Rw5G035990

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001195
Physical Location & Seq
Reverse (-)
62846890 .. 62848268
1379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035990.1

Sequence Viewer

Length: 747 bp
ATGGCTGGACCCGACGCAAGCTGTGTGGATGAGATCTGCCAGGGAGATGAAACTGTTTCAAAAGGCGTAAGAAACCTAGCCATCTTCTTTGAGACTCTCGGCGCCTCTTCTCCCGATACAAGGAAACAGTTCAGTTCCAGGGACCGGGCACCTAGTTCTAAAACCAGCAATCGAAAAGGTTGCGGAGGATCCACTGGTTCAGGCACCTATACGTATACTCCTGTCGACTTCAGTTCCAAGTTCAGAGGACCAGTGGCTGGTGGAGGCCCTTCTATGTACAAAGCAGTGCCTTGCAATGAGTGTTCTAAGGAGCACCCTCCTTATTCTTATCGCCCTTACGCGCCTTGTCATATTTGTTATAAGGTTCACCACCATACTTATTGCCCTTACTTAGACCATGTCCCACAGGGTGCAACTTTTCACCATGGCTATGAAATAATTTGTGGATGTGGTAATGAATTTAATGAGGATAAGTGGGTTTGTACCTCTTGTGGTGGGAACAGAGCAGTGCTTAAGGCCAAGCATTGTTCCATTTGTAATAGCGTGAAACAGCACGGCACATATGAATGCCCGAAGGACAAGATTCTTGCTGCCAAGTATAAATTGGCCAGAGACAAGAGGCGTTCAGTTGTGCCGAATAGGATTCCCTATGAAACTGTTTGTATTCCAAAATCATCATCAGAAAAGATCTATGTTCCAACTCCTCCTCCTGGGTGGGTTTGTGGACAGATGAAAGAAGGCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

248

Amino Acids

27.16

Weight (kDa)

