Rh5AG258900

function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001194
Physical Location & Seq
Reverse (-)
33785646 .. 33787838
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG258900.1

Sequence Viewer

Length: 1101 bp
ATGAGTCCAAGGATTAAAGCTTCCTCCAAGGTGAGTAAGAAAAATACCATTGTGCGTACAGTTTGTCGGCGATCACCTTCTGTCACCAAACGTGTTCGTAACCAAAAAGTTAAGTTGTGCTATGAAACCAGCACTTTGAAACGTAACATCATCTGTGAGCGTCCTTTTGAACTAGAGGATGTTGGTGAAGATTTCACTAATATTATTAACAAACGTCAGTGGAATTCCCTGATAGATTATAATGATCCACCAAATGCCACTCTGGTCAAAGAATTCTATGCTCGTCTTGCTGAGGATACTGGTACCAATCAGGATAAAGTTTATGTCAGGAAAAAGTTGGTACCTTTTAACCCTGAGGTTATTCGATGTACACTCAATCTTCCTCCTTGTAGTTTACCCGACAGCTATGCCACATTCAAGAAAATGATTGACAGAAAGAAGCAGCACTTGCCTTCTGACCTTCGTGATTCTGGGACCGGTCATGGTGAAATGCCATCATCAAACTATGGATGTCTGACTGATCTCCATAGGACCCTTGCTCAGCTCAGTAGATCATCTATTTGGCCTTCTAGTCAAGTATCATGGCTTTCAAATCGTTTGACCTGTTTAATTTGGCTCATCACTCAAGACGCAGTTGAAATCCCCTTACATGAAATCATTTATGATTGGATCTGTAAAGCAGCCAAGAGCAAGAAGTCATGTTTGGGCTTTCCTTGTCTTATCACCAAAATTGCTCATGATGCAGGAGTTGCTTTCCGCGTGCACGATAAGTTTATGAAGCTTCCGGAGCCTGTTGACAGTGTCAATAAGGTCAAGAGTGATGCTCATATAAAATTTTCCAACTCAAATCGTTCTTCTAGTAGTGCCCCTGTTGCCTCTTCTGGTCTGCATGATGATGCTTCTTTAACTTCGAATCCAATGTTGTGCGACTTGCAGAGTTCAATTATCGCTGTGGAAAAGGGGATAGCTTGCCTTAAGAATGCTGTCTCTCATGTTACTCAAGATACCATAGGTGACAATATTACTGGTTTGACTACCCGCATGTCTGCTTTGAAAGCTGAGTTAATTTCCATTACTAACGCTGTCGCTCATGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

366

Amino Acids

40.72

Weight (kDa)

