Rh5AG381900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001195
Physical Location & Seq
Reverse (-)
65561504 .. 65563075
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG381900.1

Sequence Viewer

Length: 753 bp
ATGGCTGGACCCGACGCAAGCTGTACGGATGAGATCTGCCAGGGAGATGAAACTGTTTCAAAAGGCGTAAGAAACCTAGCCATCTTCTTTGAGACTCTCGGCGCCTCTTCTCCCGATACAAGGAAACAGTTCAGTTCCAGGGCCCGGGCACCTAGTTCTAAAATCAGCAATCGAAATCGAAAAGGTTGCGGAGGATCCACTGGTTCAGGTTTTGCCTTAGGCACCTATACCTATACTCCTGTCGACTTCAGGTCCAAGTTCAGAAAACCAGTGACTGGTGGAGATGCTTCTATGGACAAAGCAGTGCCTTGCAATGAGTGTTCTAAGGAGCACCCCCCTTATTCTTATTGCCCTTACGCACCTTGTCATATTTGTTATAAGGTTCACCACCATAGTTATTGCCCTTACTTTGACCATATCCCACAAGGTGCAACTTTTGACTGTGGCTATCAAATAATTTGTGGATGTGGTAGTGAATTTAATGAGGATAAGTGGGTTTGTACCTCTTGTGGTGGGAGCAGAGCAATGCTTAAGGCCAAGTATTGTAACATTTGTAAAAGTGTGTTACAGCACCGCGCATATGAATGCCCGAAGGACAAGGTTCTTGCCGCCAAGTATAAATTGGCCAGGGACAAGAGGCGTTCAGTTGTGCCAAATAGGATTCCCTATGACATTGCCTATATACCAGAATCATCATCAGAAAAGATCTATGTTCCAACTCCTCCTCCTGGGTGGGTTTGTGGACACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

27.56

Weight (kDa)

