FvH4_4g36141

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001195
Physical Location & Seq
Forward (+)
33216642 .. 33218566
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36141.t2

Sequence Viewer

Length: 849 bp
ATGGCTGCATCCGACGCGAGCTCTCAGGGAGATGTAAAAAAGTTAGCCCTCTCCTTTGAGACTCTCGACGCCTCTTCTCCTGATACAAAGAAACCGTTCAGTTCCAGTTCTGAATGCAGCGATGGAAAAGGCTACACAGCATCCGATGGTTCAGGTTCCTATACCCCCGTCCCATTCAGGTCAGAGTTCATGGGGCCTTACAATGAGGATTCTAAGGTGAACCGCGAACAAAAAGCCGCCTATGTTCCAATGGGGGGTGAAGATGATTTTTACAGAGACATGTCCCAGTTCGCATTGTCTCGCATTAATGAGGATGAGGGTTCTAAGGTGATGAACTGCAAACAAACAGCAACCGATATTCCTGATGTCTTGGGGTCTGTGATCAGAGGACCAGTGTCGGGCGAAGGTGCTTATAAACACAAAAGACCATGTGTGCCTTGCAATGAGTGTTCTAAGGAGCACCCTCCTTGTCCTTCTTGCCCTTACGTGCTTTGTAATATTTGTCACGACCGTCACTACCCTAATTATTGCCCTAGCGTGCCTTGTGAAGTTTGTTATCAGTATCTTCACCATACCTTTTATTGCCCTTACTTTCGCCTTATCCCACAGGGTGCAGCTTTTGACCGTGACAACTATGAAATATTTTGTTGCTGTGGTAATGAATTTAATGAGGAAAAGTGGGTTTGTACCTATTGTACTGGGAGAAGAGCACTGCTGAAGCGTAAGTATTGTTCCATTTGTGATAGTAGCTGGAAACAGCACAGCACCCATGAATGCCCCAAGGACAAGGTTCTTGCTGCCGAGTCTAAATCAATTAGAGACCCATTATCTTTGTCAGGGCAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

31.49

Weight (kDa)

5.92

Isoelectric Point (pI)

