Rw5G035970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001195
Physical Location & Seq
Reverse (-)
62823674 .. 62825097
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035970.1

Sequence Viewer

Length: 747 bp
ATGGCTGGACCCGACGCAAGCTGTATGGATGAGATCTGCCAGGGAGATGAAACTGTTTCAAAAGGCGTAAGAAACCTAGCCATCTTCTTTGAGACTCTCGGCGCCTCTTCTCCCGATACAAGGAAACAGATCAGTTCCAGGGCCCGGGCACCTAGTTCTAAAACCAGCAATCGAAAAGGTTGCGGAGGATCCACTGGTTCAGGTTTTCCCTTAGGCACCTATACCTATACTCCTGTCGACTTCAGGTCCAAGTTCAGAAAACCAGTGACTGGTGGAGATGCTTCTATGGACAAAGCAGTGCCTTGCAATGAGTGTTCTAAGGAGCACCCACCTTATTCTTTTTGCCCTTACGCGCCTTGTCATATTTGTTATAAGGTTCACCACCATAGTTATTGCCCTTACTTTGACCATATCCCACAGGGTGCAACTTTTGACTCTGGCTATCAAATAATTTGTGGATGTGGTAGTGAATTTAATGAGGATAAGTGGGTTTGTACCTCTTGTGGTGGGAGCAGAGCAATGCTTAAGGCCAAGTATTGTAACATTTGTAACAGTGTGAAACAGCACCGCGCATATGAATGCCCGAAGGACAAGGTTCTTGCCGCCAAGTATAAATTGGCCAGGGACAAGAGGTGTTCAGTTTTGCCAAATAGGATTCCCTATGACATTGCCTATATACCAGAATCATCATCAGAAAAGATCTATGTTCCAACTCCTCCTCCTGGGTGGGTTTGTGGACACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

248

Amino Acids

27.23

Weight (kDa)

