FvH4_4g16891

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001194
Physical Location & Seq
Reverse (-)
20826660 .. 20829193
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16891.t1

Sequence Viewer

Length: 1155 bp
ATGATGTGTCCATATGAGTGTCCTTGTTACACATATTGTCCAAGGCTGAAGTGCCTATCTTTGGAGGTCCGGCTTACTGCTACCCCTGGCGGTCCCTCCATAGCAGACAAGCTTGAAGCTCTAAGAGACCTAGTTTCTCCTCCTGCTCTGGGTAAGAGGCCTCGCTCTCCCTCCGGTGAAAAACCGGTCAAGGTCGCTAAGCCATCTCTCTCCAGTGGTGGTGGGCCATCTGCGGCGGTTCCTTTTCCTCCATCCTCTCCGGTTAGGTCTCCAATTAGATCTCCCTCGCCTTCTTCTGAGGGTGTATGGACCGAGGTGCCTGTCCTAGCCCCTGAGCCCAAATCCAAGCGGTGCACCTCTAAACGTAGGGTTACCAGATCATCCTCTGCTCCTCAGGAGCCCTCTACACCAACTGGTGCTCCAGCTCCGGTTGAGACCTCCTCGGAGAATGCACCTGAAAAGGTGAAAATTCAATCTCAGTGCCCCATTGTTGTCAAGGTTAGGGGTAAGGATGTTGACATTTCTCCTCCTAGGATTCGATCTAGCATGTCTCTCCCTGCCACAAAATCCAACTCAGTCAAACTGGTTTTTGATTACATCAAATCTGTCTCTCTTCTTGAGCTAGGAGGAGCCCTTTATCAGTCCACTTTTCCTCGGCCAGAGATCACTGCTTCTCATCTTTCCAGTCAATTTATGTCTGAGCTGTGCAAGCTTTTAGGGTTCGTGGTTCGATCTCTTTTGACTCTCACCTCCAACACAGGCAAAATCACTCACCAACAGGCTGCCCTGATTAAATATCTGATTTCTAGCCCTTCCAAGCCTCGTTTTCCTGCTGAGTGTTTTATTTACAAAGCCATCAAGGGTGCGGGCACATCTCTTCGTGAGAATAGCTCTCTTGTCTTCCCTGCTGTCATCACAAAACTGTGTTTGGATGTTGGTGTACATGTCCTTGATGCTGATGTCATGGCTACTGAGAATGAGGCTGCTCCGGTCGACAAGCTATCCTTAGCAAAATCGATGAGCCAGTCTGATGTGTATGTGGAATCATTGGAGCAGACCCTGCTTAGCAAGAGATCAAACGTCAAGTTTCAAATGCTGTTGCTACTCTGCAGGCTTCTTTTATTGCTCAGTTTGAGACCCTGCGAAATGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

385

Amino Acids

41.39

Weight (kDa)

9.42

Isoelectric Point (pI)