8.8

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
AccB1I GGYRCC 3 cut(s) 101, 148, 221
AccB7I CCANNNNNTGG 1 cut(s) 275
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 576
AciI CCGC 3 cut(s) 189, 574, 609
AclWI GGATC 2 cut(s) 189, 202
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 428
AfaI GTAC 2 cut(s) 25, 502
AfiI CCNNNNNNNGG 4 cut(s) 120, 144, 275, 728
AflII CTTAAG 1 cut(s) 530
AgsI TTSAA 1 cut(s) 60
AhdI GACNNNNNGTC 1 cut(s) 250
AjnI CCWGG 4 cut(s) 39, 137, 626, 727
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 2 cut(s) 189, 202
AlwNI CAGNNNCTG 1 cut(s) 275
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 3 cut(s) 141, 534, 624
ApaI GGGCCC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 476
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 2 cut(s) 104, 578
AspS9I GGNCC 4 cut(s) 8, 141, 142, 252
AsuC2I CCSGG 2 cut(s) 145, 146
AsuHPI GGTGA 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 8, 252
AxyI CCTNAGG 1 cut(s) 217
BaeGI GKGCMC 2 cut(s) 145, 151
BalI TGGCCA 1 cut(s) 626
BamHI GGATCC 1 cut(s) 194
BanI GGYRCC 3 cut(s) 101, 148, 221
BanII GRGCYC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 333
BccI CCATC 1 cut(s) 89
BcgI CGANNNNNNTGC 2 cut(s) 168, 202
BciT130I CCWGG 4 cut(s) 41, 139, 628, 729
BcnI CCSGG 2 cut(s) 145, 146
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 2 cut(s) 77, 153
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 530
BglII AGATCT 2 cut(s) 33, 705
BisI GCNGC 1 cut(s) 609
BlsI GCNGC 1 cut(s) 610
Bme1390I CCNGG 6 cut(s) 41, 139, 145, 146, 628, 729
Bme18I GGWCC 2 cut(s) 8, 252
BmeRI GACNNNNNGTC 1 cut(s) 250
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 4 cut(s) 8, 141, 142, 252
BmiI GGNNCC 6 cut(s) 10, 103, 143, 150, 196, 223
BmrFI CCNGG 6 cut(s) 41, 139, 145, 146, 628, 729
BmsI GCATC 1 cut(s) 274
BpuMI CCSGG 2 cut(s) 145, 146
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 5 cut(s) 40, 138, 144, 627, 728
BsaXI ACNNNNNCTCC 4 cut(s) 220, 250, 709, 739
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 144, 275, 728
Bse1I ACTGG 3 cut(s) 205, 269, 280
Bse21I CCTNAGG 1 cut(s) 217
Bse3DI GCAATG 3 cut(s) 319, 531, 672
BseBI CCWGG 4 cut(s) 41, 139, 628, 729
BseDI CCNNGG 5 cut(s) 40, 138, 144, 627, 728
BseGI GGATG 2 cut(s) 34, 470
BseLI CCNNNNNNNGG 4 cut(s) 120, 144, 275, 728
BseMI GCAATG 3 cut(s) 319, 531, 672
BseNI ACTGG 3 cut(s) 205, 269, 280
BseRI GAGGAG 2 cut(s) 711, 714
BseSI GKGCMC 2 cut(s) 145, 151
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 3 cut(s) 143, 536, 626
BshNI GGYRCC 3 cut(s) 101, 148, 221
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 4 cut(s) 120, 144, 275, 728
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 644
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 3 cut(s) 143, 536, 626
BsoBI CYCGRG 1 cut(s) 144
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 3 cut(s) 145, 151, 333
Bsp143I GATC 3 cut(s) 33, 194, 705
BspACI CCGC 3 cut(s) 189, 574, 609
BspANI GGCC 3 cut(s) 143, 536, 626
BspFNI CGCG 1 cut(s) 576
BspLI GGNNCC 6 cut(s) 10, 103, 143, 150, 196, 223
BspPI GGATC 2 cut(s) 189, 202
BspT107I GGYRCC 3 cut(s) 101, 148, 221
BspTI CTTAAG 1 cut(s) 530
BsrDI GCAATG 3 cut(s) 319, 531, 672
BsrI ACTGG 3 cut(s) 205, 269, 280
BssECI CCNNGG 5 cut(s) 40, 138, 144, 627, 728
BssMI GATC 3 cut(s) 33, 194, 705
BssNI GRCGYC 1 cut(s) 102
Bst2UI CCWGG 4 cut(s) 41, 139, 628, 729
Bst4CI ACNGT 3 cut(s) 55, 129, 443
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 217, 324
BstF5I GGATG 2 cut(s) 34, 470
BstFNI CGCG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 2 cut(s) 104, 578
BstKTI GATC 3 cut(s) 36, 197, 708
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 3 cut(s) 33, 194, 705
BstNI CCWGG 4 cut(s) 41, 139, 628, 729
BstSCI CCNGG 6 cut(s) 39, 137, 143, 144, 626, 727
BstSLI GKGCMC 2 cut(s) 145, 151
BstUI CGCG 1 cut(s) 576
BstX2I RGATCY 3 cut(s) 33, 194, 705
BstYI RGATCY 3 cut(s) 33, 194, 705
Bsu36I CCTNAGG 1 cut(s) 217
BsuRI GGCC 3 cut(s) 143, 536, 626
BtsCI GGATG 2 cut(s) 34, 470
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 3 cut(s) 198, 276, 309
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 275
CfoI GCGC 2 cut(s) 104, 578
Cfr13I GGNCC 4 cut(s) 8, 141, 142, 252
Cfr9I CCCGGG 1 cut(s) 144
CseI GACGC 1 cut(s) 23
Csp6I GTAC 2 cut(s) 24, 501
CviAII CATG 1 cut(s) 750
CviJI RGCY 7 cut(s) 5, 21, 80, 143, 447, 536, 626
CviKI_1 RGCY 7 cut(s) 5, 21, 80, 143, 447, 536, 626
CviQI GTAC 2 cut(s) 24, 501
DdeI CTNAG 2 cut(s) 217, 324
DinI GGCGCC 1 cut(s) 103
DpnI GATC 3 cut(s) 35, 196, 707
DpnII GATC 3 cut(s) 33, 194, 705
DraIII CACNNNGTG 1 cut(s) 428
DriI GACNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 1 cut(s) 112
