FvH4_2g00850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001195
Physical Location & Seq
Reverse (-)
886705 .. 888587
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00850.t1

Sequence Viewer

Length: 633 bp
ATGGCTGGACCCGACGGACATGTAAGAAAGCTAGCCCTCTTCTTTGAGAAACTCGGCGCCTCTTCTCCTCCGGATACGAGAAAAAGTTCAGAAATCAGGTCCAACCCCGAAGGACCGGTGGCTGGTGGAGGTGCTTCTGTGGACAAACGACAATTTGTGCCTTGCGATGTGTGTTCTAAGTTGAACCCCCCTAATCTTAGTTGCTCTTACTCTGTGGGGCCTTGTGATATTTGTGACAAGGTTCAACGCATTAATTGTTGCCCTTATCTCAACCACCTCCCACAGGGGGCAACTTTTAACTCTGGATATGGAATAGTTTGTGTATGTGGTTATGTATTTGATGAGGTTAAGTGGGTTGTCCCTTATGTGGTGGGAGCGGAGCAGTGCTTAAGGCCAAGAATTGTTCCATTTGTAACGTCTGGAAGCAGCACTGTGCATATGAATGCCCAAAAAGACAAGGATCTTGCTGCCGAGTATAAATCGATCAGAGAAAAGATGCTTTCAGAAGTGTCGACTCCCATTGCATGGTCAGGTACCTATGTTCCAACTGATCCTTGTGGAGACATGAAAGAAAAAGATAATCAAACACGCCGGGGGAAATTTTTAATAGCTGCAGTGTTTCAATATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

22.93

Weight (kDa)

