FvH4_4g24132

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001195
Physical Location & Seq
Forward (+)
26415492 .. 26416535
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24132.t1

Sequence Viewer

Length: 933 bp
ATGGCTGCACCCAGAGATGTAAGAAAGCTTAACCACTTGTTTGAGACTCTCGGCACCTCTTCTCCTAATACAATAAGGAAACCGTTCAGTTCCTGCGGCGTCTATCTATCAGAAAGAGCAGGCCTTTTATGGCTGGCTGGTCGAGATGCTTCTATGAATAAAGGACCTAACAATGAGGATTATTCTAAGGTGAACTCCAAACAAACAGTCATGCATACTCCTGCTGGCTATAGTTCCAGGTCCGGAGAACCAGTGGCACCTGACTATAGAGAAGAAAGACCATGTATGCCTTGCGATTTGGATTATTTTAAGTTCCAGGTCAGGAGAGAAAAACTAGTGGTGCCTGACATTAGAGAAGAATTACCAAGTGTGCCTTGCAATGAGTGTTCTAAGGAGCACCCCGCTTATTCTTATTGCCCTTACGCGCCTTGTAGGGTTTGTTACAAGGTTCACCACCCTACTAATTGTCCTTACTTCCACTATATCCCACAGGGTGCAGCTTTTGACCGTGTTGGCTATGAGTTAGTTTGTAGTTGTGGTAACTTATTTAACGAGGATAAGTGGGTTTGTTCATTTTGTCGTCGAAGCATGCCAAAGCTCAAGGCGAAGTATTGTACCATCTGTAATAGCTTTGATGATCACAGCACACATGACTGCCCGAAGGATAAGGTTCTTGCTGCCGAGTATAAACAGATTAGAGACTATATGCTTTCAGCTGGGTCTGATCTGTCTCCCTATGACAAAGCAAGGTTAGAGGTCATCATTTGGTCCGCGGGCCTAAAAGTCAGTGGGCGGCCCGTTGGTCTGAAGGGTCAAAGCCGCCACCCGGCCCGGCCCATAGAAAAGCCCATCTCTGCCCGGCCTATAGGGCATGAGGCCGGGCTAGGGCGGAGGGTTCAAAGCCTCGGCAAGGTTACTGGGTCTACCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

34.82

Weight (kDa)

