RchiOBHm_Chr1g0313301

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001157
Physical Location & Seq
Forward (+)
428496 .. 430089
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54412

Sequence Viewer

Length: 636 bp
ATGCATTACGGCATCACAGCCAAGCCTCTCACCGAATTGCTTAAAATCGACAGTTTCAAGAGGAACCCAGCAGCGGAAACGGTCTTCGAGACGCTCAAACAAGCCTTGATGACGGCTCCGGTGTTGGCACTGCCGGATTTCTCTCTTCCATTCACAATAGAAACAGATGCATGTGGATTGGGTATAGGGGCAGTCCTCTCGCAGCGTAGGCACCCAATTGCATACTTGAGTAAGCCTCTCTCTGCTCGAAATCAGCTACTATCGGTGTATGACAAGGAGATGCTCGCCATCTGGCACTCCATTGACAAATGGCCCCCATATCTCTTGGGCCATCAATTCACCATCATCACCGATCACCAGACCTTGAAGCATCTCTTGGACCAGCGTATATCAACACCCTCGCAGCACAAGTGGCTTGCTAAACTCATGGGCTACCATTATTCAATCAAGTACAGACCTGGCAGTTTGAATACAGTTCCTGATTTACTCTTGAGGCGTCCTGAATTGTGCTCAATCGCGGCTATCTTTGCTCCGGTGTTTGACAGTCTGGAACAAATATCTCAAGCATGCCTTATCTCAGTACATTGTTGCATTGTGCAGAATGGTGGTACAACTCCACTTATCATTCCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

211

Amino Acids

23.61

Weight (kDa)

