Rh5BG393900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001195
Physical Location & Seq
Reverse (-)
63358418 .. 63381827
23410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG393900.1

Sequence Viewer

Length: 759 bp
ATGGCTGGACCCGACGCAAGCTGTGTAGATGAGATCTGCCAGGGAGATGAAACTATTTCAAAAGGCGTAAGAAACCTAGCCATCTTCTTTGAGACTCTCGGCGCCTCTTCTCCGGATACAAGGAAACAGTTCAGTTCTAGGGCCCGGGCACCTAGTTCTAAAACCAGCAATCGAAAAGGTTGCGGAGGATCCACTGGTTCAGGTTTTGCCTTAGGCACCTATACCTATACTCCTGTCGACTTCAGTTCCAAGTTCAGAGGACCAGTGGCTGGTGGAGGCCCTTCTATGGACAAAGCAGTGCCCTGCAATGAGTGTTCTAAGGAGCACCCTCCTTATTCTTATTGCCCTTACGCGCCTTGTCATATTTGTTATAAGGTTCACCACCATAGTTATTGCCCTTACTTCGACCATGTCCCACAGGGTGCAACTTTTCACCATGGCTATGAAATAATTTGTGGATGTGGTAATGAATTTAATGAGGATAAGTGGGTTTGTACCTCTTGTGGTGGGAGCAGAGCAGTGCTTAAGGCCAAGCATTGTTCCATATGTAATAGCGTGAAACAGCACGGCACATATGAATGCCCGAAGGACAAGGTTCTGGCTGCCAAGTATAAATTGGCCAGAGACAAGAGGCGTTCAGTTGTGCCGAATAGGATTCCCTATGAAACTGTTTGTATTCCAAAATCATCATCAGAAAAGATCTATGTTCCAACTCCTCCTCCTGGGTGGGTTTGTGGACACATGAAAGAAGGCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

27.41

Weight (kDa)

