FvH4_4g30800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001195
Physical Location & Seq
Forward (+)
30242967 .. 30243764
798 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30800.t1

Sequence Viewer

Length: 633 bp
ATGGGGCTGAATGAGGTGATAGGACCGGTGGCTGGTGGAGGTGCTTCTGTGGACAAACGACAATGTGTGCCCTACGATGAGTGTTCTAACTCGCACCCCCGTTTTTCTTGTTGCCCTTACACCCCTTGTGGTATTTGTGACAAGGTTCACCTCCGCTCTGCTCGTTGCCCTTACTTCTACCATCTCCCACAGGGAGCGACTTGTCCCCGTGGAGATGAAATAGTTTGTCGATGTGGTAATGCATTTAACGAGGAAAAGTGGGCTTGTAGCTCTTGTGGTGGGAGCATGCCAGTGCTTAAGGTCAAGTATTGTTCCATTTGTAATGTCTGGTATCAGCACTGTGTATATGAATGCCCAGATGATCAGGTTGCTGCTGAGTATAAATCGATCAGAGACAAGATGCTTTCACGTGTGCCGATTCCCCATCGCCCTGTCTATCCAGAATCATCATCATCAGGAATTCCCTATGTTCCAACTACTCCTTCCTCGTTGGTTTGTGGAGACATGATCAAAGAAGGCAAGGACTCCGTCTATCCAGAAGCATTGTCAGGAATGACCTATTCTCCAACTGCTCGTCCCGGGGTTTGTGAAGACATGAACATAGGCACACCTTGTTGGAAGCCTATTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

22.98

Weight (kDa)

6.5

Isoelectric Point (pI)