8.66

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccB1I GGYRCC 3 cut(s) 101, 148, 215
AccB7I CCANNNNNTGG 1 cut(s) 269
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 353, 570
AciI CCGC 3 cut(s) 183, 568, 603
AclWI GGATC 2 cut(s) 183, 196
AcoI YGGCCR 1 cut(s) 618
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 422
AfaI GTAC 1 cut(s) 496
AfiI CCNNNNNNNGG 4 cut(s) 120, 144, 269, 722
AflII CTTAAG 1 cut(s) 524
AgsI TTSAA 1 cut(s) 60
AhdI GACNNNNNGTC 1 cut(s) 244
AjnI CCWGG 4 cut(s) 39, 137, 620, 721
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 2 cut(s) 183, 196
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 3 cut(s) 141, 528, 618
ApaI GGGCCC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 470
AspLEI GCGC 3 cut(s) 104, 355, 572
AspS9I GGNCC 4 cut(s) 8, 141, 142, 246
AsuC2I CCSGG 2 cut(s) 145, 146
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 8, 246
AxyI CCTNAGG 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 145, 151
BalI TGGCCA 1 cut(s) 620
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 3 cut(s) 101, 148, 215
BanII GRGCYC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 327
BccI CCATC 1 cut(s) 89
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 4 cut(s) 41, 139, 622, 723
BcnI CCSGG 2 cut(s) 145, 146
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 2 cut(s) 77, 153
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 524
BglII AGATCT 2 cut(s) 33, 699
BisI GCNGC 1 cut(s) 603
BlsI GCNGC 1 cut(s) 604
Bme1390I CCNGG 6 cut(s) 41, 139, 145, 146, 622, 723
Bme18I GGWCC 2 cut(s) 8, 246
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 4 cut(s) 8, 141, 142, 246
BmiI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 217
BmrFI CCNGG 6 cut(s) 41, 139, 145, 146, 622, 723
BmsI GCATC 1 cut(s) 268
BpuMI CCSGG 2 cut(s) 145, 146
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 5 cut(s) 40, 138, 144, 621, 722
BsaXI ACNNNNNCTCC 4 cut(s) 214, 244, 703, 733
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 144, 269, 722
Bse1I ACTGG 3 cut(s) 199, 263, 274
Bse21I CCTNAGG 1 cut(s) 211
Bse3DI GCAATG 3 cut(s) 313, 525, 666
BseBI CCWGG 4 cut(s) 41, 139, 622, 723
BseDI CCNNGG 5 cut(s) 40, 138, 144, 621, 722
BseGI GGATG 2 cut(s) 34, 464
BseLI CCNNNNNNNGG 4 cut(s) 120, 144, 269, 722
BseMI GCAATG 3 cut(s) 313, 525, 666
BseNI ACTGG 3 cut(s) 199, 263, 274
BseRI GAGGAG 2 cut(s) 705, 708
BseSI GKGCMC 2 cut(s) 145, 151
Bsh1236I CGCG 2 cut(s) 353, 570
BshFI GGCC 3 cut(s) 143, 530, 620
BshNI GGYRCC 3 cut(s) 101, 148, 215
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 4 cut(s) 120, 144, 269, 722
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 638
BsmI GAATGC 1 cut(s) 584
BsnI GGCC 3 cut(s) 143, 530, 620
BsoBI CYCGRG 1 cut(s) 144
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 3 cut(s) 145, 151, 327
Bsp143I GATC 4 cut(s) 33, 129, 188, 699
BspACI CCGC 3 cut(s) 183, 568, 603
BspANI GGCC 3 cut(s) 143, 530, 620
BspFNI CGCG 2 cut(s) 353, 570
BspLI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 217
BspPI GGATC 2 cut(s) 183, 196
BspT107I GGYRCC 3 cut(s) 101, 148, 215
BspTI CTTAAG 1 cut(s) 524
BsrDI GCAATG 3 cut(s) 313, 525, 666
BsrI ACTGG 3 cut(s) 199, 263, 274
BssECI CCNNGG 5 cut(s) 40, 138, 144, 621, 722
BssMI GATC 4 cut(s) 33, 129, 188, 699
BssNI GRCGYC 1 cut(s) 102
Bst2UI CCWGG 4 cut(s) 41, 139, 622, 723
Bst4CI ACNGT 2 cut(s) 55, 554
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 211, 318
BstF5I GGATG 2 cut(s) 34, 464
BstFNI CGCG 2 cut(s) 353, 570
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 3 cut(s) 104, 355, 572
BstKTI GATC 4 cut(s) 36, 132, 191, 702
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 4 cut(s) 33, 129, 188, 699
BstNI CCWGG 4 cut(s) 41, 139, 622, 723
BstSCI CCNGG 6 cut(s) 39, 137, 143, 144, 620, 721
BstSLI GKGCMC 2 cut(s) 145, 151
BstUI CGCG 2 cut(s) 353, 570
BstX2I RGATCY 3 cut(s) 33, 188, 699
BstYI RGATCY 3 cut(s) 33, 188, 699
Bsu36I CCTNAGG 1 cut(s) 211
BsuRI GGCC 3 cut(s) 143, 530, 620
BtsCI GGATG 2 cut(s) 34, 464
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 4 cut(s) 192, 270, 303, 559
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 269
CfoI GCGC 3 cut(s) 104, 355, 572
Cfr13I GGNCC 4 cut(s) 8, 141, 142, 246
Cfr9I CCCGGG 1 cut(s) 144
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 495
CviAII CATG 1 cut(s) 744
CviJI RGCY 7 cut(s) 5, 21, 80, 143, 441, 530, 620
CviKI_1 RGCY 7 cut(s) 5, 21, 80, 143, 441, 530, 620
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 2 cut(s) 211, 318
DinI GGCGCC 1 cut(s) 103
DpnI GATC 4 cut(s) 35, 131, 190, 701
DpnII GATC 4 cut(s) 33, 129, 188, 699
DraIII CACNNNGTG 1 cut(s) 422
DriI GACNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 618
