RLG00000012322

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001195
Physical Location & Seq
Reverse (-)
17477135 .. 17478459
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012322

Sequence Viewer

Length: 432 bp
ATGATCCAAAAAAAAGCAAAGGCTGCTAGAGTACCTTCTTTGGATATAGATTTGTCTACCATCGTCATATTGAGACCATCGGCAGCCGGCAAGGAGAAGATCATCCAGAGCTGCGAATCTGAAGAAAAAGCAAAGGCCACCACCGTGCCAAAGGACGATACTCATGATCACTGGCGGATCCAGCGTGCTCGAATCTGTAAAGCGGATGTGTTCTGGAACGGCGACGACTACAAGACTTGTAATATTTGTTCTGAGGAGCACTTAATGGGTTCTCTCTACCCTTACTGGGAATGTGTTCCGCCGGGCACAACTGTCGACCCTCAATATCATGTAATTTGTGTGGGATGTGGTCGTATGATTCTCGATTGGTCTGAAACTTGGATTGCCCAGTCGACAAGGATAGAGCTGCCAGGTTCAAGGACCTTCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

16.19

Weight (kDa)

6.41

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 56, 315, 392
AciI CCGC 3 cut(s) 175, 203, 299
AclWI GGATC 2 cut(s) 172, 185
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 286
AgsI TTSAA 1 cut(s) 417
AjnI CCWGG 1 cut(s) 409
AleI CACNNNNGTG 1 cut(s) 143
AluBI AGCT 2 cut(s) 111, 406
AluI AGCT 2 cut(s) 111, 406
Alw21I GWGCWC 2 cut(s) 190, 261
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 2 cut(s) 172, 185
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 4 cut(s) 23, 83, 111, 406
Asp700I GAANNNNTTC 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 420
AsuC2I CCSGG 1 cut(s) 303
AvaII GGWCC 1 cut(s) 420
BaeGI GKGCMC 1 cut(s) 308
BamHI GGATCC 1 cut(s) 177
Bbv12I GWGCWC 2 cut(s) 190, 261
BbvI GCAGC 4 cut(s) 10, 95, 98, 393
BccI CCATC 2 cut(s) 68, 85
BceAI ACGGC 1 cut(s) 235
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 1 cut(s) 411
BclI TGATCA 1 cut(s) 166
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 1 cut(s) 27
BisI GCNGC 4 cut(s) 24, 84, 112, 407
BlsI GCNGC 4 cut(s) 25, 85, 113, 408
Bme1390I CCNGG 2 cut(s) 303, 411
Bme18I GGWCC 1 cut(s) 420
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 1 cut(s) 179
BmrFI CCNGG 2 cut(s) 303, 411
BmrI ACTGGG 2 cut(s) 295, 382
BmuI ACTGGG 2 cut(s) 295, 382
BpuMI CCSGG 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 67
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 3 cut(s) 176, 290, 388
BseBI CCWGG 1 cut(s) 411
BseGI GGATG 3 cut(s) 102, 211, 350
BseLI CCNNNNNNNGG 1 cut(s) 286
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 3 cut(s) 176, 290, 388
BseRI GAGGAG 1 cut(s) 269
BseSI GKGCMC 1 cut(s) 308
BseXI GCAGC 4 cut(s) 10, 95, 98, 393
BshFI GGCC 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 190, 261
BsiSI CCGG 2 cut(s) 87, 302
BslI CCNNNNNNNGG 1 cut(s) 286
BsmAI GTCTC 1 cut(s) 67
BsnI GGCC 1 cut(s) 137
Bso31I GGTCTC 1 cut(s) 67
Bsp1286I GDGCHC 3 cut(s) 190, 261, 308
Bsp143I GATC 4 cut(s) 3, 99, 166, 177
BspACI CCGC 3 cut(s) 175, 203, 299
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 1 cut(s) 244
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 179
BspPI GGATC 2 cut(s) 172, 185
BspTNI GGTCTC 1 cut(s) 67
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 3 cut(s) 176, 290, 388
BssAI RCCGGY 1 cut(s) 86
BssMI GATC 4 cut(s) 3, 99, 166, 177
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 145, 313
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 88, 186
BstDEI CTNAG 1 cut(s) 252
BstF5I GGATG 3 cut(s) 102, 211, 350
BstKTI GATC 4 cut(s) 6, 102, 169, 180
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 4 cut(s) 3, 99, 166, 177
BstMWI GCNNNNNNNGC 2 cut(s) 23, 181
BstNI CCWGG 1 cut(s) 411
BstSCI CCNGG 2 cut(s) 301, 409
BstSLI GKGCMC 1 cut(s) 308
BstV1I GCAGC 4 cut(s) 10, 95, 98, 393
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BsuRI GGCC 1 cut(s) 137
BtsCI GGATG 3 cut(s) 102, 211, 350
