FvH4_4g36220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001195
Physical Location & Seq
Reverse (-)
33251298 .. 33252944
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36220.t1

Sequence Viewer

Length: 795 bp
ATGGCTACCGCAACCGAAACGAGCTCCCAGCAAGATGTGAAAAAACTGACCTTCTCCTTTGAGCATCTCGGCGCCTCTTCTCCCGGTTCCTATACCCCCGTCCCATTAAGGTCGGAGGTGGTGCCTTACAATGAGGATTCTAAGGTGGACCGCAAACAAACTGCCGCCTATGTTCCAATGGGGGGTGAAGATGATTTTTACATAGACATGTCCCAGTTCGCATTGTCTCGCATTAATGAGGGTTCTAAGGTGAACTGCAAACGAACAGCCACCGATATACCTGATGTCTTGGGGTCTGCGATCAGACGATCAGTCTTGGGGCCTGTGATCAGAGGACCAGTGTCGGGCGAAGGTGCTTATAAACACAAAAGACCGTGTGTGCCTTGTAATGAGTGTTCTAAGGAGCACCCCTCTTTTCCTTCTTGCCCTTATGTGCTTTGTGATCTTTGTCATGACCGTCACTACCCTATTTATTGCCCTAGCGTGCCTTGTCAAGTTTGTTATCAGGATCTTCACCTTACTCCTTATTGCCCTTACTTTCACCTTATCCCACAGGGTGCAGCTTTTGACCGTGACAACTATGAAGTAGTTTGTTGCTGTGGTAATGAATTTAATGAGGAAAAGTGGGTTTGTACCTATTGTACTGGGAGAAATGCACTGCTGAAGCGTAAGTATTGTTCCATTTGTGATAGTAGCTGGAAACAGCACAGCACCCATGAATGCCCCAAGGACAAGGTCCTTGCTACCGAGTCTAAGTCGATTAGGGACCCATTATCTTTGTCAGGGCGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

265

Amino Acids

29.55

Weight (kDa)

6.73

Isoelectric Point (pI)

