RLG00000035233

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001195
Physical Location & Seq
Reverse (-)
63271823 .. 63273443
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035233

Sequence Viewer

Length: 765 bp
ATGGCTGGACCCGACGCAAGCTGTGTGGATGAGATCTGCCAGGGAGATGAAATGGTTTCAAAAGGCGTAAGAAACCTAGCCGTCTTCTTTGAGACTCTAGGCGCCTCTTCTCCGGATACAAGGAAACAGTTCAGTTCCAGGGTCCGGGCACCTAGTTCTAAAACCAGCAATCGAAAAGGTTGCGGAGCATCCGCTGGTTCAGGCACCTATACCTATACTCCTGTCGACTTCAGTTCCAAGTTCAGAGGACCAGTGGCTGGTGGAGGCCCTTCTATGGACAAAGCAGTGCCTTGCAATGAGTGTTCTAAGGAGCACCCTCCTTATTCTTATTGCCCTTACGCGCCTTGTCATATTTGTTATAAGGTTCACCACCCTAGTTATTGCCCTTACTTTGACCATATCCCACAGGGTGCAACTTTTCACCATGGCTATGAAATAATTTGTGGATGTGGTAATGAATTTAATGAGGATAAGTGGGTTTGTACCTCTTGTGGTGGGAGCAGAGCAGTGCTTAAGGCCAAGTGTTGTAAAATTTGTAATAGCGTGAAACAGCATGGCGCATATGAATGCCCGAAGGACAAGGTTCTTGCTGCCGAGTATAAATTGGCCAGAGACAAGAGGCGTTCAGTTGTGCCGAATTGGATTCCCTATAATACTGTTTGTTTTCCAAAATCATCATCAGGAAAGATCTATGTTCCAACTCCTCCTCCTGGGTGGGTTTGTGGACACACGAAAGAAGGCAAGCTTACTGGGTCTACGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

27.59

Weight (kDa)