54.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AccII CGCG 2 cut(s) 17, 225
AciI CCGC 2 cut(s) 223, 237
AcsI RAATTY 1 cut(s) 662
AcuI CTGAAG 1 cut(s) 737
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 611
AfaI GTAC 2 cut(s) 688, 697
AfiI CCNNNNNNNGG 2 cut(s) 254, 398
AflIII ACRYGT 1 cut(s) 279
AluBI AGCT 3 cut(s) 21, 617, 750
AluI AGCT 3 cut(s) 21, 617, 750
Alw21I GWGCWC 3 cut(s) 23, 462, 712
Alw26I GTCTC 4 cut(s) 53, 270, 303, 813
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 4 cut(s) 5, 117, 614, 797
ApoI RAATTY 1 cut(s) 662
AseI ATTAAT 1 cut(s) 306
Asp700I GAANNNNTTC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 194, 389
AsuHPI GGTGA 4 cut(s) 229, 269, 340, 560
AvaII GGWCC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 23
Bbv12I GWGCWC 3 cut(s) 23, 462, 712
BbvI GCAGC 3 cut(s) 129, 626, 784
BccI CCATC 2 cut(s) 116, 140
BclI TGATCA 1 cut(s) 381
BcoDI GTCTC 4 cut(s) 53, 270, 303, 813
BfaI CTAG 1 cut(s) 534
BisI GCNGC 5 cut(s) 6, 118, 237, 615, 798
BlsI GCNGC 5 cut(s) 7, 119, 238, 616, 799
Bme18I GGWCC 1 cut(s) 389
BmgT120I GGNCC 2 cut(s) 194, 389
BmiI GGNNCC 2 cut(s) 157, 195
BmrI ACTGGG 2 cut(s) 280, 708
BmsI GCATC 2 cut(s) 17, 149
BmuI ACTGGG 2 cut(s) 280, 708
BoxI GACNNNNGTC 1 cut(s) 394
BsaAI YACGTR 1 cut(s) 487
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 813
BsaJI CCNNGG 1 cut(s) 780
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 398
Bse1I ACTGG 4 cut(s) 105, 286, 392, 703
Bse3DI GCAATG 1 cut(s) 448
BseDI CCNNGG 1 cut(s) 780
BseGI GGATG 3 cut(s) 8, 140, 319
BseLI CCNNNNNNNGG 2 cut(s) 254, 398
BseMI GCAATG 1 cut(s) 448
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 4 cut(s) 105, 286, 392, 703
BseXI GCAGC 3 cut(s) 129, 626, 784
BsgI GTGCAG 1 cut(s) 633
Bsh1236I CGCG 2 cut(s) 17, 225
Bsh1285I CGRYCG 1 cut(s) 511
BshFI GGCC 1 cut(s) 196
BsiEI CGRYCG 1 cut(s) 511
BsiHKAI GWGCWC 3 cut(s) 23, 462, 712
BslFI GGGAC 2 cut(s) 155, 268
BslI CCNNNNNNNGG 2 cut(s) 254, 398
BsmAI GTCTC 4 cut(s) 53, 270, 303, 813
BsmFI GGGAC 2 cut(s) 155, 268
BsmI GAATGC 2 cut(s) 119, 779
BsnI GGCC 1 cut(s) 196
Bso31I GGTCTC 1 cut(s) 813
Bsp1286I GDGCHC 3 cut(s) 23, 462, 712
Bsp143I GATC 1 cut(s) 381
BspACI CCGC 2 cut(s) 223, 237
BspANI GGCC 1 cut(s) 196
BspCNI CTCAG 1 cut(s) 37
BspFNI CGCG 2 cut(s) 17, 225
BspLI GGNNCC 2 cut(s) 157, 195
BspQI GCTCTTC 1 cut(s) 700
BspTNI GGTCTC 1 cut(s) 813
BsrDI GCAATG 1 cut(s) 448
BsrI ACTGG 4 cut(s) 105, 286, 392, 703
BssECI CCNNGG 1 cut(s) 780
BssMI GATC 1 cut(s) 381
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 780
Bst4CI ACNGT 3 cut(s) 96, 512, 626
Bst6I CTCTTC 2 cut(s) 79, 700
BstACI GRCGYC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 2 cut(s) 19, 539
BstDEI CTNAG 4 cut(s) 24, 213, 324, 453
BstF5I GGATG 3 cut(s) 8, 140, 319
BstFNI CGCG 2 cut(s) 17, 225
BstKTI GATC 1 cut(s) 384
BstMAI GTCTC 4 cut(s) 53, 270, 303, 813
BstMBI GATC 1 cut(s) 381
BstMCI CGRYCG 1 cut(s) 511
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 283
BstPAI GACNNNNGTC 1 cut(s) 394
BstUI CGCG 2 cut(s) 17, 225
BstV1I GCAGC 3 cut(s) 129, 626, 784
BsuRI GGCC 1 cut(s) 196
BtgZI GCGATG 1 cut(s) 135
BtsCI GGATG 3 cut(s) 8, 140, 319
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 2 cut(s) 399, 710
Cac8I GCNNGC 2 cut(s) 19, 539
Cfr13I GGNCC 2 cut(s) 194, 389
CseI GACGC 2 cut(s) 23, 77
Csp6I GTAC 2 cut(s) 687, 696
CviAII CATG 5 cut(s) 190, 280, 429, 770, 846
CviJI RGCY 8 cut(s) 5, 21, 47, 132, 196, 236, 617, 750
CviKI_1 RGCY 8 cut(s) 5, 21, 47, 132, 196, 236, 617, 750
CviQI GTAC 2 cut(s) 687, 696
DdeI CTNAG 4 cut(s) 24, 213, 324, 453
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
DraIII CACNNNGTG 1 cut(s) 611
Eam1104I CTCTTC 2 cut(s) 79, 700
EarI CTCTTC 2 cut(s) 79, 700
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 780
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 813
Eco47I GGWCC 1 cut(s) 389
