FvH4_2g07543

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001194
Physical Location & Seq
Reverse (-)
6367602 .. 6369338
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07543.t1

Sequence Viewer

Length: 1230 bp
ATGTCGAACACACCTGTTGGACCAAATTTCGTCAATCTAGGGGATGAACCCACCATGCATGAACCCCTAAATTTGCTTCCTATTCAGGCTGAAGTGCCTTTCCCTGGAGGTCAATCTCTTAGGCTTTCTGCTACCCTTGGAGGTCTCTCCATAGCAGAAAAGCTTGAAGCTTTGAGAGACCTAGTTCCTTCTGCGGCTTCTGGTAAGCGGTCTCATTCTCCTTCCGGCGAAAATCCGGCCAAGGTTGCCAAGCCAGCCTCATCTGGTGGACCATCTGTGGTTCCCTCTCCTCCGTCGTCTCCGACTCGGTCTTCTCCTCACTCCACTGAGGTTGCATGGACAGAGGTGCCTGTCTTTGTCCCAGAGCCCAAATCCAAGCGTTGCACCACTAAACGTAGGGTTACCAGATCCACCTCGACTCCTCAGGTTCCCACTAAGCCTACTGGCGTTCCGGCTCTTGCTGAGGCCCCTCTTGCGGTCTCTGCTTCTGGGTCTTCGGTGGCTAAGGGTTTGTCGCCTCCTAAGGCTCCTGCCAAGCCCTCCGCTAGCTCTAAGCCCTCGCGTGCTGGTCCTCAAAATTCAGTTGACAAGGGCAAGGCTCCCATGGATGGAAGCAAGGCTCCTCAGTCCACCATGCCTCATAAGAAACGATCTGCTAAGGTATCTCACTCTTCAGTTCCTCCTCTTTCTGACTTTCAATCTGAGGCTGGTAGAGACTATTTCTGTACTTCCATTGTCAATAAGAGGATAGTGTTTGAGAGACCCATTGCTTTTGACACTTTGCATCCTGAGGCCATTCGTGGTGTGGGCACATCTCTTCGTGAGAATAGCTCTCTTGTCTTTCCTGCTGTCATCACTAAATTATGTTTGGATGCTGGTGTCCATGTCCTTGACTCTGATATCATGGCTAATGAAAATGAGGCTGCTCCGGTCGACAAGTTATCCTTGGCAAAATCAATGAGTCAGTCTGATGTGTATGTGGAGTCTTTGGAGCAGACCCTGCTGAAGCAAGAGATCAAGCGTCAAGTTTCCAATGATGTTGCTTCTCTTCAGGCTACCTTTACTGCTCAGTTTGAGGCCCTGCGAAATGAACTGAAAGAATCTGTGGGGCCACATGCGCCTCTTGCTAACCCCTCTGGTGGGGATAGCACCTCATCTACCCCTTCTTCCAGCTTTGGGTCTGAGGAGTCCTCTGATGCTTCTACTGCGGAGTCAGAGGAAGCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

410

Amino Acids

42.68

Weight (kDa)

7.1

Isoelectric Point (pI)