8.73

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccB1I GGYRCC 3 cut(s) 101, 148, 215
AccB7I CCANNNNNTGG 1 cut(s) 269
AccI GTMKAC 1 cut(s) 237
AccII CGCG 1 cut(s) 353
AccIII TCCGGA 1 cut(s) 112
AciI CCGC 1 cut(s) 183
AclWI GGATC 2 cut(s) 183, 196
AcoI YGGCCR 1 cut(s) 618
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 422
AfaI GTAC 1 cut(s) 496
AfiI CCNNNNNNNGG 3 cut(s) 269, 286, 722
AflII CTTAAG 1 cut(s) 524
AgsI TTSAA 1 cut(s) 60
AjnI CCWGG 2 cut(s) 39, 721
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 2 cut(s) 86, 618
AlwI GGATC 2 cut(s) 183, 196
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 144
Aor13HI TCCGGA 1 cut(s) 112
AoxI GGCC 4 cut(s) 141, 277, 528, 618
ApaI GGGCCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 602
ApoI RAATTY 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 2 cut(s) 104, 355
AspS9I GGNCC 5 cut(s) 8, 141, 142, 260, 278
AsuC2I CCSGG 2 cut(s) 145, 146
AsuHPI GGTGA 2 cut(s) 371, 425
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 8, 260
AxyI CCTNAGG 1 cut(s) 211
BaeGI GKGCMC 3 cut(s) 145, 151, 303
BalI TGGCCA 1 cut(s) 620
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 3 cut(s) 101, 148, 215
BanII GRGCYC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 589
BccI CCATC 1 cut(s) 89
BceAI ACGGC 1 cut(s) 583
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 2 cut(s) 41, 723
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 2 cut(s) 145, 146
BcoDI GTCTC 2 cut(s) 86, 618
BfaI CTAG 3 cut(s) 77, 138, 153
BfoI RGCGCY 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 524
BfuI GTATCC 1 cut(s) 109
BglII AGATCT 2 cut(s) 33, 699
BisI GCNGC 1 cut(s) 603
BlsI GCNGC 1 cut(s) 604
Bme1390I CCNGG 4 cut(s) 41, 145, 146, 723
Bme18I GGWCC 2 cut(s) 8, 260
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 5 cut(s) 8, 141, 142, 260, 278
BmiI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 217
BmrFI CCNGG 4 cut(s) 41, 145, 146, 723
BpuMI CCSGG 2 cut(s) 145, 146
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 40, 144, 436, 722
BsaWI WCCGGW 1 cut(s) 112
BsaXI ACNNNNNCTCC 4 cut(s) 214, 244, 703, 733
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 286, 722
Bse1I ACTGG 2 cut(s) 199, 263
Bse21I CCTNAGG 1 cut(s) 211
Bse3DI GCAATG 1 cut(s) 313
BseAI TCCGGA 1 cut(s) 112
BseBI CCWGG 2 cut(s) 41, 723
BseDI CCNNGG 4 cut(s) 40, 144, 436, 722
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 3 cut(s) 269, 286, 722
BseMI GCAATG 1 cut(s) 313
BseNI ACTGG 2 cut(s) 199, 263
BseRI GAGGAG 2 cut(s) 705, 708
BseSI GKGCMC 3 cut(s) 145, 151, 303
BseXI GCAGC 1 cut(s) 589
Bsh1236I CGCG 1 cut(s) 353
BshFI GGCC 4 cut(s) 143, 279, 530, 620
BshNI GGYRCC 3 cut(s) 101, 148, 215
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 2 cut(s) 113, 145
BslFI GGGAC 1 cut(s) 398
BslI CCNNNNNNNGG 3 cut(s) 269, 286, 722
BsmAI GTCTC 2 cut(s) 86, 618
BsmFI GGGAC 1 cut(s) 398
BsmI GAATGC 1 cut(s) 584
BsnI GGCC 4 cut(s) 143, 279, 530, 620
BsoBI CYCGRG 1 cut(s) 144
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 4 cut(s) 145, 151, 303, 327
Bsp13I TCCGGA 1 cut(s) 112
Bsp143I GATC 3 cut(s) 33, 188, 699
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 1 cut(s) 183
BspANI GGCC 4 cut(s) 143, 279, 530, 620
BspEI TCCGGA 1 cut(s) 112
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 217
BspPI GGATC 2 cut(s) 183, 196
BspT107I GGYRCC 3 cut(s) 101, 148, 215
BspTI CTTAAG 1 cut(s) 524
BsrDI GCAATG 1 cut(s) 313
BsrI ACTGG 2 cut(s) 199, 263
BssECI CCNNGG 4 cut(s) 40, 144, 436, 722
BssMI GATC 3 cut(s) 33, 188, 699
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 2 cut(s) 41, 723
Bst4CI ACNGT 2 cut(s) 129, 670
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 211, 318
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 1 cut(s) 464
BstFNI CGCG 1 cut(s) 353
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 2 cut(s) 104, 355
BstKTI GATC 3 cut(s) 36, 191, 702
BstMAI GTCTC 2 cut(s) 86, 618
BstMBI GATC 3 cut(s) 33, 188, 699
BstNI CCWGG 2 cut(s) 41, 723
BstSCI CCNGG 4 cut(s) 39, 143, 144, 721
BstSLI GKGCMC 3 cut(s) 145, 151, 303
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 1 cut(s) 589
BstX2I RGATCY 3 cut(s) 33, 188, 699
BstYI RGATCY 3 cut(s) 33, 188, 699
Bsu36I CCTNAGG 1 cut(s) 211
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 4 cut(s) 143, 279, 530, 620
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 1 cut(s) 464
BtsI GCAGTG 2 cut(s) 303, 525
BtsIMutI CAGTG 4 cut(s) 192, 270, 303, 525
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 269
CfoI GCGC 2 cut(s) 104, 355
Cfr13I GGNCC 5 cut(s) 8, 141, 142, 260, 278
Cfr9I CCCGGG 1 cut(s) 144
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 495
CviAII CATG 3 cut(s) 410, 437, 742
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 2 cut(s) 211, 318
DinI GGCGCC 1 cut(s) 103
