FvH4_4g36171

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001195
Physical Location & Seq
Reverse (-)
33225336 .. 33226483
1148 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36171.t1

Sequence Viewer

Length: 612 bp
ATGGCTTCTGCAACCGAAACGAGCTCCCAGCAAGATGTGAAAAAACTGACCTTCTCCTTTGAGAATCTCGGCACCTCTTCTCCCGGTACAAAGAAACCGTTGAGTTCCAGCAGCGATGGCTCCTATCCTCCCATCCCGTTCAGGTCGGAGTTCATAGTGCCGGATCCTGAGGTGAACTACAAACAAACAGCCGCCTATATTCGAGTGGGGGGTCCGTGGCGGGTGAAGGTGCTTATAGTGAAGAAAGTCGACCAAGTGTGCCTTGCGATGAGTGTTCTAAGGAGCACCCCACTTATTCTCGTTGCCCTTACTTGCCTTGTGACCTTTGTCATCAGCGTCACCACCCTTCTTATTGCCCTGCCGTGCCTTGTAAAATTTGTTATCAGCATCTTCACCCCACTTCTAGTTGCCCTTACCCCTCCTCCATTTCCCACAGGGAGTATACCTGTGCTTAAGAAGAAGTATTGTTCCATTTGTAATAGTAGCTACGTACAGCACAGCACACATGAATGCCCAAAGGACGAGGTTCTTGCTGCTGAGTATAAATCGGTTAGAGACCATCGTCTTTCAGTCGGGGCCAAAGTGATTCCCTATGACGAGGGTTCTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.1

Weight (kDa)