9.23

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_32 PF20167 71 - 250 1.2e-25 Putative plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
Acc65I GGTACC 2 cut(s) 302, 340
AccB1I GGYRCC 2 cut(s) 302, 340
AccII CGCG 1 cut(s) 759
AccIII TCCGGA 1 cut(s) 784
AciI CCGC 2 cut(s) 757, 1039
AclWI GGATC 2 cut(s) 239, 677
AcsI RAATTY 3 cut(s) 223, 272, 833
AfaI GTAC 4 cut(s) 58, 304, 342, 370
AflII CTTAAG 1 cut(s) 974
AflIII ACRYGT 1 cut(s) 91
AgeI ACCGGT 1 cut(s) 476
AgsI TTSAA 7 cut(s) 139, 170, 418, 591, 638, 942, 1054
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AluBI AGCT 6 cut(s) 20, 405, 544, 781, 968, 1058
AluI AGCT 6 cut(s) 20, 405, 544, 781, 968, 1058
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 1 cut(s) 991
Alw44I GTGCAC 1 cut(s) 761
AlwI GGATC 2 cut(s) 239, 677
Aor13HI TCCGGA 1 cut(s) 784
AoxI GGCC 1 cut(s) 563
ApaLI GTGCAC 1 cut(s) 761
ApeKI GCWGC 2 cut(s) 442, 680
ApoI RAATTY 3 cut(s) 223, 272, 833
AsiGI ACCGGT 1 cut(s) 476
Asp718I GGTACC 2 cut(s) 302, 340
AspS9I GGNCC 2 cut(s) 474, 531
AsuHPI GGTGA 7 cut(s) 43, 66, 76, 197, 497, 715, 1025
AsuII TTCGAA 1 cut(s) 911
AvaII GGWCC 2 cut(s) 474, 531
AxyI CCTNAGG 1 cut(s) 354
BaeGI GKGCMC 2 cut(s) 765, 868
BanI GGYRCC 2 cut(s) 302, 340
Bbv12I GWGCWC 1 cut(s) 765
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 2 cut(s) 454, 692
BccI CCATC 1 cut(s) 502
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciVI GTATCC 1 cut(s) 289
BcoDI GTCTC 1 cut(s) 991
BfaI CTAG 3 cut(s) 173, 570, 858
BfrI CTTAAG 1 cut(s) 974
BfuI GTATCC 1 cut(s) 289
BisI GCNGC 2 cut(s) 443, 681
BlpI GCTNAGC 1 cut(s) 540
BlsI GCNGC 2 cut(s) 444, 682
Bme18I GGWCC 2 cut(s) 474, 531
BmgT120I GGNCC 2 cut(s) 474, 531
BmiI GGNNCC 5 cut(s) 304, 342, 475, 533, 789
BmsI GCATC 3 cut(s) 730, 811, 886
BplI GAGNNNNNCTC 2 cut(s) 808, 840
Bpu10I CCTNAGC 1 cut(s) 291
Bpu1102I GCTNAGC 1 cut(s) 540
Bpu14I TTCGAA 1 cut(s) 911
BpuEI CTTGAG 2 cut(s) 609, 984
BsaJI CCNNGG 2 cut(s) 8, 27
BsaWI WCCGGW 2 cut(s) 476, 784
Bse118I RCCGGY 1 cut(s) 476
Bse1I ACTGG 2 cut(s) 304, 1030
Bse21I CCTNAGG 1 cut(s) 354
BseAI TCCGGA 1 cut(s) 784
BseDI CCNNGG 2 cut(s) 8, 27
BseGI GGATG 2 cut(s) 184, 515
BseMII CTCAG 5 cut(s) 282, 345, 554, 559, 1050
BseNI ACTGG 2 cut(s) 304, 1030
BseSI GKGCMC 2 cut(s) 765, 868
BseXI GCAGC 2 cut(s) 454, 692
Bsh1236I CGCG 1 cut(s) 759
BshFI GGCC 1 cut(s) 565
BshNI GGYRCC 2 cut(s) 302, 340
BshTI ACCGGT 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 765
BsiSI CCGG 2 cut(s) 477, 785
BslFI GGGAC 1 cut(s) 487
BsmAI GTCTC 1 cut(s) 991
BsmFI GGGAC 1 cut(s) 487
BsmI GAATGC 1 cut(s) 985
BsnI GGCC 1 cut(s) 565
Bsp119I TTCGAA 1 cut(s) 911
Bsp1286I GDGCHC 2 cut(s) 765, 868
Bsp13I TCCGGA 1 cut(s) 784
Bsp1407I TGTACA 1 cut(s) 368
Bsp143I GATC 5 cut(s) 71, 244, 520, 551, 669
Bsp1720I GCTNAGC 1 cut(s) 540
BspACI CCGC 2 cut(s) 757, 1039
BspANI GGCC 1 cut(s) 565
BspCNI CTCAG 5 cut(s) 283, 346, 553, 558, 1051
BspEI TCCGGA 1 cut(s) 784
BspFNI CGCG 1 cut(s) 759
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 5 cut(s) 304, 342, 475, 533, 789
BspPI GGATC 2 cut(s) 239, 677