8.97

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 53, 256, 340
AccI GTMKAC 1 cut(s) 923
AccII CGCG 2 cut(s) 425, 773
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 7 cut(s) 96, 402, 771, 773, 793, 820, 889
AcuI CTGAAG 1 cut(s) 827
AcyI GRCGYC 1 cut(s) 99
AdeI CACNNNGTG 1 cut(s) 494
AfaI GTAC 1 cut(s) 616
AfiI CCNNNNNNNGG 3 cut(s) 826, 885, 910
AgsI TTSAA 1 cut(s) 899
AhlI ACTAGT 1 cut(s) 334
AjnI CCWGG 2 cut(s) 236, 315
AluBI AGCT 5 cut(s) 28, 500, 598, 630, 716
AluI AGCT 5 cut(s) 28, 500, 598, 630, 716
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 3 cut(s) 38, 693, 735
AlwNI CAGNNNCTG 1 cut(s) 93
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 7 cut(s) 121, 775, 794, 828, 833, 860, 876
ApeKI GCWGC 3 cut(s) 5, 497, 677
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 7 cut(s) 164, 240, 768, 775, 795, 829, 834
AsuC2I CCSGG 4 cut(s) 827, 832, 859, 880
AsuHPI GGTGA 2 cut(s) 202, 443
AvaII GGWCC 3 cut(s) 164, 240, 768
BanI GGYRCC 3 cut(s) 53, 256, 340
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 2 cut(s) 509, 664
BccI CCATC 2 cut(s) 626, 857
BciT130I CCWGG 2 cut(s) 238, 317
BclI TGATCA 1 cut(s) 637
BcnI CCSGG 4 cut(s) 827, 832, 859, 880
BcoDI GTCTC 3 cut(s) 38, 693, 735
BcuI ACTAGT 1 cut(s) 334
BfaI CTAG 2 cut(s) 335, 884
BfmI CTRYAG 3 cut(s) 229, 265, 864
BglI GCCNNNNNGGC 1 cut(s) 868
BisI GCNGC 6 cut(s) 6, 97, 498, 678, 794, 820
BlsI GCNGC 6 cut(s) 7, 98, 499, 679, 795, 821
Bme1390I CCNGG 6 cut(s) 238, 317, 827, 832, 859, 880
Bme18I GGWCC 3 cut(s) 164, 240, 768
BmgT120I GGNCC 7 cut(s) 164, 240, 768, 775, 795, 829, 834
BmiI GGNNCC 3 cut(s) 55, 258, 342
BmrFI CCNGG 6 cut(s) 238, 317, 827, 832, 859, 880
BmrI ACTGGG 1 cut(s) 927
BmsI GCATC 1 cut(s) 136
BmuI ACTGGG 1 cut(s) 927
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 4 cut(s) 827, 832, 859, 880
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 771, 904
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 237, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 826, 885, 910
Bse1I ACTGG 2 cut(s) 251, 922
Bse3DI GCAATG 1 cut(s) 385
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 2 cut(s) 238, 317
BseDI CCNNGG 2 cut(s) 771, 904
BseLI CCNNNNNNNGG 3 cut(s) 826, 885, 910
BseMI GCAATG 1 cut(s) 385
BseNI ACTGG 2 cut(s) 251, 922
BseXI GCAGC 2 cut(s) 509, 664
BseYI CCCAGC 1 cut(s) 716
BsgI GTGCAG 1 cut(s) 516
Bsh1236I CGCG 2 cut(s) 425, 773
BshFI GGCC 7 cut(s) 123, 777, 796, 830, 835, 862, 878
BshNI GGYRCC 3 cut(s) 53, 256, 340
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 5 cut(s) 243, 827, 832, 859, 879
BslI CCNNNNNNNGG 3 cut(s) 826, 885, 910
BsmAI GTCTC 3 cut(s) 38, 693, 735
BsnI GGCC 7 cut(s) 123, 777, 796, 830, 835, 862, 878
Bsp1286I GDGCHC 1 cut(s) 399
Bsp13I TCCGGA 1 cut(s) 242
Bsp143I GATC 2 cut(s) 637, 724
BspACI CCGC 7 cut(s) 96, 402, 771, 773, 793, 820, 889
BspANI GGCC 7 cut(s) 123, 777, 796, 830, 835, 862, 878
BspEI TCCGGA 1 cut(s) 242
BspFNI CGCG 2 cut(s) 425, 773
BspLI GGNNCC 3 cut(s) 55, 258, 342
BspT107I GGYRCC 3 cut(s) 53, 256, 340