70.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_32 PF20167 160 - 314 3e-11 Putative plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 316
AccI GTMKAC 1 cut(s) 993
AciI CCGC 5 cut(s) 90, 233, 236, 349, 866
AcoI YGGCCR 1 cut(s) 656
AcsI RAATTY 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 68
AfaI GTAC 1 cut(s) 942
AfiI CCNNNNNNNGG 2 cut(s) 61, 149
AflIII ACRYGT 1 cut(s) 943
AgeI ACCGGT 1 cut(s) 184
AgsI TTSAA 3 cut(s) 116, 473, 1091
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 8 cut(s) 112, 119, 425, 622, 703, 712, 891, 1000
AluI AGCT 8 cut(s) 112, 119, 425, 622, 703, 712, 891, 1000
Alw21I GWGCWC 2 cut(s) 356, 421
Alw26I GTCTC 6 cut(s) 120, 273, 428, 555, 613, 1129
Alw44I GTGCAC 1 cut(s) 352
AlwNI CAGNNNCTG 1 cut(s) 1060
AoxI GGCC 3 cut(s) 158, 224, 656
ApaLI GTGCAC 1 cut(s) 352
ApeKI GCWGC 2 cut(s) 782, 983
ApoI RAATTY 1 cut(s) 468
AsiGI ACCGGT 1 cut(s) 184
AspA2I CCTAGG 1 cut(s) 530
AspS9I GGNCC 4 cut(s) 67, 92, 224, 309
AsuHPI GGTGA 4 cut(s) 188, 475, 739, 764
AvaII GGWCC 3 cut(s) 67, 92, 309
AvrII CCTAGG 1 cut(s) 530
AxyI CCTNAGG 1 cut(s) 393
BaeGI GKGCMC 3 cut(s) 356, 485, 872
BanI GGYRCC 1 cut(s) 316
BanII GRGCYC 3 cut(s) 339, 402, 634
BbsI GAAGAC 1 cut(s) 892
Bbv12I GWGCWC 2 cut(s) 356, 421
BbvI GCAGC 2 cut(s) 769, 970
BccI CCATC 4 cut(s) 211, 235, 259, 863
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 6 cut(s) 120, 273, 428, 555, 613, 1129
BfaI CTAG 6 cut(s) 131, 326, 531, 543, 623, 807
BfmI CTRYAG 1 cut(s) 1108
BglII AGATCT 1 cut(s) 278
BisI GCNGC 3 cut(s) 234, 783, 984
BlnI CCTAGG 1 cut(s) 530
BlpI GCTNAGC 2 cut(s) 198, 1064
BlsI GCNGC 3 cut(s) 235, 784, 985
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 3 cut(s) 67, 92, 309
BmgT120I GGNCC 4 cut(s) 67, 92, 224, 309
BmiI GGNNCC 5 cut(s) 94, 240, 318, 399, 631
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 943
BpiI GAAGAC 1 cut(s) 892
BplI GAGNNNNNCTC 4 cut(s) 425, 457, 875, 907
BpmI CTGGAG 2 cut(s) 196, 405
Bpu10I CCTNAGC 2 cut(s) 333, 1006
Bpu1102I GCTNAGC 2 cut(s) 198, 1064
BpuEI CTTGAG 1 cut(s) 638
Bsa29I ATCGAT 1 cut(s) 1016
BsaI GGTCTC 4 cut(s) 120, 273, 428, 1129
BsaJI CCNNGG 6 cut(s) 41, 85, 312, 441, 530, 653
BsaWI WCCGGW 5 cut(s) 173, 184, 259, 427, 988
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 149
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 5 cut(s) 213, 418, 588, 684, 1024
Bse21I CCTNAGG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 87
BseCI ATCGAT 1 cut(s) 1016
BseDI CCNNGG 6 cut(s) 41, 85, 312, 441, 530, 653
BseGI GGATG 4 cut(s) 251, 380, 517, 937
BseLI CCNNNNNNNGG 2 cut(s) 61, 149
BseMII CTCAG 9 cut(s) 288, 324, 407, 491, 588, 690, 825, 963, 1141
BseNI ACTGG 5 cut(s) 213, 418, 588, 684, 1024
BseRI GAGGAG 5 cut(s) 129, 381, 430, 516, 642
BseSI GKGCMC 3 cut(s) 356, 485, 872
BseXI GCAGC 2 cut(s) 769, 970
Bsh1285I CGRYCG 1 cut(s) 993
BshFI GGCC 3 cut(s) 160, 226, 658
BshNI GGYRCC 1 cut(s) 316
BshTI ACCGGT 1 cut(s) 184
BshVI ATCGAT 1 cut(s) 1016
BsiEI CGRYCG 1 cut(s) 993
BsiHKAI GWGCWC 2 cut(s) 356, 421
BsiSI CCGG 6 cut(s) 70, 174, 185, 260, 428, 989
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 2 cut(s) 61, 149
BsmAI GTCTC 6 cut(s) 120, 273, 428, 555, 613, 1129
BsmFI GGGAC 1 cut(s) 78
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 3 cut(s) 160, 226, 658
Bso31I GGTCTC 4 cut(s) 120, 273, 428, 1129
Bsp1286I GDGCHC 7 cut(s) 339, 356, 402, 421, 485, 634, 872
