FvH4_5g38300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001195
Physical Location & Seq
Reverse (-)
28331281 .. 28334006
2726 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38300.t1

Sequence Viewer

Length: 639 bp
ATGGTGTCTAAGCTTAGCAGAGAAGAGATGATTAAAGTTGTTGACCAAGCCAAGCACGCCCTTGCTGCTGAGTGCCTCACAGACCAGTTAGAAGAGATGGATCTGAATAATGAAGGTTCCTATACCCCCATCCCATTCAGGTCGGAGTTCATGGTGCCTTACAATGAGGATTCGAAGGTGAATAGCAAACAAACAGCCACCTCTAGTCCAGTGGGGGGTGAACTTTCTTACATAGTCGTGTCCCAGTTCATAGTGCCTGACAATGAGGGTTCGAACTGCAAACAAACAGCCACCTATGGTCCAGTGGGGGGCATAGACATGTCCCAGTTCATAGTGCCTCACAATGAGGGTTCGAACTGCGAACAAACTGCCACCTATGGTCCAGTGGGGGCCATAGACATGTCCCAGTTCATAGTGCCTCACGTTAATCAGGGTTCTAAGGTGAACTGCGAACAAACAGCCACCTATATTCCTGTTGGCTTGAGCTCAAGTGAAGATTATTATGGAGACATGTTCCAGTTCGTAGTGCCTGACAATGAGGGTTCTAAGGTGAACAGCAAACAAACAGCCACCTATTCTCCTGTTAGTGTGATGCCCATGTTCAGAGGACCAGTGACAGGTGAAGGTTCTTACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

23.08

Weight (kDa)