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 737
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 780
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 780
FaeI CATG 5 cut(s) 193, 283, 432, 773, 849
FaqI GGGAC 2 cut(s) 155, 268
FatI CATG 5 cut(s) 189, 279, 428, 769, 845
FbaI TGATCA 1 cut(s) 381
Fnu4HI GCNGC 5 cut(s) 6, 118, 237, 615, 798
FokI GGATG 2 cut(s) 127, 326
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 5 cut(s) 6, 118, 237, 615, 798
FspBI CTAG 1 cut(s) 534
GluI GCNGC 5 cut(s) 6, 118, 237, 615, 798
HaeIII GGCC 1 cut(s) 196
HgaI GACGC 2 cut(s) 23, 77
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 5 cut(s) 193, 283, 432, 773, 849
HinfI GANTC 3 cut(s) 61, 209, 803
HphI GGTGA 4 cut(s) 229, 269, 340, 560
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 5 cut(s) 13, 112, 145, 184, 386
Hpy188III TCNNGA 4 cut(s) 65, 80, 362, 506
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 2 cut(s) 17, 71
HpyAV CCTTC 2 cut(s) 398, 483
HpyCH4III ACNGT 3 cut(s) 96, 512, 626
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 5 cut(s) 8, 117, 339, 441, 614
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpyF3I CTNAG 4 cut(s) 24, 213, 324, 453
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 5 cut(s) 193, 283, 432, 773, 849
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 1 cut(s) 381
LguI GCTCTTC 1 cut(s) 700
LmnI GCTCC 1 cut(s) 457
Lsp1109I GCAGC 3 cut(s) 129, 626, 784
LweI GCATC 2 cut(s) 17, 149
MaeI CTAG 1 cut(s) 534
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 3 cut(s) 503, 512, 626
MalI GATC 1 cut(s) 383
MboI GATC 1 cut(s) 381
MboII GAAGA 4 cut(s) 66, 272, 557, 717
MhlI GDGCHC 3 cut(s) 23, 462, 712
MluCI AATT 3 cut(s) 523, 662, 813
MlyI GAGTC 2 cut(s) 55, 812
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 8 cut(s) 59, 82, 199, 304, 310, 380, 474, 664
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 2 cut(s) 306, 666
Mva1269I GAATGC 2 cut(s) 119, 779
MvnI CGCG 2 cut(s) 17, 225
MwoI GCNNNNNNNGC 1 cut(s) 14
NdeII GATC 1 cut(s) 381
NlaIII CATG 5 cut(s) 193, 283, 432, 773, 849
NlaIV GGNNCC 2 cut(s) 157, 195
NmeAIII GCCGAG 1 cut(s) 826
NmuCI GTSAC 3 cut(s) 503, 512, 626
NspI RCATGY 1 cut(s) 283
PciI ACATGT 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 700
PctI GAATGC 2 cut(s) 119, 779
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 5 cut(s) 7, 119, 238, 616, 799
PleI GAGTC 2 cut(s) 55, 811
PpsI GAGTC 2 cut(s) 55, 811
Ppu21I YACGTR 1 cut(s) 487
PscI ACATGT 1 cut(s) 279
PshAI GACNNNNGTC 1 cut(s) 394
PshBI ATTAAT 1 cut(s) 306
PsiI TTATAA 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 23
PspN4I GGNNCC 2 cut(s) 157, 195
PspPI GGNCC 2 cut(s) 194, 389
PsrI GAACNNNNNNTAC 2 cut(s) 715, 747
RsaI GTAC 2 cut(s) 688, 697
RsaNI GTAC 2 cut(s) 687, 696
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 700
SaqAI TTAA 2 cut(s) 306, 666
SatI GCNGC 5 cut(s) 6, 118, 237, 615, 798
Sau3AI GATC 1 cut(s) 381
Sau96I GGNCC 2 cut(s) 194, 389
SchI GAGTC 2 cut(s) 55, 812
SduI GDGCHC 3 cut(s) 23, 462, 712
SfaNI GCATC 2 cut(s) 17, 149
SinI GGWCC 1 cut(s) 389
Sse9I AATT 3 cut(s) 523, 662, 813
SsiI CCGC 2 cut(s) 223, 237
SspI AATATT 2 cut(s) 499, 642
SspMI CTAG 1 cut(s) 534
SstI GAGCTC 1 cut(s) 23
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 3 cut(s) 96, 512, 626
TaiI ACGT 1 cut(s) 489
TaqI TCGA 1 cut(s) 66
TasI AATT 3 cut(s) 523, 662, 813
TatI WGTACW 1 cut(s) 695
TauI GCSGC 1 cut(s) 239
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 2 cut(s) 306, 666
Tru9I TTAA 2 cut(s) 306, 666
TscAI CASTG 2 cut(s) 399, 717
TseFI GTSAC 3 cut(s) 503, 512, 626
TseI GCWGC 4 cut(s) 5, 117, 614, 797
Tsp45I GTSAC 3 cut(s) 503, 512, 626
TspDTI ATGAA 5 cut(s) 178, 347, 651, 675, 786
TspRI CASTG 2 cut(s) 399, 717
VpaK11BI GGWCC 1 cut(s) 389
VspI ATTAAT 1 cut(s) 306
XapI RAATTY 1 cut(s) 662
XceI RCATGY 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.