BspT104I TTCGAA 1 cut(s) 911
BspT107I GGYRCC 2 cut(s) 302, 340
BspTI CTTAAG 1 cut(s) 974
BsrFI RCCGGY 1 cut(s) 476
BsrGI TGTACA 1 cut(s) 368
BsrI ACTGG 2 cut(s) 304, 1030
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 8, 27
BssMI GATC 5 cut(s) 71, 244, 520, 551, 669
BssT1I CCWWGG 2 cut(s) 8, 27
Bst4CI ACNGT 2 cut(s) 61, 800
Bst6I CTCTTC 1 cut(s) 883
BstAFI CTTAAG 1 cut(s) 974
BstAPI GCANNNNNTGC 2 cut(s) 448, 749
BstAUI TGTACA 1 cut(s) 368
BstBI TTCGAA 1 cut(s) 911
BstC8I GCNNGC 2 cut(s) 761, 970
BstDEI CTNAG 5 cut(s) 291, 354, 540, 545, 1059
BstF5I GGATG 2 cut(s) 184, 515
BstFNI CGCG 1 cut(s) 759
BstKTI GATC 5 cut(s) 74, 247, 523, 554, 672
BstMAI GTCTC 1 cut(s) 991
BstMBI GATC 5 cut(s) 71, 244, 520, 551, 669
BstMWI GCNNNNNNNGC 7 cut(s) 287, 448, 740, 749, 787, 872, 1055
BstNSI RCATGY 1 cut(s) 1045
BstSLI GKGCMC 2 cut(s) 765, 868
BstUI CGCG 1 cut(s) 759
BstV1I GCAGC 2 cut(s) 454, 692
BstX2I RGATCY 1 cut(s) 669
BstYI RGATCY 1 cut(s) 669
Bsu36I CCTNAGG 1 cut(s) 354
BsuI GTATCC 1 cut(s) 289
BsuRI GGCC 1 cut(s) 565
BtsCI GGATG 2 cut(s) 184, 515
BtsIMutI CAGTG 2 cut(s) 224, 805
Cac8I GCNNGC 2 cut(s) 761, 970
CciI TCATGA 1 cut(s) 736
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 2 cut(s) 474, 531
CseI GACGC 2 cut(s) 149, 638
Csp6I GTAC 4 cut(s) 57, 303, 341, 369
CspAI ACCGGT 1 cut(s) 476
CviAII CATG 9 cut(s) 482, 582, 650, 699, 737, 890, 992, 1042, 1091
CviQI GTAC 4 cut(s) 57, 303, 341, 369
DdeI CTNAG 5 cut(s) 291, 354, 540, 545, 1059
DpnI GATC 5 cut(s) 73, 246, 522, 553, 671
DpnII GATC 5 cut(s) 71, 244, 520, 551, 669
Eam1104I CTCTTC 1 cut(s) 883
EarI CTCTTC 1 cut(s) 883
Eco130I CCWWGG 2 cut(s) 8, 27
Eco47I GGWCC 2 cut(s) 474, 531
Eco81I CCTNAGG 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 531
EcoRI GAATTC 2 cut(s) 223, 272
EcoT14I CCWWGG 2 cut(s) 8, 27
ErhI CCWWGG 2 cut(s) 8, 27
FaeI CATG 9 cut(s) 485, 585, 653, 702, 740, 893, 995, 1045, 1094
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 1 cut(s) 487
FatI CATG 9 cut(s) 481, 581, 649, 698, 736, 889, 991, 1041, 1090
FauI CCCGC 1 cut(s) 1046
Fnu4HI GCNGC 2 cut(s) 443, 681
FokI GGATG 2 cut(s) 191, 522
Fsp4HI GCNGC 2 cut(s) 443, 681
FspBI CTAG 3 cut(s) 173, 570, 858
GluI GCNGC 2 cut(s) 443, 681
HaeIII GGCC 1 cut(s) 565
HapII CCGG 2 cut(s) 477, 785
HgaI GACGC 2 cut(s) 149, 638
Hin1II CATG 9 cut(s) 485, 585, 653, 702, 740, 893, 995, 1045, 1094
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 2 cut(s) 18, 779
HinfI GANTC 3 cut(s) 4, 467, 913
HpaII CCGG 2 cut(s) 477, 785
HphI GGTGA 7 cut(s) 43, 66, 76, 197, 497, 715, 1025
Hpy166II GTNNAC 4 cut(s) 371, 395, 763, 796
Hpy188I TCNGA 2 cut(s) 457, 516
Hpy8I GTNNAC 4 cut(s) 371, 395, 763, 796
HpyAV CCTTC 4 cut(s) 87, 462, 470, 576
HpyCH4III ACNGT 2 cut(s) 61, 800
HpyCH4IV ACGT 3 cut(s) 91, 142, 214
HpyCH4V TGCA 4 cut(s) 743, 763, 889, 934
HpyF10VI GCNNNNNNNGC 7 cut(s) 287, 448, 740, 749, 787, 872, 1055
HpyF3I CTNAG 5 cut(s) 291, 354, 540, 545, 1059
HpySE526I ACGT 3 cut(s) 91, 142, 214
Hsp92II CATG 9 cut(s) 485, 585, 653, 702, 740, 893, 995, 1045, 1094
Kpn2I TCCGGA 1 cut(s) 784