DpnI GATC 3 cut(s) 35, 190, 701
DpnII GATC 3 cut(s) 33, 188, 699
DraIII CACNNNGTG 1 cut(s) 422
EaeI YGGCCR 1 cut(s) 618
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 8, 260
Eco57I CTGAAG 1 cut(s) 226
Eco81I CCTNAGG 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 144
EcoO109I RGGNCCY 2 cut(s) 141, 278
EcoRII CCWGG 2 cut(s) 39, 721
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 145
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 3 cut(s) 413, 440, 745
FaqI GGGAC 1 cut(s) 398
FatI CATG 3 cut(s) 409, 436, 741
FauNDI CATATG 2 cut(s) 545, 574
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 1 cut(s) 603
FokI GGATG 1 cut(s) 471
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 1 cut(s) 603
FspBI CTAG 3 cut(s) 77, 138, 153
GlaI GCGC 2 cut(s) 103, 354
GluI GCNGC 1 cut(s) 603
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 4 cut(s) 143, 279, 530, 620
HapII CCGG 2 cut(s) 113, 145
HgaI GACGC 1 cut(s) 23
HhaI GCGC 2 cut(s) 104, 355
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 413, 440, 745
Hin6I GCGC 2 cut(s) 102, 353
HinP1I GCGC 2 cut(s) 102, 353
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 2 cut(s) 94, 655
HpaII CCGG 2 cut(s) 113, 145
HphI GGTGA 2 cut(s) 371, 425
Hpy166II GTNNAC 3 cut(s) 238, 379, 737
Hpy188I TCNGA 2 cut(s) 257, 694
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 3 cut(s) 238, 379, 737
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 291, 580, 743
HpyCH4III ACNGT 2 cut(s) 129, 670
HpyCH4V TGCA 2 cut(s) 306, 425
HpyF3I CTNAG 2 cut(s) 211, 318
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 413, 440, 745
HspAI GCGC 2 cut(s) 102, 353
KasI GGCGCC 1 cut(s) 101
Kpn2I TCCGGA 1 cut(s) 112
Kzo9I GATC 3 cut(s) 33, 188, 699
LmnI GCTCC 2 cut(s) 322, 510
Lsp1109I GCAGC 1 cut(s) 589
MaeI CTAG 3 cut(s) 77, 138, 153
MalI GATC 3 cut(s) 35, 190, 701
MboI GATC 3 cut(s) 33, 188, 699
MboII GAAGA 2 cut(s) 76, 99
MflI RGATCY 3 cut(s) 33, 188, 699
MhlI GDGCHC 4 cut(s) 145, 151, 303, 327
MlsI TGGCCA 1 cut(s) 620
MluCI AATT 3 cut(s) 450, 470, 614
MluNI TGGCCA 1 cut(s) 620
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 734
Mox20I TGGCCA 1 cut(s) 620
MroI TCCGGA 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 620
MseI TTAA 2 cut(s) 474, 525
MslI CAYNNNNRTG 2 cut(s) 441, 577
Msp20I TGGCCA 1 cut(s) 620
MspCI CTTAAG 1 cut(s) 524
MspI CCGG 2 cut(s) 113, 145
MspR9I CCNGG 4 cut(s) 41, 145, 146, 723
Mva1269I GAATGC 1 cut(s) 584
MvaI CCWGG 2 cut(s) 41, 723
MvnI CGCG 1 cut(s) 353
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 2 cut(s) 145, 146
NcoI CCATGG 1 cut(s) 436
NdeI CATATG 2 cut(s) 545, 574
NdeII GATC 3 cut(s) 33, 188, 699
NlaIII CATG 3 cut(s) 413, 440, 745
NlaIV GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 217
NmeAIII GCCGAG 1 cut(s) 78
PctI GAATGC 1 cut(s) 584
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 655
PflFI GACNNNGTC 1 cut(s) 410
PflMI CCANNNNNTGG 1 cut(s) 269
PkrI GCNGC 1 cut(s) 604
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 88
PsiI TTATAA 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 39, 721
PspGI CCWGG 2 cut(s) 39, 721
PspN4I GGNNCC 6 cut(s) 10, 103, 143, 150, 190, 217
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 5 cut(s) 8, 141, 142, 260, 278
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 3 cut(s) 33, 188, 699
PsyI GACNNNGTC 1 cut(s) 410
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
RseI CAYNNNNRTG 2 cut(s) 441, 577
SalI GTCGAC 1 cut(s) 236
SaqAI TTAA 2 cut(s) 474, 525
SatI GCNGC 1 cut(s) 603
Sau3AI GATC 3 cut(s) 33, 188, 699
Sau96I GGNCC 5 cut(s) 8, 141, 142, 260, 278
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 4 cut(s) 41, 145, 146, 723
SduI GDGCHC 4 cut(s) 145, 151, 303, 327
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 2 cut(s) 8, 260
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 2 cut(s) 441, 577
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
SrfI GCCCGGGC 1 cut(s) 146
Sse9I AATT 3 cut(s) 450, 470, 614
SsiI CCGC 1 cut(s) 183
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 3 cut(s) 77, 138, 153
StyD4I CCNGG 4 cut(s) 39, 143, 144, 721
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 129, 670
TaqI TCGA 3 cut(s) 172, 237, 405
TasI AATT 3 cut(s) 450, 470, 614
TfiI GAWTC 1 cut(s) 655
Tru1I TTAA 2 cut(s) 474, 525
Tru9I TTAA 2 cut(s) 474, 525
TscAI CASTG 4 cut(s) 199, 270, 303, 525
TseI GCWGC 1 cut(s) 602
TspDTI ATGAA 6 cut(s) 63, 459, 483, 591, 678, 758
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 4 cut(s) 199, 270, 303, 525
Tth111I GACNNNGTC 1 cut(s) 410
Van91I CCANNNNNTGG 1 cut(s) 269
Vha464I CTTAAG 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 8, 260
XapI RAATTY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 3 cut(s) 77, 138, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.