Rorug06G0201600

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001195
Physical Location & Seq
Reverse (-)
32362816 .. 32365685
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0201600.1

Sequence Viewer

Length: 726 bp
ATGACGTCGTCACCGGCGGCCAAACTCCCTTGGCTTAAATCGAGCCTTCACTTTCCAGCACCAACGGTAAGATCGAGGACCTCAAAGTGGAGGGTCCGAAGATTAGAATCGGAGCTCTGGTCGACGGGGGAGGTGAAATCTGCTTTGGTCAAGAGCAGCGGAGGCGCTCATGTGAATACTGAAAAGAAAGACAAAGGTCGAGCAATGGCATCCCGGTCCCAGATTGTTGGTTATCCCTGCATAGGACCCGAGAGAGCTCAAGTTTGCTTTGGAATCATTCTTGGAGTTGACATTGATCTCGTAAATTTTGCATTCTTCAGTTATGAGAGCAGTGTGATTCTCATGGCAAATTTCAGCAAAGCAAATGAAAGCAAGTGCAAAGCTCACAAAATGAGCTACCGTACCATTGAAGCTACATGCCTTGCAGTAAACATGAAAGTTATGATTTGCGCTATGAGGGGTTCTCTTCGTCTCTCTCGAATCCACATTCATTTCATACTAATACAGCTTTTAACATCAATTGGAAACTTCTGCACCTATTCAATTGTAATTGTTGTTGGAATGGTTATTGGGATATCAGTCGGAACTCAGAGAAGGGCTGCCCGTTCTTACTGGTGCCAACACCAAGCTAAGCAACATGGCAAGTATCAGGGATGTGTCCCAAGGATTATAGACAGTAGCTCGACTTGTAGACATGGAGCTGCAAGGTATTCGGCCACTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

26.65

Weight (kDa)