KpnI GGTACC 2 cut(s) 306, 344
Kzo9I GATC 5 cut(s) 71, 244, 520, 551, 669
LmnI GCTCC 1 cut(s) 787
Lsp1109I GCAGC 2 cut(s) 454, 692
LweI GCATC 3 cut(s) 730, 811, 886
MaeI CTAG 3 cut(s) 173, 570, 858
MaeII ACGT 3 cut(s) 91, 142, 214
MaeIII GTNAC 5 cut(s) 82, 98, 143, 994, 1013
MalI GATC 5 cut(s) 73, 246, 522, 553, 671
MboI GATC 5 cut(s) 71, 244, 520, 551, 669
MboII GAAGA 4 cut(s) 200, 371, 846, 870
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 2 cut(s) 765, 868
MluCI AATT 7 cut(s) 223, 272, 609, 729, 833, 942, 1065
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 864
MnlI CCTC 6 cut(s) 34, 169, 286, 349, 393, 886
MroI TCCGGA 1 cut(s) 784
MseI TTAA 8 cut(s) 15, 111, 207, 348, 608, 905, 975, 1064
MslI CAYNNNNRTG 1 cut(s) 894
MspCI CTTAAG 1 cut(s) 974
MspI CCGG 2 cut(s) 477, 785
Mva1269I GAATGC 1 cut(s) 985
MvnI CGCG 1 cut(s) 759
MwoI GCNNNNNNNGC 7 cut(s) 287, 448, 740, 749, 787, 872, 1055
NdeII GATC 5 cut(s) 71, 244, 520, 551, 669
NlaIII CATG 9 cut(s) 485, 585, 653, 702, 740, 893, 995, 1045, 1094
NlaIV GGNNCC 5 cut(s) 304, 342, 475, 533, 789
NmuCI GTSAC 2 cut(s) 82, 1013
NspI RCATGY 1 cut(s) 1045
NspV TTCGAA 1 cut(s) 911
PagI TCATGA 1 cut(s) 736
PctI GAATGC 1 cut(s) 985
PfeI GAWTC 2 cut(s) 467, 913
PflFI GACNNNGTC 1 cut(s) 800
PinAI ACCGGT 1 cut(s) 476
PkrI GCNGC 2 cut(s) 444, 682
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PpuMI RGGWCCY 1 cut(s) 531
PsiI TTATAA 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 531
PspN4I GGNNCC 5 cut(s) 304, 342, 475, 533, 789
PspPI GGNCC 2 cut(s) 474, 531
PspPPI RGGWCCY 1 cut(s) 531
PsuI RGATCY 1 cut(s) 669
PsyI GACNNNGTC 1 cut(s) 800
RsaI GTAC 4 cut(s) 58, 304, 342, 370
RsaNI GTAC 4 cut(s) 57, 303, 341, 369
RseI CAYNNNNRTG 1 cut(s) 894
SaqAI TTAA 8 cut(s) 15, 111, 207, 348, 608, 905, 975, 1064
SatI GCNGC 2 cut(s) 443, 681
Sau3AI GATC 5 cut(s) 71, 244, 520, 551, 669
Sau96I GGNCC 2 cut(s) 474, 531
SchI GAGTC 1 cut(s) 13
SduI GDGCHC 2 cut(s) 765, 868
SfaNI GCATC 3 cut(s) 730, 811, 886
SfuI TTCGAA 1 cut(s) 911
SinI GGWCC 2 cut(s) 474, 531
SmiMI CAYNNNNRTG 1 cut(s) 894
SmlI CTYRAG 3 cut(s) 624, 974, 999
SmoI CTYRAG 3 cut(s) 624, 974, 999
Sse9I AATT 7 cut(s) 223, 272, 609, 729, 833, 942, 1065
SsiI CCGC 2 cut(s) 757, 1039
SspI AATATT 2 cut(s) 202, 1021
SspMI CTAG 3 cut(s) 173, 570, 858
StyI CCWWGG 2 cut(s) 8, 27
TaaI ACNGT 2 cut(s) 61, 800
TaiI ACGT 3 cut(s) 94, 145, 217
TaqI TCGA 2 cut(s) 364, 911
TasI AATT 7 cut(s) 223, 272, 609, 729, 833, 942, 1065
TatI WGTACW 1 cut(s) 368
TfiI GAWTC 2 cut(s) 467, 913
Tru1I TTAA 8 cut(s) 15, 111, 207, 348, 608, 905, 975, 1064
Tru9I TTAA 8 cut(s) 15, 111, 207, 348, 608, 905, 975, 1064
TscAI CASTG 2 cut(s) 224, 805
TseFI GTSAC 2 cut(s) 82, 1013
TseI GCWGC 2 cut(s) 442, 680
Tsp45I GTSAC 2 cut(s) 82, 1013
TspDTI ATGAA 3 cut(s) 138, 666, 791
TspRI CASTG 2 cut(s) 224, 805
Tth111I GACNNNGTC 1 cut(s) 800
Vha464I CTTAAG 1 cut(s) 974
VneI GTGCAC 1 cut(s) 761
VpaK11BI GGWCC 2 cut(s) 474, 531
XapI RAATTY 3 cut(s) 223, 272, 833
XceI RCATGY 1 cut(s) 1045
XspI CTAG 3 cut(s) 173, 570, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.