53.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 156
AciI CCGC 1 cut(s) 154
AcsI RAATTY 1 cut(s) 459
AcvI CACGTG 1 cut(s) 410
AfiI CCNNNNNNNGG 1 cut(s) 32
AflII CTTAAG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 409
AgeI ACCGGT 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 466, 498
AluBI AGCT 1 cut(s) 270
AluI AGCT 1 cut(s) 270
Alw26I GTCTC 2 cut(s) 387, 495
Ama87I CYCGRG 1 cut(s) 578
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 1 cut(s) 459
AsiGI ACCGGT 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 2 cut(s) 579, 580
AsuHPI GGTGA 2 cut(s) 28, 140
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 72
BbrPI CACGTG 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 358
BccI CCATC 2 cut(s) 189, 432
BclI TGATCA 2 cut(s) 361, 507
BcnI CCSGG 2 cut(s) 579, 580
BcoDI GTCTC 2 cut(s) 387, 495
BfrI CTTAAG 1 cut(s) 296
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
Bme1390I CCNGG 2 cut(s) 579, 580
Bme18I GGWCC 1 cut(s) 23
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 1 cut(s) 23
BmrFI CCNGG 2 cut(s) 579, 580
BmsI GCATC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 597
BpuMI CCSGG 2 cut(s) 579, 580
Bsa29I ATCGAT 1 cut(s) 386
BsaAI YACGTR 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 208, 578, 579
BsaWI WCCGGW 1 cut(s) 25
BsaXI ACNNNNNCTCC 2 cut(s) 547, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 290
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 208, 578, 579
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 290
BseSI GKGCMC 1 cut(s) 72
BseXI GCAGC 1 cut(s) 358
BshTI ACCGGT 1 cut(s) 25
BshVI ATCGAT 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 2 cut(s) 26, 579
BslFI GGGAC 2 cut(s) 189, 561
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 2 cut(s) 387, 495
BsmFI GGGAC 2 cut(s) 189, 561
BsmI GAATGC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 361, 387, 507
BspACI CCGC 1 cut(s) 154
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 386
BspTI CTTAAG 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 290
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 208, 578, 579
BssMI GATC 3 cut(s) 361, 387, 507
Bst4CI ACNGT 1 cut(s) 341
BstAFI CTTAAG 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 287
BstDEI CTNAG 1 cut(s) 375
BstDSI CCRYGG 1 cut(s) 208
BstKTI GATC 3 cut(s) 364, 390, 510
BstMAI GTCTC 2 cut(s) 387, 495
BstMBI GATC 3 cut(s) 361, 387, 507
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 2 cut(s) 577, 578
BstSLI GKGCMC 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 597
Bsu15I ATCGAT 1 cut(s) 386
BsuTUI ATCGAT 1 cut(s) 386
BtgI CCRYGG 1 cut(s) 208
BtgZI GCGATG 1 cut(s) 410
BtsIMutI CAGTG 2 cut(s) 297, 337
Cac8I GCNNGC 1 cut(s) 287
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 23
Cfr9I CCCGGG 1 cut(s) 578
ClaI ATCGAT 1 cut(s) 386
CspAI ACCGGT 1 cut(s) 25
CviAII CATG 3 cut(s) 286, 505, 595
CviJI RGCY 5 cut(s) 7, 32, 263, 270, 622
CviKI_1 RGCY 5 cut(s) 7, 32, 263, 270, 622
DdeI CTNAG 1 cut(s) 375
DpnI GATC 3 cut(s) 363, 389, 509
DpnII GATC 3 cut(s) 361, 387, 507
Eco47I GGWCC 1 cut(s) 23
Eco72I CACGTG 1 cut(s) 410
Eco88I CYCGRG 1 cut(s) 578
EcoRI GAATTC 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 244
FaeI CATG 3 cut(s) 289, 508, 598
FaiI YATR 8 cut(s) 287, 346, 348, 381, 468, 506, 596, 602
FaqI GGGAC 2 cut(s) 189, 561
FatI CATG 3 cut(s) 285, 504, 594
FbaI TGATCA 2 cut(s) 361, 507
Fnu4HI GCNGC 1 cut(s) 372
Fsp4HI GCNGC 1 cut(s) 372
GluI GCNGC 1 cut(s) 372
HapII CCGG 2 cut(s) 26, 579
Hin1II CATG 3 cut(s) 289, 508, 598
HinfI GANTC 3 cut(s) 418, 443, 524
HpaII CCGG 2 cut(s) 26, 579
HphI GGTGA 2 cut(s) 28, 140
Hpy166II GTNNAC 2 cut(s) 52, 148
Hpy188I TCNGA 1 cut(s) 392
Hpy188III TCNNGA 4 cut(s) 440, 456, 536, 549
Hpy8I GTNNAC 2 cut(s) 52, 148
HpyAV CCTTC 2 cut(s) 492, 509
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 1 cut(s) 242
HpyF3I CTNAG 1 cut(s) 375
HpySE526I ACGT 1 cut(s) 409
Hsp92II CATG 3 cut(s) 289, 508, 598
Ksp22I TGATCA 2 cut(s) 361, 507
Kzo9I GATC 3 cut(s) 361, 387, 507
LmnI GCTCC 2 cut(s) 194, 282
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 1 cut(s) 390
MaeII ACGT 1 cut(s) 409
MaeIII GTNAC 1 cut(s) 137
MalI GATC 3 cut(s) 363, 389, 509
MbiI CCGCTC 1 cut(s) 156
MboI GATC 3 cut(s) 361, 387, 507
MboII GAAGA 1 cut(s) 602
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 1 cut(s) 459
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 3 cut(s) 497, 590, 596
MnlI CCTC 5 cut(s) 7, 32, 161, 244, 496
Mph1103I ATGCAT 1 cut(s) 244
MseI TTAA 2 cut(s) 246, 297
MslI CAYNNNNRTG 1 cut(s) 290
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 2 cut(s) 26, 579
MspR9I CCNGG 2 cut(s) 579, 580
Mva1269I GAATGC 1 cut(s) 356
NciI CCSGG 2 cut(s) 579, 580
NdeII GATC 3 cut(s) 361, 387, 507
NlaIII CATG 3 cut(s) 289, 508, 598
NmuCI GTSAC 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 244
NspI RCATGY 1 cut(s) 289
PaeI GCATGC 1 cut(s) 289
PctI GAATGC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 418, 443
PflFI GACNNNGTC 1 cut(s) 527
PinAI ACCGGT 1 cut(s) 25
PkrI GCNGC 1 cut(s) 373
PleI GAGTC 1 cut(s) 518
PmaCI CACGTG 1 cut(s) 410
PmlI CACGTG 1 cut(s) 410
PpsI GAGTC 1 cut(s) 518
Ppu21I YACGTR 1 cut(s) 410
PspCI CACGTG 1 cut(s) 410
PspPI GGNCC 1 cut(s) 23
PsyI GACNNNGTC 1 cut(s) 527
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 2 cut(s) 246, 297
SatI GCNGC 1 cut(s) 372
Sau3AI GATC 3 cut(s) 361, 387, 507
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 2 cut(s) 579, 580
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 1 cut(s) 390
SinI GGWCC 1 cut(s) 23
SmaI CCCGGG 1 cut(s) 580
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SphI GCATGC 1 cut(s) 289
Sse9I AATT 1 cut(s) 459
SsiI CCGC 1 cut(s) 154
StyD4I CCNGG 2 cut(s) 577, 578
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 1 cut(s) 412
TaqI TCGA 2 cut(s) 229, 386
TasI AATT 1 cut(s) 459
TfiI GAWTC 2 cut(s) 418, 443
Tru1I TTAA 2 cut(s) 246, 297
Tru9I TTAA 2 cut(s) 246, 297
TscAI CASTG 2 cut(s) 297, 344
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 3 cut(s) 231, 363, 611
TspGWI ACGGA 1 cut(s) 517
TspMI CCCGGG 1 cut(s) 578
TspRI CASTG 2 cut(s) 297, 344
Tth111I GACNNNGTC 1 cut(s) 527
Vha464I CTTAAG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 23
XapI RAATTY 1 cut(s) 459
XceI RCATGY 1 cut(s) 289
XmaI CCCGGG 1 cut(s) 578
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.