58.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AccB1I GGYRCC 2 cut(s) 71, 121
AciI CCGC 3 cut(s) 9, 151, 165
AclWI GGATC 1 cut(s) 516
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 1 cut(s) 683
AcyI GRCGYC 1 cut(s) 72
AdeI CACNNNGTG 1 cut(s) 557
AfaI GTAC 2 cut(s) 634, 643
AfiI CCNNNNNNNGG 2 cut(s) 182, 344
AflIII ACRYGT 1 cut(s) 207
AhdI GACNNNNNGTC 1 cut(s) 311
AluBI AGCT 3 cut(s) 24, 563, 696
AluI AGCT 3 cut(s) 24, 563, 696
Alw21I GWGCWC 2 cut(s) 26, 408
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 1 cut(s) 516
AoxI GGCC 1 cut(s) 320
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 1 cut(s) 608
AseI ATTAAT 1 cut(s) 234
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 5 cut(s) 148, 320, 335, 736, 766
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 197, 262, 506, 533
AvaII GGWCC 4 cut(s) 148, 335, 736, 766
BanI GGYRCC 2 cut(s) 71, 121
BanII GRGCYC 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 26, 408
BbvI GCAGC 1 cut(s) 572
BclI TGATCA 1 cut(s) 327
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 2 cut(s) 165, 561
BlsI GCNGC 2 cut(s) 166, 562
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 4 cut(s) 148, 335, 736, 766
BmeRI GACNNNNNGTC 1 cut(s) 311
BmgT120I GGNCC 5 cut(s) 148, 320, 335, 736, 766
BmiI GGNNCC 6 cut(s) 73, 88, 123, 321, 767, 768
BmrFI CCNGG 1 cut(s) 84
BmrI ACTGGG 2 cut(s) 208, 654
BmsI GCATC 1 cut(s) 73
BmuI ACTGGG 2 cut(s) 208, 654
BoxI GACNNNNGTC 1 cut(s) 340
BplI GAGNNNNNCTC 2 cut(s) 395, 427
BpuMI CCSGG 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 726
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 344
Bse1I ACTGG 3 cut(s) 214, 338, 649
BseDI CCNNGG 1 cut(s) 726
BseLI CCNNNNNNNGG 2 cut(s) 182, 344
BseNI ACTGG 3 cut(s) 214, 338, 649
BseXI GCAGC 1 cut(s) 572
BseYI CCCAGC 1 cut(s) 27
BsgI GTGCAG 1 cut(s) 579
BshFI GGCC 1 cut(s) 322
BshNI GGYRCC 2 cut(s) 71, 121
BsiHKAI GWGCWC 2 cut(s) 26, 408
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 3 cut(s) 86, 196, 779
BslI CCNNNNNNNGG 2 cut(s) 182, 344
BsmAI GTCTC 1 cut(s) 231
BsmFI GGGAC 3 cut(s) 86, 196, 779
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 26, 408
Bsp143I GATC 5 cut(s) 300, 308, 327, 442, 508
BspACI CCGC 3 cut(s) 9, 151, 165
BspANI GGCC 1 cut(s) 322
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 6 cut(s) 73, 88, 123, 321, 767, 768
BspPI GGATC 1 cut(s) 516
BspT107I GGYRCC 2 cut(s) 71, 121
BsrI ACTGG 3 cut(s) 214, 338, 649
BssECI CCNNGG 1 cut(s) 726
BssMI GATC 5 cut(s) 300, 308, 327, 442, 508
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 726
Bst4CI ACNGT 3 cut(s) 375, 458, 572
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 485
BstDEI CTNAG 4 cut(s) 141, 246, 399, 753
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 5 cut(s) 303, 311, 330, 445, 511
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 5 cut(s) 300, 308, 327, 442, 508
BstNSI RCATGY 1 cut(s) 211
BstPAI GACNNNNGTC 1 cut(s) 340
BstSCI CCNGG 1 cut(s) 82
BstV1I GCAGC 1 cut(s) 572
BstX2I RGATCY 1 cut(s) 508
BstYI RGATCY 1 cut(s) 508
BsuRI GGCC 1 cut(s) 322
BtsI GCAGTG 1 cut(s) 656
BtsIMutI CAGTG 2 cut(s) 345, 656
Cac8I GCNNGC 1 cut(s) 485
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 5 cut(s) 148, 320, 335, 736, 766
Csp6I GTAC 2 cut(s) 633, 642
CviAII CATG 4 cut(s) 208, 452, 716, 792
CviJI RGCY 6 cut(s) 5, 24, 269, 322, 563, 696
CviKI_1 RGCY 6 cut(s) 5, 24, 269, 322, 563, 696
CviQI GTAC 2 cut(s) 633, 642
DdeI CTNAG 4 cut(s) 141, 246, 399, 753
DinI GGCGCC 1 cut(s) 73
DpnI GATC 5 cut(s) 302, 310, 329, 444, 510
DpnII GATC 5 cut(s) 300, 308, 327, 442, 508
DraIII CACNNNGTG 1 cut(s) 557
DriI GACNNNNNGTC 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 82
Eam1105I GACNNNNNGTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 26
Eco47I GGWCC 4 cut(s) 148, 335, 736, 766
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 683
EcoICRI GAGCTC 1 cut(s) 24
EcoO109I RGGNCCY 3 cut(s) 320, 736, 766
EcoT14I CCWWGG 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 26
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 4 cut(s) 211, 455, 719, 795