Eam1105I GACNNNNNGTC 1 cut(s) 250
EarI CTCTTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 8, 252
Eco57I CTGAAG 1 cut(s) 232
Eco81I CCTNAGG 1 cut(s) 217
Eco88I CYCGRG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 141
EcoRII CCWGG 4 cut(s) 39, 137, 626, 727
EcoT38I GRGCYC 1 cut(s) 145
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
FaeI CATG 1 cut(s) 753
FaqI GGGAC 1 cut(s) 644
FatI CATG 1 cut(s) 749
FauNDI CATATG 1 cut(s) 580
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 1 cut(s) 609
FokI GGATG 2 cut(s) 41, 477
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 1 cut(s) 609
FspBI CTAG 2 cut(s) 77, 153
GlaI GCGC 2 cut(s) 103, 577
GluI GCNGC 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 3 cut(s) 143, 536, 626
HapII CCGG 1 cut(s) 145
HgaI GACGC 1 cut(s) 23
HhaI GCGC 2 cut(s) 104, 578
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 1 cut(s) 753
Hin6I GCGC 2 cut(s) 102, 576
HinP1I GCGC 2 cut(s) 102, 576
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 3 cut(s) 94, 661, 689
HpaII CCGG 1 cut(s) 145
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 3 cut(s) 244, 385, 743
Hpy188I TCNGA 2 cut(s) 263, 700
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 3 cut(s) 244, 385, 743
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 586
HpyCH4III ACNGT 3 cut(s) 55, 129, 443
HpyCH4V TGCA 2 cut(s) 312, 431
HpyF3I CTNAG 2 cut(s) 217, 324
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 1 cut(s) 753
HspAI GCGC 2 cut(s) 102, 576
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 3 cut(s) 33, 194, 705
LmnI GCTCC 2 cut(s) 328, 516
LweI GCATC 1 cut(s) 274
MaeI CTAG 2 cut(s) 77, 153
MaeIII GTNAC 3 cut(s) 271, 545, 564
MalI GATC 3 cut(s) 35, 196, 707
MboI GATC 3 cut(s) 33, 194, 705
MboII GAAGA 2 cut(s) 76, 99
MflI RGATCY 3 cut(s) 33, 194, 705
MhlI GDGCHC 3 cut(s) 145, 151, 333
MlsI TGGCCA 1 cut(s) 626
MluCI AATT 3 cut(s) 456, 476, 620
MluNI TGGCCA 1 cut(s) 626
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 740
MnlI CCTC 7 cut(s) 115, 185, 478, 514, 630, 732, 735
Mox20I TGGCCA 1 cut(s) 626
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 626
MseI TTAA 2 cut(s) 480, 531
MslI CAYNNNNRTG 1 cut(s) 583
Msp20I TGGCCA 1 cut(s) 626
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 6 cut(s) 41, 139, 145, 146, 628, 729
Mva1269I GAATGC 1 cut(s) 590
MvaI CCWGG 4 cut(s) 41, 139, 628, 729
MvnI CGCG 1 cut(s) 576
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 2 cut(s) 145, 146
NdeI CATATG 1 cut(s) 580
NdeII GATC 3 cut(s) 33, 194, 705
NlaIII CATG 1 cut(s) 753
NlaIV GGNNCC 6 cut(s) 10, 103, 143, 150, 196, 223
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 271
PctI GAATGC 1 cut(s) 590
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 661, 689
PflMI CCANNNNNTGG 1 cut(s) 275
PkrI GCNGC 1 cut(s) 610
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 88
PsiI TTATAA 1 cut(s) 378
Psp6I CCWGG 4 cut(s) 39, 137, 626, 727
PspGI CCWGG 4 cut(s) 39, 137, 626, 727
PspN4I GGNNCC 6 cut(s) 10, 103, 143, 150, 196, 223
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 4 cut(s) 8, 141, 142, 252
PstNI CAGNNNCTG 1 cut(s) 275
PsuI RGATCY 3 cut(s) 33, 194, 705
RsaI GTAC 2 cut(s) 25, 502
RsaNI GTAC 2 cut(s) 24, 501
RseI CAYNNNNRTG 1 cut(s) 583
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 2 cut(s) 480, 531
SatI GCNGC 1 cut(s) 609
Sau3AI GATC 3 cut(s) 33, 194, 705
Sau96I GGNCC 4 cut(s) 8, 141, 142, 252
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 6 cut(s) 41, 139, 145, 146, 628, 729
SduI GDGCHC 3 cut(s) 145, 151, 333
SfaNI GCATC 1 cut(s) 274
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 2 cut(s) 8, 252
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 583
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SrfI GCCCGGGC 1 cut(s) 146
Sse9I AATT 3 cut(s) 456, 476, 620
SsiI CCGC 3 cut(s) 189, 574, 609
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 77, 153
StyD4I CCNGG 6 cut(s) 39, 137, 143, 144, 626, 727
TaaI ACNGT 3 cut(s) 55, 129, 443
TaqI TCGA 3 cut(s) 172, 178, 243
TasI AATT 3 cut(s) 456, 476, 620
TauI GCSGC 1 cut(s) 611
TfiI GAWTC 2 cut(s) 661, 689
Tru1I TTAA 2 cut(s) 480, 531
Tru9I TTAA 2 cut(s) 480, 531
TscAI CASTG 3 cut(s) 205, 276, 309
TseFI GTSAC 1 cut(s) 271
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 2 cut(s) 63, 597
TspGWI ACGGA 1 cut(s) 41
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 3 cut(s) 205, 276, 309
Van91I CCANNNNNTGG 1 cut(s) 275
Vha464I CTTAAG 1 cut(s) 530
VpaK11BI GGWCC 2 cut(s) 8, 252
XapI RAATTY 1 cut(s) 476
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 2 cut(s) 77, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.