Eam1104I CTCTTC 1 cut(s) 112
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 8, 246
Eco57I CTGAAG 1 cut(s) 226
Eco81I CCTNAGG 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 141
EcoRII CCWGG 4 cut(s) 39, 137, 620, 721
EcoT38I GRGCYC 1 cut(s) 145
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
FaeI CATG 1 cut(s) 747
FaqI GGGAC 1 cut(s) 638
FatI CATG 1 cut(s) 743
FauNDI CATATG 1 cut(s) 574
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 1 cut(s) 603
FokI GGATG 2 cut(s) 41, 471
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 1 cut(s) 603
FspBI CTAG 2 cut(s) 77, 153
GlaI GCGC 3 cut(s) 103, 354, 571
GluI GCNGC 1 cut(s) 603
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 3 cut(s) 143, 530, 620
HapII CCGG 1 cut(s) 145
HgaI GACGC 1 cut(s) 23
HhaI GCGC 3 cut(s) 104, 355, 572
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 1 cut(s) 747
Hin6I GCGC 3 cut(s) 102, 353, 570
HinP1I GCGC 3 cut(s) 102, 353, 570
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 4 cut(s) 94, 434, 655, 683
HpaII CCGG 1 cut(s) 145
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 3 cut(s) 238, 379, 737
Hpy188I TCNGA 2 cut(s) 257, 694
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 3 cut(s) 238, 379, 737
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 580
HpyCH4III ACNGT 2 cut(s) 55, 554
HpyCH4V TGCA 2 cut(s) 306, 425
HpyF3I CTNAG 2 cut(s) 211, 318
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 1 cut(s) 747
HspAI GCGC 3 cut(s) 102, 353, 570
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 4 cut(s) 33, 129, 188, 699
LmnI GCTCC 2 cut(s) 322, 510
LweI GCATC 1 cut(s) 268
MaeI CTAG 2 cut(s) 77, 153
MaeIII GTNAC 3 cut(s) 265, 539, 548
MalI GATC 4 cut(s) 35, 131, 190, 701
MboI GATC 4 cut(s) 33, 129, 188, 699
MboII GAAGA 2 cut(s) 76, 99
MflI RGATCY 3 cut(s) 33, 188, 699
MhlI GDGCHC 3 cut(s) 145, 151, 327
MlsI TGGCCA 1 cut(s) 620
MluCI AATT 3 cut(s) 450, 470, 614
MluNI TGGCCA 1 cut(s) 620
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 2 cut(s) 88, 428
MmeI TCCRAC 1 cut(s) 734
MnlI CCTC 7 cut(s) 115, 179, 472, 508, 624, 726, 729
Mox20I TGGCCA 1 cut(s) 620
MscI TGGCCA 1 cut(s) 620
MseI TTAA 2 cut(s) 474, 525
MslI CAYNNNNRTG 1 cut(s) 577
Msp20I TGGCCA 1 cut(s) 620
MspCI CTTAAG 1 cut(s) 524
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 6 cut(s) 41, 139, 145, 146, 622, 723
Mva1269I GAATGC 1 cut(s) 584
MvaI CCWGG 4 cut(s) 41, 139, 622, 723
MvnI CGCG 2 cut(s) 353, 570
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 2 cut(s) 145, 146
NdeI CATATG 1 cut(s) 574
NdeII GATC 4 cut(s) 33, 129, 188, 699
NlaIII CATG 1 cut(s) 747
NlaIV GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 217
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 265
PctI GAATGC 1 cut(s) 584
PfeI GAWTC 2 cut(s) 655, 683
PflMI CCANNNNNTGG 1 cut(s) 269
PkrI GCNGC 1 cut(s) 604
PleI GAGTC 2 cut(s) 88, 428
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 2 cut(s) 88, 428
PsiI TTATAA 1 cut(s) 372
Psp6I CCWGG 4 cut(s) 39, 137, 620, 721
PspGI CCWGG 4 cut(s) 39, 137, 620, 721
PspN4I GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 217
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 4 cut(s) 8, 141, 142, 246
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 3 cut(s) 33, 188, 699
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
RseI CAYNNNNRTG 1 cut(s) 577
SalI GTCGAC 1 cut(s) 236
SaqAI TTAA 2 cut(s) 474, 525
SatI GCNGC 1 cut(s) 603
Sau3AI GATC 4 cut(s) 33, 129, 188, 699
Sau96I GGNCC 4 cut(s) 8, 141, 142, 246
SchI GAGTC 2 cut(s) 88, 428
ScrFI CCNGG 6 cut(s) 41, 139, 145, 146, 622, 723
SduI GDGCHC 3 cut(s) 145, 151, 327
SfaNI GCATC 1 cut(s) 268
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 2 cut(s) 8, 246
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 577
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
SrfI GCCCGGGC 1 cut(s) 146
Sse9I AATT 3 cut(s) 450, 470, 614
SsiI CCGC 3 cut(s) 183, 568, 603
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 77, 153
StyD4I CCNGG 6 cut(s) 39, 137, 143, 144, 620, 721
TaaI ACNGT 2 cut(s) 55, 554
TaqI TCGA 2 cut(s) 172, 237
TasI AATT 3 cut(s) 450, 470, 614
TauI GCSGC 1 cut(s) 605
TfiI GAWTC 2 cut(s) 655, 683
Tru1I TTAA 2 cut(s) 474, 525
Tru9I TTAA 2 cut(s) 474, 525
TscAI CASTG 4 cut(s) 199, 270, 303, 559
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 63, 591
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 4 cut(s) 199, 270, 303, 559
Van91I CCANNNNNTGG 1 cut(s) 269
Vha464I CTTAAG 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 8, 246
XapI RAATTY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 2 cut(s) 77, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.