Rh5DG408900

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001195
Physical Location & Seq
Reverse (-)
64748002 .. 64749528
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG408900.1

Sequence Viewer

Length: 774 bp
ATGGCTGGACCCGACGCAAGCTGTGTGAATGAGATCTGCCAGGGAGATGAAATTGTTTCAAAAGGCGTAAGAAACCTGGCCATCTTCTTTGAGACTCTCGGCGCCTCTTCTCCCGATACAAGGAAACAGTTCAGTTTCAGGGCCCGGGCACCTAGTTTTAAAACCAGCAATCGAAAAGGTTGCGGAGGATCCGCTGGTTCAGGCACCTATACCTATACTCCTGTCGACTTCAGGTCCAAGTTCAGAGGACCAGTGGCTGGTGAAGATGCTTCAATGGACGAAGCAGTGCCTTGCAATGAGTGTTCTAAGGAGCACCCCCCTTATTCTTATTGCCCTTACAGACCTGACAGGCCTTGTCGAATTTGTTATAACGTTCACCACCCTAGTCATTGCCCTTACTTTGACCTTATCCCACAGGGTGCAACTTTTGACCGTGGCTATGAAATAATTTGTGGATGTGGTAATGAATTTAATGAGGATAAGTGGGTTTGTACCTCATGTGGTGGGAGCAGAGCAGTGCTTAAGCCCAAGTATTGTTCCATTTGTAATAGTGTGAAACAGCACGGCACATATGAATGCCCGAAGGACAAGGTTCTTGCTGCTAAGTATAAATTGGCCAGAGACCAGATACGTTCAGTTGTGCCGAATTTGATTCCTTATGACACTGTCTATATTCCAAAATCATCATCAGGAAAGATCTATGTTCCAACTCCTCCTCCTGGGTGGGTTTGTGGACACATGAAAGAAGGCATGCTTACTGGGTCTACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

28.23

Weight (kDa)