9.0

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 2 cut(s) 249, 442
AciI CCGC 2 cut(s) 192, 220
AclWI GGATC 2 cut(s) 158, 171
AcsI RAATTY 1 cut(s) 374
AfaI GTAC 2 cut(s) 88, 492
AflII CTTAAG 1 cut(s) 452
AluBI AGCT 2 cut(s) 24, 486
AluI AGCT 2 cut(s) 24, 486
Alw21I GWGCWC 2 cut(s) 26, 287
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 2 cut(s) 158, 171
AoxI GGCC 1 cut(s) 576
ApeKI GCWGC 2 cut(s) 111, 533
ApoI RAATTY 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 212, 576
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 184, 235, 331, 385
AvaII GGWCC 1 cut(s) 212
AxyI CCTNAGG 1 cut(s) 168
BamHI GGATCC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 26, 287
BbvI GCAGC 2 cut(s) 123, 520
BccI CCATC 3 cut(s) 110, 140, 567
BceAI ACGGC 1 cut(s) 346
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 1 cut(s) 404
BfrI CTTAAG 1 cut(s) 452
BisI GCNGC 3 cut(s) 112, 192, 534
BlsI GCNGC 3 cut(s) 113, 193, 535
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 212, 576
BmiI GGNNCC 5 cut(s) 73, 121, 165, 213, 577
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 396
BoxI GACNNNNGTC 2 cut(s) 326, 561
BpuMI CCSGG 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 490
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 215
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 406, 436
Bse21I CCTNAGG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 215
BseGI GGATG 1 cut(s) 132
BseMII CTCAG 2 cut(s) 159, 528
BseRI GAGGAG 1 cut(s) 411
BseXI GCAGC 2 cut(s) 123, 520
BseYI CCCAGC 1 cut(s) 27
BshFI GGCC 1 cut(s) 578
BshNI GGYRCC 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 26, 287
BsiSI CCGG 2 cut(s) 84, 161
BsmAI GTCTC 1 cut(s) 549
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 1 cut(s) 578
Bso31I GGTCTC 1 cut(s) 549
Bsp1286I GDGCHC 2 cut(s) 26, 287
Bsp143I GATC 1 cut(s) 163
BspACI CCGC 2 cut(s) 192, 220
BspANI GGCC 1 cut(s) 578
BspCNI CTCAG 2 cut(s) 160, 529
BspLI GGNNCC 5 cut(s) 73, 121, 165, 213, 577
BspPI GGATC 2 cut(s) 158, 171
BspT107I GGYRCC 1 cut(s) 71
BspTI CTTAAG 1 cut(s) 452
BspTNI GGTCTC 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 1 cut(s) 163
BssNAI GTATAC 1 cut(s) 443
Bst1107I GTATAC 1 cut(s) 443
Bst4CI ACNGT 1 cut(s) 99
Bst6I CTCTTC 1 cut(s) 82
BstAFI CTTAAG 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 490
BstDEI CTNAG 3 cut(s) 168, 278, 537
BstDSI CCRYGG 1 cut(s) 215
BstF5I GGATG 1 cut(s) 132
BstKTI GATC 1 cut(s) 166
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstPAI GACNNNNGTC 2 cut(s) 326, 561
BstSCI CCNGG 1 cut(s) 82
BstSNI TACGTA 1 cut(s) 490
BstV1I GCAGC 2 cut(s) 123, 520
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BstZ17I GTATAC 1 cut(s) 443
Bsu36I CCTNAGG 1 cut(s) 168
BsuRI GGCC 1 cut(s) 578
BtgI CCRYGG 1 cut(s) 215
BtgZI GCGATG 2 cut(s) 129, 281
BtsCI GGATG 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 212, 576
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 87, 491
CviAII CATG 1 cut(s) 506
CviJI RGCY 6 cut(s) 5, 24, 120, 191, 486, 578
CviKI_1 RGCY 6 cut(s) 5, 24, 120, 191, 486, 578
CviQI GTAC 2 cut(s) 87, 491
DdeI CTNAG 3 cut(s) 168, 278, 537
DpnI GATC 1 cut(s) 165
DpnII GATC 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 24
Eco105I TACGTA 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 26
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 24
Eco81I CCTNAGG 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 26
FaeI CATG 1 cut(s) 509
FaiI YATR 7 cut(s) 155, 198, 236, 443, 507, 543, 594
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FatI CATG 1 cut(s) 505
FauI CCCGC 1 cut(s) 213
FblI GTMKAC 2 cut(s) 249, 442
Fnu4HI GCNGC 3 cut(s) 112, 192, 534
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 3 cut(s) 112, 192, 534
FspBI CTAG 1 cut(s) 404
GluI GCNGC 3 cut(s) 112, 192, 534
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 1 cut(s) 578
HapII CCGG 2 cut(s) 84, 161
HgaI GACGC 1 cut(s) 325
Hin1II CATG 1 cut(s) 509
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 2 cut(s) 64, 586
HpaII CCGG 2 cut(s) 84, 161
HphI GGTGA 4 cut(s) 184, 235, 331, 385
Hpy166II GTNNAC 3 cut(s) 175, 250, 443
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 3 cut(s) 175, 250, 443
HpyAV CCTTC 3 cut(s) 61, 220, 356
HpyCH4III ACNGT 1 cut(s) 99
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 1 cut(s) 11
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 3 cut(s) 168, 278, 537
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 1 cut(s) 509
Kzo9I GATC 1 cut(s) 163
LmnI GCTCC 3 cut(s) 29, 125, 282
LpnPI CCDG 9 cut(s) 41, 97, 121, 127, 174, 180, 371, 420, 459
Lsp1109I GCAGC 2 cut(s) 123, 520
LweI GCATC 1 cut(s) 396
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 2 cut(s) 319, 337
MalI GATC 1 cut(s) 165
MboI GATC 1 cut(s) 163
MboII GAAGA 4 cut(s) 69, 253, 382, 469
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 26, 287
MluCI AATT 1 cut(s) 374
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 7 cut(s) 85, 138, 163, 429, 432, 517, 592
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 508
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 2 cut(s) 84, 161
MspR9I CCNGG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 515
MwoI GCNNNNNNNGC 1 cut(s) 117
NciI CCSGG 1 cut(s) 84
NdeII GATC 1 cut(s) 163
NlaIII CATG 1 cut(s) 509
NlaIV GGNNCC 5 cut(s) 73, 121, 165, 213, 577
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 2 cut(s) 319, 337
PctI GAATGC 1 cut(s) 515
PfeI GAWTC 2 cut(s) 64, 586
PkrI GCNGC 3 cut(s) 113, 193, 535
Ppu21I YACGTR 1 cut(s) 490
PshAI GACNNNNGTC 2 cut(s) 326, 561
Psp124BI GAGCTC 1 cut(s) 26
PspFI CCCAGC 1 cut(s) 27
PspN4I GGNNCC 5 cut(s) 73, 121, 165, 213, 577
PspPI GGNCC 2 cut(s) 212, 576
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 2 cut(s) 88, 492
RsaNI GTAC 2 cut(s) 87, 491
RseI CAYNNNNRTG 1 cut(s) 508
SacI GAGCTC 1 cut(s) 26
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 3 cut(s) 112, 192, 534
Sau3AI GATC 1 cut(s) 163
Sau96I GGNCC 2 cut(s) 212, 576
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 2 cut(s) 26, 287
SfaNI GCATC 1 cut(s) 396
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 508
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SnaBI TACGTA 1 cut(s) 490
Sse9I AATT 1 cut(s) 374
SsiI CCGC 2 cut(s) 192, 220
SspMI CTAG 1 cut(s) 404
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 99
TaiI ACGT 1 cut(s) 492
TaqI TCGA 2 cut(s) 202, 249
TasI AATT 1 cut(s) 374
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 2 cut(s) 64, 586
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TseFI GTSAC 2 cut(s) 319, 337
TseI GCWGC 2 cut(s) 111, 533
Tsp45I GTSAC 2 cut(s) 319, 337
TspDTI ATGAA 2 cut(s) 142, 522
TspGWI ACGGA 1 cut(s) 204
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 374
XmiI GTMKAC 2 cut(s) 249, 442
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.