8.45

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 533
AccB1I GGYRCC 2 cut(s) 56, 533
AccB7I CCANNNNNTGG 1 cut(s) 525
AccBSI CCGCTC 1 cut(s) 377
AccI GTMKAC 1 cut(s) 512
AccIII TCCGGA 1 cut(s) 70
AciI CCGC 1 cut(s) 377
AclWI GGATC 2 cut(s) 468, 545
AcsI RAATTY 1 cut(s) 599
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 535
AfiI CCNNNNNNNGG 5 cut(s) 122, 283, 286, 367, 525
AflII CTTAAG 1 cut(s) 388
AflIII ACRYGT 1 cut(s) 19
AgeI ACCGGT 1 cut(s) 115
AgsI TTSAA 3 cut(s) 184, 245, 623
AluBI AGCT 2 cut(s) 31, 611
AluI AGCT 2 cut(s) 31, 611
Alw26I GTCTC 1 cut(s) 555
AlwI GGATC 2 cut(s) 468, 545
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 2 cut(s) 218, 392
ApeKI GCWGC 3 cut(s) 426, 467, 611
ApoI RAATTY 1 cut(s) 599
AseI ATTAAT 1 cut(s) 252
AsiGI ACCGGT 1 cut(s) 115
Asp700I GAANNNNTTC 1 cut(s) 85
Asp718I GGTACC 1 cut(s) 533
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 4 cut(s) 8, 99, 113, 218
AsuC2I CCSGG 1 cut(s) 593
AsuNHI GCTAGC 1 cut(s) 31
AvaII GGWCC 3 cut(s) 8, 99, 113
BaeI ACNNNNGTAYC 2 cut(s) 525, 558
BanI GGYRCC 2 cut(s) 56, 533
BbvI GCAGC 3 cut(s) 438, 454, 598
BciVI GTATCC 1 cut(s) 67
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 612
BfoI RGCGCY 1 cut(s) 60
BfrI CTTAAG 1 cut(s) 388
BfuI GTATCC 1 cut(s) 67
BisI GCNGC 3 cut(s) 427, 468, 612
BlsI GCNGC 3 cut(s) 428, 469, 613
Bme1390I CCNGG 1 cut(s) 593
Bme18I GGWCC 3 cut(s) 8, 99, 113
BmgT120I GGNCC 4 cut(s) 8, 99, 113, 218
BmiI GGNNCC 4 cut(s) 10, 58, 219, 535
BmrFI CCNGG 1 cut(s) 593
BmsI GCATC 1 cut(s) 486
BmtI GCTAGC 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 593
Bsa29I ATCGAT 1 cut(s) 482
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 592
BsaWI WCCGGW 2 cut(s) 70, 115
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 283, 286, 367, 525
Bse118I RCCGGY 1 cut(s) 115
Bse3DI GCAATG 1 cut(s) 519
BseAI TCCGGA 1 cut(s) 70
BseCI ATCGAT 1 cut(s) 482
BseDI CCNNGG 1 cut(s) 592
BseLI CCNNNNNNNGG 5 cut(s) 122, 283, 286, 367, 525
BseMI GCAATG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 3 cut(s) 438, 454, 598
BshFI GGCC 2 cut(s) 220, 394
BshNI GGYRCC 2 cut(s) 56, 533
BshTI ACCGGT 1 cut(s) 115
BshVI ATCGAT 1 cut(s) 482
BsiSI CCGG 3 cut(s) 71, 116, 592
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 5 cut(s) 122, 283, 286, 367, 525
BsmAI GTCTC 1 cut(s) 555
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 2 cut(s) 220, 394
Bsp13I TCCGGA 1 cut(s) 70
Bsp143I GATC 3 cut(s) 460, 483, 550
BspACI CCGC 1 cut(s) 377
BspANI GGCC 2 cut(s) 220, 394
BspDI ATCGAT 1 cut(s) 482
BspEI TCCGGA 1 cut(s) 70
BspLI GGNNCC 4 cut(s) 10, 58, 219, 535
BspMAI CTGCAG 1 cut(s) 616
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 2 cut(s) 468, 545
BspT107I GGYRCC 2 cut(s) 56, 533
BspTI CTTAAG 1 cut(s) 388
BsrBI CCGCTC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 1 cut(s) 115
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 1 cut(s) 592
BssMI GATC 3 cut(s) 460, 483, 550
BssNI GRCGYC 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 2 cut(s) 44, 67
BstACI GRCGYC 1 cut(s) 57
BstAFI CTTAAG 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 2 cut(s) 177, 197
BstENI CCTNNNNNAGG 1 cut(s) 281
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 3 cut(s) 463, 486, 553
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 3 cut(s) 460, 483, 550
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 591
BstSFI CTRYAG 1 cut(s) 612
BstV1I GCAGC 3 cut(s) 438, 454, 598
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
Bsu15I ATCGAT 1 cut(s) 482
BsuI GTATCC 1 cut(s) 67
BsuRI GGCC 2 cut(s) 220, 394
BsuTUI ATCGAT 1 cut(s) 482
BtgZI GCGATG 1 cut(s) 180
BtsI GCAGTG 2 cut(s) 389, 621
BtsIMutI CAGTG 3 cut(s) 389, 429, 621
Cac8I GCNNGC 1 cut(s) 33
CfoI GCGC 1 cut(s) 59
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 4 cut(s) 8, 99, 113, 218
ClaI ATCGAT 1 cut(s) 482
Csp6I GTAC 1 cut(s) 534
CspAI ACCGGT 1 cut(s) 115
CviAII CATG 3 cut(s) 20, 525, 565
CviJI RGCY 7 cut(s) 5, 31, 35, 122, 220, 394, 611
CviKI_1 RGCY 7 cut(s) 5, 31, 35, 122, 220, 394, 611
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 2 cut(s) 177, 197
DinI GGCGCC 1 cut(s) 58
DpnI GATC 3 cut(s) 462, 485, 552
DpnII GATC 3 cut(s) 460, 483, 550
Eam1104I CTCTTC 2 cut(s) 44, 67