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 88, 186
CciI TCATGA 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 420
Csp6I GTAC 1 cut(s) 32
CviAII CATG 2 cut(s) 164, 329
CviJI RGCY 5 cut(s) 23, 86, 111, 137, 406
CviKI_1 RGCY 5 cut(s) 23, 86, 111, 137, 406
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 1 cut(s) 252
DpnI GATC 4 cut(s) 5, 101, 168, 179
DpnII GATC 4 cut(s) 3, 99, 166, 177
EciI GGCGGA 2 cut(s) 190, 288
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 420
Eco57I CTGAAG 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 409
FaeI CATG 2 cut(s) 167, 332
FaiI YATR 6 cut(s) 47, 68, 165, 330, 356, 430
FatI CATG 2 cut(s) 163, 328
FbaI TGATCA 1 cut(s) 166
FblI GTMKAC 3 cut(s) 56, 315, 392
Fnu4HI GCNGC 4 cut(s) 24, 84, 112, 407
FokI GGATG 3 cut(s) 89, 218, 357
Fsp4HI GCNGC 4 cut(s) 24, 84, 112, 407
FspBI CTAG 1 cut(s) 27
GluI GCNGC 4 cut(s) 24, 84, 112, 407
HaeIII GGCC 1 cut(s) 137
HapII CCGG 2 cut(s) 87, 302
Hin1II CATG 2 cut(s) 167, 332
HincII GTYRAC 2 cut(s) 316, 393
HindII GTYRAC 2 cut(s) 316, 393
HinfI GANTC 3 cut(s) 116, 192, 358
HpaII CCGG 2 cut(s) 87, 302
Hpy166II GTNNAC 3 cut(s) 57, 316, 393
Hpy188I TCNGA 3 cut(s) 121, 253, 373
Hpy188III TCNNGA 4 cut(s) 106, 164, 214, 362
Hpy8I GTNNAC 3 cut(s) 57, 316, 393
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 45
HpyCH4III ACNGT 2 cut(s) 145, 313
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 181
HpyF3I CTNAG 1 cut(s) 252
Hsp92II CATG 2 cut(s) 167, 332
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 4 cut(s) 3, 99, 166, 177
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 4 cut(s) 10, 95, 98, 393
MaeI CTAG 1 cut(s) 27
MalI GATC 4 cut(s) 5, 101, 168, 179
MboI GATC 4 cut(s) 3, 99, 166, 177
MboII GAAGA 2 cut(s) 109, 134
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 3 cut(s) 190, 261, 308
MluCI AATT 1 cut(s) 333
MnlI CCTC 2 cut(s) 247, 330
MroNI GCCGGC 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 1 cut(s) 263
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 2 cut(s) 87, 302
MspR9I CCNGG 2 cut(s) 303, 411
MvaI CCWGG 1 cut(s) 411
MwoI GCNNNNNNNGC 2 cut(s) 23, 181
NaeI GCCGGC 1 cut(s) 88
NciI CCSGG 1 cut(s) 303
NdeII GATC 4 cut(s) 3, 99, 166, 177
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 2 cut(s) 167, 332
NlaIV GGNNCC 1 cut(s) 179
OliI CACNNNNGTG 1 cut(s) 143
PagI TCATGA 1 cut(s) 163
PdiI GCCGGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 3 cut(s) 116, 192, 358
PkrI GCNGC 4 cut(s) 25, 85, 113, 408
PpuMI RGGWCCY 1 cut(s) 420
Psp5II RGGWCCY 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 179
PspPI GGNCC 1 cut(s) 420
PspPPI RGGWCCY 1 cut(s) 420
PsrI GAACNNNNNNTAC 2 cut(s) 232, 264
PsuI RGATCY 1 cut(s) 177
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 143
SalI GTCGAC 2 cut(s) 314, 391
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 4 cut(s) 24, 84, 112, 407
Sau3AI GATC 4 cut(s) 3, 99, 166, 177
Sau96I GGNCC 1 cut(s) 420
ScrFI CCNGG 2 cut(s) 303, 411
SduI GDGCHC 3 cut(s) 190, 261, 308
SetI ASST 5 cut(s) 37, 113, 408, 415, 425
SinI GGWCC 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 143
Sse9I AATT 1 cut(s) 333
SsiI CCGC 3 cut(s) 175, 203, 299
SspI AATATT 1 cut(s) 244
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 301, 409
TaaI ACNGT 2 cut(s) 145, 313
TaqI TCGA 4 cut(s) 190, 315, 363, 392
TasI AATT 1 cut(s) 333
TfiI GAWTC 3 cut(s) 116, 192, 358
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TscAI CASTG 1 cut(s) 176
TseI GCWGC 4 cut(s) 23, 83, 111, 406
TspRI CASTG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 420
XmiI GTMKAC 3 cut(s) 56, 315, 392
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.