4.51

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AclWI GGATC 1 cut(s) 108
AfiI CCNNNNNNNGG 3 cut(s) 215, 308, 617
AflIII ACRYGT 3 cut(s) 318, 399, 510
AluBI AGCT 2 cut(s) 13, 486
AluI AGCT 2 cut(s) 13, 486
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 1 cut(s) 501
AlwI GGATC 1 cut(s) 108
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 1 cut(s) 65
AspS9I GGNCC 4 cut(s) 299, 380, 390, 608
AsuHPI GGTGA 5 cut(s) 190, 230, 454, 562, 632
AsuII TTCGAA 3 cut(s) 173, 272, 353
AvaII GGWCC 3 cut(s) 299, 380, 608
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 488
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 1 cut(s) 52
BccI CCATC 2 cut(s) 91, 137
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BcoDI GTCTC 1 cut(s) 501
BfaI CTAG 1 cut(s) 204
BisI GCNGC 1 cut(s) 66
BlpI GCTNAGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 67
Bme18I GGWCC 3 cut(s) 299, 380, 608
BmgT120I GGNCC 4 cut(s) 299, 380, 390, 608
BmiI GGNNCC 3 cut(s) 118, 156, 391
BmrI ACTGGG 3 cut(s) 238, 319, 400
BmsI GCATC 1 cut(s) 582
BmuI ACTGGG 3 cut(s) 238, 319, 400
Bpu1102I GCTNAGC 1 cut(s) 14
Bpu14I TTCGAA 3 cut(s) 173, 272, 353
BpuEI CTTGAG 2 cut(s) 472, 502
BsaXI ACNNNNNCTCC 2 cut(s) 562, 592
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 308, 617
Bse1I ACTGG 9 cut(s) 85, 209, 244, 302, 325, 383, 406, 517, 611
BseGI GGATG 1 cut(s) 129
BseLI CCNNNNNNNGG 3 cut(s) 215, 308, 617
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 9 cut(s) 85, 209, 244, 302, 325, 383, 406, 517, 611
BseXI GCAGC 1 cut(s) 52
BshFI GGCC 1 cut(s) 392
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 488
BslFI GGGAC 3 cut(s) 226, 307, 388
BslI CCNNNNNNNGG 3 cut(s) 215, 308, 617
BsmAI GTCTC 1 cut(s) 501
BsmFI GGGAC 3 cut(s) 226, 307, 388
BsnI GGCC 1 cut(s) 392
Bsp119I TTCGAA 3 cut(s) 173, 272, 353
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 1 cut(s) 100
Bsp1720I GCTNAGC 1 cut(s) 14
BspANI GGCC 1 cut(s) 392
BspCNI CTCAG 1 cut(s) 61
BspLI GGNNCC 3 cut(s) 118, 156, 391
BspPI GGATC 1 cut(s) 108
BspT104I TTCGAA 3 cut(s) 173, 272, 353
BspT107I GGYRCC 1 cut(s) 154
BsrI ACTGG 9 cut(s) 85, 209, 244, 302, 325, 383, 406, 517, 611
BssMI GATC 1 cut(s) 100
Bst6I CTCTTC 2 cut(s) 18, 87
BstBI TTCGAA 3 cut(s) 173, 272, 353
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 5 cut(s) 9, 14, 69, 438, 546
BstF5I GGATG 1 cut(s) 129
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 2 cut(s) 56, 65
BstNSI RCATGY 3 cut(s) 322, 403, 514
BstV1I GCAGC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 1 cut(s) 392
BtsCI GGATG 1 cut(s) 129
BtsIMutI CAGTG 4 cut(s) 216, 309, 390, 618
Cac8I GCNNGC 1 cut(s) 57
Cfr13I GGNCC 4 cut(s) 299, 380, 390, 608
CviAII CATG 5 cut(s) 151, 319, 400, 511, 598
CviJI RGCY 9 cut(s) 13, 50, 197, 290, 392, 461, 480, 486, 569
CviKI_1 RGCY 9 cut(s) 13, 50, 197, 290, 392, 461, 480, 486, 569
DdeI CTNAG 5 cut(s) 9, 14, 69, 438, 546
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 18, 87
EarI CTCTTC 2 cut(s) 18, 87
Ecl136II GAGCTC 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 488
Eco47I GGWCC 3 cut(s) 299, 380, 608
Eco53kI GAGCTC 1 cut(s) 486
EcoICRI GAGCTC 1 cut(s) 486
EcoT38I GRGCYC 1 cut(s) 488
FaeI CATG 5 cut(s) 154, 322, 403, 514, 601
FaqI GGGAC 3 cut(s) 226, 307, 388
FatI CATG 5 cut(s) 150, 318, 399, 510, 597
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 1 cut(s) 204
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 1 cut(s) 392
Hin1II CATG 5 cut(s) 154, 322, 403, 514, 601
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 1 cut(s) 170
HphI GGTGA 5 cut(s) 190, 230, 454, 562, 632
Hpy166II GTNNAC 4 cut(s) 43, 221, 445, 553
Hpy188I TCNGA 3 cut(s) 105, 145, 605
Hpy8I GTNNAC 4 cut(s) 43, 221, 445, 553
HpyAV CCTTC 3 cut(s) 107, 169, 617
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 1 cut(s) 279
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 65
HpyF3I CTNAG 5 cut(s) 9, 14, 69, 438, 546
HpySE526I ACGT 1 cut(s) 423
Hsp92II CATG 5 cut(s) 154, 322, 403, 514, 601
Kzo9I GATC 1 cut(s) 100
Lsp1109I GCAGC 1 cut(s) 52
LweI GCATC 1 cut(s) 582
MaeI CTAG 1 cut(s) 204
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 1 cut(s) 613
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 3 cut(s) 35, 104, 506
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 9 cut(s) 86, 160, 211, 259, 340, 348, 429, 532, 599
MseI TTAA 2 cut(s) 33, 426
MslI CAYNNNNRTG 3 cut(s) 236, 317, 398
MwoI GCNNNNNNNGC 2 cut(s) 56, 65
NdeII GATC 1 cut(s) 100
NlaIII CATG 5 cut(s) 154, 322, 403, 514, 601
NlaIV GGNNCC 3 cut(s) 118, 156, 391
NmuCI GTSAC 1 cut(s) 613
NspI RCATGY 3 cut(s) 322, 403, 514
NspV TTCGAA 3 cut(s) 173, 272, 353
PciI ACATGT 3 cut(s) 318, 399, 510
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 1 cut(s) 67
PscI ACATGT 3 cut(s) 318, 399, 510
Psp124BI GAGCTC 1 cut(s) 488
PspN4I GGNNCC 3 cut(s) 118, 156, 391
PspPI GGNCC 4 cut(s) 299, 380, 390, 608
PsuI RGATCY 1 cut(s) 100
RseI CAYNNNNRTG 3 cut(s) 236, 317, 398
SacI GAGCTC 1 cut(s) 488
SaqAI TTAA 2 cut(s) 33, 426
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 4 cut(s) 299, 380, 390, 608
SduI GDGCHC 1 cut(s) 488
SfaNI GCATC 1 cut(s) 582
SfuI TTCGAA 3 cut(s) 173, 272, 353
SinI GGWCC 3 cut(s) 299, 380, 608
SmiMI CAYNNNNRTG 3 cut(s) 236, 317, 398
SmlI CTYRAG 2 cut(s) 481, 487
SmoI CTYRAG 2 cut(s) 481, 487
SspMI CTAG 1 cut(s) 204
SstI GAGCTC 1 cut(s) 488
TaiI ACGT 1 cut(s) 426
TaqI TCGA 3 cut(s) 173, 272, 353
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 33, 426
Tru9I TTAA 2 cut(s) 33, 426
TscAI CASTG 4 cut(s) 216, 309, 390, 618
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 5 cut(s) 126, 139, 238, 319, 400
TspRI CASTG 4 cut(s) 216, 309, 390, 618
VpaK11BI GGWCC 3 cut(s) 299, 380, 608
XceI RCATGY 3 cut(s) 322, 403, 514
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.