8.6

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
AccB1I GGYRCC 3 cut(s) 101, 148, 203
AccB7I CCANNNNNTGG 1 cut(s) 257
AccI GTMKAC 2 cut(s) 225, 764
AciI CCGC 2 cut(s) 183, 192
AclI AACGTT 1 cut(s) 372
AclWI GGATC 2 cut(s) 183, 196
AcoI YGGCCR 2 cut(s) 78, 615
AcsI RAATTY 3 cut(s) 360, 467, 646
AcuI CTGAAG 1 cut(s) 214
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 419
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 3 cut(s) 120, 257, 719
AflII CTTAAG 1 cut(s) 521
AgsI TTSAA 2 cut(s) 60, 273
AhdI GACNNNNNGTC 1 cut(s) 232
AjnI CCWGG 3 cut(s) 39, 75, 718
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 86, 615
AlwI GGATC 2 cut(s) 183, 196
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 4 cut(s) 78, 141, 350, 615
ApaI GGGCCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 599
ApoI RAATTY 3 cut(s) 360, 467, 646
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 5 cut(s) 8, 141, 142, 234, 248
AsuC2I CCSGG 2 cut(s) 145, 146
AsuHPI GGTGA 2 cut(s) 272, 368
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 3 cut(s) 8, 234, 248
BaeGI GKGCMC 2 cut(s) 145, 151
BalI TGGCCA 2 cut(s) 80, 617
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 3 cut(s) 101, 148, 203
BanII GRGCYC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 586
BccI CCATC 1 cut(s) 89
BceAI ACGGC 1 cut(s) 580
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 3 cut(s) 41, 77, 720
BcnI CCSGG 2 cut(s) 145, 146
BcoDI GTCTC 2 cut(s) 86, 615
BfaI CTAG 2 cut(s) 153, 384
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 521
BglII AGATCT 2 cut(s) 33, 696
BisI GCNGC 1 cut(s) 600
BlsI GCNGC 1 cut(s) 601
Bme1390I CCNGG 5 cut(s) 41, 77, 145, 146, 720
Bme18I GGWCC 3 cut(s) 8, 234, 248
BmeRI GACNNNNNGTC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 5 cut(s) 8, 141, 142, 234, 248
BmiI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 205
BmrFI CCNGG 5 cut(s) 41, 77, 145, 146, 720
BmrI ACTGGG 1 cut(s) 768
BmsI GCATC 1 cut(s) 256
BmuI ACTGGG 1 cut(s) 768
BpuMI CCSGG 2 cut(s) 145, 146
BsaHI GRCGYC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 615
BsaJI CCNNGG 4 cut(s) 40, 144, 433, 719
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 700, 730
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 257, 719
Bse1I ACTGG 2 cut(s) 251, 763
Bse3DI GCAATG 2 cut(s) 301, 388
BseBI CCWGG 3 cut(s) 41, 77, 720
BseDI CCNNGG 4 cut(s) 40, 144, 433, 719
BseGI GGATG 1 cut(s) 461
BseLI CCNNNNNNNGG 3 cut(s) 120, 257, 719
BseMI GCAATG 2 cut(s) 301, 388
BseNI ACTGG 2 cut(s) 251, 763
BseRI GAGGAG 2 cut(s) 702, 705
BseSI GKGCMC 2 cut(s) 145, 151
BseXI GCAGC 1 cut(s) 586
BshFI GGCC 4 cut(s) 80, 143, 352, 617
BshNI GGYRCC 3 cut(s) 101, 148, 203
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 1 cut(s) 145
BslI CCNNNNNNNGG 3 cut(s) 120, 257, 719
BsmAI GTCTC 2 cut(s) 86, 615
BsmI GAATGC 1 cut(s) 581
BsnI GGCC 4 cut(s) 80, 143, 352, 617
Bso31I GGTCTC 1 cut(s) 615
BsoBI CYCGRG 1 cut(s) 144
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 3 cut(s) 145, 151, 315
Bsp143I GATC 3 cut(s) 33, 188, 696
BspACI CCGC 2 cut(s) 183, 192
BspANI GGCC 4 cut(s) 80, 143, 352, 617
BspLI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 205
BspPI GGATC 2 cut(s) 183, 196
BspT107I GGYRCC 3 cut(s) 101, 148, 203
BspTI CTTAAG 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 615
BsrDI GCAATG 2 cut(s) 301, 388
BsrI ACTGG 2 cut(s) 251, 763
BssECI CCNNGG 4 cut(s) 40, 144, 433, 719
BssMI GATC 3 cut(s) 33, 188, 696
BssNI GRCGYC 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 41, 77, 720
Bst4CI ACNGT 3 cut(s) 129, 434, 667
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 19, 752
BstDEI CTNAG 2 cut(s) 306, 603
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 1 cut(s) 461
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 3 cut(s) 36, 191, 699
BstMAI GTCTC 2 cut(s) 86, 615
BstMBI GATC 3 cut(s) 33, 188, 696
BstNI CCWGG 3 cut(s) 41, 77, 720
BstNSI RCATGY 1 cut(s) 754
BstSCI CCNGG 5 cut(s) 39, 75, 143, 144, 718
BstSLI GKGCMC 2 cut(s) 145, 151
BstV1I GCAGC 1 cut(s) 586
BstX2I RGATCY 3 cut(s) 33, 188, 696
BstYI RGATCY 3 cut(s) 33, 188, 696
BsuRI GGCC 4 cut(s) 80, 143, 352, 617
BtgI CCRYGG 1 cut(s) 433
BtsCI GGATG 1 cut(s) 461
BtsI GCAGTG 2 cut(s) 291, 522
BtsIMutI CAGTG 4 cut(s) 258, 291, 522, 663
Cac8I GCNNGC 2 cut(s) 19, 752
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 5 cut(s) 8, 141, 142, 234, 248
Cfr9I CCCGGG 1 cut(s) 144
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 492
CviAII CATG 3 cut(s) 498, 739, 751
CviJI RGCY 9 cut(s) 5, 21, 80, 143, 257, 352, 438, 526, 617
CviKI_1 RGCY 9 cut(s) 5, 21, 80, 143, 257, 352, 438, 526, 617
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 2 cut(s) 306, 603
DinI GGCGCC 1 cut(s) 103
DpnI GATC 3 cut(s) 35, 190, 698
DpnII GATC 3 cut(s) 33, 188, 696
DraI TTTAAA 1 cut(s) 160
DraIII CACNNNGTG 1 cut(s) 419
DriI GACNNNNNGTC 1 cut(s) 232
EaeI YGGCCR 2 cut(s) 78, 615
Eam1104I CTCTTC 1 cut(s) 112
Eam1105I GACNNNNNGTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 112