8.84

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AccB1I GGYRCC 3 cut(s) 101, 148, 203
AccB7I CCANNNNNTGG 1 cut(s) 257
AccI GTMKAC 2 cut(s) 225, 755
AccII CGCG 1 cut(s) 341
AccIII TCCGGA 1 cut(s) 112
AciI CCGC 2 cut(s) 183, 192
AcoI YGGCCR 1 cut(s) 606
AcsI RAATTY 2 cut(s) 458, 531
AcuI CTGAAG 1 cut(s) 214
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 4 cut(s) 144, 257, 274, 710
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 1 cut(s) 60
AjnI CCWGG 3 cut(s) 39, 137, 709
AluBI AGCT 2 cut(s) 21, 745
AluI AGCT 2 cut(s) 21, 745
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 86, 606
AlwNI CAGNNNCTG 1 cut(s) 257
Aor13HI TCCGGA 1 cut(s) 112
AoxI GGCC 3 cut(s) 265, 516, 606
ApeKI GCWGC 1 cut(s) 590
ApoI RAATTY 2 cut(s) 458, 531
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 3 cut(s) 104, 343, 560
AspS9I GGNCC 4 cut(s) 8, 142, 248, 266
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 2 cut(s) 359, 413
AvaII GGWCC 3 cut(s) 8, 142, 248
BaeGI GKGCMC 1 cut(s) 151
BalI TGGCCA 1 cut(s) 608
BanI GGYRCC 3 cut(s) 101, 148, 203
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 577
BceAI ACGGC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 3 cut(s) 41, 139, 711
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 2 cut(s) 86, 606
BfaI CTAG 4 cut(s) 77, 98, 153, 375
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 512
BfuI GTATCC 1 cut(s) 109
BglII AGATCT 2 cut(s) 33, 687
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
Bme1390I CCNGG 4 cut(s) 41, 139, 146, 711
Bme18I GGWCC 3 cut(s) 8, 142, 248
BmgT120I GGNCC 4 cut(s) 8, 142, 248, 266
BmiI GGNNCC 5 cut(s) 10, 103, 143, 150, 205
BmrFI CCNGG 4 cut(s) 41, 139, 146, 711
BmrI ACTGGG 1 cut(s) 759
BmsI GCATC 1 cut(s) 197
BmuI ACTGGG 1 cut(s) 759
BpiI GAAGAC 1 cut(s) 76
BpuMI CCSGG 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 40, 138, 424, 710
BsaWI WCCGGW 1 cut(s) 112
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 691, 721
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 257, 274, 710
Bse1I ACTGG 2 cut(s) 251, 754
Bse3DI GCAATG 1 cut(s) 301
BseAI TCCGGA 1 cut(s) 112
BseBI CCWGG 3 cut(s) 41, 139, 711
BseDI CCNNGG 4 cut(s) 40, 138, 424, 710
BseGI GGATG 3 cut(s) 34, 188, 452
BseLI CCNNNNNNNGG 4 cut(s) 144, 257, 274, 710
BseMI GCAATG 1 cut(s) 301
BseNI ACTGG 2 cut(s) 251, 754
BseRI GAGGAG 2 cut(s) 693, 696
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 1 cut(s) 577
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 3 cut(s) 267, 518, 608
BshNI GGYRCC 3 cut(s) 101, 148, 203
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 2 cut(s) 113, 145
BslI CCNNNNNNNGG 4 cut(s) 144, 257, 274, 710
BsmAI GTCTC 2 cut(s) 86, 606
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 3 cut(s) 267, 518, 608
Bsp1286I GDGCHC 2 cut(s) 151, 315
Bsp13I TCCGGA 1 cut(s) 112
Bsp143I GATC 2 cut(s) 33, 687
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 2 cut(s) 183, 192
BspANI GGCC 3 cut(s) 267, 518, 608
BspEI TCCGGA 1 cut(s) 112
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 5 cut(s) 10, 103, 143, 150, 205
BspT107I GGYRCC 3 cut(s) 101, 148, 203
BspTI CTTAAG 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 2 cut(s) 251, 754
BssECI CCNNGG 4 cut(s) 40, 138, 424, 710
BssMI GATC 2 cut(s) 33, 687
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 3 cut(s) 41, 139, 711
Bst4CI ACNGT 2 cut(s) 129, 658
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 512
BstC8I GCNNGC 2 cut(s) 19, 743
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 3 cut(s) 34, 188, 452
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 3 cut(s) 104, 343, 560
BstKTI GATC 2 cut(s) 36, 690
BstMAI GTCTC 2 cut(s) 86, 606
BstMBI GATC 2 cut(s) 33, 687
BstNI CCWGG 3 cut(s) 41, 139, 711
BstSCI CCNGG 4 cut(s) 39, 137, 144, 709
BstSLI GKGCMC 1 cut(s) 151
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 577
BstV2I GAAGAC 1 cut(s) 76
BstX2I RGATCY 2 cut(s) 33, 687
BstYI RGATCY 2 cut(s) 33, 687
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 3 cut(s) 267, 518, 608
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 3 cut(s) 34, 188, 452
BtsI GCAGTG 2 cut(s) 291, 513
BtsIMutI CAGTG 3 cut(s) 258, 291, 513
Cac8I GCNNGC 2 cut(s) 19, 743
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 3 cut(s) 104, 343, 560
Cfr13I GGNCC 4 cut(s) 8, 142, 248, 266
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 483
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 2 cut(s) 425, 554
CviJI RGCY 9 cut(s) 5, 21, 80, 257, 267, 429, 518, 608, 745
CviKI_1 RGCY 9 cut(s) 5, 21, 80, 257, 267, 429, 518, 608, 745