Bsp1407I TGTACA 1 cut(s) 940
Bsp143I GATC 6 cut(s) 278, 377, 539, 663, 731, 1073
Bsp1720I GCTNAGC 2 cut(s) 198, 1064
BspACI CCGC 5 cut(s) 90, 233, 236, 349, 866
BspANI GGCC 3 cut(s) 160, 226, 658
BspCNI CTCAG 9 cut(s) 289, 325, 406, 490, 587, 691, 826, 964, 1140
BspDI ATCGAT 1 cut(s) 1016
BspLI GGNNCC 5 cut(s) 94, 240, 318, 399, 631
BspMAI CTGCAG 1 cut(s) 1112
BspT107I GGYRCC 1 cut(s) 316
BspTNI GGTCTC 4 cut(s) 120, 273, 428, 1129
BsrFI RCCGGY 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 940
BsrI ACTGG 5 cut(s) 213, 418, 588, 684, 1024
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 6 cut(s) 41, 85, 312, 441, 530, 653
BssMI GATC 6 cut(s) 278, 377, 539, 663, 731, 1073
BssT1I CCWWGG 2 cut(s) 41, 530
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 924
Bst6I CTCTTC 2 cut(s) 618, 882
BstAPI GCANNNNNTGC 1 cut(s) 1060
BstAUI TGTACA 1 cut(s) 940
BstC8I GCNNGC 3 cut(s) 710, 868, 1112
BstEII GGTNACC 1 cut(s) 370
BstF5I GGATG 4 cut(s) 251, 380, 517, 937
BstKTI GATC 6 cut(s) 281, 380, 542, 666, 734, 1076
BstMAI GTCTC 6 cut(s) 120, 273, 428, 555, 613, 1129
BstMBI GATC 6 cut(s) 278, 377, 539, 663, 731, 1073
BstMCI CGRYCG 1 cut(s) 993
BstMWI GCNNNNNNNGC 2 cut(s) 709, 1060
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 2 cut(s) 550, 947
BstPI GGTNACC 1 cut(s) 370
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 1108
BstSLI GKGCMC 3 cut(s) 356, 485, 872
BstV1I GCAGC 2 cut(s) 769, 970
BstV2I GAAGAC 1 cut(s) 892
BstX2I RGATCY 1 cut(s) 278
BstYI RGATCY 1 cut(s) 278
Bsu15I ATCGAT 1 cut(s) 1016
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 3 cut(s) 160, 226, 658
BsuTUI ATCGAT 1 cut(s) 1016
BtsCI GGATG 4 cut(s) 251, 380, 517, 937
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 220, 485, 666
Cac8I GCNNGC 3 cut(s) 710, 868, 1112
CaiI CAGNNNCTG 1 cut(s) 1060
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 4 cut(s) 67, 92, 224, 309
ClaI ATCGAT 1 cut(s) 1016
Csp6I GTAC 1 cut(s) 941
CspAI ACCGGT 1 cut(s) 184
CviAII CATG 3 cut(s) 547, 944, 964
CviQI GTAC 1 cut(s) 941
DpnI GATC 6 cut(s) 280, 379, 541, 665, 733, 1075
DpnII GATC 6 cut(s) 278, 377, 539, 663, 731, 1073
EaeI YGGCCR 1 cut(s) 656
Eam1104I CTCTTC 2 cut(s) 618, 882
EarI CTCTTC 2 cut(s) 618, 882
Eco130I CCWWGG 2 cut(s) 41, 530
Eco147I AGGCCT 1 cut(s) 160
Eco24I GRGCYC 3 cut(s) 339, 402, 634
Eco31I GGTCTC 4 cut(s) 120, 273, 428, 1129
Eco47I GGWCC 3 cut(s) 67, 92, 309
Eco57I CTGAAG 1 cut(s) 68
Eco81I CCTNAGG 1 cut(s) 393
Eco91I GGTNACC 1 cut(s) 370
EcoO65I GGTNACC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 41, 530
EcoT38I GRGCYC 3 cut(s) 339, 402, 634
ErhI CCWWGG 2 cut(s) 41, 530
FaeI CATG 3 cut(s) 550, 947, 967
FalI AAGNNNNNCTT 2 cut(s) 989, 1021
FaqI GGGAC 1 cut(s) 78
FatI CATG 3 cut(s) 546, 943, 963
FauI CCCGC 1 cut(s) 859
FauNDI CATATG 1 cut(s) 13
FblI GTMKAC 1 cut(s) 993
Fnu4HI GCNGC 3 cut(s) 234, 783, 984
FokI GGATG 4 cut(s) 238, 367, 524, 944
FriOI GRGCYC 3 cut(s) 339, 402, 634
Fsp4HI GCNGC 3 cut(s) 234, 783, 984
FspBI CTAG 6 cut(s) 131, 326, 531, 543, 623, 807
GluI GCNGC 3 cut(s) 234, 783, 984
GsuI CTGGAG 2 cut(s) 196, 405
HaeIII GGCC 3 cut(s) 160, 226, 658
HapII CCGG 6 cut(s) 70, 174, 185, 260, 428, 989
Hin1II CATG 3 cut(s) 550, 947, 967
HincII GTYRAC 2 cut(s) 517, 994
HindII GTYRAC 2 cut(s) 517, 994
HindIII AAGCTT 2 cut(s) 110, 710
HinfI GANTC 3 cut(s) 535, 742, 1043
HpaII CCGG 6 cut(s) 70, 174, 185, 260, 428, 989