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 2 cut(s) 251, 922
BssECI CCNNGG 2 cut(s) 771, 904
BssMI GATC 2 cut(s) 637, 724
BssNI GRCGYC 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 238, 317
Bst4CI ACNGT 3 cut(s) 84, 208, 509
Bst6I CTCTTC 1 cut(s) 64
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 5 cut(s) 121, 135, 226, 590, 775
BstDEI CTNAG 2 cut(s) 186, 390
BstDSI CCRYGG 1 cut(s) 771
BstENI CCTNNNNNAGG 1 cut(s) 908
BstFNI CGCG 2 cut(s) 425, 773
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 2 cut(s) 640, 727
BstMAI GTCTC 3 cut(s) 38, 693, 735
BstMBI GATC 2 cut(s) 637, 724
BstMWI GCNNNNNNNGC 1 cut(s) 868
BstNI CCWGG 2 cut(s) 238, 317
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 6 cut(s) 236, 315, 825, 830, 857, 878
BstSFI CTRYAG 3 cut(s) 229, 265, 864
BstUI CGCG 2 cut(s) 425, 773
BstV1I GCAGC 2 cut(s) 509, 664
BsuRI GGCC 7 cut(s) 123, 777, 796, 830, 835, 862, 878
BtgI CCRYGG 1 cut(s) 771
BtsIMutI CAGTG 2 cut(s) 258, 793
Cac8I GCNNGC 5 cut(s) 121, 135, 226, 590, 775
CaiI CAGNNNCTG 1 cut(s) 93
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 7 cut(s) 164, 240, 768, 775, 795, 829, 834
Cfr42I CCGCGG 1 cut(s) 774
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 615
CviAII CATG 5 cut(s) 211, 282, 589, 650, 872
CviQI GTAC 1 cut(s) 615
DdeI CTNAG 2 cut(s) 186, 390
DpnI GATC 2 cut(s) 639, 726
DpnII GATC 2 cut(s) 637, 724
DraIII CACNNNGTG 1 cut(s) 494
Eam1104I CTCTTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 64
EciI GGCGGA 1 cut(s) 904
Eco147I AGGCCT 1 cut(s) 123
Eco47I GGWCC 3 cut(s) 164, 240, 768
Eco57I CTGAAG 1 cut(s) 827
EcoNI CCTNNNNNAGG 1 cut(s) 908
EcoO109I RGGNCCY 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 236, 315
EcoT22I ATGCAT 1 cut(s) 216
FaeI CATG 5 cut(s) 214, 285, 592, 653, 875
FalI AAGNNNNNCTT 2 cut(s) 358, 390
FatI CATG 5 cut(s) 210, 281, 588, 649, 871
FauI CCCGC 2 cut(s) 409, 766
FbaI TGATCA 1 cut(s) 637
FblI GTMKAC 1 cut(s) 923
Fnu4HI GCNGC 6 cut(s) 6, 97, 498, 678, 794, 820
Fsp4HI GCNGC 6 cut(s) 6, 97, 498, 678, 794, 820
FspBI CTAG 2 cut(s) 335, 884
GlaI GCGC 1 cut(s) 426
GluI GCNGC 6 cut(s) 6, 97, 498, 678, 794, 820
GsaI CCCAGC 1 cut(s) 720
HaeIII GGCC 7 cut(s) 123, 777, 796, 830, 835, 862, 878
HapII CCGG 5 cut(s) 243, 827, 832, 859, 879
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 427
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 5 cut(s) 214, 285, 592, 653, 875
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 46
HpaII CCGG 5 cut(s) 243, 827, 832, 859, 879
HphI GGTGA 2 cut(s) 202, 443
Hpy166II GTNNAC 3 cut(s) 193, 451, 924
Hpy188I TCNGA 3 cut(s) 112, 724, 807
Hpy188III TCNNGA 3 cut(s) 143, 243, 322
Hpy8I GTNNAC 3 cut(s) 193, 451, 924
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 2 cut(s) 655, 802
HpyCH4III ACNGT 3 cut(s) 84, 208, 509
HpyCH4V TGCA 4 cut(s) 8, 214, 378, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 868
HpyF3I CTNAG 2 cut(s) 186, 390
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 5 cut(s) 214, 285, 592, 653, 875