8.83

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AccB1I GGYRCC 3 cut(s) 101, 148, 203
AccB7I CCANNNNNTGG 1 cut(s) 257
AccI GTMKAC 2 cut(s) 215, 225
AccII CGCG 1 cut(s) 341
AciI CCGC 1 cut(s) 183
AclWI GGATC 2 cut(s) 183, 196
AcoI YGGCCR 1 cut(s) 606
AcsI RAATTY 1 cut(s) 458
AcuI CTGAAG 1 cut(s) 214
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 2 cut(s) 278, 484
AfiI CCNNNNNNNGG 4 cut(s) 120, 144, 257, 710
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 1 cut(s) 60
AjnI CCWGG 3 cut(s) 39, 137, 709
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 86, 606
AlwI GGATC 2 cut(s) 183, 196
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 3 cut(s) 265, 516, 606
ApeKI GCWGC 1 cut(s) 590
ApoI RAATTY 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 2 cut(s) 104, 343
AspS9I GGNCC 4 cut(s) 8, 142, 248, 266
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 2 cut(s) 359, 413
AvaII GGWCC 3 cut(s) 8, 142, 248
BaeGI GKGCMC 1 cut(s) 151
BalI TGGCCA 1 cut(s) 608
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 3 cut(s) 101, 148, 203
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 577
BccI CCATC 1 cut(s) 89
BceAI ACGGC 1 cut(s) 571
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 3 cut(s) 41, 139, 711
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 2 cut(s) 86, 606
BfaI CTAG 2 cut(s) 77, 153
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 512
BglII AGATCT 2 cut(s) 33, 687
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
Bme1390I CCNGG 4 cut(s) 41, 139, 146, 711
Bme18I GGWCC 3 cut(s) 8, 142, 248
BmgT120I GGNCC 4 cut(s) 8, 142, 248, 266
BmiI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 205
BmrFI CCNGG 4 cut(s) 41, 139, 146, 711
BpuMI CCSGG 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 40, 138, 424, 710
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 691, 721
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 144, 257, 710
Bse1I ACTGG 2 cut(s) 199, 251
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 3 cut(s) 41, 139, 711
BseDI CCNNGG 4 cut(s) 40, 138, 424, 710
BseGI GGATG 2 cut(s) 34, 452
BseLI CCNNNNNNNGG 4 cut(s) 120, 144, 257, 710
BseMI GCAATG 1 cut(s) 301
BseNI ACTGG 2 cut(s) 199, 251
BseRI GAGGAG 2 cut(s) 693, 696
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 1 cut(s) 577
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 3 cut(s) 267, 518, 608
BshNI GGYRCC 3 cut(s) 101, 148, 203
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 2 cut(s) 155, 386
BslI CCNNNNNNNGG 4 cut(s) 120, 144, 257, 710
BsmAI GTCTC 2 cut(s) 86, 606
BsmFI GGGAC 2 cut(s) 155, 386
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 3 cut(s) 267, 518, 608
Bsp1286I GDGCHC 2 cut(s) 151, 315
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 3 cut(s) 33, 188, 687
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 1 cut(s) 183
BspANI GGCC 3 cut(s) 267, 518, 608
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 205
BspPI GGATC 2 cut(s) 183, 196
BspT107I GGYRCC 3 cut(s) 101, 148, 203
BspTI CTTAAG 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 276
BsrI ACTGG 2 cut(s) 199, 251
BssECI CCNNGG 4 cut(s) 40, 138, 424, 710
BssMI GATC 3 cut(s) 33, 188, 687
BssNAI GTATAC 1 cut(s) 216
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 424
Bst1107I GTATAC 1 cut(s) 216
Bst2UI CCWGG 3 cut(s) 41, 139, 711
Bst4CI ACNGT 3 cut(s) 55, 129, 658
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 512
BstAUI TGTACA 1 cut(s) 276
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 306, 391
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 2 cut(s) 34, 452
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 2 cut(s) 104, 343
BstKTI GATC 3 cut(s) 36, 191, 690
BstMAI GTCTC 2 cut(s) 86, 606
BstMBI GATC 3 cut(s) 33, 188, 687
BstNI CCWGG 3 cut(s) 41, 139, 711
BstSCI CCNGG 4 cut(s) 39, 137, 144, 709
BstSLI GKGCMC 1 cut(s) 151
BstSNI TACGTA 1 cut(s) 213
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 577
BstX2I RGATCY 3 cut(s) 33, 188, 687
BstYI RGATCY 3 cut(s) 33, 188, 687
BstZ17I GTATAC 1 cut(s) 216
BsuRI GGCC 3 cut(s) 267, 518, 608
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 2 cut(s) 34, 452
BtsI GCAGTG 2 cut(s) 291, 513
BtsIMutI CAGTG 4 cut(s) 192, 258, 291, 513
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 2 cut(s) 104, 343
Cfr13I GGNCC 4 cut(s) 8, 142, 248, 266
CseI GACGC 1 cut(s) 23
Csp6I GTAC 2 cut(s) 277, 483
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 2 cut(s) 398, 425
CviJI RGCY 9 cut(s) 5, 21, 80, 257, 267, 429, 518, 608, 741
CviKI_1 RGCY 9 cut(s) 5, 21, 80, 257, 267, 429, 518, 608, 741
CviQI GTAC 2 cut(s) 277, 483
DdeI CTNAG 2 cut(s) 306, 391
DinI GGCGCC 1 cut(s) 103
DpnI GATC 3 cut(s) 35, 190, 689
DpnII GATC 3 cut(s) 33, 188, 687
DraIII CACNNNGTG 1 cut(s) 410