HphI GGTGA 4 cut(s) 188, 475, 739, 764
Hpy166II GTNNAC 5 cut(s) 354, 517, 645, 941, 994
Hpy188I TCNGA 5 cut(s) 298, 445, 700, 801, 1030
Hpy188III TCNNGA 3 cut(s) 395, 617, 881
Hpy8I GTNNAC 5 cut(s) 354, 517, 645, 941, 994
HpyAV CCTTC 2 cut(s) 300, 822
HpyCH4III ACNGT 1 cut(s) 924
HpyCH4IV ACGT 2 cut(s) 364, 1080
HpyCH4V TGCA 4 cut(s) 354, 452, 708, 1110
HpyF10VI GCNNNNNNNGC 2 cut(s) 709, 1060
HpySE526I ACGT 2 cut(s) 364, 1080
Hsp92II CATG 3 cut(s) 550, 947, 967
Kzo9I GATC 6 cut(s) 278, 377, 539, 663, 731, 1073
LmnI GCTCC 7 cut(s) 394, 397, 424, 430, 629, 991, 1051
Lsp1109I GCAGC 2 cut(s) 769, 970
LweI GCATC 1 cut(s) 943
MaeI CTAG 6 cut(s) 131, 326, 531, 543, 623, 807
MaeII ACGT 2 cut(s) 364, 1080
MaeIII GTNAC 2 cut(s) 26, 370
MalI GATC 6 cut(s) 280, 379, 541, 665, 733, 1075
MboI GATC 6 cut(s) 278, 377, 539, 663, 731, 1073
MboII GAAGA 4 cut(s) 285, 605, 869, 892
MflI RGATCY 1 cut(s) 278
MhlI GDGCHC 7 cut(s) 339, 356, 402, 421, 485, 634, 872
MluCI AATT 3 cut(s) 273, 468, 689
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 2 cut(s) 594, 777
MseI TTAA 1 cut(s) 792
MslI CAYNNNNRTG 1 cut(s) 16
MspI CCGG 6 cut(s) 70, 174, 185, 260, 428, 989
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 709, 1060
NdeI CATATG 1 cut(s) 13
NdeII GATC 6 cut(s) 278, 377, 539, 663, 731, 1073
NlaIII CATG 3 cut(s) 550, 947, 967
NlaIV GGNNCC 5 cut(s) 94, 240, 318, 399, 631
NmeAIII GCCGAG 1 cut(s) 634
NspI RCATGY 2 cut(s) 550, 947
PceI AGGCCT 1 cut(s) 160
PciI ACATGT 1 cut(s) 943
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 2 cut(s) 535, 1043
PinAI ACCGGT 1 cut(s) 184
PkrI GCNGC 3 cut(s) 235, 784, 985
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PscI ACATGT 1 cut(s) 943
Psp6I CCWGG 1 cut(s) 85
PspEI GGTNACC 1 cut(s) 370
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 5 cut(s) 94, 240, 318, 399, 631
PspPI GGNCC 4 cut(s) 67, 92, 224, 309
PstI CTGCAG 1 cut(s) 1112
PstNI CAGNNNCTG 1 cut(s) 1060
PsuI RGATCY 1 cut(s) 278
RsaI GTAC 1 cut(s) 942
RsaNI GTAC 1 cut(s) 941
RseI CAYNNNNRTG 1 cut(s) 16
SalI GTCGAC 1 cut(s) 992
SaqAI TTAA 1 cut(s) 792
SatI GCNGC 3 cut(s) 234, 783, 984
Sau3AI GATC 6 cut(s) 278, 377, 539, 663, 731, 1073
Sau96I GGNCC 4 cut(s) 67, 92, 224, 309
SchI GAGTC 1 cut(s) 736
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 7 cut(s) 339, 356, 402, 421, 485, 634, 872
SfaNI GCATC 1 cut(s) 943
SfcI CTRYAG 1 cut(s) 1108
SinI GGWCC 3 cut(s) 67, 92, 309
SmiMI CAYNNNNRTG 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 3 cut(s) 273, 468, 689
SseBI AGGCCT 1 cut(s) 160
SsiI CCGC 5 cut(s) 90, 233, 236, 349, 866
SspMI CTAG 6 cut(s) 131, 326, 531, 543, 623, 807
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 2 cut(s) 41, 530
TaaI ACNGT 1 cut(s) 924
TaiI ACGT 2 cut(s) 367, 1083
TaqI TCGA 4 cut(s) 538, 730, 993, 1016
TaqII GACCGA 1 cut(s) 326
TasI AATT 3 cut(s) 273, 468, 689
TatI WGTACW 1 cut(s) 940
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 2 cut(s) 535, 1043
Tru1I TTAA 1 cut(s) 792
Tru9I TTAA 1 cut(s) 792
TscAI CASTG 3 cut(s) 220, 485, 673
TseI GCWGC 2 cut(s) 782, 983
TspRI CASTG 3 cut(s) 220, 485, 673
VneI GTGCAC 1 cut(s) 352
VpaK11BI GGWCC 3 cut(s) 67, 92, 309
XapI RAATTY 1 cut(s) 468
XceI RCATGY 2 cut(s) 550, 947
XmaJI CCTAGG 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 993
XspI CTAG 6 cut(s) 131, 326, 531, 543, 623, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.