8.58

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 23 - 115 4.2e-29 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 46 - 145 2.1e-29 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 210
AccII CGCG 1 cut(s) 518
AciI CCGC 2 cut(s) 74, 518
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 3 cut(s) 452, 582, 610
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 4 cut(s) 58, 367, 444, 469
AjnI CCWGG 1 cut(s) 457
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 1 cut(s) 256
AluI AGCT 1 cut(s) 256
Alw21I GWGCWC 1 cut(s) 512
Alw26I GTCTC 1 cut(s) 83
AlwNI CAGNNNCTG 1 cut(s) 479
AoxI GGCC 2 cut(s) 311, 328
ApeKI GCWGC 3 cut(s) 71, 202, 403
AspS9I GGNCC 3 cut(s) 312, 328, 379
AsuHPI GGTGA 4 cut(s) 22, 331, 340, 347
AvaII GGWCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 512
BbvI GCAGC 3 cut(s) 83, 214, 415
BccI CCATC 3 cut(s) 296, 339, 350
BceAI ACGGC 2 cut(s) 25, 129
BcgI CGANNNNNNTGC 2 cut(s) 180, 214
BciT130I CCWGG 1 cut(s) 459
BcoDI GTCTC 1 cut(s) 83
BisI GCNGC 4 cut(s) 72, 203, 404, 519
BlsI GCNGC 4 cut(s) 73, 204, 405, 520
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 3 cut(s) 312, 328, 379
BmiI GGNNCC 4 cut(s) 65, 117, 212, 314
BmrFI CCNGG 1 cut(s) 459
BmsI GCATC 4 cut(s) 21, 157, 270, 379
BpiI GAAGAC 1 cut(s) 76
BplI GAGNNNNNCTC 2 cut(s) 220, 252
BpuEI CTTGAG 3 cut(s) 247, 511, 546
BsaHI GRCGYC 1 cut(s) 496
BsaWI WCCGGW 2 cut(s) 118, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 73
BseBI CCWGG 1 cut(s) 459
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 591
BseXI GCAGC 3 cut(s) 83, 214, 415
BseYI CCCAGC 1 cut(s) 67
BsgI GTGCAG 1 cut(s) 617
Bsh1236I CGCG 1 cut(s) 518
BshFI GGCC 2 cut(s) 313, 330
BshNI GGYRCC 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 512
BsiSI CCGG 3 cut(s) 119, 134, 533
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 83
BsmBI CGTCTC 1 cut(s) 83
BsnI GGCC 2 cut(s) 313, 330
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 2 cut(s) 74, 518
BspANI GGCC 2 cut(s) 313, 330
BspCNI CTCAG 1 cut(s) 590
BspFNI CGCG 1 cut(s) 518
BspLI GGNNCC 4 cut(s) 65, 117, 212, 314
BspT107I GGYRCC 1 cut(s) 210
BssMI GATC 1 cut(s) 352
BssNI GRCGYC 1 cut(s) 496
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 4 cut(s) 53, 82, 475, 545
Bst6I CTCTTC 1 cut(s) 150
BstACI GRCGYC 1 cut(s) 496
BstC8I GCNNGC 3 cut(s) 285, 417, 568
BstDEI CTNAG 1 cut(s) 577
BstFNI CGCG 1 cut(s) 518
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 3 cut(s) 208, 412, 527
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 2 cut(s) 174, 570
BstSCI CCNGG 1 cut(s) 457
BstUI CGCG 1 cut(s) 518
BstV1I GCAGC 3 cut(s) 83, 214, 415
BstV2I GAAGAC 1 cut(s) 76
BsuRI GGCC 2 cut(s) 313, 330
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 128
Cac8I GCNNGC 3 cut(s) 285, 417, 568
CaiI CAGNNNCTG 1 cut(s) 479
Cfr13I GGNCC 3 cut(s) 312, 328, 379
CseI GACGC 2 cut(s) 100, 485
Csp6I GTAC 3 cut(s) 451, 581, 609
CviAII CATG 3 cut(s) 171, 427, 567
CviQI GTAC 3 cut(s) 451, 581, 609
DdeI CTNAG 1 cut(s) 577
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 457
EcoT22I ATGCAT 2 cut(s) 6, 172
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 3 cut(s) 174, 430, 570
FaiI YATR 9 cut(s) 172, 185, 223, 270, 319, 389, 428, 568, 634
FalI AAGNNNNNCTT 4 cut(s) 359, 391, 555, 587
FatI CATG 3 cut(s) 170, 426, 566
Fnu4HI GCNGC 4 cut(s) 72, 203, 404, 519
Fsp4HI GCNGC 4 cut(s) 72, 203, 404, 519
GluI GCNGC 4 cut(s) 72, 203, 404, 519
GsaI CCCAGC 1 cut(s) 71
HaeIII GGCC 2 cut(s) 313, 330
HapII CCGG 3 cut(s) 119, 134, 533
HgaI GACGC 2 cut(s) 100, 485
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 3 cut(s) 174, 430, 570
HpaII CCGG 3 cut(s) 119, 134, 533
HphI GGTGA 4 cut(s) 22, 331, 340, 347
Hpy188III TCNNGA 6 cut(s) 58, 88, 479, 490, 500, 548
HpyCH4III ACNGT 4 cut(s) 53, 82, 475, 545
HpyCH4V TGCA 5 cut(s) 4, 170, 221, 591, 598
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 412, 527
HpyF3I CTNAG 1 cut(s) 577
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 3 cut(s) 174, 430, 570
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 2 cut(s) 121, 535
Lsp1109I GCAGC 3 cut(s) 83, 214, 415
LweI GCATC 4 cut(s) 21, 157, 270, 379
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 2 cut(s) 76, 137
MfeI CAATTG 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 512
MluCI AATT 4 cut(s) 35, 216, 335, 503
MnlI CCTC 6 cut(s) 36, 54, 206, 246, 409, 486
Mph1103I ATGCAT 2 cut(s) 6, 172
MseI TTAA 1 cut(s) 42
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 3 cut(s) 119, 134, 533
MspR9I CCNGG 1 cut(s) 459
MunI CAATTG 1 cut(s) 216
MvaI CCWGG 1 cut(s) 459
MvnI CGCG 1 cut(s) 518
MwoI GCNNNNNNNGC 3 cut(s) 208, 412, 527
NdeII GATC 1 cut(s) 352
NlaIII CATG 3 cut(s) 174, 430, 570
NlaIV GGNNCC 4 cut(s) 65, 117, 212, 314
NsiI ATGCAT 2 cut(s) 6, 172
NspI RCATGY 2 cut(s) 174, 570
PaeI GCATGC 1 cut(s) 570
PkrI GCNGC 4 cut(s) 73, 204, 405, 520
Psp6I CCWGG 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 4 cut(s) 65, 117, 212, 314
PspPI GGNCC 3 cut(s) 312, 328, 379
PstNI CAGNNNCTG 1 cut(s) 479
RsaI GTAC 3 cut(s) 452, 582, 610
RsaNI GTAC 3 cut(s) 451, 581, 609
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 4 cut(s) 72, 203, 404, 519
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 3 cut(s) 312, 328, 379
ScrFI CCNGG 1 cut(s) 459
SduI GDGCHC 1 cut(s) 512
SetI ASST 3 cut(s) 258, 365, 460
SfaNI GCATC 4 cut(s) 21, 157, 270, 379
SinI GGWCC 1 cut(s) 379
SmlI CTYRAG 3 cut(s) 226, 490, 561
SmoI CTYRAG 3 cut(s) 226, 490, 561
SphI GCATGC 1 cut(s) 570
Sse9I AATT 4 cut(s) 35, 216, 335, 503
SsiI CCGC 2 cut(s) 74, 518
StyD4I CCNGG 1 cut(s) 457
TaaI ACNGT 4 cut(s) 53, 82, 475, 545
TaqI TCGA 3 cut(s) 48, 87, 247
TasI AATT 4 cut(s) 35, 216, 335, 503
TatI WGTACW 2 cut(s) 450, 580
TauI GCSGC 1 cut(s) 521
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TscAI CASTG 1 cut(s) 135
TseI GCWGC 3 cut(s) 71, 202, 403
TspRI CASTG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 379
XceI RCATGY 2 cut(s) 174, 570
Zsp2I ATGCAT 2 cut(s) 6, 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.