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Eco105I TACGTA 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 424
Eco47I GGWCC 3 cut(s) 8, 142, 248
Eco57I CTGAAG 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 3 cut(s) 39, 137, 709
EcoT14I CCWWGG 1 cut(s) 424
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 2 cut(s) 401, 428
FaqI GGGAC 2 cut(s) 155, 386
FatI CATG 2 cut(s) 397, 424
FauNDI CATATG 1 cut(s) 562
FblI GTMKAC 2 cut(s) 215, 225
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 2 cut(s) 41, 459
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 2 cut(s) 77, 153
GlaI GCGC 2 cut(s) 103, 342
GluI GCNGC 1 cut(s) 591
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 3 cut(s) 267, 518, 608
HapII CCGG 1 cut(s) 145
HgaI GACGC 1 cut(s) 23
HhaI GCGC 2 cut(s) 104, 343
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 2 cut(s) 401, 428
Hin6I GCGC 2 cut(s) 102, 341
HinP1I GCGC 2 cut(s) 102, 341
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 3 cut(s) 94, 583, 643
HpaII CCGG 1 cut(s) 145
HphI GGTGA 2 cut(s) 359, 413
Hpy166II GTNNAC 4 cut(s) 216, 226, 367, 725
Hpy188I TCNGA 2 cut(s) 245, 682
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 4 cut(s) 216, 226, 367, 725
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 279, 568, 731
HpyCH4III ACNGT 3 cut(s) 55, 129, 658
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 2 cut(s) 294, 413
HpyF3I CTNAG 2 cut(s) 306, 391
HpySE526I ACGT 1 cut(s) 212
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 2 cut(s) 401, 428
HspAI GCGC 2 cut(s) 102, 341
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 3 cut(s) 33, 188, 687
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 1 cut(s) 577
MaeI CTAG 2 cut(s) 77, 153
MaeII ACGT 1 cut(s) 212
MalI GATC 3 cut(s) 35, 190, 689
MboI GATC 3 cut(s) 33, 188, 687
MboII GAAGA 2 cut(s) 76, 99
MflI RGATCY 3 cut(s) 33, 188, 687
MhlI GDGCHC 2 cut(s) 151, 315
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 3 cut(s) 438, 458, 602
MluNI TGGCCA 1 cut(s) 608
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 722
Mox20I TGGCCA 1 cut(s) 608
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 608
MseI TTAA 2 cut(s) 462, 513
MslI CAYNNNNRTG 2 cut(s) 429, 565
Msp20I TGGCCA 1 cut(s) 608
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 4 cut(s) 41, 139, 146, 711
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 3 cut(s) 41, 139, 711
MvnI CGCG 1 cut(s) 341
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 1 cut(s) 146
NcoI CCATGG 1 cut(s) 424
NdeI CATATG 1 cut(s) 562
NdeII GATC 3 cut(s) 33, 188, 687
NlaIII CATG 2 cut(s) 401, 428
NlaIV GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 205
NmeAIII GCCGAG 1 cut(s) 78
PctI GAATGC 1 cut(s) 572
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 583, 643
PflFI GACNNNGTC 1 cut(s) 398
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 213
PsiI TTATAA 1 cut(s) 360
Psp6I CCWGG 3 cut(s) 39, 137, 709
PspGI CCWGG 3 cut(s) 39, 137, 709
PspN4I GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 205
PspPI GGNCC 4 cut(s) 8, 142, 248, 266
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 3 cut(s) 33, 188, 687
PsyI GACNNNGTC 1 cut(s) 398
RsaI GTAC 2 cut(s) 278, 484
RsaNI GTAC 2 cut(s) 277, 483
RseI CAYNNNNRTG 2 cut(s) 429, 565
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 462, 513
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 3 cut(s) 33, 188, 687
Sau96I GGNCC 4 cut(s) 8, 142, 248, 266
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 4 cut(s) 41, 139, 146, 711
SduI GDGCHC 2 cut(s) 151, 315
SetI ASST 8 cut(s) 23, 78, 154, 181, 209, 215, 366, 488
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 3 cut(s) 8, 142, 248
SmiMI CAYNNNNRTG 2 cut(s) 429, 565
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SnaBI TACGTA 1 cut(s) 213
Sse9I AATT 3 cut(s) 438, 458, 602
SsiI CCGC 1 cut(s) 183
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 77, 153
StyD4I CCNGG 4 cut(s) 39, 137, 144, 709
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 55, 129, 658
TaiI ACGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 172, 225
TasI AATT 3 cut(s) 438, 458, 602
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 2 cut(s) 583, 643
Tru1I TTAA 2 cut(s) 462, 513
Tru9I TTAA 2 cut(s) 462, 513
TscAI CASTG 4 cut(s) 199, 258, 291, 513
TseI GCWGC 1 cut(s) 590
TspDTI ATGAA 6 cut(s) 63, 447, 471, 579, 666, 746
TspRI CASTG 4 cut(s) 199, 258, 291, 513
Tth111I GACNNNGTC 1 cut(s) 398
Van91I CCANNNNNTGG 1 cut(s) 257
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 3 cut(s) 8, 142, 248
XapI RAATTY 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmiI GTMKAC 2 cut(s) 215, 225
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 2 cut(s) 77, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.