71.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccI GTMKAC 1 cut(s) 933
AccII CGCG 1 cut(s) 562
AciI CCGC 5 cut(s) 194, 208, 476, 543, 1208
AclWI GGATC 1 cut(s) 402
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 3 cut(s) 25, 70, 577
AcuI CTGAAG 4 cut(s) 111, 657, 1025, 1034
AfaI GTAC 1 cut(s) 727
AfiI CCNNNNNNNGG 6 cut(s) 104, 475, 608, 1139, 1140, 1176
AgsI TTSAA 2 cut(s) 167, 698
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 6 cut(s) 163, 170, 549, 831, 1173, 1223
AluI AGCT 6 cut(s) 163, 170, 549, 831, 1173, 1223
Alw26I GTCTC 7 cut(s) 149, 171, 216, 303, 484, 708, 754
AlwI GGATC 1 cut(s) 402
AlwNI CAGNNNCTG 1 cut(s) 1000
AoxI GGCC 5 cut(s) 237, 465, 792, 1077, 1109
ApeKI GCWGC 1 cut(s) 923
ApoI RAATTY 3 cut(s) 25, 70, 577
AspLEI GCGC 1 cut(s) 1120
AspS9I GGNCC 6 cut(s) 20, 269, 466, 569, 1078, 1109
AsuNHI GCTAGC 1 cut(s) 545
AvaII GGWCC 3 cut(s) 20, 269, 569
AxyI CCTNAGG 3 cut(s) 423, 522, 789
BaeGI GKGCMC 1 cut(s) 812
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 369
BbsI GAAGAC 2 cut(s) 303, 486
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 1 cut(s) 910
BccI CCATC 2 cut(s) 280, 602
BciT130I CCWGG 1 cut(s) 105
BcoDI GTCTC 7 cut(s) 149, 171, 216, 303, 484, 708, 754
BfaI CTAG 3 cut(s) 38, 182, 546
BisI GCNGC 2 cut(s) 195, 924
BlsI GCNGC 2 cut(s) 196, 925
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 3 cut(s) 20, 269, 569
BmgT120I GGNCC 6 cut(s) 20, 269, 466, 569, 1078, 1109
BmiI GGNNCC 8 cut(s) 282, 348, 429, 468, 528, 600, 621, 1110
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 3 cut(s) 793, 862, 1186
BmtI GCTAGC 1 cut(s) 549
BpiI GAAGAC 2 cut(s) 303, 486
BplI GAGNNNNNCTC 4 cut(s) 815, 847, 1175, 1207
BpmI CTGGAG 1 cut(s) 126
Bpu10I CCTNAGC 3 cut(s) 462, 504, 657
BsaI GGTCTC 5 cut(s) 149, 171, 216, 484, 754
BsaJI CCNNGG 5 cut(s) 103, 136, 240, 603, 945
BsaWI WCCGGW 1 cut(s) 928
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 6 cut(s) 104, 475, 608, 1139, 1140, 1176
Bse1I ACTGG 1 cut(s) 448
Bse21I CCTNAGG 3 cut(s) 423, 522, 789
Bse3DI GCAATG 1 cut(s) 765
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 5 cut(s) 103, 136, 240, 603, 945
BseGI GGATG 4 cut(s) 49, 613, 784, 877
BseLI CCNNNNNNNGG 6 cut(s) 104, 475, 608, 1139, 1140, 1176
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 8 cut(s) 318, 437, 453, 638, 693, 780, 1082, 1173
BseNI ACTGG 1 cut(s) 448
BseRI GAGGAG 6 cut(s) 279, 306, 411, 612, 672, 1199
BseSI GKGCMC 1 cut(s) 812
BseXI GCAGC 1 cut(s) 910
Bsh1236I CGCG 1 cut(s) 562
Bsh1285I CGRYCG 1 cut(s) 933
BshFI GGCC 5 cut(s) 239, 467, 794, 1079, 1111
BshNI GGYRCC 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 933
BsiSI CCGG 4 cut(s) 225, 236, 452, 929
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 6 cut(s) 104, 475, 608, 1139, 1140, 1176
BsmAI GTCTC 7 cut(s) 149, 171, 216, 303, 484, 708, 754
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 5 cut(s) 239, 467, 794, 1079, 1111
Bso31I GGTCTC 5 cut(s) 149, 171, 216, 484, 754
Bsp1286I GDGCHC 2 cut(s) 369, 812
Bsp143I GATC 3 cut(s) 407, 650, 1014
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 5 cut(s) 194, 208, 476, 543, 1208
BspANI GGCC 5 cut(s) 239, 467, 794, 1079, 1111
BspCNI CTCAG 8 cut(s) 319, 436, 454, 637, 694, 781, 1081, 1174
BspFNI CGCG 1 cut(s) 562
BspLI GGNNCC 8 cut(s) 282, 348, 429, 468, 528, 600, 621, 1110
