Rmu_sc0000367.1_g000136

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000367.1
OG0001195
Physical Location & Seq
Reverse (-)
669833 .. 671262
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000367.1_g000136.1.cds

Sequence Viewer

Length: 768 bp
atggctggacccgacgcaagctgtgtggatgagatctgccagggagatgaaactgtttcaaaaggcgtaagaaacctagccatcttctttgagactctcggcgcctcttctcccgatacaaggaaacagatcagttccagggcccgggcacctagttctaaaaccagcaatcgaaaaggttgcggaggatccactggttcaggttttgccttaggtattgggtactgcaaacaagcaggcacctatacctatactcctgtcgacttcaggtccaagttcagaaaaccagtgactggtggagatgcttctatggacaaagcagtgccttgcaatgagtgttctaaggagcaccccccttattctttttgcccttacgcgccttgtcatatttgttataaggttcaccaccatagttattgcccttactttgaccatatcccacagggtgcaacttttgactctggctatcaaataatttgtggatgtggtagtgaatttaatgaggataagtgggtttgtacctcttgtggtgggagcagagcaatgcttaaggccaagtattgtaacatttgtaacagtgtgaaacagcaccgcgcatatgaatgcccgaaggacaaggttcttgccgccaagtataaattggccagggacaagaggtgttcagttgtgccaaataggattccctatgacattgcctatataccagaatcatcatcagaaaagatctatgttccaactcctcctgggtgggtttgtggacacacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

27.89

Weight (kDa)