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 1 cut(s) 306
DinI GGCGCC 1 cut(s) 103
DpnI GATC 2 cut(s) 35, 689
DpnII GATC 2 cut(s) 33, 687
DraIII CACNNNGTG 1 cut(s) 410
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 424
Eco47I GGWCC 3 cut(s) 8, 142, 248
Eco57I CTGAAG 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 3 cut(s) 39, 137, 709
EcoT14I CCWWGG 1 cut(s) 424
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 2 cut(s) 428, 557
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FatI CATG 2 cut(s) 424, 553
FauNDI CATATG 1 cut(s) 562
FblI GTMKAC 2 cut(s) 225, 755
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 3 cut(s) 41, 175, 459
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 4 cut(s) 77, 98, 153, 375
GlaI GCGC 3 cut(s) 103, 342, 559
GluI GCNGC 1 cut(s) 591
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 3 cut(s) 267, 518, 608
HapII CCGG 2 cut(s) 113, 145
HgaI GACGC 1 cut(s) 23
HhaI GCGC 3 cut(s) 104, 343, 560
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 2 cut(s) 428, 557
Hin6I GCGC 3 cut(s) 102, 341, 558
HinP1I GCGC 3 cut(s) 102, 341, 558
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 2 cut(s) 94, 643
HpaII CCGG 2 cut(s) 113, 145
HphI GGTGA 2 cut(s) 359, 413
Hpy166II GTNNAC 4 cut(s) 226, 367, 725, 756
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 2 cut(s) 113, 681
Hpy8I GTNNAC 4 cut(s) 226, 367, 725, 756
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 279, 568, 731
HpyCH4III ACNGT 2 cut(s) 129, 658
HpyCH4V TGCA 2 cut(s) 294, 413
HpyF3I CTNAG 1 cut(s) 306
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 2 cut(s) 428, 557
HspAI GCGC 3 cut(s) 102, 341, 558
KasI GGCGCC 1 cut(s) 101
Kpn2I TCCGGA 1 cut(s) 112
Kzo9I GATC 2 cut(s) 33, 687
LmnI GCTCC 3 cut(s) 185, 310, 498
Lsp1109I GCAGC 1 cut(s) 577
LweI GCATC 1 cut(s) 197
MaeI CTAG 4 cut(s) 77, 98, 153, 375
MalI GATC 2 cut(s) 35, 689
MboI GATC 2 cut(s) 33, 687
MboII GAAGA 2 cut(s) 76, 99
MflI RGATCY 2 cut(s) 33, 687
MhlI GDGCHC 2 cut(s) 151, 315
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 5 cut(s) 438, 458, 531, 602, 637
MluNI TGGCCA 1 cut(s) 608
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 722
Mox20I TGGCCA 1 cut(s) 608
MroI TCCGGA 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 608
MseI TTAA 2 cut(s) 462, 513
MslI CAYNNNNRTG 2 cut(s) 429, 565
Msp20I TGGCCA 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 2 cut(s) 113, 145
MspR9I CCNGG 4 cut(s) 41, 139, 146, 711
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 3 cut(s) 41, 139, 711
MvnI CGCG 1 cut(s) 341
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 1 cut(s) 146
NcoI CCATGG 1 cut(s) 424
NdeI CATATG 1 cut(s) 562
NdeII GATC 2 cut(s) 33, 687
NlaIII CATG 2 cut(s) 428, 557
NlaIV GGNNCC 5 cut(s) 10, 103, 143, 150, 205
NmeAIII GCCGAG 1 cut(s) 619
PctI GAATGC 1 cut(s) 572
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 643
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 88
PsiI TTATAA 1 cut(s) 360
Psp6I CCWGG 3 cut(s) 39, 137, 709
PspGI CCWGG 3 cut(s) 39, 137, 709
PspN4I GGNNCC 5 cut(s) 10, 103, 143, 150, 205
PspPI GGNCC 4 cut(s) 8, 142, 248, 266
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 2 cut(s) 33, 687
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
RseI CAYNNNNRTG 2 cut(s) 429, 565
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 462, 513
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 2 cut(s) 33, 687
Sau96I GGNCC 4 cut(s) 8, 142, 248, 266
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 4 cut(s) 41, 139, 146, 711
SduI GDGCHC 2 cut(s) 151, 315
SfaNI GCATC 1 cut(s) 197
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 3 cut(s) 8, 142, 248
SmiMI CAYNNNNRTG 2 cut(s) 429, 565
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 5 cut(s) 438, 458, 531, 602, 637
SsiI CCGC 2 cut(s) 183, 192
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 4 cut(s) 77, 98, 153, 375
StyD4I CCNGG 4 cut(s) 39, 137, 144, 709
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 2 cut(s) 129, 658
TaqI TCGA 2 cut(s) 172, 225
TasI AATT 5 cut(s) 438, 458, 531, 602, 637
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 2 cut(s) 462, 513
Tru9I TTAA 2 cut(s) 462, 513
TscAI CASTG 3 cut(s) 258, 291, 513
TseI GCWGC 1 cut(s) 590
TspDTI ATGAA 4 cut(s) 63, 447, 471, 579
TspRI CASTG 3 cut(s) 258, 291, 513
Van91I CCANNNNNTGG 1 cut(s) 257
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 3 cut(s) 8, 142, 248
XapI RAATTY 2 cut(s) 458, 531
XmiI GTMKAC 2 cut(s) 225, 755
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 4 cut(s) 77, 98, 153, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.