BspOI GCTAGC 1 cut(s) 549
BspPI GGATC 1 cut(s) 402
BspT107I GGYRCC 1 cut(s) 346
BspTNI GGTCTC 5 cut(s) 149, 171, 216, 484, 754
BsrDI GCAATG 1 cut(s) 765
BsrI ACTGG 1 cut(s) 448
BssECI CCNNGG 5 cut(s) 103, 136, 240, 603, 945
BssMI GATC 3 cut(s) 407, 650, 1014
BssT1I CCWWGG 4 cut(s) 136, 240, 603, 945
Bst2UI CCWGG 1 cut(s) 105
Bst6I CTCTTC 3 cut(s) 676, 822, 1053
BstAPI GCANNNNNTGC 1 cut(s) 1000
BstC8I GCNNGC 3 cut(s) 255, 547, 564
BstDSI CCRYGG 1 cut(s) 603
BstEII GGTNACC 1 cut(s) 400
BstF5I GGATG 4 cut(s) 49, 613, 784, 877
BstFNI CGCG 1 cut(s) 562
BstHHI GCGC 1 cut(s) 1120
BstKTI GATC 3 cut(s) 410, 653, 1017
BstMAI GTCTC 7 cut(s) 149, 171, 216, 303, 484, 708, 754
BstMBI GATC 3 cut(s) 407, 650, 1014
BstMCI CGRYCG 1 cut(s) 933
BstMWI GCNNNNNNNGC 8 cut(s) 245, 254, 473, 482, 1000, 1117, 1124, 1205
BstNI CCWGG 1 cut(s) 105
BstNSI RCATGY 1 cut(s) 1118
BstPI GGTNACC 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 103
BstSLI GKGCMC 1 cut(s) 812
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 1 cut(s) 910
BstV2I GAAGAC 2 cut(s) 303, 486
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
Bsu36I CCTNAGG 3 cut(s) 423, 522, 789
BsuRI GGCC 5 cut(s) 239, 467, 794, 1079, 1111
BtgI CCRYGG 1 cut(s) 603
BtsCI GGATG 4 cut(s) 49, 613, 784, 877
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 3 cut(s) 255, 547, 564
CaiI CAGNNNCTG 1 cut(s) 1000
CfoI GCGC 1 cut(s) 1120
Cfr13I GGNCC 6 cut(s) 20, 269, 466, 569, 1078, 1109
CseI GACGC 1 cut(s) 1010
Csp6I GTAC 1 cut(s) 726
CspCI CAANNNNNGTGG 2 cut(s) 313, 348
CviAII CATG 8 cut(s) 55, 59, 336, 604, 634, 884, 904, 1115
CviQI GTAC 1 cut(s) 726
DpnI GATC 3 cut(s) 409, 652, 1016
DpnII GATC 3 cut(s) 407, 650, 1014
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 3 cut(s) 676, 822, 1053
EarI CTCTTC 3 cut(s) 676, 822, 1053
Eco130I CCWWGG 4 cut(s) 136, 240, 603, 945
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 5 cut(s) 149, 171, 216, 484, 754
Eco32I GATATC 1 cut(s) 901
Eco47I GGWCC 3 cut(s) 20, 269, 569
Eco57I CTGAAG 4 cut(s) 111, 657, 1025, 1034
Eco81I CCTNAGG 3 cut(s) 423, 522, 789
Eco91I GGTNACC 1 cut(s) 400
EcoO109I RGGNCCY 2 cut(s) 466, 1078
EcoO65I GGTNACC 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 103
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 4 cut(s) 136, 240, 603, 945
EcoT22I ATGCAT 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 4 cut(s) 136, 240, 603, 945
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 8 cut(s) 58, 62, 339, 607, 637, 887, 907, 1118
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 1 cut(s) 344
FatI CATG 8 cut(s) 54, 58, 335, 603, 633, 883, 903, 1114
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 2 cut(s) 195, 924
FokI GGATG 4 cut(s) 56, 620, 771, 884
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 2 cut(s) 195, 924
FspBI CTAG 3 cut(s) 38, 182, 546
GlaI GCGC 1 cut(s) 1119
GluI GCNGC 2 cut(s) 195, 924
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 5 cut(s) 239, 467, 794, 1079, 1111
HapII CCGG 4 cut(s) 225, 236, 452, 929
HgaI GACGC 1 cut(s) 1010
HhaI GCGC 1 cut(s) 1120
Hin1II CATG 8 cut(s) 58, 62, 339, 607, 637, 887, 907, 1118
Hin6I GCGC 1 cut(s) 1118
HinP1I GCGC 1 cut(s) 1118
HincII GTYRAC 2 cut(s) 586, 934
HindII GTYRAC 2 cut(s) 586, 934
HindIII AAGCTT 2 cut(s) 161, 168
HinfI GANTC 8 cut(s) 304, 418, 893, 961, 983, 1100, 1187, 1211