10.04

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 615
AccI GTMKAC 2 cut(s) 122, 691
AciI CCGC 2 cut(s) 17, 159
AcoI YGGCCR 2 cut(s) 18, 714
AcsI RAATTY 2 cut(s) 304, 349
AcuI CTGAAG 1 cut(s) 301
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 403
AfiI CCNNNNNNNGG 2 cut(s) 87, 242
AgsI TTSAA 2 cut(s) 410, 543
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 9 cut(s) 115, 257, 383, 396, 413, 508, 629, 681, 701
AluI AGCT 9 cut(s) 115, 257, 383, 396, 413, 508, 629, 681, 701
Alw21I GWGCWC 2 cut(s) 117, 259
Alw26I GTCTC 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 2 cut(s) 18, 714
ApeKI GCWGC 3 cut(s) 156, 599, 701
ApoI RAATTY 2 cut(s) 304, 349
AspLEI GCGC 2 cut(s) 167, 452
AspS9I GGNCC 4 cut(s) 78, 94, 216, 245
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 2 cut(s) 3, 145
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 4 cut(s) 78, 94, 216, 245
BanI GGYRCC 1 cut(s) 615
BanII GRGCYC 2 cut(s) 117, 259
Bbv12I GWGCWC 2 cut(s) 117, 259
BbvI GCAGC 3 cut(s) 168, 586, 688
BcgI CGANNNNNNTGC 1 cut(s) 693
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 476
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 4 cut(s) 18, 157, 600, 702
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 4 cut(s) 19, 158, 601, 703
Bme1390I CCNGG 1 cut(s) 214
Bme18I GGWCC 4 cut(s) 78, 94, 216, 245
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 4 cut(s) 78, 94, 216, 245
BmiI GGNNCC 4 cut(s) 95, 218, 247, 617
BmrFI CCNGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 218
BoxI GACNNNNGTC 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 448, 480
Bpu1102I GCTNAGC 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 243
BpuMI CCSGG 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 29, 662
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 242
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 1 cut(s) 617
Bse3DI GCAATG 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 106
BseDI CCNNGG 2 cut(s) 29, 662
BseGI GGATG 2 cut(s) 209, 659
BseJI GATNNNNATC 1 cut(s) 106
BseLI CCNNNNNNNGG 2 cut(s) 87, 242
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 1 cut(s) 617
BseXI GCAGC 3 cut(s) 168, 586, 688
BsgI GTGCAG 1 cut(s) 517
BshFI GGCC 2 cut(s) 20, 716
BshNI GGYRCC 1 cut(s) 615
BsiHKAI GWGCWC 2 cut(s) 117, 259
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 2 cut(s) 14, 214
BslFI GGGAC 2 cut(s) 202, 644
BslI CCNNNNNNNGG 2 cut(s) 87, 242
BsmAI GTCTC 1 cut(s) 476
BsmBI CGTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 202, 644
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 2 cut(s) 20, 716
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 117, 259
Bsp143I GATC 2 cut(s) 71, 295
Bsp1720I GCTNAGC 1 cut(s) 630
BspACI CCGC 2 cut(s) 17, 159
BspANI GGCC 2 cut(s) 20, 716
BspCNI CTCAG 1 cut(s) 601
BspLI GGNNCC 4 cut(s) 95, 218, 247, 617
BspT107I GGYRCC 1 cut(s) 615
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 1 cut(s) 617
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 29, 662
BssMI GATC 2 cut(s) 71, 295
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 29, 662
Bst4CI ACNGT 4 cut(s) 67, 401, 677, 721
Bst6I CTCTTC 1 cut(s) 471
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 2 cut(s) 588, 630
BstF5I GGATG 2 cut(s) 209, 659
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 2 cut(s) 167, 452
BstKTI GATC 2 cut(s) 74, 298
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 2 cut(s) 71, 295
BstMWI GCNNNNNNNGC 1 cut(s) 162
BstNSI RCATGY 1 cut(s) 420
BstPAI GACNNNNGTC 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 212
BstV1I GCAGC 3 cut(s) 168, 586, 688
BstXI CCANNNNNNTGG 1 cut(s) 227
BsuRI GGCC 2 cut(s) 20, 716
BtsCI GGATG 2 cut(s) 209, 659
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 337, 717
CfoI GCGC 2 cut(s) 167, 452
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 4 cut(s) 78, 94, 216, 245
Csp6I GTAC 1 cut(s) 402
CviAII CATG 6 cut(s) 170, 343, 417, 433, 638, 695
CviQI GTAC 1 cut(s) 402
DdeI CTNAG 2 cut(s) 588, 630
DpnI GATC 2 cut(s) 73, 297
DpnII GATC 2 cut(s) 71, 295
EaeI YGGCCR 2 cut(s) 18, 714
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Ecl136II GAGCTC 2 cut(s) 115, 257
Eco130I CCWWGG 2 cut(s) 29, 662
Eco24I GRGCYC 2 cut(s) 117, 259
Eco32I GATATC 1 cut(s) 576
Eco47I GGWCC 4 cut(s) 78, 94, 216, 245
Eco53kI GAGCTC 2 cut(s) 115, 257