8.7

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
AccB1I GGYRCC 3 cut(s) 101, 148, 239
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 1 cut(s) 261
AccII CGCG 2 cut(s) 377, 594
AciI CCGC 3 cut(s) 183, 592, 627
AclWI GGATC 2 cut(s) 183, 196
AcoI YGGCCR 1 cut(s) 642
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 250
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 2 cut(s) 224, 520
AfiI CCNNNNNNNGG 3 cut(s) 120, 144, 293
AflII CTTAAG 1 cut(s) 548
AgsI TTSAA 1 cut(s) 60
AhdI GACNNNNNGTC 1 cut(s) 268
AjnI CCWGG 4 cut(s) 39, 137, 644, 742
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 2 cut(s) 183, 196
AlwNI CAGNNNCTG 1 cut(s) 293
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 3 cut(s) 141, 552, 642
ApaI GGGCCC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 494
AspLEI GCGC 3 cut(s) 104, 379, 596
AspS9I GGNCC 4 cut(s) 8, 141, 142, 270
AsuC2I CCSGG 2 cut(s) 145, 146
AsuHPI GGTGA 1 cut(s) 395
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 8, 270
AxyI CCTNAGG 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 145, 151
BalI TGGCCA 1 cut(s) 644
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 3 cut(s) 101, 148, 239
BanII GRGCYC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 351
BccI CCATC 1 cut(s) 89
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 4 cut(s) 41, 139, 646, 744
BcnI CCSGG 2 cut(s) 145, 146
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 2 cut(s) 77, 153
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 548
BglII AGATCT 2 cut(s) 33, 723
BisI GCNGC 1 cut(s) 627
BlsI GCNGC 1 cut(s) 628
Bme1390I CCNGG 6 cut(s) 41, 139, 145, 146, 646, 744
Bme18I GGWCC 2 cut(s) 8, 270
BmeRI GACNNNNNGTC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 4 cut(s) 8, 141, 142, 270
BmiI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 241
BmrFI CCNGG 6 cut(s) 41, 139, 145, 146, 646, 744
BmsI GCATC 1 cut(s) 292
BpuMI CCSGG 2 cut(s) 145, 146
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 5 cut(s) 40, 138, 144, 645, 743
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 144, 293
Bse1I ACTGG 3 cut(s) 199, 287, 298
Bse21I CCTNAGG 1 cut(s) 211
Bse3DI GCAATG 3 cut(s) 337, 549, 690
BseBI CCWGG 4 cut(s) 41, 139, 646, 744
BseDI CCNNGG 5 cut(s) 40, 138, 144, 645, 743
BseGI GGATG 2 cut(s) 34, 488
BseLI CCNNNNNNNGG 3 cut(s) 120, 144, 293
BseMI GCAATG 3 cut(s) 337, 549, 690
BseNI ACTGG 3 cut(s) 199, 287, 298
BseRI GAGGAG 1 cut(s) 729
BseSI GKGCMC 2 cut(s) 145, 151
Bsh1236I CGCG 2 cut(s) 377, 594
BshFI GGCC 3 cut(s) 143, 554, 644
BshNI GGYRCC 3 cut(s) 101, 148, 239
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 3 cut(s) 120, 144, 293
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 662
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 3 cut(s) 143, 554, 644
BsoBI CYCGRG 1 cut(s) 144
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 3 cut(s) 145, 151, 351
Bsp143I GATC 4 cut(s) 33, 129, 188, 723
BspACI CCGC 3 cut(s) 183, 592, 627
BspANI GGCC 3 cut(s) 143, 554, 644
BspFNI CGCG 2 cut(s) 377, 594
BspLI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 241
BspPI GGATC 2 cut(s) 183, 196
BspT107I GGYRCC 3 cut(s) 101, 148, 239
BspTI CTTAAG 1 cut(s) 548
BsrDI GCAATG 3 cut(s) 337, 549, 690
BsrI ACTGG 3 cut(s) 199, 287, 298
BssECI CCNNGG 5 cut(s) 40, 138, 144, 645, 743
BssMI GATC 4 cut(s) 33, 129, 188, 723
BssNI GRCGYC 1 cut(s) 102
Bst2UI CCWGG 4 cut(s) 41, 139, 646, 744
Bst4CI ACNGT 2 cut(s) 55, 578
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 19, 238
BstDEI CTNAG 2 cut(s) 211, 342
BstF5I GGATG 2 cut(s) 34, 488
BstFNI CGCG 2 cut(s) 377, 594
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 3 cut(s) 104, 379, 596
BstKTI GATC 4 cut(s) 36, 132, 191, 726
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 4 cut(s) 33, 129, 188, 723
BstNI CCWGG 4 cut(s) 41, 139, 646, 744
BstSCI CCNGG 6 cut(s) 39, 137, 143, 144, 644, 742
BstSLI GKGCMC 2 cut(s) 145, 151
BstUI CGCG 2 cut(s) 377, 594
BstX2I RGATCY 3 cut(s) 33, 188, 723
BstYI RGATCY 3 cut(s) 33, 188, 723
Bsu36I CCTNAGG 1 cut(s) 211
BsuRI GGCC 3 cut(s) 143, 554, 644
BtsCI GGATG 2 cut(s) 34, 488
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 4 cut(s) 192, 294, 327, 583
Cac8I GCNNGC 2 cut(s) 19, 238
CaiI CAGNNNCTG 1 cut(s) 293
CfoI GCGC 3 cut(s) 104, 379, 596
Cfr13I GGNCC 4 cut(s) 8, 141, 142, 270
Cfr9I CCCGGG 1 cut(s) 144
CseI GACGC 1 cut(s) 23
Csp6I GTAC 2 cut(s) 223, 519
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 1 cut(s) 765
CviJI RGCY 7 cut(s) 5, 21, 80, 143, 465, 554, 644
CviKI_1 RGCY 7 cut(s) 5, 21, 80, 143, 465, 554, 644
CviQI GTAC 2 cut(s) 223, 519
DdeI CTNAG 2 cut(s) 211, 342
DinI GGCGCC 1 cut(s) 103
DpnI GATC 4 cut(s) 35, 131, 190, 725
DpnII GATC 4 cut(s) 33, 129, 188, 723
DraIII CACNNNGTG 1 cut(s) 446