Eco147I AGGCCT 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 615
Eco47I GGWCC 3 cut(s) 8, 234, 248
Eco57I CTGAAG 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 141
EcoRII CCWGG 3 cut(s) 39, 75, 718
EcoT38I GRGCYC 1 cut(s) 145
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
FaeI CATG 3 cut(s) 501, 742, 754
FalI AAGNNNNNCTT 2 cut(s) 738, 770
FatI CATG 3 cut(s) 497, 738, 750
FauNDI CATATG 1 cut(s) 571
FblI GTMKAC 2 cut(s) 225, 764
Fnu4HI GCNGC 1 cut(s) 600
FokI GGATG 1 cut(s) 468
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 1 cut(s) 600
FspBI CTAG 2 cut(s) 153, 384
GlaI GCGC 1 cut(s) 103
GluI GCNGC 1 cut(s) 600
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 4 cut(s) 80, 143, 352, 617
HapII CCGG 1 cut(s) 145
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 501, 742, 754
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 2 cut(s) 94, 652
HpaII CCGG 1 cut(s) 145
HphI GGTGA 2 cut(s) 272, 368
Hpy166II GTNNAC 4 cut(s) 226, 376, 734, 765
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 2 cut(s) 113, 690
Hpy8I GTNNAC 4 cut(s) 226, 376, 734, 765
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 2 cut(s) 577, 740
HpyCH4III ACNGT 3 cut(s) 129, 434, 667
HpyCH4IV ACGT 2 cut(s) 372, 631
HpyCH4V TGCA 2 cut(s) 294, 422
HpyF3I CTNAG 2 cut(s) 306, 603
HpySE526I ACGT 2 cut(s) 372, 631
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 501, 742, 754
HspAI GCGC 1 cut(s) 102
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 3 cut(s) 33, 188, 696
LmnI GCTCC 2 cut(s) 310, 507
Lsp1109I GCAGC 1 cut(s) 586
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 153, 384
MaeII ACGT 2 cut(s) 372, 631
MalI GATC 3 cut(s) 35, 190, 698
MboI GATC 3 cut(s) 33, 188, 696
MboII GAAGA 3 cut(s) 76, 99, 275
MflI RGATCY 3 cut(s) 33, 188, 696
MhlI GDGCHC 3 cut(s) 145, 151, 315
MlsI TGGCCA 2 cut(s) 80, 617
MluCI AATT 6 cut(s) 51, 360, 447, 467, 611, 646
MluNI TGGCCA 2 cut(s) 80, 617
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 731
MnlI CCTC 7 cut(s) 115, 179, 239, 469, 505, 723, 726
Mox20I TGGCCA 2 cut(s) 80, 617
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 2 cut(s) 80, 617
MseI TTAA 3 cut(s) 159, 471, 522
MslI CAYNNNNRTG 1 cut(s) 574
Msp20I TGGCCA 2 cut(s) 80, 617
MspA1I CMGCKG 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 5 cut(s) 41, 77, 145, 146, 720
Mva1269I GAATGC 1 cut(s) 581
MvaI CCWGG 3 cut(s) 41, 77, 720
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 2 cut(s) 145, 146
NdeI CATATG 1 cut(s) 571
NdeII GATC 3 cut(s) 33, 188, 696
NlaIII CATG 3 cut(s) 501, 742, 754
NlaIV GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 205
NmeAIII GCCGAG 1 cut(s) 78
NspI RCATGY 1 cut(s) 754
PaeI GCATGC 1 cut(s) 754
PceI AGGCCT 1 cut(s) 352
PctI GAATGC 1 cut(s) 581
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 652
PflFI GACNNNGTC 1 cut(s) 665
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 1 cut(s) 601
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 88
PsiI TTATAA 1 cut(s) 369
Psp1406I AACGTT 1 cut(s) 372
Psp6I CCWGG 3 cut(s) 39, 75, 718
PspGI CCWGG 3 cut(s) 39, 75, 718
PspN4I GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 205
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 5 cut(s) 8, 141, 142, 234, 248
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 3 cut(s) 33, 188, 696
PsyI GACNNNGTC 1 cut(s) 665
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 1 cut(s) 574
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 3 cut(s) 159, 471, 522
SatI GCNGC 1 cut(s) 600
Sau3AI GATC 3 cut(s) 33, 188, 696
Sau96I GGNCC 5 cut(s) 8, 141, 142, 234, 248
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 5 cut(s) 41, 77, 145, 146, 720
SduI GDGCHC 3 cut(s) 145, 151, 315
SfaNI GCATC 1 cut(s) 256
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 3 cut(s) 8, 234, 248
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 574
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SphI GCATGC 1 cut(s) 754
SrfI GCCCGGGC 1 cut(s) 146
Sse9I AATT 6 cut(s) 51, 360, 447, 467, 611, 646
SseBI AGGCCT 1 cut(s) 352
SsiI CCGC 2 cut(s) 183, 192
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 153, 384
StuI AGGCCT 1 cut(s) 352
StyD4I CCNGG 5 cut(s) 39, 75, 143, 144, 718
TaaI ACNGT 3 cut(s) 129, 434, 667
TaiI ACGT 2 cut(s) 375, 634
TaqI TCGA 3 cut(s) 172, 225, 358
TasI AATT 6 cut(s) 51, 360, 447, 467, 611, 646
TfiI GAWTC 1 cut(s) 652
Tru1I TTAA 3 cut(s) 159, 471, 522
Tru9I TTAA 3 cut(s) 159, 471, 522
TscAI CASTG 4 cut(s) 258, 291, 522, 670
TseI GCWGC 1 cut(s) 599
TspDTI ATGAA 5 cut(s) 63, 456, 480, 588, 755
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 4 cut(s) 258, 291, 522, 670
Tth111I GACNNNGTC 1 cut(s) 665
Van91I CCANNNNNTGG 1 cut(s) 257
Vha464I CTTAAG 1 cut(s) 521
VpaK11BI GGWCC 3 cut(s) 8, 234, 248
XapI RAATTY 3 cut(s) 360, 467, 646
XceI RCATGY 1 cut(s) 754
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 2 cut(s) 225, 764
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 2 cut(s) 153, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.