cut(s) 654, 807, 1256, 1278
ZraI GACGTC 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.