Eco57I CTGAAG 1 cut(s) 301
Eco88I CYCGRG 1 cut(s) 248
EcoICRI GAGCTC 2 cut(s) 115, 257
EcoO109I RGGNCCY 2 cut(s) 78, 245
EcoRV GATATC 1 cut(s) 576
EcoT14I CCWWGG 2 cut(s) 29, 662
EcoT38I GRGCYC 2 cut(s) 117, 259
ErhI CCWWGG 2 cut(s) 29, 662
Esp3I CGTCTC 1 cut(s) 476
FaeI CATG 6 cut(s) 173, 346, 420, 436, 641, 698
FaqI GGGAC 2 cut(s) 202, 644
FatI CATG 6 cut(s) 169, 342, 416, 432, 637, 694
FblI GTMKAC 2 cut(s) 122, 691
Fnu4HI GCNGC 4 cut(s) 18, 157, 600, 702
FokI GGATG 2 cut(s) 196, 666
FriOI GRGCYC 2 cut(s) 117, 259
Fsp4HI GCNGC 4 cut(s) 18, 157, 600, 702
GlaI GCGC 2 cut(s) 166, 451
GluI GCNGC 4 cut(s) 18, 157, 600, 702
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 2 cut(s) 20, 716
HapII CCGG 2 cut(s) 14, 214
HhaI GCGC 2 cut(s) 167, 452
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 173, 346, 420, 436, 641, 698
Hin6I GCGC 2 cut(s) 165, 450
HinP1I GCGC 2 cut(s) 165, 450
HincII GTYRAC 2 cut(s) 123, 289
HindII GTYRAC 2 cut(s) 123, 289
HinfI GANTC 4 cut(s) 107, 273, 337, 480
HpaII CCGG 2 cut(s) 14, 214
HphI GGTGA 2 cut(s) 3, 145
Hpy166II GTNNAC 4 cut(s) 123, 289, 430, 692
Hpy188I TCNGA 4 cut(s) 98, 112, 584, 591
Hpy188III TCNNGA 2 cut(s) 151, 477
Hpy8I GTNNAC 4 cut(s) 123, 289, 430, 692
Hpy99I CGWCG 2 cut(s) 10, 127
HpyAV CCTTC 2 cut(s) 56, 588
HpyCH4III ACNGT 4 cut(s) 67, 401, 677, 721
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 6 cut(s) 240, 311, 378, 425, 534, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 162
HpyF3I CTNAG 2 cut(s) 588, 630
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 173, 346, 420, 436, 641, 698
HspAI GCGC 2 cut(s) 165, 450
Kzo9I GATC 2 cut(s) 71, 295
LmnI GCTCC 2 cut(s) 112, 698
LpnPI CCDG 8 cut(s) 27, 69, 103, 227, 233, 250, 598, 635
Lsp1109I GCAGC 3 cut(s) 168, 586, 688
LweI GCATC 1 cut(s) 218
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 9
MalI GATC 2 cut(s) 73, 297
MboI GATC 2 cut(s) 71, 295
MboII GAAGA 3 cut(s) 111, 307, 458
MfeI CAATTG 2 cut(s) 519, 543
MhlI GDGCHC 2 cut(s) 117, 259
MluCI AATT 5 cut(s) 304, 349, 519, 543, 549
MmeI TCCRAC 2 cut(s) 538, 562
MnlI CCTC 6 cut(s) 69, 84, 91, 124, 155, 450
MseI TTAA 2 cut(s) 36, 512
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 2 cut(s) 14, 214
MspR9I CCNGG 1 cut(s) 214
MunI CAATTG 2 cut(s) 519, 543
Mva1269I GAATGC 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 162
NciI CCSGG 1 cut(s) 214
NdeII GATC 2 cut(s) 71, 295
NlaIII CATG 6 cut(s) 173, 346, 420, 436, 641, 698
NlaIV GGNNCC 4 cut(s) 95, 218, 247, 617
NmuCI GTSAC 1 cut(s) 9
NspI RCATGY 1 cut(s) 420
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 475
PctI GAATGC 1 cut(s) 311
PfeI GAWTC 4 cut(s) 107, 273, 337, 480
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 4 cut(s) 19, 158, 601, 703
PpuMI RGGWCCY 2 cut(s) 78, 245
PshAI GACNNNNGTC 1 cut(s) 195
Psp124BI GAGCTC 2 cut(s) 117, 259
Psp5II RGGWCCY 2 cut(s) 78, 245
PspN4I GGNNCC 4 cut(s) 95, 218, 247, 617
PspPI GGNCC 4 cut(s) 78, 94, 216, 245
PspPPI RGGWCCY 2 cut(s) 78, 245
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 1 cut(s) 403
RsaNI GTAC 1 cut(s) 402
SacI GAGCTC 2 cut(s) 117, 259
SalI GTCGAC 1 cut(s) 121
SaqAI TTAA 2 cut(s) 36, 512
SatI GCNGC 4 cut(s) 18, 157, 600, 702
Sau3AI GATC 2 cut(s) 71, 295
Sau96I GGNCC 4 cut(s) 78, 94, 216, 245
ScrFI CCNGG 1 cut(s) 214
SduI GDGCHC 2 cut(s) 117, 259
SfaNI GCATC 1 cut(s) 218
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 4 cut(s) 78, 94, 216, 245
SmlI CTYRAG 1 cut(s) 258
SmoI CTYRAG 1 cut(s) 258
Sse9I AATT 5 cut(s) 304, 349, 519, 543, 549
SsiI CCGC 2 cut(s) 17, 159
SstI GAGCTC 2 cut(s) 117, 259
StyD4I CCNGG 1 cut(s) 212
StyI CCWWGG 2 cut(s) 29, 662
TaaI ACNGT 4 cut(s) 67, 401, 677, 721
TaiI ACGT 1 cut(s) 8
TaqI TCGA 6 cut(s) 41, 74, 122, 199, 478, 683
TasI AATT 5 cut(s) 304, 349, 519, 543, 549
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 4 cut(s) 107, 273, 337, 480
Tru1I TTAA 2 cut(s) 36, 512
Tru9I TTAA 2 cut(s) 36, 512
TscAI CASTG 2 cut(s) 337, 724
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 3 cut(s) 156, 599, 701
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 4 cut(s) 381, 449, 479, 484
TspRI CASTG 2 cut(s) 337, 724
Tth111I GACNNNGTC 1 cut(s) 7
VpaK11BI GGWCC 4 cut(s) 78, 94, 216, 245
XapI RAATTY 2 cut(s) 304, 349
XceI RCATGY 1 cut(s) 420
XmiI GTMKAC 2 cut(s) 122, 691
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.