HpaII CCGG 4 cut(s) 225, 236, 452, 929
Hpy166II GTNNAC 4 cut(s) 269, 586, 630, 934
Hpy188I TCNGA 8 cut(s) 303, 691, 703, 898, 970, 1183, 1195, 1216
Hpy188III TCNNGA 2 cut(s) 788, 821
Hpy8I GTNNAC 4 cut(s) 269, 586, 630, 934
Hpy99I CGWCG 1 cut(s) 298
HpyAV CCTTC 3 cut(s) 198, 231, 1173
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 4 cut(s) 58, 335, 384, 784
HpyF10VI GCNNNNNNNGC 8 cut(s) 245, 254, 473, 482, 1000, 1117, 1124, 1205
HpySE526I ACGT 1 cut(s) 394
Hsp92II CATG 8 cut(s) 58, 62, 339, 607, 637, 887, 907, 1118
HspAI GCGC 1 cut(s) 1118
Kzo9I GATC 3 cut(s) 407, 650, 1014
LmnI GCTCC 5 cut(s) 532, 604, 625, 931, 991
Lsp1109I GCAGC 1 cut(s) 910
LweI GCATC 3 cut(s) 793, 862, 1186
MaeI CTAG 3 cut(s) 38, 182, 546
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 1 cut(s) 400
MalI GATC 3 cut(s) 409, 652, 1016
MboI GATC 3 cut(s) 407, 650, 1014
MboII GAAGA 6 cut(s) 303, 486, 663, 809, 1040, 1158
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 2 cut(s) 369, 812
MluCI AATT 4 cut(s) 25, 70, 577, 860
MlyI GAGTC 7 cut(s) 298, 412, 887, 970, 992, 1196, 1220
MmeI TCCRAC 1 cut(s) 326
Mph1103I ATGCAT 1 cut(s) 60
MspI CCGG 4 cut(s) 225, 236, 452, 929
MspR9I CCNGG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 8 cut(s) 245, 254, 473, 482, 1000, 1117, 1124, 1205
NcoI CCATGG 1 cut(s) 603
NdeII GATC 3 cut(s) 407, 650, 1014
NheI GCTAGC 1 cut(s) 545
NlaIII CATG 8 cut(s) 58, 62, 339, 607, 637, 887, 907, 1118
NlaIV GGNNCC 8 cut(s) 282, 348, 429, 468, 528, 600, 621, 1110
NsiI ATGCAT 1 cut(s) 60
NspI RCATGY 1 cut(s) 1118
PfeI GAWTC 1 cut(s) 1100
PflFI GACNNNGTC 1 cut(s) 307
PkrI GCNGC 2 cut(s) 196, 925
PleI GAGTC 7 cut(s) 298, 412, 887, 969, 991, 1195, 1219
PpsI GAGTC 7 cut(s) 298, 412, 887, 969, 991, 1195, 1219
Psp6I CCWGG 1 cut(s) 103
PspEI GGTNACC 1 cut(s) 400
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 8 cut(s) 282, 348, 429, 468, 528, 600, 621, 1110
PspPI GGNCC 6 cut(s) 20, 269, 466, 569, 1078, 1109
PstNI CAGNNNCTG 1 cut(s) 1000
PsuI RGATCY 1 cut(s) 407
PsyI GACNNNGTC 1 cut(s) 307
RsaI GTAC 1 cut(s) 727
RsaNI GTAC 1 cut(s) 726
SalI GTCGAC 1 cut(s) 932
SatI GCNGC 2 cut(s) 195, 924
Sau3AI GATC 3 cut(s) 407, 650, 1014
Sau96I GGNCC 6 cut(s) 20, 269, 466, 569, 1078, 1109
SchI GAGTC 7 cut(s) 298, 412, 887, 970, 992, 1196, 1220
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 2 cut(s) 369, 812
SfaNI GCATC 3 cut(s) 793, 862, 1186
SinI GGWCC 3 cut(s) 20, 269, 569
Sse9I AATT 4 cut(s) 25, 70, 577, 860
SsiI CCGC 5 cut(s) 194, 208, 476, 543, 1208
SspMI CTAG 3 cut(s) 38, 182, 546
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 4 cut(s) 136, 240, 603, 945
TaiI ACGT 1 cut(s) 397
TaqI TCGA 3 cut(s) 5, 416, 933
TaqII GACCGA 1 cut(s) 297
TasI AATT 4 cut(s) 25, 70, 577, 860
TatI WGTACW 1 cut(s) 725
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 1 cut(s) 1100
TscAI CASTG 1 cut(s) 331
TseI GCWGC 1 cut(s) 923
TspDTI ATGAA 4 cut(s) 60, 75, 927, 1104
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 331
Tth111I GACNNNGTC 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 20, 269, 569
XapI RAATTY 3 cut(s) 25, 70, 577
XceI RCATGY 1 cut(s) 1118
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XmiI GTMKAC 1 cut(s) 933
XspI CTAG 3 cut(s) 38, 182, 546
Zsp2I ATGCAT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.