EarI CTCTTC 2 cut(s) 44, 67
Eco47I GGWCC 3 cut(s) 8, 99, 113
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 218
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
FaeI CATG 3 cut(s) 23, 528, 568
FaqI GGGAC 1 cut(s) 344
FatI CATG 3 cut(s) 19, 524, 564
FauNDI CATATG 1 cut(s) 438
FblI GTMKAC 1 cut(s) 512
Fnu4HI GCNGC 3 cut(s) 427, 468, 612
Fsp4HI GCNGC 3 cut(s) 427, 468, 612
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 58
GluI GCNGC 3 cut(s) 427, 468, 612
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 2 cut(s) 220, 394
HapII CCGG 3 cut(s) 71, 116, 592
HhaI GCGC 1 cut(s) 59
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 3 cut(s) 23, 528, 568
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HinfI GANTC 1 cut(s) 514
HpaII CCGG 3 cut(s) 71, 116, 592
Hpy166II GTNNAC 2 cut(s) 142, 513
Hpy188I TCNGA 3 cut(s) 91, 488, 505
Hpy188III TCNNGA 3 cut(s) 71, 303, 420
Hpy8I GTNNAC 2 cut(s) 142, 513
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 3 cut(s) 436, 524, 614
HpyF3I CTNAG 2 cut(s) 177, 197
HpySE526I ACGT 1 cut(s) 416
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 3 cut(s) 23, 528, 568
HspAI GCGC 1 cut(s) 57
KasI GGCGCC 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 70
KpnI GGTACC 1 cut(s) 537
Kzo9I GATC 3 cut(s) 460, 483, 550
LmnI GCTCC 2 cut(s) 374, 379
LpnPI CCDG 9 cut(s) 82, 84, 108, 129, 269, 288, 405, 516, 605
Lsp1109I GCAGC 3 cut(s) 438, 454, 598
LweI GCATC 1 cut(s) 486
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 2 cut(s) 233, 412
MalI GATC 3 cut(s) 462, 485, 552
MbiI CCGCTC 1 cut(s) 377
MboI GATC 3 cut(s) 460, 483, 550
MboII GAAGA 2 cut(s) 31, 54
MflI RGATCY 1 cut(s) 460
MluCI AATT 4 cut(s) 152, 253, 399, 599
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 2 cut(s) 126, 569
MnlI CCTC 6 cut(s) 47, 70, 78, 122, 287, 337
MroI TCCGGA 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 85
MseI TTAA 5 cut(s) 252, 297, 348, 389, 605
MslI CAYNNNNRTG 1 cut(s) 441
MspCI CTTAAG 1 cut(s) 388
MspI CCGG 3 cut(s) 71, 116, 592
MspR9I CCNGG 1 cut(s) 593
Mva1269I GAATGC 1 cut(s) 448
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 1 cut(s) 593
NdeI CATATG 1 cut(s) 438
NdeII GATC 3 cut(s) 460, 483, 550
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 3 cut(s) 23, 528, 568
NlaIV GGNNCC 4 cut(s) 10, 58, 219, 535
NmeAIII GCCGAG 2 cut(s) 33, 496
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 1 cut(s) 23
PciI ACATGT 1 cut(s) 19
PctI GAATGC 1 cut(s) 448
PdmI GAANNNNTTC 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 525
PinAI ACCGGT 1 cut(s) 115
PkrI GCNGC 3 cut(s) 428, 469, 613
PleI GAGTC 1 cut(s) 508
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 508
PscI ACATGT 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 252
PspN4I GGNNCC 4 cut(s) 10, 58, 219, 535
PspPI GGNCC 4 cut(s) 8, 99, 113, 218
PstI CTGCAG 1 cut(s) 616
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
RseI CAYNNNNRTG 1 cut(s) 441
SalI GTCGAC 1 cut(s) 511
SaqAI TTAA 5 cut(s) 252, 297, 348, 389, 605
SatI GCNGC 3 cut(s) 427, 468, 612
Sau3AI GATC 3 cut(s) 460, 483, 550
Sau96I GGNCC 4 cut(s) 8, 99, 113, 218
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 1 cut(s) 593
SfaNI GCATC 1 cut(s) 486
SfcI CTRYAG 1 cut(s) 612
SfoI GGCGCC 1 cut(s) 58
SinI GGWCC 3 cut(s) 8, 99, 113
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
Sse9I AATT 4 cut(s) 152, 253, 399, 599
SsiI CCGC 1 cut(s) 377
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 591
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 419
TaqI TCGA 2 cut(s) 482, 512
TasI AATT 4 cut(s) 152, 253, 399, 599
Tru1I TTAA 5 cut(s) 252, 297, 348, 389, 605
Tru9I TTAA 5 cut(s) 252, 297, 348, 389, 605
TscAI CASTG 3 cut(s) 389, 436, 621
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 3 cut(s) 426, 467, 611
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 455, 581
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 3 cut(s) 389, 436, 621
Van91I CCANNNNNTGG 1 cut(s) 525
Vha464I CTTAAG 1 cut(s) 388
VpaK11BI GGWCC 3 cut(s) 8, 99, 113
VspI ATTAAT 1 cut(s) 252
XagI CCTNNNNNAGG 1 cut(s) 281
XapI RAATTY 1 cut(s) 599
XceI RCATGY 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 512
XmnI GAANNNNTTC 1 cut(s) 85
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.