DriI GACNNNNNGTC 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 112
Eam1105I GACNNNNNGTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 8, 270
Eco57I CTGAAG 1 cut(s) 250
Eco81I CCTNAGG 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 141
EcoRII CCWGG 4 cut(s) 39, 137, 644, 742
EcoT38I GRGCYC 1 cut(s) 145
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
FaeI CATG 1 cut(s) 768
FaqI GGGAC 1 cut(s) 662
FatI CATG 1 cut(s) 764
FauNDI CATATG 1 cut(s) 598
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 1 cut(s) 627
FokI GGATG 2 cut(s) 41, 495
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 1 cut(s) 627
FspBI CTAG 2 cut(s) 77, 153
GlaI GCGC 3 cut(s) 103, 378, 595
GluI GCNGC 1 cut(s) 627
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 3 cut(s) 143, 554, 644
HapII CCGG 1 cut(s) 145
HgaI GACGC 1 cut(s) 23
HhaI GCGC 3 cut(s) 104, 379, 596
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 1 cut(s) 768
Hin6I GCGC 3 cut(s) 102, 377, 594
HinP1I GCGC 3 cut(s) 102, 377, 594
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 4 cut(s) 94, 458, 679, 707
HpaII CCGG 1 cut(s) 145
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 3 cut(s) 262, 403, 758
Hpy188I TCNGA 2 cut(s) 281, 718
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 3 cut(s) 262, 403, 758
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 604
HpyCH4III ACNGT 2 cut(s) 55, 578
HpyCH4V TGCA 3 cut(s) 228, 330, 449
HpyF3I CTNAG 2 cut(s) 211, 342
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 1 cut(s) 768
HspAI GCGC 3 cut(s) 102, 377, 594
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 4 cut(s) 33, 129, 188, 723
LmnI GCTCC 2 cut(s) 346, 534
LweI GCATC 1 cut(s) 292
MaeI CTAG 2 cut(s) 77, 153
MaeIII GTNAC 3 cut(s) 289, 563, 572
MalI GATC 4 cut(s) 35, 131, 190, 725
MboI GATC 4 cut(s) 33, 129, 188, 723
MboII GAAGA 2 cut(s) 76, 99
MflI RGATCY 3 cut(s) 33, 188, 723
MhlI GDGCHC 3 cut(s) 145, 151, 351
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 3 cut(s) 474, 494, 638
MluNI TGGCCA 1 cut(s) 644
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 2 cut(s) 88, 452
MmeI TCCRAC 1 cut(s) 758
MnlI CCTC 6 cut(s) 115, 179, 496, 532, 648, 750
Mox20I TGGCCA 1 cut(s) 644
MscI TGGCCA 1 cut(s) 644
MseI TTAA 2 cut(s) 498, 549
MslI CAYNNNNRTG 1 cut(s) 601
Msp20I TGGCCA 1 cut(s) 644
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 6 cut(s) 41, 139, 145, 146, 646, 744
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 4 cut(s) 41, 139, 646, 744
MvnI CGCG 2 cut(s) 377, 594
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 2 cut(s) 145, 146
NdeI CATATG 1 cut(s) 598
NdeII GATC 4 cut(s) 33, 129, 188, 723
NlaIII CATG 1 cut(s) 768
NlaIV GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 241
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 289
PctI GAATGC 1 cut(s) 608
PfeI GAWTC 2 cut(s) 679, 707
PflMI CCANNNNNTGG 1 cut(s) 293
PkrI GCNGC 1 cut(s) 628
PleI GAGTC 2 cut(s) 88, 452
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 2 cut(s) 88, 452
PsiI TTATAA 1 cut(s) 396
Psp6I CCWGG 4 cut(s) 39, 137, 644, 742
PspGI CCWGG 4 cut(s) 39, 137, 644, 742
PspN4I GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 241
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 4 cut(s) 8, 141, 142, 270
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 3 cut(s) 33, 188, 723
RsaI GTAC 2 cut(s) 224, 520
RsaNI GTAC 2 cut(s) 223, 519
RseI CAYNNNNRTG 1 cut(s) 601
SalI GTCGAC 1 cut(s) 260
SaqAI TTAA 2 cut(s) 498, 549
SatI GCNGC 1 cut(s) 627
Sau3AI GATC 4 cut(s) 33, 129, 188, 723
Sau96I GGNCC 4 cut(s) 8, 141, 142, 270
SchI GAGTC 2 cut(s) 88, 452
ScrFI CCNGG 6 cut(s) 41, 139, 145, 146, 646, 744
SduI GDGCHC 3 cut(s) 145, 151, 351
SfaNI GCATC 1 cut(s) 292
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 2 cut(s) 8, 270
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SrfI GCCCGGGC 1 cut(s) 146
Sse9I AATT 3 cut(s) 474, 494, 638
SsiI CCGC 3 cut(s) 183, 592, 627
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 77, 153
StyD4I CCNGG 6 cut(s) 39, 137, 143, 144, 644, 742
TaaI ACNGT 2 cut(s) 55, 578
TaqI TCGA 2 cut(s) 172, 261
TasI AATT 3 cut(s) 474, 494, 638
TauI GCSGC 1 cut(s) 629
TfiI GAWTC 2 cut(s) 679, 707
Tru1I TTAA 2 cut(s) 498, 549
Tru9I TTAA 2 cut(s) 498, 549
TscAI CASTG 4 cut(s) 199, 294, 327, 583
TseFI GTSAC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 2 cut(s) 63, 615
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 4 cut(s) 199, 294, 327, 583
Van91I CCANNNNNTGG 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 548
VpaK11BI GGWCC 2 cut(s) 8, 270
XapI RAATTY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 637
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 2 cut(s) 77, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.