FaqI GGGAC 3 cut(s) 86, 196, 779
FatI CATG 4 cut(s) 207, 451, 715, 791
FbaI TGATCA 1 cut(s) 327
Fnu4HI GCNGC 2 cut(s) 165, 561
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 2 cut(s) 165, 561
FspBI CTAG 1 cut(s) 480
GlaI GCGC 1 cut(s) 73
GluI GCNGC 2 cut(s) 165, 561
GsaI CCCAGC 1 cut(s) 31
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 322
HapII CCGG 1 cut(s) 84
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 4 cut(s) 211, 455, 719, 795
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 2 cut(s) 137, 749
HpaII CCGG 1 cut(s) 84
HphI GGTGA 4 cut(s) 197, 262, 506, 533
Hpy166II GTNNAC 2 cut(s) 148, 253
Hpy188I TCNGA 3 cut(s) 115, 305, 332
Hpy188III TCNNGA 2 cut(s) 452, 506
Hpy8I GTNNAC 2 cut(s) 148, 253
HpyAV CCTTC 3 cut(s) 61, 344, 429
HpyCH4III ACNGT 3 cut(s) 375, 458, 572
HpyCH4V TGCA 3 cut(s) 258, 560, 656
HpyF3I CTNAG 4 cut(s) 141, 246, 399, 753
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 4 cut(s) 211, 455, 719, 795
HspAI GCGC 1 cut(s) 72
KasI GGCGCC 1 cut(s) 71
KflI GGGWCCC 1 cut(s) 766
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 5 cut(s) 300, 308, 327, 442, 508
LmnI GCTCC 2 cut(s) 29, 403
Lsp1109I GCAGC 1 cut(s) 572
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 480
MaeIII GTNAC 2 cut(s) 458, 572
MalI GATC 5 cut(s) 302, 310, 329, 444, 510
MboI GATC 5 cut(s) 300, 308, 327, 442, 508
MboII GAAGA 3 cut(s) 69, 200, 503
MflI RGATCY 1 cut(s) 508
MhlI GDGCHC 2 cut(s) 26, 408
MluCI AATT 1 cut(s) 608
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 1 cut(s) 758
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 7 cut(s) 85, 109, 127, 232, 326, 421, 610
MseI TTAA 3 cut(s) 107, 234, 612
MslI CAYNNNNRTG 1 cut(s) 206
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 725
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 84
NdeII GATC 5 cut(s) 300, 308, 327, 442, 508
NlaIII CATG 4 cut(s) 211, 455, 719, 795
NlaIV GGNNCC 6 cut(s) 73, 88, 123, 321, 767, 768
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 2 cut(s) 458, 572
NspI RCATGY 1 cut(s) 211
PagI TCATGA 1 cut(s) 451
PciI ACATGT 1 cut(s) 207
PctI GAATGC 1 cut(s) 725
PfeI GAWTC 1 cut(s) 137
PflFI GACNNNGTC 1 cut(s) 734
PkrI GCNGC 2 cut(s) 166, 562
PleI GAGTC 1 cut(s) 757
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 757
PpuMI RGGWCCY 2 cut(s) 736, 766
PscI ACATGT 1 cut(s) 207
PshAI GACNNNNGTC 1 cut(s) 340
PshBI ATTAAT 1 cut(s) 234
PsiI TTATAA 1 cut(s) 360
Psp124BI GAGCTC 1 cut(s) 26
Psp5II RGGWCCY 2 cut(s) 736, 766
PspFI CCCAGC 1 cut(s) 27
PspN4I GGNNCC 6 cut(s) 73, 88, 123, 321, 767, 768
PspPI GGNCC 5 cut(s) 148, 320, 335, 736, 766
PspPPI RGGWCCY 2 cut(s) 736, 766
PsrI GAACNNNNNNTAC 4 cut(s) 379, 411, 661, 693
PsuI RGATCY 1 cut(s) 508
PsyI GACNNNGTC 1 cut(s) 734
RsaI GTAC 2 cut(s) 634, 643
RsaNI GTAC 2 cut(s) 633, 642
RseI CAYNNNNRTG 1 cut(s) 206
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 3 cut(s) 107, 234, 612
SatI GCNGC 2 cut(s) 165, 561
Sau3AI GATC 5 cut(s) 300, 308, 327, 442, 508
Sau96I GGNCC 5 cut(s) 148, 320, 335, 736, 766
SchI GAGTC 1 cut(s) 758
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 2 cut(s) 26, 408
SfaNI GCATC 1 cut(s) 73
SfoI GGCGCC 1 cut(s) 73
SinI GGWCC 4 cut(s) 148, 335, 736, 766
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 1 cut(s) 608
SsiI CCGC 3 cut(s) 9, 151, 165
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 1 cut(s) 480
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 3 cut(s) 375, 458, 572
TaqI TCGA 1 cut(s) 758
TasI AATT 1 cut(s) 608
TatI WGTACW 1 cut(s) 641
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 3 cut(s) 107, 234, 612
Tru9I TTAA 3 cut(s) 107, 234, 612
TscAI CASTG 2 cut(s) 345, 663
TseFI GTSAC 2 cut(s) 458, 572
TseI GCWGC 1 cut(s) 560
Tsp45I GTSAC 2 cut(s) 458, 572
TspDTI ATGAA 3 cut(s) 597, 621, 732
TspRI CASTG 2 cut(s) 345, 663
Tth111I GACNNNGTC 1 cut(s) 734
VpaK11BI GGWCC 4 cut(s) 148, 335, 736, 766
VspI ATTAAT 1 cut(s) 234
XapI RAATTY 1 cut(s) 608
XceI RCATGY 1 cut(s) 211
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.