HspAI GCGC 1 cut(s) 425
Kpn2I TCCGGA 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 637
KspI CCGCGG 1 cut(s) 774
Kzo9I GATC 2 cut(s) 637, 724
LmnI GCTCC 1 cut(s) 394
Lsp1109I GCAGC 2 cut(s) 509, 664
LweI GCATC 1 cut(s) 136
MaeI CTAG 2 cut(s) 335, 884
MaeIII GTNAC 3 cut(s) 440, 539, 913
MalI GATC 2 cut(s) 639, 726
MboI GATC 2 cut(s) 637, 724
MboII GAAGA 3 cut(s) 51, 284, 368
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 2 cut(s) 359, 463
MlyI GAGTC 1 cut(s) 40
MnlI CCTC 7 cut(s) 67, 169, 547, 748, 868, 885, 914
Mph1103I ATGCAT 1 cut(s) 216
MroI TCCGGA 1 cut(s) 242
MroXI GAANNNNTTC 1 cut(s) 83
MseI TTAA 3 cut(s) 30, 309, 549
MspA1I CMGCKG 2 cut(s) 716, 773
MspI CCGG 5 cut(s) 243, 827, 832, 859, 879
MspR9I CCNGG 6 cut(s) 238, 317, 827, 832, 859, 880
MvaI CCWGG 2 cut(s) 238, 317
MvnI CGCG 2 cut(s) 425, 773
MwoI GCNNNNNNNGC 1 cut(s) 868
NciI CCSGG 4 cut(s) 827, 832, 859, 880
NdeII GATC 2 cut(s) 637, 724
NlaIII CATG 5 cut(s) 214, 285, 592, 653, 875
NlaIV GGNNCC 3 cut(s) 55, 258, 342
NmeAIII GCCGAG 3 cut(s) 30, 706, 885
NsiI ATGCAT 1 cut(s) 216
NspI RCATGY 1 cut(s) 592
PaeI GCATGC 1 cut(s) 592
PceI AGGCCT 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 83
PkrI GCNGC 6 cut(s) 7, 98, 499, 679, 795, 821
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
PpuMI RGGWCCY 1 cut(s) 164
Psp5II RGGWCCY 1 cut(s) 164
Psp6I CCWGG 2 cut(s) 236, 315
PspFI CCCAGC 1 cut(s) 716
PspGI CCWGG 2 cut(s) 236, 315
PspN4I GGNNCC 3 cut(s) 55, 258, 342
PspPI GGNCC 7 cut(s) 164, 240, 768, 775, 795, 829, 834
PspPPI RGGWCCY 1 cut(s) 164
PstNI CAGNNNCTG 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 1 cut(s) 616
RsaNI GTAC 1 cut(s) 615
SacII CCGCGG 1 cut(s) 774
SaqAI TTAA 3 cut(s) 30, 309, 549
SatI GCNGC 6 cut(s) 6, 97, 498, 678, 794, 820
Sau3AI GATC 2 cut(s) 637, 724
Sau96I GGNCC 7 cut(s) 164, 240, 768, 775, 795, 829, 834
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 6 cut(s) 238, 317, 827, 832, 859, 880
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 1 cut(s) 136
SfcI CTRYAG 3 cut(s) 229, 265, 864
Sfr303I CCGCGG 1 cut(s) 774
SgrBI CCGCGG 1 cut(s) 774
SinI GGWCC 3 cut(s) 164, 240, 768
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
SpeI ACTAGT 1 cut(s) 334
SphI GCATGC 1 cut(s) 592
Sse9I AATT 2 cut(s) 359, 463
SseBI AGGCCT 1 cut(s) 123
SsiI CCGC 7 cut(s) 96, 402, 771, 773, 793, 820, 889
SspMI CTAG 2 cut(s) 335, 884
StuI AGGCCT 1 cut(s) 123
StyD4I CCNGG 6 cut(s) 236, 315, 825, 830, 857, 878
TaaI ACNGT 3 cut(s) 84, 208, 509
TaqI TCGA 2 cut(s) 142, 583
TasI AATT 2 cut(s) 359, 463
TauI GCSGC 3 cut(s) 99, 796, 822
Tru1I TTAA 3 cut(s) 30, 309, 549
Tru9I TTAA 3 cut(s) 30, 309, 549
TscAI CASTG 2 cut(s) 258, 793
TseI GCWGC 3 cut(s) 5, 497, 677
TspDTI ATGAA 2 cut(s) 170, 561
TspRI CASTG 2 cut(s) 258, 793
VpaK11BI GGWCC 3 cut(s) 164, 240, 768
XagI CCTNNNNNAGG 1 cut(s) 908
XceI RCATGY 1 cut(s) 592
XmiI GTMKAC 1 cut(s) 923
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 2 cut(s) 335, 884
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.